(Circular) (Six-base) MAPSORT of: pFN21AB6175.seq Check: 90 from: 1 to: 6664 pFN21AB6175 6664 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2505 3258 2505 Size: 753 5911 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 1937 Size: 2070 4594 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2431 2648 2732 3495 4727 6111 6379 1632 Size: 799 217 84 763 1232 1384 268 1917 Fragments arranged by size: 1917 1384 1232 799 763 268 217 84 AfeI AGC'GCT Cuts at: 2540 2981 2540 Size: 441 6223 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 3774 5563 6540 1203 Size: 2571 1789 977 1327 Fragments arranged by size: 2571 1789 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 2609 3777 3806 1166 Size: 1443 1168 29 4024 Fragments arranged by size: 4024 1443 1168 29 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2627 3745 1184 Size: 1443 1118 4103 Fragments arranged by size: 4103 1443 1118 AlwNI CAG_nnn'CTG Cuts at: 2497 2809 3711 3790 4407 5979 2497 Size: 312 902 79 617 1572 3182 Fragments arranged by size: 3182 1572 902 617 312 79 ApaI G_GGCC'C Cuts at: 2518 3347 3595 3800 3860 2518 Size: 829 248 205 60 5322 Fragments arranged by size: 5322 829 248 205 60 ApaLI G'TGCA_C Cuts at: 2424 2431 2754 4721 5877 2424 Size: 7 323 1967 1156 3211 Fragments arranged by size: 3211 1967 1156 323 7 ApoI r'AATT_y Cuts at: 1493 3973 4199 4351 6460 1493 Size: 2480 226 152 2109 1697 Fragments arranged by size: 2480 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2130 2936 3090 3989 1870 Size: 73 104 83 806 154 899 4545 Fragments arranged by size: 4545 899 806 154 104 83 73 BamHI G'GATC_C Cuts at: 2051 3994 4361 2051 Size: 1943 367 4354 Fragments arranged by size: 4354 1943 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 3270 3823 3985 5440 Size: 325 217 627 1337 146 553 162 1455 Cuts at: 5440 618 Size: 1842 Fragments arranged by size: 1842 1455 1337 627 553 325 217 162 146 BanII G_rGCy'C Cuts at: 1391 2518 2745 3297 3347 3561 3595 3800 3860 Size: 1127 227 552 50 214 34 205 60 Cuts at: 3860 3983 1391 Size: 123 4072 Fragments arranged by size: 4072 1127 552 227 214 205 123 60 50 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2219 3245 928 Size: 971 320 1026 4347 Fragments arranged by size: 4347 1026 971 320 BbvCI CC'TCA_GC Cuts at: 2182 2945 3399 2182 Size: 763 454 5447 Fragments arranged by size: 5447 763 454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4855 4889 1903 Size: 34 50 34 2834 34 3678 Fragments arranged by size: 3678 2834 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2513 3795 Size: 107 122 71 631 605 337 504 1282 Cuts at: 3795 3812 3939 5275 136 Size: 17 127 1336 1525 Fragments arranged by size: 1525 1336 1282 631 605 504 337 127 122 107 71 17 BglII A'GATC_T Cuts at: 6659 6659 Size: 6664 BlpI GC'TnA_GC Cuts at: 2525 4081 2525 Size: 1556 5108 Bme1580I G_kGCm'C Cuts at: 1790 2428 2435 2518 2758 3347 3595 3800 3860 Size: 638 7 83 240 589 248 205 60 Cuts at: 3860 3960 4725 5881 1790 Size: 100 765 1156 2573 Fragments arranged by size: 2573 1156 765 638 589 248 240 205 100 83 60 7 BmgBI CAC'GTC Cuts at: 1318 3409 3702 1318 Size: 2091 293 4280 Fragments arranged by size: 4280 2091 293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2153 2776 3313 Size: 416 53 83 399 24 736 623 537 Cuts at: 3313 3766 4395 5353 442 Size: 453 629 958 1753 Fragments arranged by size: 1753 958 736 629 623 537 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 3056 3484 3643 5324 1239 Size: 872 945 428 159 1681 2579 Fragments arranged by size: 2579 1681 945 872 428 159 Bpu10I CC'TnA_GC Cuts at: 2175 2182 2945 3399 2175 Size: 7 763 454 5440 