Restriction map for
pFN21AB6189
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AccIII AclI Aor51HI
ApaI AscI Asp700I Asp718I AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HaeII HgaI
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 979 GACGTC GACGT^C
AcyI 979 GACGTC G[GA]^CG[CT]C
AfaI 7 GTAC GT^AC
AflII 495 CTTAAG C^TTAAG
AhdI 306 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1240 GTGCAC G^TGCAC
AlwNI 1919 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 633 CCTGAGG CC^T[ACGT]AGG
ApaLI 1240 GTGCAC G^TGCAC
AseI 484 ATTAAT AT^TAAT
AsnI 484 ATTAAT AT^TAAT
AspEI 306 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1447 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 737 GCATGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 495 CTTAAG C^TTAAG
BpmI 889 CTCCAG CTGGAG(16/14)
BsaHI 979 GACGTC G[GA]^CG[CT]C
BsgI 1063 GTGCAG GTGCAG(16/14)
BsmBI 981 CGTCTC CGTCTC(1/5)
BsmI 1473 GAATGC GAATGC(1/-1)
BspHI 1701 TCATGA T^CATGA
BspLU11I 1892 ACATGT A^CATGT
BstAPI 1918 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 173 TTCGAA TT^CGAA
Bsu36I 633 CCTGAGG CC^T[ACGT]AGG
DraII 724 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1418 CCATGG C^C[GA][CT]GG
Eam1105I 306 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 633 CCTGAGG CC^T[ACGT]AGG
EcoNI 237 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 724 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1948 GATATC GAT^ATC
FauI 944 CCCGC CCCGC(4/6)
Hin1I 979 GACGTC G[GA]^CG[CT]C
MfeI 520 CAATTG C^AATTG
MslI 869 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1589 CAGCTG C[AC]G^C[GT]G
MunI 520 CAATTG C^AATTG
NcoI 1418 CCATGG C^CATGG
NspV 173 TTCGAA TT^CGAA
PflFI 1447 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 724 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 484 ATTAAT AT^TAAT
PvuII 1589 CAGCTG CAG^CTG
RcaI 1701 TCATGA T^CATGA
RsaI 7 GTAC GT^AC
SfuI 173 TTCGAA TT^CGAA
SphI 737 GCATGC GCATG^C
Tsp45I 304 GTGAC ^GT[GC]AC
Tth111I 1447 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 27 CGCG CG^CG
29 CGCG
AflIII 1360 ACACGT A^C[GA][CT]GT
1892 ACATGT
AgeI 80 ACCGGT A^CCGGT
1532 ACCGGT
AvaI 1301 CTCGAG C^[CT]CG[GA]G
1676 CTCGAG
AvaII 472 GGTCC G^G[AT]CC
725 GGACC
BalI 1640 TGGCCA TGG^CCA
1934 TGGCCA
BsaWI 80 ACCGGT [AT]^CCGG[AT]
1532 ACCGGT
BseRI 293 CTCCTC GAGGAG(10/8)
847 GAGGAG
BsoBI 1301 CTCGAG C^[CT]CG[GA]G
1676 CTCGAG
BstUI 27 CGCG CG^CG
29 CGCG
BstYI 801 GGATCT [GA]^GATC[CT]
1389 GGATCT
CfoI 28 GCGC GCG^C
1380 GCGC
Cfr13I 472 GGTCC G^G[ACGT]CC
725 GGACC
DraI 144 TTTAAA TTT^AAA
1354 TTTAAA
EaeI 1640 TGGCCA [CT]^GGCC[GA]
1934 TGGCCA
EarI 49 GAAGAG CTCTTC(1/4)
831 GAAGAG
EcoT22I 619 ATGCAT ATGCA^T
971 ATGCAT
HhaI 28 GCGC GCG^C
1380 GCGC
HinP1I 28 GCGC G^CGC
1380 GCGC
HincII 586 GTTGAC GT[CT]^[GA]AC
639 GTCAAC
HindII 586 GTTGAC GT[CT]^[GA]AC
639 GTCAAC
HindIII 917 AAGCTT A^AGCTT
925 AAGCTT
Ksp632I 49 GAAGAG CTCTTC(1/4)
831 GAAGAG
MflI 801 GGATCT [GA]^GATC[CT]
1389 GGATCT
MluNI 1640 TGGCCA TGG^CCA
1934 TGGCCA
MscI 1640 TGGCCA TGG^CCA
1934 TGGCCA
MvnI 27 CGCG CG^CG
29 CGCG
NsiI 619 ATGCAT ATGCA^T
971 ATGCAT
PaeR7I 1301 CTCGAG C^TCGAG
1676 CTCGAG
PinAI 80 ACCGGT A^CCGGT
1532 ACCGGT
PleI 180 GAGTC GAGTC(4/5)
1304 GAGTC
PstI 37 CTGCAG CTGCA^G
1460 CTGCAG
Sau96I 472 GGTCC G^G[ACGT]CC
725 GGACC
SfcI 37 CTGCAG C^T[GA][CT]AG
1460 CTGCAG
XcmI 1311 CCATGCTGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1685 CCAAGATTCTTTTGG
XhoI 1301 CTCGAG C^TCGAG
1676 CTCGAG
XhoII 801 GGATCT [GA]^GATC[CT]
1389 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr10I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI Sau3AI ScrFI SfaNI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I