(Circular) (Six-base) MAPSORT of: pFN21AB6193.seq Check: 7820 from: 1 to: 6691 pFN21AB6193 6691 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3090 3597 3615 4034 1937 Size: 1153 507 18 419 4594 Fragments arranged by size: 4594 1153 507 419 18 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2397 2793 2837 3824 3914 3953 4754 Size: 725 40 396 44 987 90 39 801 Cuts at: 4754 6138 6406 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 987 801 725 396 268 90 44 40 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 5420 5420 Size: 6691 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6691 AlwNI CAG_nnn'CTG Cuts at: 2048 2206 3745 3862 4434 6006 2048 Size: 158 1539 117 572 1572 2733 Fragments arranged by size: 2733 1572 1539 572 158 117 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 3285 3308 3475 4000 4226 4378 6487 1493 Size: 1792 23 167 525 226 152 2109 1697 Fragments arranged by size: 2109 1792 1697 525 226 167 152 23 AseI AT'TA_AT Cuts at: 160 1032 2433 3141 5245 160 Size: 872 1401 708 2104 1606 Fragments arranged by size: 2104 1606 1401 872 708 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2245 2508 2714 4016 1870 Size: 73 302 263 206 1302 4545 Fragments arranged by size: 4545 1302 302 263 206 73 BamHI G'GATC_C Cuts at: 4021 4388 4021 Size: 367 6324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3966 4012 5467 618 Size: 325 217 627 2179 46 1455 1842 Fragments arranged by size: 2179 1842 1455 627 325 217 46 BanII G_rGCy'C Cuts at: 1391 2188 2246 2258 2997 4010 1391 Size: 797 58 12 739 1013 4072 Fragments arranged by size: 4072 1013 797 739 58 12 BbeI G_GCGC'C Cuts at: 3970 3970 Size: 6691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2781 3108 3207 3531 928 Size: 971 882 327 99 324 4088 Fragments arranged by size: 4088 971 882 327 324 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2287 2321 4882 4916 1903 Size: 34 350 34 2561 34 3678 Fragments arranged by size: 3678 2561 350 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4583 5793 4583 Size: 1210 5481 BclI T'GATC_A Cuts at: 1548 3798 1548 Size: 2250 4441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2904 3732 6686 2904 Size: 828 2954 2909 Fragments arranged by size: 2954 2909 828 BlpI GC'TnA_GC Cuts at: 2873 4108 2873 Size: 1235 5456 Bme1580I G_kGCm'C Cuts at: 1790 4752 5908 1790 Size: 2962 1156 2573 Fragments arranged by size: 2962 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2791 1318 Size: 1473 5218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 3973 4422 Size: 416 53 83 399 24 1235 1321 449 Cuts at: 4422 5380 442 Size: 958 1753 Fragments arranged by size: 1753 1321 1235 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2521 2521 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2963 3766 3803 5351 1239 Size: 1277 447 803 37 1548 2579 Fragments arranged by size: 2579 1548 1277 803 447 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4816 5681 5979 4816 Size: 865 298 5528 Fragments arranged by size: 5528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5354 882 Size: 617 3855 2219 Fragments arranged by size: 3855 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 6685 6685 Size: 6691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3967 4498 4880 5515 Size: 53 83 186 936 2434 531 382 635 Cuts at: 5515 275 Size: 1451 Fragments arranged by size: 2434 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3097 5124 5522 5796 5943 6354 3097 Size: 2027 398 274 147 411 3434 Fragments arranged by size: 3434 2027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 1178 Size: 30 165 30 855 30 5581 Fragments arranged by size: 5581 855 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 3083 3743 1186 Size: 970 927 660 4134 Fragments arranged by size: 4134 970 927 660 BseYI C'CCAG_C Cuts at: 5894 6390 5894 Size: 496 6195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2149 1163 Size: 573 413 5705 Fragments arranged by size: 5705 573 413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2246 2258 2997 4752 4837 5908 2246 Size: 12 739 1755 85 1071 3029 Fragments arranged by size: 3029 1755 1071 739 85 12 BsiWI C'GTAC_G Cuts at: 3825 3825 Size: 6691 BsmI GAATG_Cn' Cuts at: 2452 4202 4295 2452 Size: 1750 93 4848 Fragments arranged by size: 4848 1750 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2246 2258 2997 4010 4752 4837 Size: 399 398 58 12 739 1013 742 85 Cuts at: 4837 5908 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1013 742 739 399 398 85 58 12 BspEI T'CCGG_A Cuts at: 6354 6354 Size: 6691 BspHI T'CATG_A Cuts at: 2479 4580 6310 2479 Size: 2101 1730 2860 Fragments arranged by size: 2860 2101 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4046 844 Size: 3202 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3901 3940 4578 1394 Size: 429 2078 39 638 3507 Fragments arranged by size: 3507 2078 638 429 39 BsrDI GCAATG_nn' Cuts at: 65 1248 2670 3626 5186 5360 65 Size: 1183 1422 956 1560 174 1396 Fragments arranged by size: 1560 1422 1396 1183 956 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3406 3969 5335 5522 1700 Size: 110 1596 563 1366 187 2869 Fragments arranged by size: 2869 1596 1366 563 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 2804 3230 4751 5763 2804 Size: 426 1521 1012 3732 Fragments arranged by size: 3732 1521 1012 426 BstAPI GCAn_nnn'nTGC Cuts at: 794 3862 794 Size: 3068 3623 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstXI CCAn_nnnn'nTGG Cuts at: 2665 2665 Size: 6691 BstYI r'GATC_y Cuts at: 1972 2521 2859 2904 2935 3732 4021 4388 4773 Size: 549 338 45 31 797 289 367 385 Cuts at: 4773 4790 6242 6328 6340 6686 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 797 549 385 367 346 338 289 86 45 31 17 12 BstZ17I GTA'TAC Cuts at: 3616 3616 Size: 6691 BtgI C'CryG_G Cuts at: 513 1065 2004 2958 3723 513 Size: 552 939 954 765 3481 Fragments arranged by size: 3481 954 939 765 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2011 2206 2437 487 Size: 683 56 785 195 231 4741 Fragments arranged by size: 4741 785 683 231 195 56 BtsI GCAGTG_nn' Cuts at: 3162 4207 5001 5021 3162 Size: 1045 794 20 4832 Fragments arranged by size: 4832 1045 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DraIII CAC_nnn'GTG Cuts at: 3430 3430 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3829 4138 5027 Size: 54 1425 50 275 18 1999 309 889 Cuts at: 5027 6656 8 Size: 1629 43 Fragments arranged by size: 1999 1629 1425 889 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3396 3477 3724 4621 1353 Size: 2043 81 247 897 3423 Fragments arranged by size: 3423 2043 897 247 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3557 5278 5652 5798 387 Size: 1064 138 1968 1721 374 146 1280 Fragments arranged by size: 1968 1721 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2244 2256 2995 2244 Size: 12 739 5940 Fragments arranged by size: 5940 739 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2397 2516 2793 2837 2963 3766 Size: 393 725 40 119 277 44 126 803 Cuts at: 3766 3803 3824 3914 3953 4754 5351 6138 6406 Size: 37 21 90 39 801 597 787 268 Cuts at: 6406 1239 Size: 1524 Fragments arranged by size: 1524 803 801 787 725 597 393 277 268 126 119 90 44 40 39 37 21 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 6691 EcoO109I rG'GnC_Cy Cuts at: 2203 2203 Size: 6691 EcoRI G'AATT_C Cuts at: 4000 4378 4000 Size: 378 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2926 2926 Size: 6691 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 3970 5838 1678 Size: 2292 1868 2531 Fragments arranged by size: 2531 2292 1868 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2258 2260 2290 2358 Size: 32 135 32 656 30 2 30 68 Cuts at: 2358 2390 2889 2921 3739 3771 3946 3978 5332 Size: 32 499 32 818 32 175 32 1354 Cuts at: 5332 5364 5406 5438 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1354 818 656 499 175 135 68 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3091 4035 4281 669 Size: 988 281 1153 944 246 3079 Fragments arranged by size: 3079 1153 988 944 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6691 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KasI G'GCGC_C Cuts at: 3966 3966 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 3250 5805 5989 1305 Size: 939 1006 2555 184 2007 Fragments arranged by size: 2555 2007 1006 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4869 Fragments arranged by size: 4869 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4649 5008 5167 518 Size: 648 3483 359 159 2042 Fragments arranged by size: 3483 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2301 2323 2872 3885 4107 4784 5932 Size: 439 256 22 549 1013 222 677 1148 Cuts at: 5932 6177 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1013 677 549 439 256 245 222 22 NaeI GCC'GGC Cuts at: 3408 3971 3408 Size: 563 6128 NarI GG'CG_CC Cuts at: 3967 3967 Size: 6691 NcoI C'CATG_G Cuts at: 513 1065 2004 3723 513 Size: 552 939 1719 3481 Fragments arranged by size: 3481 1719 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NgoMIV G'CCGG_C Cuts at: 3406 3969 3406 Size: 563 6128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 4049 5594 1132 Size: 97 2820 1545 2229 Fragments arranged by size: 2820 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6691 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 5092 PfoI T'CCnGG_A Cuts at: 2517 2862 2517 Size: 345 6346 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2203 Size: 6691 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 4261 4261 Size: 6691 PstI C_TGCA'G Cuts at: 830 2895 3858 3867 4043 830 Size: 2065 963 9 176 3478 Fragments arranged by size: 3478 2065 963 176 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2045 2872 3885 2045 Size: 827 1013 4851 Fragments arranged by size: 4851 1013 827 SacI G_AGCT'C Cuts at: 2246 2258 2997 2246 Size: 12 739 5940 Fragments arranged by size: 5940 739 12 SalI G'TCGA_C Cuts at: 1936 3089 4033 1936 Size: 1153 944 4594 Fragments arranged by size: 4594 1153 944 SapI GCTCTTCn'nnn_ Cuts at: 3396 3396 Size: 6691 SbfI CC_TGCA'GG Cuts at: 3867 4043 3867 Size: 176 6515 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SexAI A'CCwGG_T Cuts at: 3609 3609 Size: 6691 SfcI C'TryA_G Cuts at: 826 1045 2891 3189 3854 3863 3880 4039 5174 Size: 219 1846 298 665 9 17 159 1135 Cuts at: 5174 5855 6046 826 Size: 681 191 1471 Fragments arranged by size: 1846 1471 1135 681 665 298 219 191 159 17 9 SfoI GGC'GCC Cuts at: 3968 3968 Size: 6691 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4795 5696 5958 820 Size: 197 926 2852 901 262 1553 Fragments arranged by size: 2852 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 4615 5 Size: 47 4563 2081 Fragments arranged by size: 4563 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2004 3723 4130 513 Size: 552 939 1719 407 3074 Fragments arranged by size: 3074 1719 939 552 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2937 4718 4735 4888 5073 5518 325 Size: 1255 1357 1781 17 153 185 445 1498 Fragments arranged by size: 1781 1498 1357 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 3248 4937 Size: 274 80 33 51 1443 639 632 1689 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1712 1689 1443 639 632 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6651 6683 6651 Size: 32 6659 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2560 2320 Size: 240 6451 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 2906 3204 4820 2906 Size: 298 1616 4777 Fragments arranged by size: 4777 1616 298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsmBI Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI