(Circular) (Six-base) MAPSORT of: pFN21AB6189.seq Check: 8584 from: 1 to: 6685 pFN21AB6189 6685 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2766 3061 2766 Size: 295 6390 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2988 4495 278 Size: 53 83 186 936 1452 1507 2468 Fragments arranged by size: 2468 1507 1452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4028 1937 Size: 2091 4594 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2795 4748 6132 6400 1632 Size: 542 621 1953 1384 268 1917 Fragments arranged by size: 1953 1917 1384 621 542 268 AflII C'TTAA_G Cuts at: 820 1017 2500 820 Size: 197 1483 5005 Fragments arranged by size: 5005 1483 197 AflIII A'CryG_T Cuts at: 1203 3365 3897 5584 6561 1203 Size: 2162 532 1687 977 1327 Fragments arranged by size: 2162 1687 1327 977 532 AgeI A'CCGG_T Cuts at: 2085 3537 5516 2085 Size: 1452 1979 3254 Fragments arranged by size: 3254 1979 1452 AhdI GACnn_n'nnGTC Cuts at: 2316 5414 2316 Size: 3098 3587 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6685 AlwNI CAG_nnn'CTG Cuts at: 3929 4428 6000 3929 Size: 499 1572 4614 Fragments arranged by size: 4614 1572 499 ApaLI G'TGCA_C Cuts at: 3245 4742 5898 3245 Size: 1497 1156 4032 Fragments arranged by size: 4032 1497 1156 ApoI r'AATT_y Cuts at: 1493 2122 2146 2681 2768 2858 3047 3994 4220 Size: 629 24 535 87 90 189 947 226 Cuts at: 4220 4372 6481 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 947 629 535 226 189 152 90 87 24 AseI AT'TA_AT Cuts at: 160 1032 2490 5239 160 Size: 872 1458 2749 1606 Fragments arranged by size: 2749 1606 1458 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 3306 3681 4010 1870 Size: 73 1363 375 329 4545 Fragments arranged by size: 4545 1363 375 329 73 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4006 5461 618 Size: 325 217 627 2219 1455 1842 Fragments arranged by size: 2219 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4004 1391 Size: 2613 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 2528 3304 3351 928 Size: 971 281 348 776 47 4262 Fragments arranged by size: 4262 971 776 348 281 47 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2731 2765 4876 4910 1903 Size: 34 794 34 2111 34 3678 Fragments arranged by size: 3678 2111 794 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 1548 Size: 6685 BfrBI ATG'CAT Cuts at: 2626 2978 2626 Size: 352 6333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5296 136 Size: 107 122 71 631 605 3624 1525 Fragments arranged by size: 3624 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6680 6680 Size: 6685 BlpI GC'TnA_GC Cuts at: 4102 4102 Size: 6685 Bme1580I G_kGCm'C Cuts at: 1790 3249 4746 5902 1790 Size: 1459 1497 1156 2573 Fragments arranged by size: 2573 1497 1459 1156 BmgBI CAC'GTC Cuts at: 1318 2698 3641 1318 Size: 1380 943 4362 Fragments arranged by size: 4362 1380 943 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2728 2734 4416 Size: 416 53 83 399 24 1311 6 1682 Cuts at: 4416 5374 442 Size: 958 1753 Fragments arranged by size: 1753 1682 1311 958 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2879 5345 1239 Size: 1640 2466 2579 Fragments arranged by size: 2579 2466 1640 Bpu10I CC'TnA_GC Cuts at: 2702 2702 Size: 6685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5675 5973 4810 Size: 865 298 5522 Fragments arranged by size: 5522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5348 882 Size: 617 3849 2219 Fragments arranged by size: 3849 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4476 493 Size: 711 3272 2702 Fragments arranged by size: 3272 2702 711 BsaBI GATnn'nnATC Cuts at: 6679 6679 Size: 6685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2985 4492 4874 5509 Size: 53 83 186 936 1452 1507 382 635 Cuts at: 5509 275 Size: 1451 Fragments arranged by size: 1507 1452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2085 3537 5118 5516 5790 5937 6348 2085 Size: 1452 1581 398 274 147 411 2422 Fragments arranged by size: 2422 1581 1452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 3542 3572 1178 Size: 30 165 30 1182 30 927 30 4291 Fragments arranged by size: 4291 1182 927 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2289 2867 1186 Size: 1103 578 5004 Fragments arranged by size: 5004 1103 578 BseYI C'CCAG_C Cuts at: 5888 6384 5888 Size: 496 6189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3089 1163 Size: 573 1353 4759 Fragments arranged by size: 4759 1353 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2703 3249 4746 4831 5902 2030 Size: 673 546 1497 85 1071 2813 Fragments arranged by size: 2813 1497 1071 673 546 85 BsmI GAATG_Cn' Cuts at: 3484 4196 4289 3484 Size: 712 93 5880 Fragments arranged by size: 5880 712 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2992 2992 Size: 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2703 3249 4004 4746 4831 5902 Size: 399 240 673 546 755 742 85 1071 Cuts at: 5902 1391 Size: 2174 Fragments arranged by size: 2174 1071 755 742 673 546 399 240 85 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 3706 4574 6304 3706 Size: 868 1730 4087 Fragments arranged by size: 4087 1730 868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 2766 3061 4040 844 Size: 1725 197 295 979 3489 Fragments arranged by size: 3489 1725 979 295 197 BsrBI CCG'CTC Cuts at: 1394 1823 4572 1394 Size: 429 2749 3507 Fragments arranged by size: 3507 2749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5180 5354 65 Size: 1183 3932 174 1396 Fragments arranged by size: 3932 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 3537 3729 5329 5516 1700 Size: 110 275 1452 192 1600 187 2869 Fragments arranged by size: 2869 1600 1452 275 192 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6685 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 4745 5757 4745 Size: 1012 5673 BstAPI GCAn_nnn'nTGC Cuts at: 794 3929 794 Size: 3135 3550 BstBI TT'CG_AA Cuts at: 2179 5489 2179 Size: 3310 3375 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstYI r'GATC_y Cuts at: 1972 2806 3394 4015 4382 4767 4784 6236 6322 Size: 834 588 621 367 385 17 1452 86 Cuts at: 6322 6334 6680 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 834 621 588 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2639 2639 Size: 6685 BtgI C'CryG_G Cuts at: 513 1065 2004 3423 513 Size: 552 939 1419 3775 Fragments arranged by size: 3775 1419 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 487 Size: 683 56 1508 4438 Fragments arranged by size: 4438 1508 683 56 BtsI GCAGTG_nn' Cuts at: 4201 4995 5015 4201 Size: 794 20 5871 Fragments arranged by size: 5871 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6650 DraI TTT'AAA Cuts at: 2151 3361 3989 2151 Size: 1210 628 4847 Fragments arranged by size: 4847 1210 628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5692 809 Size: 507 4376 1802 Fragments arranged by size: 4376 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3645 3939 4132 Size: 54 1425 50 275 18 1815 294 193 Cuts at: 4132 5021 6650 8 Size: 889 1629 43 Fragments arranged by size: 1815 1629 1425 889 294 275 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2831 4615 1353 Size: 696 782 1784 3423 Fragments arranged by size: 3423 1784 782 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5272 5646 5792 387 Size: 1064 138 3683 374 146 1280 Fragments arranged by size: 3683 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2795 2879 4748 5345 6132 6400 Size: 393 542 621 84 1869 597 787 268 Cuts at: 6400 1239 Size: 1524 Fragments arranged by size: 1869 1524 787 621 597 542 393 268 84 EcoNI CCTnn'n_nnAGG Cuts at: 2246 2246 Size: 6685 EcoO109I rG'GnC_Cy Cuts at: 2730 2730 Size: 6685 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 EcoRV GAT'ATC Cuts at: 3955 3955 Size: 6685 FspI TGC'GCA Cuts at: 5191 5191 Size: 6685 HaeII r_GCGC'y Cuts at: 1678 5832 1678 Size: 4154 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2050 2080 2082 2302 Size: 32 135 32 476 2 30 2 220 Cuts at: 2302 2334 2501 2533 2585 2617 3441 3473 5326 Size: 32 167 32 52 32 824 32 1853 Cuts at: 5326 5358 5400 5432 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1853 824 476 220 167 135 52 42 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2593 2646 4029 4275 669 Size: 988 281 655 53 1383 246 3079 Fragments arranged by size: 3079 1383 988 655 281 246 53 HindIII A'AGCT_T Cuts at: 748 2922 2930 748 Size: 2174 8 4503 Fragments arranged by size: 4503 2174 8 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 2525 4284 2525 Size: 1759 4926 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5799 5983 1305 Size: 4494 184 2007 Fragments arranged by size: 4494 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3647 3941 10 Size: 54 1425 343 1815 294 2754 Fragments arranged by size: 2754 1815 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2878 4643 5002 5161 518 Size: 648 1712 1765 359 159 2042 Fragments arranged by size: 2042 1765 1712 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3596 4101 4778 5926 6171 1606 Size: 1990 505 677 1148 245 2120 Fragments arranged by size: 2120 1990 1148 677 505 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3423 513 Size: 552 939 1419 3775 Fragments arranged by size: 3775 1419 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NsiI A_TGCA'T Cuts at: 2628 2980 2628 Size: 352 6333 NspI r_CATG'y Cuts at: 1132 1229 2630 2746 3901 4043 5588 1132 Size: 97 1401 116 1155 142 1545 2229 Fragments arranged by size: 2229 1545 1401 1155 142 116 97 PasI CC'CwG_GG Cuts at: 1348 3104 1348 Size: 1756 4929 PciI A'CATG_T Cuts at: 3897 5584 3897 Size: 1687 4998 PflMI CCAn_nnn'nTGG Cuts at: 1108 3982 1108 Size: 2874 3811 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2730 2730 Size: 6685 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 2823 4255 2823 Size: 1432 5253 PspXI vC'TCGA_Gb Cuts at: 3306 3306 Size: 6685 PstI C_TGCA'G Cuts at: 830 2046 3469 4037 830 Size: 1216 1423 568 3478 Fragments arranged by size: 3478 1423 1216 568 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 PvuII CAG'CTG Cuts at: 3596 3596 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SanDI GG'GwC_CC Cuts at: 2730 2730 Size: 6685 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SfcI C'TryA_G Cuts at: 826 1045 2042 3465 4033 5168 5849 6040 826 Size: 219 997 1423 568 1135 681 191 1471 Fragments arranged by size: 1471 1423 1135 997 681 568 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2500 3306 3681 4789 5690 5952 Size: 197 926 557 806 375 1108 901 262 Cuts at: 5952 820 Size: 1553 Fragments arranged by size: 1553 1108 926 901 806 557 375 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 2746 4043 1132 Size: 1614 1297 3774 Fragments arranged by size: 3774 1614 1297 SspI AAT'ATT Cuts at: 5 52 2173 3261 3652 3885 4609 5 Size: 47 2121 1088 391 233 724 2081 Fragments arranged by size: 2121 2081 1088 724 391 233 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2004 2385 3423 3942 4124 513 Size: 552 939 381 1038 519 182 3074 Fragments arranged by size: 3074 1038 939 552 519 381 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4712 4729 4882 5067 5512 325 Size: 1255 3132 17 153 185 445 1498 Fragments arranged by size: 3132 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4931 6643 96 Size: 274 80 33 51 1443 2954 1712 138 Fragments arranged by size: 2954 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2330 2362 3228 3260 3315 3347 6645 6677 2330 Size: 32 866 32 55 32 3298 32 2338 Fragments arranged by size: 3298 2338 866 55 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3455 1316 Size: 2139 4546 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3323 3697 2011 Size: 1312 374 4999 Fragments arranged by size: 4999 1312 374 XhoI C'TCGA_G Cuts at: 1943 3306 3681 1943 Size: 1363 375 4947 Fragments arranged by size: 4947 1363 375 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 4814 4814 Size: 6685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2986 4493 276 Size: 53 83 186 936 1452 1507 2468 Fragments arranged by size: 2468 1507 1452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI