Restriction map for
pFN21AB6193
(Linear) (Six-base) MAPSORT of: KIBB6193.seq Check: 9071 from: 1 to: 1986
KIBB6193.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1085 1592 1610 1986
Size: 1085 507 18 376
Fragments arranged by size:
1085 507 376 18
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 392 788 832 1819 1909 1948 1986
Size: 352 40 396 44 987 90 39 38
Fragments arranged by size:
987 396 352 90 44 40 39 38
AlwNI CAG_nnn'CTG
Cuts at: 0 43 201 1740 1857 1986
Size: 43 158 1539 117 129
Fragments arranged by size:
1539 158 129 117 43
ApoI r'AATT_y
Cuts at: 0 1280 1303 1470 1986
Size: 1280 23 167 516
Fragments arranged by size:
1280 516 167 23
AseI AT'TA_AT
Cuts at: 0 428 1136 1986
Size: 428 708 850
Fragments arranged by size:
850 708 428
AvaI C'yCGr_G
Cuts at: 0 240 503 709 1986
Size: 240 263 206 1277
Fragments arranged by size:
1277 263 240 206
BanI G'GyrC_C
Cuts at: 0 1961 1986
Size: 1961 25
BanII G_rGCy'C
Cuts at: 0 183 241 253 992 1986
Size: 183 58 12 739 994
Fragments arranged by size:
994 739 183 58 12
BbeI G_GCGC'C
Cuts at: 0 1965 1986
Size: 1965 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 1103 1202 1526 1986
Size: 776 327 99 324 460
Fragments arranged by size:
776 460 327 324 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 282 316 1986
Size: 282 34 1670
Fragments arranged by size:
1670 282 34
BclI T'GATC_A
Cuts at: 0 1793 1986
Size: 1793 193
BglII A'GATC_T
Cuts at: 0 899 1727 1986
Size: 899 828 259
Fragments arranged by size:
899 828 259
BlpI GC'TnA_GC
Cuts at: 0 868 1986
Size: 868 1118
BmgBI CAC'GTC
Cuts at: 0 786 1986
Size: 786 1200
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 1968 1986
Size: 647 1321 18
Fragments arranged by size:
1321 647 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 516 1986
Size: 516 1470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 958 1761 1798 1986
Size: 511 447 803 37 188
Fragments arranged by size:
803 511 447 188 37
BsaHI Gr'CG_yC
Cuts at: 0 1962 1986
Size: 1962 24
BsaWI w'CCGG_w
Cuts at: 0 1092 1986
Size: 1092 894
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 1986
Size: 253 30 1703
Fragments arranged by size:
1703 253 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 1078 1738 1986
Size: 151 927 660 248
Fragments arranged by size:
927 660 248 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1986
Size: 144 1842
BsiHKAI G_wGCw'C
Cuts at: 0 241 253 992 1986
Size: 241 12 739 994
Fragments arranged by size:
994 739 241 12
BsiWI C'GTAC_G
Cuts at: 0 1820 1986
Size: 1820 166
BsmI GAATG_Cn'
Cuts at: 0 447 1986
Size: 447 1539
Bsp1286I G_dGCh'C
Cuts at: 0 183 241 253 992 1986
Size: 183 58 12 739 994
Fragments arranged by size:
994 739 183 58 12
BspHI T'CATG_A
Cuts at: 0 474 1986
Size: 474 1512
BsrBI CCG'CTC
Cuts at: 0 1896 1935 1986
Size: 1896 39 51
Fragments arranged by size:
1896 51 39
BsrDI GCAATG_nn'
Cuts at: 0 665 1621 1986
Size: 665 956 365
Fragments arranged by size:
956 665 365
BsrFI r'CCGG_y
Cuts at: 0 1401 1964 1986
Size: 1401 563 22
Fragments arranged by size:
1401 563 22
BssSI C'ACGA_G
Cuts at: 0 799 1225 1986
Size: 799 426 761
Fragments arranged by size:
799 761 426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1857 1986
Size: 1857 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 1986
Size: 660 1326
BstYI r'GATC_y
Cuts at: 0 516 854 899 930 1727 1986
Size: 516 338 45 31 797 259
Fragments arranged by size:
797 516 338 259 45 31
BstZ17I GTA'TAC
Cuts at: 0 1611 1986
Size: 1611 375
BtgI C'CryG_G
Cuts at: 0 953 1718 1986
Size: 953 765 268
Fragments arranged by size:
953 765 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6 201 432 1986
Size: 6 195 231 1554
Fragments arranged by size:
1554 231 195 6
BtsI GCAGTG_nn'
Cuts at: 0 1157 1986
Size: 1157 829
DraIII CAC_nnn'GTG
Cuts at: 0 1425 1986
Size: 1425 561
EaeI y'GGCC_r
Cuts at: 0 1824 1986
Size: 1824 162
EarI CTCTTCn'nnn_
Cuts at: 0 1391 1472 1719 1986
Size: 1391 81 247 267
Fragments arranged by size:
1391 267 247 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1552 1986
Size: 1552 434
EcoICRI GAG'CTC
Cuts at: 0 239 251 990 1986
Size: 239 12 739 996
Fragments arranged by size:
996 739 239 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 392 511 788 832 958 1761 1798
Size: 352 40 119 277 44 126 803 37
Cuts at: 1798 1819 1909 1948 1986
Size: 21 90 39 38
Fragments arranged by size:
803 352 277 126 119 90 44 40
39 38 37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 1986
Size: 814 1172
EcoO109I rG'GnC_Cy
Cuts at: 0 198 1986
Size: 198 1788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 921 1986
Size: 921 1065
HaeII r_GCGC'y
Cuts at: 0 1965 1986
Size: 1965 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 253 255 285 353 385 884 916
Size: 223 30 2 30 68 32 499 32
Cuts at: 916 1734 1766 1941 1973 1986
Size: 818 32 175 32 13
Fragments arranged by size:
818 499 223 175 68 32 32 32
32 30 30 13 2
HincII GTy'rAC
Cuts at: 0 1086 1986
Size: 1086 900
KasI G'GCGC_C
Cuts at: 0 1961 1986
Size: 1961 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1245 1986
Size: 239 1006 741
Fragments arranged by size:
1006 741 239
MspA1I CmG'CkG
Cuts at: 0 40 296 318 867 1880 1986
Size: 40 256 22 549 1013 106
Fragments arranged by size:
1013 549 256 106 40 22
NaeI GCC'GGC
Cuts at: 0 1403 1966 1986
Size: 1403 563 20
Fragments arranged by size:
1403 563 20
NarI GG'CG_CC
Cuts at: 0 1962 1986
Size: 1962 24
NcoI C'CATG_G
Cuts at: 0 1718 1986
Size: 1718 268
NgoMIV G'CCGG_C
Cuts at: 0 1401 1964 1986
Size: 1401 563 22
Fragments arranged by size:
1401 563 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 1986
Size: 702 1284
PfoI T'CCnGG_A
Cuts at: 0 512 857 1986
Size: 512 345 1129
Fragments arranged by size:
1129 512 345
PpuMI rG'GwC_Cy
Cuts at: 0 198 1986
Size: 198 1788
PstI C_TGCA'G
Cuts at: 0 890 1853 1862 1986
Size: 890 963 9 124
Fragments arranged by size:
963 890 124 9
PvuII CAG'CTG
Cuts at: 0 40 867 1880 1986
Size: 40 827 1013 106
Fragments arranged by size:
1013 827 106 40
SacI G_AGCT'C
Cuts at: 0 241 253 992 1986
Size: 241 12 739 994
Fragments arranged by size:
994 739 241 12
SalI G'TCGA_C
Cuts at: 0 1084 1986
Size: 1084 902
SapI GCTCTTCn'nnn_
Cuts at: 0 1391 1986
Size: 1391 595
SbfI CC_TGCA'GG
Cuts at: 0 1862 1986
Size: 1862 124
SexAI A'CCwGG_T
Cuts at: 0 1604 1986
Size: 1604 382
SfcI C'TryA_G
Cuts at: 0 886 1184 1849 1858 1875 1986
Size: 886 298 665 9 17 111
Fragments arranged by size:
886 665 298 111 17 9
SfoI GGC'GCC
Cuts at: 0 1963 1986
Size: 1963 23
StyI C'CwwG_G
Cuts at: 0 1718 1986
Size: 1718 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 932 1986
Size: 932 1054
TatI w'GTAC_w
Cuts at: 0 611 1243 1986
Size: 611 632 743
Fragments arranged by size:
743 632 611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 555 1986
Size: 315 240 1431
Fragments arranged by size:
1431 315 240
XmnI GAAnn'nnTTC
Cuts at: 0 901 1199 1986
Size: 901 298 787
Fragments arranged by size:
901 787 298
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI AvaI BanI BanII
BbeI BbsI BcgI BclI BglII BlpI BmgBI BmrI
BplI BpmI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MspA1I NaeI NarI
NcoI NgoMIV PflMI PfoI PpuMI PstI PvuII SacI
SalI SapI SbfI SexAI SfcI SfoI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbvCI BciVI BfrBI BglI Bme1580I
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BseYI BsiEI
BsmBI BspEI BspMI BsrGI BssHII BstBI BstEII Bsu36I
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
MslI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI