Restriction map for
pFN21AB6248
(Linear) (Six-base) MAPSORT of: KIBB6248.seq Check: 7475 from: 1 to: 2043
KIBB6248.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 423 755 1233 2043
Size: 423 332 478 810
Fragments arranged by size:
810 478 423 332
AflIII A'CryG_T
Cuts at: 0 619 2043
Size: 619 1424
AgeI A'CCGG_T
Cuts at: 0 1935 2043
Size: 1935 108
AleI CACnn'nnGTG
Cuts at: 0 61 595 2043
Size: 61 534 1448
Fragments arranged by size:
1448 534 61
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 79 2043
Size: 79 1964
AlwNI CAG_nnn'CTG
Cuts at: 0 226 364 1626 2043
Size: 226 138 1262 417
Fragments arranged by size:
1262 417 226 138
ApaLI G'TGCA_C
Cuts at: 0 838 1006 2043
Size: 838 168 1037
Fragments arranged by size:
1037 838 168
ApoI r'AATT_y
Cuts at: 0 44 298 500 2043
Size: 44 254 202 1543
Fragments arranged by size:
1543 254 202 44
AvaI C'yCGr_G
Cuts at: 0 843 2043
Size: 843 1200
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 903 936 1071 1104 2043
Size: 903 33 135 33 939
Fragments arranged by size:
939 903 135 33 33
BamHI G'GATC_C
Cuts at: 0 668 2043
Size: 668 1375
BanI G'GyrC_C
Cuts at: 0 584 1443 1512 2043
Size: 584 859 69 531
Fragments arranged by size:
859 584 531 69
BanII G_rGCy'C
Cuts at: 0 37 2043
Size: 37 2006
BbsI GAAGACnn'nnnn_
Cuts at: 0 1932 2043
Size: 1932 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 1099 2043
Size: 1099 944
BglI GCCn_nnn'nGGC
Cuts at: 0 593 1579 2043
Size: 593 986 464
Fragments arranged by size:
986 593 464
BglII A'GATC_T
Cuts at: 0 701 2043
Size: 701 1342
Bme1580I G_kGCm'C
Cuts at: 0 842 1010 1448 1469 1517 2043
Size: 842 168 438 21 48 526
Fragments arranged by size:
842 526 438 168 48 21
BmgBI CAC'GTC
Cuts at: 0 620 1371 2043
Size: 620 751 672
Fragments arranged by size:
751 672 620
BmrI ACTGGGnnnn_n'
Cuts at: 0 1464 2043
Size: 1464 579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 2043
Size: 1050 993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 711 1041 1366 1423 1435 2043
Size: 278 433 330 325 57 12 608
Fragments arranged by size:
608 433 330 325 278 57 12
Bpu10I CC'TnA_GC
Cuts at: 0 1156 2043
Size: 1156 887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 2043
Size: 889 1154
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 2043
Size: 262 1781
BsaHI Gr'CG_yC
Cuts at: 0 767 2043
Size: 767 1276
BsaWI w'CCGG_w
Cuts at: 0 989 1935 2043
Size: 989 946 108
Fragments arranged by size:
989 946 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 2043
Size: 1039 30 974
Fragments arranged by size:
1039 974 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1935 1938 2043
Size: 1935 3 105
Fragments arranged by size:
1935 105 3
BseYI C'CCAG_C
Cuts at: 0 190 1147 1399 1456 1694 1955 2043
Size: 190 957 252 57 238 261 88
Fragments arranged by size:
957 261 252 238 190 88 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1080 1729 2043
Size: 870 210 649 314
Fragments arranged by size:
870 649 314 210
BsiEI CG_ry'CG
Cuts at: 0 1104 2043
Size: 1104 939
BsiHKAI G_wGCw'C
Cuts at: 0 842 1010 1036 2043
Size: 842 168 26 1007
Fragments arranged by size:
1007 842 168 26
BsmI GAATG_Cn'
Cuts at: 0 1045 2043
Size: 1045 998
Bsp1286I G_dGCh'C
Cuts at: 0 37 842 1010 1036 1448 1469 1517 2043
Size: 37 805 168 26 412 21 48 526
Fragments arranged by size:
805 526 412 168 48 37 26 21
BspEI T'CCGG_A
Cuts at: 0 989 2043
Size: 989 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 423 755 1233 2043
Size: 338 85 332 478 810
Fragments arranged by size:
810 478 338 332 85
BsrBI CCG'CTC
Cuts at: 0 489 684 2043
Size: 489 195 1359
Fragments arranged by size:
1359 489 195
BsrDI GCAATG_nn'
Cuts at: 0 882 954 1694 2043
Size: 882 72 740 349
Fragments arranged by size:
882 740 349 72
BsrFI r'CCGG_y
Cuts at: 0 1222 1238 1613 1698 1935 2043
Size: 1222 16 375 85 237 108
Fragments arranged by size:
1222 375 237 108 85 16
BssSI C'ACGA_G
Cuts at: 0 1081 1720 2043
Size: 1081 639 323
Fragments arranged by size:
1081 639 323
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1124 1512 1626 2043
