Restriction map for
pFN21AB6213
(Circular) (Six-base) MAPSORT of: pFN21AB6213.seq Check: 470 from: 1 to: 6709
pFN21AB6213 6709 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4519 278
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 3006 4052 1937
Size: 816 253 1046 4594
Fragments arranged by size:
4594 1046 816 253
Acc65I G'GTAC_C
Cuts at: 2761 3614 4030 2761
Size: 853 416 5440
Fragments arranged by size:
5440 853 416
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3000 3236 3312 3356 3729 3932 4772
Size: 641 727 236 76 44 373 203 840
Cuts at: 4772 6156 6424 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 840 727 641 373 268 236
203 76 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 2016 2928 5608 6585 1203
Size: 813 912 2680 977 1327
Fragments arranged by size:
2680 1327 977 912 813
AgeI A'CCGG_T
Cuts at: 2946 5540 2946
Size: 2594 4115
AhdI GACnn_n'nnGTC
Cuts at: 5438 5438
Size: 6709
AleI CACnn'nnGTG
Cuts at: 1166 2664 1166
Size: 1498 5211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2682 1184
Size: 1498 5211
AlwNI CAG_nnn'CTG
Cuts at: 2365 2675 4452 6024 2365
Size: 310 1777 1572 3050
Fragments arranged by size:
3050 1777 1572 310
ApaI G_GGCC'C
Cuts at: 3152 3850 3152
Size: 698 6011
ApaLI G'TGCA_C
Cuts at: 4766 5922 4766
Size: 1156 5553
ApoI r'AATT_y
Cuts at: 1493 4018 4244 4396 6505 1493
Size: 2525 226 152 2109 1697
Fragments arranged by size:
2525 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 2682 3246 3609 4034 1870
Size: 73 652 87 564 363 425 4545
Fragments arranged by size:
4545 652 564 425 363 87 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2745 2778 2745
Size: 33 6676
BamHI G'GATC_C
Cuts at: 4039 4406 4039
Size: 367 6342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 3251 3586 3614 4030
Size: 325 217 627 974 490 335 28 416
Cuts at: 4030 5485 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 974 627 490 416 335 325
217 28
BanII G_rGCy'C
Cuts at: 1391 2094 2623 3152 3850 4028 1391
Size: 703 529 529 698 178 4072
Fragments arranged by size:
4072 703 698 529 529 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2423 3224 928
Size: 971 524 801 4413
Fragments arranged by size:
4413 971 801 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4900 4934 1903
Size: 34 2963 34 3678
Fragments arranged by size:
3678 2963 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2704 3832 4601 5811 2704
Size: 1128 769 1210 3602
Fragments arranged by size:
3602 1210 1128 769
BclI T'GATC_A
Cuts at: 1548 2431 1548
Size: 883 5826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2819 3332
Size: 107 122 71 631 605 337 810 513
Cuts at: 3332 5320 136
Size: 1988 1525
Fragments arranged by size:
1988 1525 810 631 605 513 337 122
107 71
BglII A'GATC_T
Cuts at: 3571 6704 3571
Size: 3133 3576
BlpI GC'TnA_GC
Cuts at: 4126 4126
Size: 6709
Bme1580I G_kGCm'C
Cuts at: 1790 3152 3254 3850 4770 5926 1790
Size: 1362 102 596 920 1156 2573
Fragments arranged by size:
2573 1362 1156 920 596 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3671 4440 5398
Size: 416 53 83 399 24 2254 769 958
Cuts at: 5398 442
Size: 1753
Fragments arranged by size:
2254 1753 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 6709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4022 5369 1239
Size: 2783 1347 2579
Fragments arranged by size:
2783 2579 1347
Bpu10I CC'TnA_GC
Cuts at: 2742 2742
Size: 6709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 3253 3254 4834 5699 5997 2374
Size: 879 1 1580 865 298 3086
Fragments arranged by size:
3086 1580 879 865 298 1
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5372 882
Size: 617 3873 2219
Fragments arranged by size:
3873 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4500 493
Size: 711 3296 2702
Fragments arranged by size:
3296 2702 711
BsaBI GATnn'nnATC
Cuts at: 6703 6703
Size: 6709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4516 4898 5533 275
Size: 53 83 186 936 2983 382 635 1451
Fragments arranged by size:
2983 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2946 5142 5540 5814 5961 6372 2946
Size: 2196 398 274 147 411 3283
Fragments arranged by size:
3283 2196 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 2763 2792 2793
Size: 30 165 30 619 30 711 29 1
Cuts at: 2793 2822 1178
Size: 29 5065
Fragments arranged by size:
5065 711 619 165 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2849 1186
Size: 916 747 5046
Fragments arranged by size:
5046 916 747
BseYI C'CCAG_C
Cuts at: 3796 5912 6408 3796
Size: 2116 496 4097
Fragments arranged by size:
4097 2116 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2688 3430 3945 1163
Size: 573 696 256 742 515 3927
Fragments arranged by size:
3927 742 696 573 515 256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4159 4920 5948 1540
Size: 275 186 2158 761 1028 2301
Fragments arranged by size:
2301 2158 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 3491 4770 4855 5926 2094
Size: 1397 1279 85 1071 2877
Fragments arranged by size:
2877 1397 1279 1071 85
BsmI GAATG_Cn'
Cuts at: 4220 4313 4220
Size: 93 6616
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2623 3152 3254 3491 3850 4028
Size: 399 304 529 529 102 237 359 178
Cuts at: 4028 4770 4855 5926 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 529 529 399 359 304
237 178 102 85
BspEI T'CCGG_A
Cuts at: 6372 6372
Size: 6709
BspHI T'CATG_A
Cuts at: 4598 6328 4598
Size: 1730 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3967 4064 844
Size: 3123 97 3489
Fragments arranged by size:
3489 3123 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4596 1394
Size: 429 2773 3507
Fragments arranged by size:
3507 2773 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5204 5378 65
Size: 1183 3956 174 1396
Fragments arranged by size:
3956 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2565 2946 5353 5540 1700
Size: 110 755 381 2407 187 2869
Fragments arranged by size:
2869 2407 755 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6709
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6709
BssSI C'ACGA_G
Cuts at: 4769 5781 4769
Size: 1012 5697
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2675 794
Size: 1881 4828
BstBI TT'CG_AA
Cuts at: 5513 5513
Size: 6709
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6709
BstXI CCAn_nnnn'nTGG
Cuts at: 3343 3817 3343
Size: 474 6235
BstYI r'GATC_y
Cuts at: 1972 2472 3189 3571 4039 4406 4791 4808 6260
Size: 500 717 382 468 367 385 17 1452
Cuts at: 6260 6346 6358 6704 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 717 500 468 385 382 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3504 3504
Size: 6709
BtgI C'CryG_G
Cuts at: 513 1065 2004 2706 3255 513
Size: 552 939 702 549 3967
Fragments arranged by size:
3967 939 702 552 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5970
Fragments arranged by size:
5970 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 3170 3942 4225 5019 5039 2329
Size: 841 772 283 794 20 3999
Fragments arranged by size:
3999 841 794 772 283 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6674
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5716 809
Size: 507 4400 1802
Fragments arranged by size:
4400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3828 4156
Size: 54 1425 50 275 18 180 1818 328
Cuts at: 4156 5045 6674 8
Size: 889 1629 43
Fragments arranged by size:
1818 1629 1425 889 328 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4156 1537
Size: 275 2344 4090
Fragments arranged by size:
4090 2344 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2199 4639 1353
Size: 706 140 2440 3423
Fragments arranged by size:
3423 2440 706 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5296 5670 5816 387
Size: 1064 138 3707 374 146 1280
Fragments arranged by size:
3707 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 6709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 3000 3236 3312 3356 3729 3932
Size: 393 641 727 236 76 44 373 203
Cuts at: 3932 4022 4772 5369 6156 6424 1239
Size: 90 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 727 641 597 393 373
268 236 203 90 76 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2489 2719 2741 3854 2369
Size: 120 230 22 1113 5224
Fragments arranged by size:
5224 1113 230 120 22
EcoO109I rG'GnC_Cy
Cuts at: 2542 2839 3094 3148 3415 3846 3847 2542
Size: 297 255 54 267 431 1 5404
Fragments arranged by size:
5404 431 297 267 255 54 1
EcoRI G'AATT_C
Cuts at: 4018 4396 4018
Size: 378 6331
EcoRV GAT'ATC
Cuts at: 2350 2689 2350
Size: 339 6370
FspI TGC'GCA
Cuts at: 5215 5215
Size: 6709
HaeII r_GCGC'y
Cuts at: 1678 2245 3327 5856 1678
Size: 567 1082 2529 2531
Fragments arranged by size:
2531 2529 1082 567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2464 2483 2496
Size: 32 135 32 450 32 410 19 13
Cuts at: 2496 2515 2792 2824 3211 3243 3520 3552 5350
Size: 19 277 32 387 32 277 32 1798
Cuts at: 5350 5382 5424 5456 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1798 450 410 387 277 277 135
42 32 32 32 32 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 4053 4299 669
Size: 988 281 816 1299 246 3079
Fragments arranged by size:
3079 1299 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 2653 748
Size: 1905 4804
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KpnI G_GTAC'C
Cuts at: 2765 3618 4034 2765
Size: 853 416 5440
Fragments arranged by size:
5440 853 416
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 6585 6585
Size: 6709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3167 5823 6007 1305
Size: 1862 2656 184 2007
Fragments arranged by size:
2656 2007 1862 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3830 10
Size: 54 1425 343 1998 2889
Fragments arranged by size:
2889 1998 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2664 3050 3674 4667 5026 5185 518
Size: 648 1498 386 624 993 359 159 2042
Fragments arranged by size:
2042 1498 993 648 624 386 359 159
MspA1I CmG'CkG
Cuts at: 1606 2672 3643 3800 4125 4802 5950 6195 1606
Size: 1066 971 157 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 971 677 325 245 157
NcoI C'CATG_G
Cuts at: 513 1065 2004 2706 513
Size: 552 939 702 4516
Fragments arranged by size:
4516 939 702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6709
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6709
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 2020 2932 3163 3409 4067 5612 1132
Size: 97 791 912 231 246 658 1545 2229
Fragments arranged by size:
2229 1545 912 791 658 246 231 97
PasI CC'CwG_GG
Cuts at: 1348 2767 1348
Size: 1419 5290
PciI A'CATG_T
Cuts at: 2016 2928 5608 2016
Size: 912 2680 3117
Fragments arranged by size:
3117 2680 912
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2365 1108
Size: 1257 5452
PfoI T'CCnGG_A
Cuts at: 3999 3999
Size: 6709
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 6291 6323 4778
Size: 32 1481 32 5164
Fragments arranged by size:
5164 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 3415 2542
Size: 873 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6709
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PspOMI G'GGCC_C
Cuts at: 3148 3846 3148
Size: 698 6011
PstI C_TGCA'G
Cuts at: 830 4061 830
Size: 3231 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 2672 3643 3800 2672
Size: 971 157 5581
Fragments arranged by size:
5581 971 157
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 6709
SalI G'TCGA_C
Cuts at: 1936 2752 4051 1936
Size: 816 1299 4594
Fragments arranged by size:
4594 1299 816
SanDI GG'GwC_CC
Cuts at: 2542 2542
Size: 6709
SbfI CC_TGCA'GG
Cuts at: 4061 4061
Size: 6709
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SexAI A'CCwGG_T
Cuts at: 2070 2070
Size: 6709
SfcI C'TryA_G
Cuts at: 826 1045 4057 5192 5873 6064 826
Size: 219 3012 1135 681 191 1471
Fragments arranged by size:
3012 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 4036 1872
Size: 2164 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2353 3233 3268 4813 5714 5976
Size: 197 926 410 880 35 1545 901 262
Cuts at: 5976 820
Size: 1553
Fragments arranged by size:
1553 1545 926 901 880 410 262 197
35
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 4067 1132
Size: 2935 3774
SspI AAT'ATT
Cuts at: 5 52 4633 5
Size: 47 4581 2081
Fragments arranged by size:
4581 2081 47
StuI AGG'CCT
Cuts at: 1517 3736 1517
Size: 2219 4490
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 3428 4148 513
Size: 552 939 702 722 720 3074
Fragments arranged by size:
3074 939 722 720 702 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 4736 4753 4906 5091 5536 325
Size: 1255 738 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 738 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4955 6667 96
Size: 274 80 33 51 1443 2978 1712 138
Fragments arranged by size:
2978 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2308 2340 2763 2795 6669 6701 2308
Size: 32 423 32 3874 32 2316
Fragments arranged by size:
3874 2316 423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6709
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6709
XmaI C'CCGG_G
Cuts at: 1870 4034 1870
Size: 2164 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 2861 4838 2268
Size: 593 1977 4139
Fragments arranged by size:
4139 1977 593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4517 276
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI