Restriction map for
pFN21AB6189
(Linear) (Six-base) MAPSORT of: KIBB6189.seq Check: 7307 from: 1 to: 1980
KIBB6189.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 761 1056 1980
Size: 761 295 924
Fragments arranged by size:
924 761 295
AatII G_ACGT'C
Cuts at: 0 983 1980
Size: 983 997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 790 1980
Size: 169 621 1190
Fragments arranged by size:
1190 621 169
AflII C'TTAA_G
Cuts at: 0 495 1980
Size: 495 1485
AflIII A'CryG_T
Cuts at: 0 1360 1892 1980
Size: 1360 532 88
Fragments arranged by size:
1360 532 88
AgeI A'CCGG_T
Cuts at: 0 80 1532 1980
Size: 80 1452 448
Fragments arranged by size:
1452 448 80
AhdI GACnn_n'nnGTC
Cuts at: 0 311 1980
Size: 311 1669
AlwNI CAG_nnn'CTG
Cuts at: 0 1924 1980
Size: 1924 56
ApaLI G'TGCA_C
Cuts at: 0 1240 1980
Size: 1240 740
ApoI r'AATT_y
Cuts at: 0 117 141 676 763 853 1042 1980
Size: 117 24 535 87 90 189 938
Fragments arranged by size:
938 535 189 117 90 87 24
AseI AT'TA_AT
Cuts at: 0 485 1980
Size: 485 1495
AvaI C'yCGr_G
Cuts at: 0 1301 1676 1980
Size: 1301 375 304
Fragments arranged by size:
1301 375 304
BbsI GAAGACnn'nnnn_
Cuts at: 0 175 523 1299 1346 1980
Size: 175 348 776 47 634
Fragments arranged by size:
776 634 348 175 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 760 1980
Size: 726 34 1220
Fragments arranged by size:
1220 726 34
BfrBI ATG'CAT
Cuts at: 0 621 973 1980
Size: 621 352 1007
Fragments arranged by size:
1007 621 352
Bme1580I G_kGCm'C
Cuts at: 0 1244 1980
Size: 1244 736
BmgBI CAC'GTC
Cuts at: 0 693 1636 1980
Size: 693 943 344
Fragments arranged by size:
943 693 344
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 729 1980
Size: 723 6 1251
Fragments arranged by size:
1251 723 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1980
Size: 874 1106
Bpu10I CC'TnA_GC
Cuts at: 0 697 1980
Size: 697 1283
BsaHI Gr'CG_yC
Cuts at: 0 980 1980
Size: 980 1000
BsaWI w'CCGG_w
Cuts at: 0 80 1532 1980
Size: 80 1452 448
Fragments arranged by size:
1452 448 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 1537 1567 1980
Size: 580 30 927 30 413
Fragments arranged by size:
927 580 413 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 284 862 1980
Size: 284 578 1118
Fragments arranged by size:
1118 578 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1980
Size: 1084 896
BsiHKAI G_wGCw'C
Cuts at: 0 25 698 1244 1980
Size: 25 673 546 736
Fragments arranged by size:
736 673 546 25
BsmI GAATG_Cn'
Cuts at: 0 1479 1980
Size: 1479 501
BsmBI CGTCTCn'nnnn_
Cuts at: 0 987 1980
Size: 987 993
Bsp1286I G_dGCh'C
Cuts at: 0 25 698 1244 1980
Size: 25 673 546 736
Fragments arranged by size:
736 673 546 25
BspHI T'CATG_A
Cuts at: 0 1701 1980
Size: 1701 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 761 1056 1980
Size: 564 197 295 924
Fragments arranged by size:
924 564 295 197
BsrFI r'CCGG_y
Cuts at: 0 80 1532 1724 1980
Size: 80 1452 192 256
Fragments arranged by size:
1452 256 192 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1924 1980
Size: 1924 56
BstBI TT'CG_AA
Cuts at: 0 174 1980
Size: 174 1806
BstYI r'GATC_y
Cuts at: 0 801 1389 1980
Size: 801 588 591
Fragments arranged by size:
801 591 588
Bsu36I CC'TnA_GG
Cuts at: 0 634 1980
Size: 634 1346
BtgI C'CryG_G
Cuts at: 0 1418 1980
Size: 1418 562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 1980
Size: 729 1251
DraI TTT'AAA
Cuts at: 0 146 1356 1980
Size: 146 1210 624
Fragments arranged by size:
1210 624 146
EaeI y'GGCC_r