Size: 1124 388 114 417
Fragments arranged by size:
1124 417 388 114
BstYI r'GATC_y
Cuts at: 0 668 701 1622 2043
Size: 668 33 921 421
Fragments arranged by size:
921 668 421 33
Bsu36I CC'TnA_GG
Cuts at: 0 664 1770 2043
Size: 664 1106 273
Fragments arranged by size:
1106 664 273
BtgI C'CryG_G
Cuts at: 0 335 1179 1763 2043
Size: 335 844 584 280
Fragments arranged by size:
844 584 335 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1304 1896 1910 2043
Size: 1304 592 14 133
Fragments arranged by size:
1304 592 133 14
BtsI GCAGTG_nn'
Cuts at: 0 1092 1275 1559 1922 2043
Size: 1092 183 284 363 121
Fragments arranged by size:
1092 363 284 183 121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 135 170 2043
Size: 135 35 1873
Fragments arranged by size:
1873 135 35
DraI TTT'AAA
Cuts at: 0 497 2043
Size: 497 1546
EaeI y'GGCC_r
Cuts at: 0 1247 1919 2043
Size: 1247 672 124
Fragments arranged by size:
1247 672 124
EarI CTCTTCn'nnn_
Cuts at: 0 1261 1479 1956 2043
Size: 1261 218 477 87
Fragments arranged by size:
1261 477 218 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 711 1041 1366 1423 1435 2043
Size: 278 433 330 325 57 12 608
Fragments arranged by size:
608 433 330 325 278 57 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1630 2043
Size: 1630 413
FspI TGC'GCA
Cuts at: 0 1075 2043
Size: 1075 968
HaeII r_GCGC'y
Cuts at: 0 797 1933 2043
Size: 797 1136 110
Fragments arranged by size:
1136 797 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 1478 1510 2043
Size: 1188 32 258 32 533
Fragments arranged by size:
1188 533 258 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 2043
Size: 995 1048
MscI TGG'CCA
Cuts at: 0 1249 1921 2043
Size: 1249 672 122
Fragments arranged by size:
1249 672 122
MslI CAynn'nnrTG
Cuts at: 0 61 527 595 835 1915 2043
Size: 61 466 68 240 1080 128
Fragments arranged by size:
1080 466 240 128 68 61
MspA1I CmG'CkG
Cuts at: 0 165 634 884 932 1549 2043
Size: 165 469 250 48 617 494
Fragments arranged by size:
617 494 469 250 165 48
NaeI GCC'GGC
Cuts at: 0 1240 1615 1700 2043
Size: 1240 375 85 343
Fragments arranged by size:
1240 375 343 85
NcoI C'CATG_G
Cuts at: 0 1179 2043
Size: 1179 864
NgoMIV G'CCGG_C
Cuts at: 0 1238 1613 1698 2043
Size: 1238 375 85 345
Fragments arranged by size:
1238 375 345 85
NspI r_CATG'y
Cuts at: 0 582 834 2043
Size: 582 252 1209
Fragments arranged by size:
1209 582 252
PasI CC'CwG_GG
Cuts at: 0 1603 2043
Size: 1603 440
PstI C_TGCA'G
Cuts at: 0 1129 1554 1622 2043
Size: 1129 425 68 421
Fragments arranged by size:
1129 425 421 68
PvuII CAG'CTG
Cuts at: 0 165 2043
Size: 165 1878
SfcI C'TryA_G
Cuts at: 0 789 1125 1550 1618 2043
Size: 789 336 425 68 425
Fragments arranged by size:
789 425 425 336 68
SmlI C'TyrA_G
Cuts at: 0 904 2043
Size: 904 1139
StuI AGG'CCT
Cuts at: 0 1847 2043
Size: 1847 196
StyI C'CwwG_G
Cuts at: 0 1179 1574 2043
Size: 1179 395 469
Fragments arranged by size:
1179 469 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 958 1732 2043
Size: 247 711 774 311
Fragments arranged by size:
774 711 311 247
TatI w'GTAC_w
Cuts at: 0 1346 2043
Size: 1346 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 2043
Size: 736 32 1275
Fragments arranged by size:
1275 736 32
XbaI T'CTAG_A
Cuts at: 0 1206 2043
Size: 1206 837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1789 2043
Size: 1789 254
XmnI GAAnn'nnTTC
Cuts at: 0 856 940 1174 1270 2043
Size: 856 84 234 96 773
Fragments arranged by size:
856 773 234 96 84
Enzymes that do cut:
AarI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbsI BciVI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EarI Eco57MI EcoNI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PstI PvuII SfcI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AloI ApaI AscI AseI AsiSI AvrII BbeI BbvCI
BcgI BclI BfrBI BlpI BmtI BsaAI BsaBI BsiWI
BsmBI BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I
ClaI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI XmaI ZraI