Restriction map for
pFN21AB6212
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BcgI BclI
BfrI BglI BglII BlnI BsaI
BsiWI BsmI BspDI BspHI BsrDI
BssHII Bst1107I BstBI BstYI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FbaI FseI HpaI KspI MfeI
MflI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SspI
SwaI Tru9I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1018 GACGTC GACGT^C
AccI 241 GTCTAC GT^[AC][GT]AC
AccIII 891 TCCGGA T^CCGGA
AcsI 6 GAATTC [GA]^AATT[CT]
AocI 1242 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1816 AGCGCT AGC^GCT
ApoI 6 GAATTC [GA]^AATT[CT]
AviII 1811 TGCGCA TGC^GCA
BalI 788 TGGCCA TGG^CCA
BbeI 1189 GGCGCC GGCGC^C
BbrPI 196 CACGTG CAC^GTG
BbvCI 1311 GCTGAGG CCTCAGC(-5/-2)
BciVI 201 GTATCC GTATCC(6/5)
BfaI 622 CTAG C^TAG
BlpI 1536 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1536 GCTCAGC GC^T[ACGT]AGC
BsaBI 1293 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 891 TCCGGA T^CCGGA
BsiEI 1919 CGATCG CG[GA][CT]^CG
Bsp1407I 1040 TGTACA T^GTACA
BspEI 891 TCCGGA T^CCGGA
BspLU11I 1868 ACATGT A^CATGT
BsrGI 1040 TGTACA T^GTACA
BssSI 1584 CTCGTG CACGAG(-5/-1)
Bsu36I 1242 CCTCAGG CC^T[ACGT]AGG
CelII 1536 GCTCAGC GC^T[ACGT]AGC
DrdI 1690 GACAGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1160 CTCTTC CTCTTC(1/4)
Eco47III 1816 AGCGCT AGC^GCT
Eco81I 1242 CCTCAGG CC^T[ACGT]AGG
EcoRI 6 GAATTC G^AATTC
FspI 1811 TGCGCA TGC^GCA
HincII 1377 GTTGAC GT[CT]^[GA]AC
HindII 1377 GTTGAC GT[CT]^[GA]AC
HindIII 1210 AAGCTT A^AGCTT
KasI 1189 GGCGCC G^GCGCC
Ksp632I 1160 CTCTTC CTCTTC(1/4)
MaeI 622 CTAG C^TAG
MamI 1293 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 788 TGGCCA TGG^CCA
MroI 891 TCCGGA T^CCGGA
MscI 788 TGGCCA TGG^CCA
NaeI 1723 GCCGGC GCC^GGC
NarI 1189 GGCGCC GG^CGCC
NgoMIV 1723 GCCGGC G^CCGGC
PmaCI 196 CACGTG CAC^GTG
PmlI 196 CACGTG CAC^GTG
PvuI 1919 CGATCG CGAT^CG
SacI 1659 GAGCTC GAGCT^C
SbfI 2082 CCTGCAGG CCTGCA^GG
SexAI 1877 ACCTGGT A^CC[AT]GGT
SfoI 1189 GGCGCC GGC^GCC
SmaI 1496 CCCGGG CCC^GGG
SphI 1719 GCATGC GCATG^C
Sse8387I 2082 CCTGCAGG CCTGCA^GG
SspBI 1040 TGTACA T^GTACA
XcmI 479 CCAGACACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1496 CCCGGG C^CCGGG
XmaI 1496 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 168 GGTACC G^GTACC
824 GGTACC
AlwI 657 GATCC GGATC(4/5)
1794 GATCC
ApaI 128 GGGCCC GGGCC^C
2016 GGGCCC
Asp718I 168 GGTACC G^GTACC
824 GGTACC
AspI 235 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1000 GACGGCGTC
BsaAI 196 CACGTG [CT]AC^GT[GA]
220 TACGTG
BsaWI 891 TCCGGA [AT]^CCGG[AT]
1906 ACCGGA
BspMI 165 GCAGGT ACCTGC(4/8)
177 GCAGGT
BsrBI 504 CCGCTC CCGCTC(-3/-3)
20 GAGCGG
BstEII 348 GGTGACC G^GT[ACGT]ACC
1698 GGTCACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
1698 GGTCACC
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
1698 GGTCACC
KpnI 168 GGTACC GGTAC^C
824 GGTACC
NspI 1719 GCATGC [GA]CATG^[CT]
1868 ACATGT
PflFI 235 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1000 GACGGCGTC
PflMI 387 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1278 CCAGGCGCTGG
PleI 748 GACTC GAGTC(4/5)
564 GAGTC
PpuMI 1706 AGGTCCT [GA]G^G[AT]CC[CT]
1799 GGGACCT
PvuII 1033 CAGCTG CAG^CTG
1680 CAGCTG
StuI 1133 AGGCCT AGG^CCT
1580 AGGCCT
Tsp509I 7 AATT ^AATT
541 AATT
Tth111I 235 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1000 GACGGCGTC
Van91I 387 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1278 CCAGGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I