Cuts at: 0 1640 1934 1980
Size: 1640 294 46
Fragments arranged by size:
1640 294 46
EarI CTCTTCn'nnn_
Cuts at: 0 44 826 1980
Size: 44 782 1154
Fragments arranged by size:
1154 782 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 790 874 1980
Size: 169 621 84 1106
Fragments arranged by size:
1106 621 169 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 1980
Size: 241 1739
EcoO109I rG'GnC_Cy
Cuts at: 0 725 1980
Size: 725 1255
EcoRV GAT'ATC
Cuts at: 0 1950 1980
Size: 1950 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 45 75 77 297 329 496 528
Size: 43 2 30 2 220 32 167 32
Cuts at: 528 580 612 1436 1468 1980
Size: 52 32 824 32 512
Fragments arranged by size:
824 512 220 167 52 43 32 32
32 32 30 2 2
HincII GTy'rAC
Cuts at: 0 588 641 1980
Size: 588 53 1339
Fragments arranged by size:
1339 588 53
HindIII A'AGCT_T
Cuts at: 0 917 925 1980
Size: 917 8 1055
Fragments arranged by size:
1055 917 8
MfeI C'AATT_G
Cuts at: 0 520 1980
Size: 520 1460
MscI TGG'CCA
Cuts at: 0 1642 1936 1980
Size: 1642 294 44
Fragments arranged by size:
1642 294 44
MslI CAynn'nnrTG
Cuts at: 0 873 1980
Size: 873 1107
MspA1I CmG'CkG
Cuts at: 0 1591 1980
Size: 1591 389
NcoI C'CATG_G
Cuts at: 0 1418 1980
Size: 1418 562
NsiI A_TGCA'T
Cuts at: 0 623 975 1980
Size: 623 352 1005
Fragments arranged by size:
1005 623 352
NspI r_CATG'y
Cuts at: 0 625 741 1896 1980
Size: 625 116 1155 84
Fragments arranged by size:
1155 625 116 84
PasI CC'CwG_GG
Cuts at: 0 1099 1980
Size: 1099 881
PciI A'CATG_T
Cuts at: 0 1892 1980
Size: 1892 88
PpuMI rG'GwC_Cy
Cuts at: 0 725 1980
Size: 725 1255
PsiI TTA'TAA
Cuts at: 0 818 1980
Size: 818 1162
PspXI vC'TCGA_Gb
Cuts at: 0 1301 1980
Size: 1301 679
PstI C_TGCA'G
Cuts at: 0 41 1464 1980
Size: 41 1423 516
Fragments arranged by size:
1423 516 41
PvuII CAG'CTG
Cuts at: 0 1591 1980
Size: 1591 389
SanDI GG'GwC_CC
Cuts at: 0 725 1980
Size: 725 1255
SfcI C'TryA_G
Cuts at: 0 37 1460 1980
Size: 37 1423 520
Fragments arranged by size:
1423 520 37
SmlI C'TyrA_G
Cuts at: 0 495 1301 1676 1980
Size: 495 806 375 304
Fragments arranged by size:
806 495 375 304
SphI G_CATG'C
Cuts at: 0 741 1980
Size: 741 1239
SspI AAT'ATT
Cuts at: 0 168 1256 1647 1880 1980
Size: 168 1088 391 233 100
Fragments arranged by size:
1088 391 233 168 100
StyI C'CwwG_G
Cuts at: 0 380 1418 1937 1980
Size: 380 1038 519 43
Fragments arranged by size:
1038 519 380 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 1223 1255 1310 1342 1980
Size: 325 32 866 32 55 32 638
Fragments arranged by size:
866 638 325 55 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1450 1980
Size: 1450 530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1318 1692 1980
Size: 1318 374 288
Fragments arranged by size:
1318 374 288
XhoI C'TCGA_G
Cuts at: 0 1301 1676 1980
Size: 1301 375 304
Fragments arranged by size:
1301 375 304
ZraI GAC'GTC
Cuts at: 0 981 1980
Size: 981 999
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AvaI BbsI BcgI BfrBI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII HindIII
MfeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PpuMI PsiI PspXI PstI PvuII SanDI SfcI
SmlI SphI SspI StyI TstI Tth111I XcmI XhoI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AleI AlfI AloI ApaI
AscI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI BmtI BplI
BpuEI BsaI BsaAI BsaBI BseYI BsiEI BsiWI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoRI FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI TaqII TatI
XbaI XmaI XmnI