Fragments arranged by size: 5440 763 454 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2519 4789 5654 5952 2519 Size: 2270 865 298 3231 Fragments arranged by size: 3231 2270 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5327 882 Size: 617 3828 2219 Fragments arranged by size: 3828 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3448 3775 4455 493 Size: 711 2244 327 680 2702 Fragments arranged by size: 2702 2244 711 680 327 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2774 2817 2888 3324 Size: 53 83 186 936 1241 43 71 436 Cuts at: 3324 3627 4471 4853 5488 275 Size: 303 844 382 635 1451 Fragments arranged by size: 1451 1241 936 844 635 436 382 303 186 83 71 53 43 BsaWI w'CCGG_w Cuts at: 5097 5495 5769 5916 6327 5097 Size: 398 274 147 411 5434 Fragments arranged by size: 5434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6439 Fragments arranged by size: 6439 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 2818 2833 2896 3305 1186 Size: 1474 158 15 63 409 4545 Fragments arranged by size: 4545 1474 409 158 63 15 BseYI C'CCAG_C Cuts at: 2152 2200 2237 2503 2614 2793 2976 2990 3887 Size: 48 37 266 111 179 183 14 897 Cuts at: 3887 3929 5867 6363 2152 Size: 42 1938 496 2453 Fragments arranged by size: 2453 1938 897 496 266 183 179 111 48 42 37 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2732 2915 3724 1163 Size: 573 996 183 809 4103 Fragments arranged by size: 4103 996 809 573 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 2643 2693 3116 3286 4114 Size: 275 186 343 299 50 423 170 828 Cuts at: 4114 4875 5903 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 828 761 423 343 299 275 186 170 50 BsiHKAI G_wGCw'C Cuts at: 2428 2435 2745 2758 2943 3297 3561 4725 4810 Size: 7 310 13 185 354 264 1164 85 Cuts at: 4810 5881 2428 Size: 1071 3211 Fragments arranged by size: 3211 1164 1071 354 310 264 185 85 13 7 BsmI GAATG_Cn' Cuts at: 4175 4268 4175 Size: 93 6571 BsmBI CGTCTCn'nnnn_ Cuts at: 3692 3692 Size: 6664 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2428 2435 2518 2745 2758 2943 3297 Size: 399 638 7 83 227 13 185 354 Cuts at: 3297 3347 3561 3595 3800 3860 3960 3983 4725 Size: 50 214 34 205 60 100 23 742 Cuts at: 4725 4810 5881 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 399 354 227 214 205 185 100 85 83 60 50 34 23 13 7 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 4553 6283 4553 Size: 1730 4934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2505 2730 3258 4019 844 Size: 1661 225 528 761 3489 Fragments arranged by size: 3489 1661 761 528 225 BsrBI CCG'CTC Cuts at: 1394 1823 2097 3350 3589 4551 1394 Size: 429 274 1253 239 962 3507 Fragments arranged by size: 3507 1253 962 429 274 239 BsrDI GCAATG_nn' Cuts at: 65 1248 2458 5159 5333 65 Size: 1183 1210 2701 174 1396 Fragments arranged by size: 2701 1396 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2114 3383 3440 3896 5308 5495 Size: 110 198 106 1269 57 456 1412 187 Cuts at: 5495 1700 Size: 2869 Fragments arranged by size: 2869 1412 1269 456 198 187 110 106 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6664 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 3554 4724 5736 3554 Size: 1170 1012 4482 Fragments arranged by size: 4482 1170 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 2809 3711 794 Size: 1292 723 902 3747 Fragments arranged by size: 3747 1292 902 723 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 3779 3808 3779 Size: 29 6635 BstYI r'GATC_y Cuts at: 1972 2051 3994 4361 4746 4763 6215 6301 6313 Size: 79 1943 367 385 17 1452 86 12 Cuts at: 6313 6659 1972 Size: 346 1977 Fragments arranged by size: 1977 1943 1452 385 367 346 86 79 17 12 Bsu36I CC'TnA_GG Cuts at: 3453 3453 Size: 6664 BtgI C'CryG_G Cuts at: 513 1065 2338 3048 3357 3567 3807 513 Size: 552 1273 710 309 210 240 3370 Fragments arranged by size: 3370 1273 710 552 309 240 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2201 487 Size: 683 56 975 4950 Fragments arranged by size: 4950 975 683 56 BtsI GCAGTG_nn' Cuts at: 2552 4180 4974 4994 2552 Size: 1628 794 20 4222 Fragments arranged by size: 4222 1628 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6629 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DraIII CAC_nnn'GTG Cuts at: 2086 3775 2086 Size: 1689 4975 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2341 2460 2690 Size: 54 1425 50 275 18 511 119 230 Cuts at: 2690 2763 2954 3113 3597 3781 3813 4111 5000 Size: 73 191 159 484 184 32 298 889 Cuts at: 5000 6629 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 511 484 298 275 230 191 184 159 119 73 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2341 2690 3113 4111 1537 Size: 275 529 349 423 998 4090 Fragments arranged by size: 4090 998 529 423 349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3180 3471 4594 1353 Size: 1827 291 1123 3423 Fragments arranged by size: 3423 1827 1123 291 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3370 3700 5251 5625 5771 387 Size: 1064 138 1781 330 1551 374 146 1280 Fragments arranged by size: 1781 1551 1280 1064 374 330 146 138 EcoICRI GAG'CTC Cuts at: 2743 3295 3559 2743 Size: 552 264 5848 Fragments arranged by size: 5848 552 264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2431 2648 2732 3056 3484 3495 Size: 393 479 320 217 84 324 428 11 Cuts at: 3495 3643 4727 5324 6111 6379 1239 Size: 148 1084 597 787 268 1524 Fragments arranged by size: 1524 1084 787 597 479 428 393 324 320 268 217 148 84 11 EcoNI CCTnn'n_nnAGG Cuts at: 3599 3599 Size: 6664 EcoO109I rG'GnC_Cy Cuts at: 2135 2514 3132 3343 3344 3395 3432 3592 3735 Size: 379 618 211 1 51 37 160 143 Cuts at: 3735 3796 3797 3857 3908 2135 Size: 61 1 60 51 4891 Fragments arranged by size: 4891 618 379 211 160 143 61 60 51 51 37 1 1 EcoRI G'AATT_C Cuts at: 3973 4351 3973 Size: 378 6286 FspI TGC'GCA Cuts at: 2603 3266 5170 2603 Size: 663 1904 4097 Fragments arranged by size: 4097 1904 663 FspAI rTGC'GCAy Cuts at: 2603 2603 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 2542 2826 2983 3109 3250 3316 5811 1678 Size: 864 284 157 126 141 66 2495 2531 Fragments arranged by size: 2531 2495 864 284 157 141 126 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 5305 5337 5379 Size: 32 135 32 1325 32 2376 32 42 Cuts at: 5379 5411 1373 Size: 32 2626 Fragments arranged by size: 2626 2376 1325 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2866 4008 4254 669 Size: 988 281 928 1142 246 3079 Fragments arranged by size: 3079 1142 988 928 281 246 HindIII A'AGCT_T Cuts at: 748 2660 748 Size: 1912 4752 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3492 3513 5778 5962 1305 Size: 2187 21 2265 184 2007 Fragments arranged by size: 2265 2187 2007 184 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 3783 10 Size: 54 1425 343 933 1018 2891 Fragments arranged by size: 2891 1425 1018 933 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 3047 3227 3777 3806 4622 4981 Size: 648 1443 438 180 550 29 816 359 Cuts at: 4981 5140 518 Size: 159 2042 Fragments arranged by size: 2042 1443 816 648 550 438 359 180 159 29 MspA1I CmG'CkG Cuts at: 1606 2204 2737 2929 3359 3708 4080 4757 5905 Size: 598 533 192 430 349 372 677 1148 Cuts at: 5905 6150 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 598 533 430 372 349 245 192 NaeI GCC'GGC Cuts at: 2010 2116 3898 2010 Size: 106 1782 4776 Fragments arranged by size: 4776 1782 106 NcoI C'CATG_G Cuts at: 513 1065 3048 513 Size: 552 1983 4129 Fragments arranged by size: 4129 1983 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NgoMIV G'CCGG_C Cuts at: 2008 2114 3896 2008 Size: 106 1782 4776 Fragments arranged by size: 4776 1782 106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NruI TCG'CGA Cuts at: 3417 3417 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 2245 4022 5567 1132 Size: 97 1016 1777 1545 2229 Fragments arranged by size: 2229 1777 1545 1016 97 PasI CC'CwG_GG Cuts at: 1348 2269 1348 Size: 921 5743 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2588 2788 3853 1108 Size: 1480 200 1065 3919 Fragments arranged by size: 3919 1480 1065 200 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PmlI CAC'GTG Cuts at: 3775 3775 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 3132 3432 3735 2135 Size: 997 300 303 5064 Fragments arranged by size: 5064 997 303 300 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2514 3343 3591 3796 3856 2514 Size: 829 248 205 60 5322 Fragments arranged by size: 5322 829 248 205 60 PstI C_TGCA'G Cuts at: 830 2559 2736 3522 3795 4016 830 Size: 1729 177 786 273 221 3478 Fragments arranged by size: 3478 1729 786 273 221 177 PvuI CG_AT'CG Cuts at: 2001 2643 2001 Size: 642 6022 SacI G_AGCT'C Cuts at: 2745 3297 3561 2745 Size: 552 264 5848 Fragments arranged by size: 5848 552 264 SacII CC_GC'GG Cuts at: 3360 3360 Size: 6664 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SanDI GG'GwC_CC Cuts at: 2135 3132 2135 Size: 997 5667 SbfI CC_TGCA'GG Cuts at: 3795 4016 3795 Size: 221 6443 ScaI AGT'ACT Cuts at: 2786 4912 6624 2786 Size: 2126 1712 2826 Fragments arranged by size: 2826 2126 1712 SfcI C'TryA_G Cuts at: 826 1045 2555 2732 2983 3316 3518 3791 4012 Size: 219 1510 177 251 333 202 273 221 Cuts at: 4012 5147 5828 6019 826 Size: 1135 681 191 1471 Fragments arranged by size: 1510 1471 1135 681 333 273 251 221 219 202 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 2132 3092 3991 1872 Size: 260 960 899 4545 Fragments arranged by size: 4545 960 899 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2534 4768 5669 5931 820 Size: 197 926 591 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 591 262 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 2245 4022 1132 Size: 1113 1777 3774 Fragments arranged by size: 3774 1777 1113 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 2180 3617 1517 Size: 663 1437 4564 Fragments arranged by size: 4564 1437 663 StyI C'CwwG_G Cuts at: 513 1065 3048 4103 513 Size: 552 1983 1055 3074 Fragments arranged by size: 3074 1983 1055 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2452 3096 3863 4691 4708 4861 5046 Size: 1255 872 644 767 828 17 153 185 Cuts at: 5046 5491 325 Size: 445 1498 Fragments arranged by size: 1498 1255 872 828 767 644 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4910 6622 Size: 274 80 33 51 1443 807 2126 1712 Cuts at: 6622 96 Size: 138 Fragments arranged by size: 2126 1712 1443 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3679 3711 6624 6656 3679 Size: 32 2913 32 3687 Fragments arranged by size: 3687 2913 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3808 3808 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 2130 3090 3989 1870 Size: 260 960 899 4545 Fragments arranged by size: 4545 960 899 260 XmnI GAAnn'nnTTC Cuts at: 4793 4793 Size: 6664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI KasI NarI NsiI PacI PfoI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI