Restriction map for
pFN21AB6193
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaI BseAI BsiEI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII BstBI BstEII BstPI BstUI
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 1961 GGCGCC G[GA]^CG[CT]C
BanI 1961 GGCGCC G^G[CT][GA]CC
BbeI 1961 GGCGCC GGCGC^C
BcgI 293 GCAGCGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1793 TGATCA T^GATCA
BfaI 184 CTAG C^TAG
BlpI 867 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 867 GCTGAGC GC^T[ACGT]AGC
BsaHI 1961 GGCGCC G[GA]^CG[CT]C
BsaWI 1092 ACCGGA [AT]^CCGG[AT]
BsgI 123 GTGCAG GTGCAG(16/14)
BsiWI 1820 CGTACG C^GTACG
BsmI 441 GAATGC GAATGC(1/-1)
BspHI 474 TCATGA T^CATGA
Bst1107I 1609 GTATAC GTA^TAC
BstAPI 1851 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 653 CCAGTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1609 GTATAC GTA^TAC
CelII 867 GCTGAGC GC^T[ACGT]AGC
DraII 197 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1420 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1824 CGGCCA [CT]^GGCC[GA]
EcoNI 810 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 197 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1718 CCATGG C^C[AT][AT]GG
FbaI 1793 TGATCA T^GATCA
HaeII 1961 GGCGCC [GA]GCGC^[CT]
HgaI 1950 GACGC GACGC(5/10)
Hin1I 1961 GGCGCC G[GA]^CG[CT]C
HincII 1084 GTCGAC GT[CT]^[GA]AC
HindII 1084 GTCGAC GT[CT]^[GA]AC
KasI 1961 GGCGCC G^GCGCC
MaeI 184 CTAG C^TAG
NarI 1961 GGCGCC GG^CGCC
NcoI 1718 CCATGG C^CATGG
PflMI 696 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 197 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 474 TCATGA T^CATGA
SalI 1084 GTCGAC G^TCGAC
SapI 1396 GAAGAGC GCTCTTC(1/4)
SbfI 1857 CCTGCAGG CCTGCA^GG
SexAI 1604 ACCAGGT A^CC[AT]GGT
SfoI 1961 GGCGCC GGC^GCC
Sse8387I 1857 CCTGCAGG CCTGCA^GG
StyI 1718 CCATGG C^C[AT][AT]GG
Van91I 696 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AseI 427 ATTAAT AT^TAAT
1135 ATTAAT
AsnI 427 ATTAAT AT^TAAT
1135 ATTAAT
Asp700I 897 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1195 GAAGACATTC
BglII 899 AGATCT A^GATCT
1727 AGATCT
BsrBI 1894 GAGCGG CCGCTC(-3/-3)
1933 GAGCGG
BsrDI 666 CATTGC GCAATG(2/0)
1622 CATTGC
BsrFI 1401 GCCGGC [GA]^CCGG[CT]
1964 GCCGGC
BssSI 1225 CACGAG CACGAG(-5/-1)
799 CTCGTG
Cfr10I 1401 GCCGGC [GA]^CCGG[CT]
1964 GCCGGC
DsaI 953 CCACGG C^C[GA][CT]GG
1718 CCATGG
MaeII 785 ACGT A^CGT
1819 ACGT
NaeI 1401 GCCGGC GCC^GGC
1964 GCCGGC
NgoMIV 1401 GCCGGC G^CCGGC
1964 GCCGGC
PshBI 427 ATTAAT AT^TAAT
1135 ATTAAT
TfiI 467 GAATC G^A[AT]TC
731 GAATC
Tsp45I 304 GTGAC ^GT[GC]AC
1426 GTGAC
XcmI 308 CCAGATGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
548 CCAGAGTGGCTGTGG
XmnI 897 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1195 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII