Restriction map for
pFN21AB6730
(Linear) (Six-base) MAPSORT of: KIBB6730.seq Check: 694 from: 1 to: 414
KIBB6730.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 174 414
Size: 174 240
ApaI G_GGCC'C
Cuts at: 0 45 414
Size: 45 369
BanI G'GyrC_C
Cuts at: 0 134 414
Size: 134 280
BanII G_rGCy'C
Cuts at: 0 45 286 413 414
Size: 45 241 127 1
Fragments arranged by size:
241 127 45 1
BbeI G_GCGC'C
Cuts at: 0 138 414
Size: 138 276
BbvCI CC'TCA_GC
Cuts at: 0 349 414
Size: 349 65
Bme1580I G_kGCm'C
Cuts at: 0 45 119 414
Size: 45 74 295
Fragments arranged by size:
295 74 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 362 414
Size: 362 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 414
Size: 160 254
Bpu10I CC'TnA_GC
Cuts at: 0 349 414
Size: 349 65
BsaHI Gr'CG_yC
Cuts at: 0 89 135 414
Size: 89 46 279
Fragments arranged by size:
279 89 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 414
Size: 79 30 305
Fragments arranged by size:
305 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 157 160 196 414
Size: 122 35 3 36 218
Fragments arranged by size:
218 122 36 35 3
BseYI C'CCAG_C
Cuts at: 0 44 261 354 390 394 414
Size: 44 217 93 36 4 20
Fragments arranged by size:
217 93 44 36 20 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 414
Size: 202 212
BsiHKAI G_wGCw'C
Cuts at: 0 127 413 414
Size: 127 286 1
Fragments arranged by size:
286 127 1
Bsp1286I G_dGCh'C
Cuts at: 0 45 119 127 286 413 414
Size: 45 74 8 159 127 1
Fragments arranged by size:
159 127 74 45 8 1
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 414
Size: 18 396
Bsu36I CC'TnA_GG
Cuts at: 0 332 414
Size: 332 82
BtgI C'CryG_G
Cuts at: 0 55 414
Size: 55 359
EaeI y'GGCC_r
Cuts at: 0 191 414
Size: 191 223
EcoICRI GAG'CTC
Cuts at: 0 411 414
Size: 411 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 414
Size: 160 254
EcoNI CCTnn'n_nnAGG
Cuts at: 0 104 142 414
Size: 104 38 272
Fragments arranged by size:
272 104 38
EcoO109I rG'GnC_Cy
Cuts at: 0 28 41 81 336 414
Size: 28 13 40 255 78
Fragments arranged by size:
255 78 40 28 13
FspI TGC'GCA
Cuts at: 0 163 414
Size: 163 251
HaeII r_GCGC'y
Cuts at: 0 51 138 312 414
Size: 51 87 174 102
Fragments arranged by size:
174 102 87 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 414
Size: 79 32 303
Fragments arranged by size:
303 79 32
KasI G'GCGC_C
Cuts at: 0 134 414
Size: 134 280
MscI TGG'CCA
Cuts at: 0 193 414
Size: 193 221
MspA1I CmG'CkG
Cuts at: 0 57 394 414
Size: 57 337 20
Fragments arranged by size:
337 57 20
NarI GG'CG_CC
Cuts at: 0 135 414
Size: 135 279
PasI CC'CwG_GG
Cuts at: 0 33 414
Size: 33 381
PspOMI G'GGCC_C
Cuts at: 0 41 414
Size: 41 373
PstI C_TGCA'G
Cuts at: 0 11 241 414
Size: 11 230 173
Fragments arranged by size:
230 173 11
PvuII CAG'CTG
Cuts at: 0 394 414
Size: 394 20
SacI G_AGCT'C
Cuts at: 0 413 414
Size: 413 1
SacII CC_GC'GG
Cuts at: 0 58 414
Size: 58 356
SfcI C'TryA_G
Cuts at: 0 7 237 414
Size: 7 230 177
Fragments arranged by size:
230 177 7
SfoI GGC'GCC
Cuts at: 0 136 414
Size: 136 278
StyI C'CwwG_G
Cuts at: 0 65 414
Size: 65 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 414
Size: 211 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 354 386 414
Size: 354 32 28
Fragments arranged by size:
354 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 414
Size: 135 279
Enzymes that do cut:
AlwNI ApaI BanI BanII BbeI BbvCI Bme1580I BmrI
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BstAPI Bsu36I BtgI EaeI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MscI MspA1I NarI
PasI PspOMI PstI PvuII SacI SacII SfcI SfoI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI