(Circular) (Six-base) MAPSORT of: pFN21AB6720.seq Check: 7192 from: 1 to: 5107 pFN21AB6720 5107 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2917 278 Size: 53 83 186 936 1381 2468 Fragments arranged by size: 2468 1381 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2450 1937 Size: 513 4594 Acc65I G'GTAC_C Cuts at: 2428 2428 Size: 5107 AclI AA'CG_TT Cuts at: 1563 3234 3607 1563 Size: 1671 373 3063 Fragments arranged by size: 3063 1671 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3170 4554 4822 1632 Size: 1538 1384 268 1917 Fragments arranged by size: 1917 1538 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4910 AflIII A'CryG_T Cuts at: 1203 4006 4983 1203 Size: 2803 977 1327 Fragments arranged by size: 2803 1327 977 AgeI A'CCGG_T Cuts at: 3938 3938 Size: 5107 AhdI GACnn_n'nnGTC Cuts at: 3836 3836 Size: 5107 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5107 AlwNI CAG_nnn'CTG Cuts at: 2850 4422 2850 Size: 1572 3535 ApaLI G'TGCA_C Cuts at: 3164 4320 3164 Size: 1156 3951 ApoI r'AATT_y Cuts at: 1493 2416 2642 2794 4903 1493 Size: 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 923 226 152 AseI AT'TA_AT Cuts at: 160 1032 3661 160 Size: 872 2629 1606 Fragments arranged by size: 2629 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5107 AvaI C'yCGr_G Cuts at: 1870 1943 2432 1870 Size: 73 489 4545 Fragments arranged by size: 4545 489 73 BamHI G'GATC_C Cuts at: 2437 2804 2437 Size: 367 4740 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2428 3883 618 Size: 325 217 627 641 1455 1842 Fragments arranged by size: 1842 1455 641 627 325 217 BanII G_rGCy'C Cuts at: 1391 2426 1391 Size: 1035 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3298 3332 1903 Size: 34 1361 34 3678 Fragments arranged by size: 3678 1361 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2999 4209 2999 Size: 1210 3897 BclI T'GATC_A Cuts at: 1548 2134 1548 Size: 586 4521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3718 136 Size: 107 122 71 631 605 2046 1525 Fragments arranged by size: 2046 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5102 5102 Size: 5107 BlpI GC'TnA_GC Cuts at: 2524 2524 Size: 5107 Bme1580I G_kGCm'C Cuts at: 1790 3168 4324 1790 Size: 1378 1156 2573 Fragments arranged by size: 2573 1378 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2838 3796 442 Size: 416 53 83 399 24 1421 958 1753 Fragments arranged by size: 1753 1421 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 3767 1239 Size: 1139 1389 2579 Fragments arranged by size: 2579 1389 1139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3232 4097 4395 3232 Size: 865 298 3944 Fragments arranged by size: 3944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3770 882 Size: 617 2271 2219 Fragments arranged by size: 2271 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2898 493 Size: 711 1694 2702 Fragments arranged by size: 2702 1694 711 BsaBI GATnn'nnATC Cuts at: 5101 5101 Size: 5107 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2914 3296 3931 275 Size: 53 83 186 936 1381 382 635 1451 Fragments arranged by size: 1451 1381 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3540 3938 4212 4359 4770 3540 Size: 398 274 147 411 3877 Fragments arranged by size: 3877 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4882 Fragments arranged by size: 4882 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5107 BseYI C'CCAG_C Cuts at: 4310 4806 4310 Size: 496 4611 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2557 3318 4346 1540 Size: 275 186 556 761 1028 2301 Fragments arranged by size: 2301 1028 761 556 275 186 BsiHKAI G_wGCw'C Cuts at: 3168 3253 4324 3168 Size: 85 1071 3951 Fragments arranged by size: 3951 1071 85 BsmI GAATG_Cn' Cuts at: 2618 2711 2618 Size: 93 5014 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 5107 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2426 3168 3253 4324 1391 Size: 399 636 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 399 85 BspEI T'CCGG_A Cuts at: 4770 4770 Size: 5107 BspHI T'CATG_A Cuts at: 2996 4726 2996 Size: 1730 3377 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2462 844 Size: 1618 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2994 1394 Size: 429 1171 3507 Fragments arranged by size: 3507 1171 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3602 3776 65 Size: 1183 2354 174 1396 Fragments arranged by size: 2354 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 3751 3938 1700 Size: 110 434 1507 187 2869 Fragments arranged by size: 2869 1507 434 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5107 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5107 BssSI C'ACGA_G Cuts at: 3167 4179 3167 Size: 1012 4095 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5107 BstBI TT'CG_AA Cuts at: 3911 3911 Size: 5107 BstEII G'GTnAC_C Cuts at: 1184 2263 2372 1184 Size: 1079 109 3919 Fragments arranged by size: 3919 1079 109 BstYI r'GATC_y Cuts at: 1972 2437 2804 3189 3206 4658 4744 4756 5102 Size: 465 367 385 17 1452 86 12 346 Cuts at: 5102 1972 Size: 1977 Fragments arranged by size: 1977 1452 465 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2317 513 Size: 552 1252 3303 Fragments arranged by size: 3303 1252 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4368 Fragments arranged by size: 4368 683 56 BtsI GCAGTG_nn' Cuts at: 2336 2623 3417 3437 2336 Size: 287 794 20 4006 Fragments arranged by size: 4006 794 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5107 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5072 DraI TTT'AAA Cuts at: 2411 2411 Size: 5107 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4114 809 Size: 507 2798 1802 Fragments arranged by size: 2798 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2554 3443 5072 Size: 54 1425 50 275 18 724 889 1629 Cuts at: 5072 8 Size: 43 Fragments arranged by size: 1629 1425 889 724 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2554 1537 Size: 275 742 4090 Fragments arranged by size: 4090 742 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3037 1353 Size: 1684 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3694 4068 4214 387 Size: 1064 138 2105 374 146 1280 Fragments arranged by size: 2105 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 3170 3767 4554 4822 1239 Size: 393 746 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 746 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 5107 EcoRI G'AATT_C Cuts at: 2416 2794 2416 Size: 378 4729 FspI TGC'GCA Cuts at: 3613 3613 Size: 5107 HaeII r_GCGC'y Cuts at: 1678 4254 1678 Size: 2576 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3748 3780 3822 3854 1373 Size: 32 135 32 2176 32 42 32 2626 Fragments arranged by size: 2626 2176 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2451 2697 669 Size: 988 281 513 246 3079 Fragments arranged by size: 3079 988 513 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5107 HpaI GTT'AAC Cuts at: 2697 2697 Size: 5107 KpnI G_GTAC'C Cuts at: 2432 2432 Size: 5107 MfeI C'AATT_G Cuts at: 2706 2706 Size: 5107 MluI A'CGCG_T Cuts at: 4983 4983 Size: 5107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 4221 4405 1305 Size: 880 2036 184 2007 Fragments arranged by size: 2036 2007 880 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3285 Fragments arranged by size: 3285 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3065 3424 3583 518 Size: 648 1899 359 159 2042 Fragments arranged by size: 2042 1899 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2319 2523 3200 4348 4593 1606 Size: 713 204 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 245 204 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4555 NdeI CA'TA_TG Cuts at: 387 387 Size: 5107 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5107 NotI GC'GGCC_GC Cuts at: 2554 2554 Size: 5107 NspI r_CATG'y Cuts at: 1132 1229 2252 2465 4010 1132 Size: 97 1023 213 1545 2229 Fragments arranged by size: 2229 1545 1023 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5107 PciI A'CATG_T Cuts at: 4006 4006 Size: 5107 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5107 PmeI GTTT'AAAC Cuts at: 2411 2411 Size: 5107 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3176 3208 4689 4721 3176 Size: 32 1481 32 3562 Fragments arranged by size: 3562 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5107 PsiI TTA'TAA Cuts at: 2677 2677 Size: 5107 PstI C_TGCA'G Cuts at: 830 2459 830 Size: 1629 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5107 SacII CC_GC'GG Cuts at: 2320 2320 Size: 5107 SalI G'TCGA_C Cuts at: 1936 2449 1936 Size: 513 4594 SbfI CC_TGCA'GG Cuts at: 2459 2459 Size: 5107 ScaI AGT'ACT Cuts at: 3355 5067 3355 Size: 1712 3395 SexAI A'CCwGG_T Cuts at: 2376 2376 Size: 5107 SfcI C'TryA_G Cuts at: 826 1045 2455 3590 4271 4462 826 Size: 219 1410 1135 681 191 1471 Fragments arranged by size: 1471 1410 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5107 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5107 SmaI CCC'GGG Cuts at: 1872 2434 1872 Size: 562 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3211 4112 4374 820 Size: 197 926 1268 901 262 1553 Fragments arranged by size: 1553 1268 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2898 493 Size: 2405 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5107 SphI G_CATG'C Cuts at: 1132 2465 1132 Size: 1333 3774 SspI AAT'ATT Cuts at: 5 52 3031 5 Size: 47 2979 2081 Fragments arranged by size: 2979 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5107 StyI C'CwwG_G Cuts at: 513 1065 2546 513 Size: 552 1481 3074 Fragments arranged by size: 3074 1481 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3134 3151 3304 3489 3934 325 Size: 1255 1554 17 153 185 445 1498 Fragments arranged by size: 1554 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 3353 5065 Size: 274 80 33 51 1443 246 1130 1712 Cuts at: 5065 96 Size: 138 Fragments arranged by size: 1712 1443 1130 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5067 5099 5067 Size: 32 5075 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5107 XbaI T'CTAG_A Cuts at: 2443 2443 Size: 5107 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5107 XmaI C'CCGG_G Cuts at: 1870 2432 1870 Size: 562 4545 XmnI GAAnn'nnTTC Cuts at: 3236 3236 Size: 5107 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2915 276 Size: 53 83 186 936 1381 2468 Fragments arranged by size: 2468 1381 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI