Restriction map for
pFN21AB6212
(Linear) (Six-base) MAPSORT of: KIBB6212.seq Check: 8649 from: 1 to: 2094
KIBB6212.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 168 2094
Size: 168 1926
AatII G_ACGT'C
Cuts at: 0 1022 2094
Size: 1022 1072
AccI GT'mk_AC
Cuts at: 0 242 2094
Size: 242 1852
Acc65I G'GTAC_C
Cuts at: 0 168 824 2094
Size: 168 656 1270
Fragments arranged by size:
1270 656 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1732 2094
Size: 760 972 362
Fragments arranged by size:
972 760 362
AfeI AGC'GCT
Cuts at: 0 1818 2094
Size: 1818 276
AflIII A'CryG_T
Cuts at: 0 197 1466 1514 1868 2094
Size: 197 1269 48 354 226
Fragments arranged by size:
1269 354 226 197 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 2094
Size: 1006 1088
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 2094
Size: 625 32 1437
Fragments arranged by size:
1437 625 32
AlwNI CAG_nnn'CTG
Cuts at: 0 531 1284 1455 1623 1710 2094
Size: 531 753 171 168 87 384
Fragments arranged by size:
753 531 384 171 168 87
ApaI G_GGCC'C
Cuts at: 0 132 2020 2094
Size: 132 1888 74
Fragments arranged by size:
1888 132 74
ApoI r'AATT_y
Cuts at: 0 6 2094
Size: 6 2088
AvaI C'yCGr_G
Cuts at: 0 283 853 958 1183 1496 2094
Size: 283 570 105 225 313 598
Fragments arranged by size:
598 570 313 283 225 105
BanI G'GyrC_C
Cuts at: 0 168 340 697 824 1189 2094
Size: 168 172 357 127 365 905
Fragments arranged by size:
905 365 357 172 168 127
BanII G_rGCy'C
Cuts at: 0 132 427 1663 2020 2045 2094
Size: 132 295 1236 357 25 49
Fragments arranged by size:
1236 357 295 132 49 25
BbeI G_GCGC'C
Cuts at: 0 1193 2094
Size: 1193 901
BbsI GAAGACnn'nnnn_
Cuts at: 0 847 945 1184 2094
Size: 847 98 239 910
Fragments arranged by size:
910 847 239 98
BbvCI CC'TCA_GC
Cuts at: 0 1312 2094
Size: 1312 782
BciVI GTATCCnnnnn_n'
Cuts at: 0 212 2094
Size: 212 1882
BlpI GC'TnA_GC
Cuts at: 0 1537 2094
Size: 1537 557
Bme1580I G_kGCm'C
Cuts at: 0 132 345 700 2020 2094
Size: 132 213 355 1320 74
Fragments arranged by size:
1320 355 213 132 74
BmgBI CAC'GTC
Cuts at: 0 951 1308 1469 1517 2094
Size: 951 357 161 48 577
Fragments arranged by size:
951 577 357 161 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 622 1319 2094
Size: 622 697 775
Fragments arranged by size:
775 697 622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 1599 2094
Size: 469 1130 495
Fragments arranged by size:
1130 495 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 508 1069 1125 1645 2094
Size: 420 88 561 56 520 449
Fragments arranged by size:
561 520 449 420 88 56
Bpu10I CC'TnA_GC
Cuts at: 0 1312 2094
Size: 1312 782
BsaAI yAC'GTr
Cuts at: 0 198 222 2094
Size: 198 24 1872
Fragments arranged by size:
1872 198 24
BsaBI GATnn'nnATC
Cuts at: 0 1297 2094
Size: 1297 797
BsaHI Gr'CG_yC
Cuts at: 0 236 1004 1019 1190 1430 1915 2024 2094
Size: 236 768 15 171 240 485 109 70
Fragments arranged by size:
768 485 240 236 171 109 70 15
BsaWI w'CCGG_w
Cuts at: 0 891 1906 2094
Size: 891 1015 188
Fragments arranged by size:
1015 891 188
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 224 254 625 655 850 880 2094
Size: 224 30 371 30 195 30 1214
Fragments arranged by size:
1214 371 224 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1128 1269 1618 2094
Size: 1128 141 349 476
Fragments arranged by size:
1128 476 349 141
BseYI C'CCAG_C
Cuts at: 0 173 304 693 966 1732 2094
Size: 173 131 389 273 766 362
Fragments arranged by size:
766 389 362 273 173 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 211 235 865 931 997 1591 1636
Size: 202 9 24 630 66 66 594 45
Cuts at: 1636 2094
Size: 458
Fragments arranged by size:
630 594 458 202 66 66 45 24
9
BsiEI CG_ry'CG
Cuts at: 0 1922 2094
Size: 1922 172
BsiHKAI G_wGCw'C
Cuts at: 0 617 1663 1889 2094
Size: 617 1046 226 205
Fragments arranged by size:
1046 617 226 205
BsmBI CGTCTCn'nnnn_
Cuts at: 0 227 438 1906 2094
Size: 227 211 1468 188
Fragments arranged by size:
1468 227 211 188
Bsp1286I G_dGCh'C
Cuts at: 0 132 345 427 617 700 1663 1889 2020
Size: 132 213 82 190 83 963 226 131
Cuts at: 2020 2045 2094
Size: 25 49
Fragments arranged by size:
963 226 213 190 132 131 83 82
49 25
BspEI T'CCGG_A
Cuts at: 0 891 2094
Size: 891 1203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 168 2094
Size: 156 12 1926
Fragments arranged by size:
1926 156 12
BsrBI CCG'CTC
Cuts at: 0 22 506 2094
Size: 22 484 1588
Fragments arranged by size:
1588 484 22
BsrFI r'CCGG_y
Cuts at: 0 186 761 827 1317 1723 2094
Size: 186 575 66 490 406 371
Fragments arranged by size:
575 490 406 371 186 66
BsrGI T'GTAC_A
Cuts at: 0 1040 2094
Size: 1040 1054
BssSI C'ACGA_G
Cuts at: 0 1584 2094
Size: 1584 510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 189 807 1761 2094
Size: 189 618 954 333
Fragments arranged by size:
954 618 333 189
BstEII G'GTnAC_C
Cuts at: 0 348 1698 2094
Size: 348 1350 396
Fragments arranged by size:
1350 396 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 727 1365 1686 2094
Size: 727 638 321 408
Fragments arranged by size:
727 638 408 321
Bsu36I CC'TnA_GG
Cuts at: 0 1243 2094
Size: 1243 851
BtgI C'CryG_G
Cuts at: 0 38 353 407 459 902 1787 2094
Size: 38 315 54 52 443 885 307
Fragments arranged by size:
885 443 315 307 54 52 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 494 2006 2094
Size: 494 1512 88
Fragments arranged by size:
1512 494 88
BtsI GCAGTG_nn'
Cuts at: 0 603 1009 1050 2070 2094
Size: 603 406 41 1020 24
Fragments arranged by size:
1020 603 406 41 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 1696 2094
Size: 1696 398
EaeI y'GGCC_r
Cuts at: 0 788 829 905 2094
Size: 788 41 76 1189
Fragments arranged by size:
1189 788 76 41
EarI CTCTTCn'nnn_
Cuts at: 0 1166 2094
Size: 1166 928
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1837 2094
Size: 1837 257
EcoICRI GAG'CTC
Cuts at: 0 1661 2094
Size: 1661 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 508 760 1069 1125 1645 1732 2094
Size: 420 88 252 309 56 520 87 362
Fragments arranged by size:
520 420 362 309 252 88 87 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 994 1201 1276 1486 2094
Size: 994 207 75 210 608
Fragments arranged by size:
994 608 210 207 75
EcoO109I rG'GnC_Cy
Cuts at: 0 128 330 1026 1707 1800 2094
Size: 128 202 696 681 93 294
Fragments arranged by size:
696 681 294 202 128 93
EcoRI G'AATT_C
Cuts at: 0 6 2094
Size: 6 2088
FspI TGC'GCA
Cuts at: 0 1813 2094
Size: 1813 281
HaeII r_GCGC'y
Cuts at: 0 973 1193 1285 1464 1820 2094
Size: 973 220 92 179 356 274
Fragments arranged by size:
973 356 274 220 179 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 224 256 850 882 1936 1968 2094
Size: 224 32 594 32 1054 32 126
Fragments arranged by size:
1054 594 224 126 32 32 32
HincII GTy'rAC
Cuts at: 0 1379 2094
Size: 1379 715
HindIII A'AGCT_T
Cuts at: 0 1210 2094
Size: 1210 884
KasI G'GCGC_C
Cuts at: 0 1189 2094
Size: 1189 905
KpnI G_GTAC'C
Cuts at: 0 172 828 2094
Size: 172 656 1266
Fragments arranged by size:
1266 656 172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 2094
Size: 515 1579
MscI TGG'CCA
Cuts at: 0 790 2094
Size: 790 1304
MslI CAynn'nnrTG
Cuts at: 0 406 725 790 907 2094
Size: 406 319 65 117 1187
Fragments arranged by size:
1187 406 319 117 65
MspA1I CmG'CkG
Cuts at: 0 376 553 863 875 1035 1620 1682 2094
Size: 376 177 310 12 160 585 62 412
Fragments arranged by size:
585 412 376 310 177 160 62 12
NaeI GCC'GGC
Cuts at: 0 1725 2094
Size: 1725 369
NarI GG'CG_CC
Cuts at: 0 1190 2094
Size: 1190 904
NcoI C'CATG_G
Cuts at: 0 407 902 1787 2094
Size: 407 495 885 307
Fragments arranged by size:
885 495 407 307
NgoMIV G'CCGG_C
Cuts at: 0 1723 2094
Size: 1723 371
NspI r_CATG'y
Cuts at: 0 1723 1872 2094
Size: 1723 149 222
Fragments arranged by size:
1723 222 149
PciI A'CATG_T
Cuts at: 0 1868 2094
Size: 1868 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 1284 2094
Size: 393 891 810
Fragments arranged by size:
891 810 393
PfoI T'CCnGG_A
Cuts at: 0 1796 2094
Size: 1796 298
PmlI CAC'GTG
Cuts at: 0 198 2094
Size: 198 1896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 625 657 1855 1887 2094
Size: 625 32 1198 32 207
Fragments arranged by size:
1198 625 207 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1707 1800 2094
Size: 1707 93 294
Fragments arranged by size:
1707 294 93
PspOMI G'GGCC_C
Cuts at: 0 128 2016 2094
Size: 128 1888 78
Fragments arranged by size:
1888 128 78
PstI C_TGCA'G
Cuts at: 0 770 998 1034 1511 1652 2087 2094
Size: 770 228 36 477 141 435 7
Fragments arranged by size:
770 477 435 228 141 36 7
PvuI CG_AT'CG
Cuts at: 0 1922 2094
Size: 1922 172
PvuII CAG'CTG
Cuts at: 0 1035 1682 2094
Size: 1035 647 412
Fragments arranged by size:
1035 647 412
SacI G_AGCT'C
Cuts at: 0 1663 2094
Size: 1663 431
SbfI CC_TGCA'GG
Cuts at: 0 2087 2094
Size: 2087 7
SexAI A'CCwGG_T
Cuts at: 0 1877 2094
Size: 1877 217
SfcI C'TryA_G
Cuts at: 0 766 994 1030 1064 1507 1648 2083 2094
Size: 766 228 36 34 443 141 435 11
Fragments arranged by size:
766 443 435 228 141 36 34 11
SfoI GGC'GCC
Cuts at: 0 1191 2094
Size: 1191 903
SmaI CCC'GGG
Cuts at: 0 1498 2094
Size: 1498 596
SphI G_CATG'C
Cuts at: 0 1723 2094
Size: 1723 371
SrfI GCCC'GGGC
Cuts at: 0 1498 2094
Size: 1498 596
StuI AGG'CCT
Cuts at: 0 1135 1582 2094
Size: 1135 447 512
Fragments arranged by size:
1135 512 447
StyI C'CwwG_G
Cuts at: 0 407 902 1202 1421 1742 1787 2094
Size: 407 495 300 219 321 45 307
Fragments arranged by size:
495 407 321 307 300 219 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1578 1717 2026 2094
Size: 1578 139 309 68
Fragments arranged by size:
1578 309 139 68
TatI w'GTAC_w
Cuts at: 0 782 932 1040 1865 2094
Size: 782 150 108 825 229
Fragments arranged by size:
825 782 229 150 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1328 1360 2094
Size: 1146 32 150 32 734
Fragments arranged by size:
1146 734 150 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 238 1003 2094
Size: 238 765 1091
Fragments arranged by size:
1091 765 238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 486 2094
Size: 486 1608
XmaI C'CCGG_G
Cuts at: 0 1496 2094
Size: 1496 598
ZraI GAC'GTC
Cuts at: 0 1020 2094
Size: 1020 1074
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AlfI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BciVI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI Bsu36I
BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII SacI SbfI
SexAI SfcI SfoI SmaI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AleI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BclI BfrBI BglI
BglII BmtI BpuEI BsaI BsiWI BsmI BspHI BsrDI
BssHII BstBI BstYI BstZ17I ClaI CspCI DraI DraIII
EagI EcoRV FalI FseI FspAI HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI
SapI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SspI SwaI XbaI XhoI XmnI