(Circular) (Six-base) MAPSORT of: pFN21AB6171.seq Check: 6037 from: 1 to: 6652 pFN21AB6171 6652 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3843 3843 Size: 6652 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4462 278 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3995 1937 Size: 2058 4594 Acc65I G'GTAC_C Cuts at: 3973 3973 Size: 6652 AclI AA'CG_TT Cuts at: 1563 4779 5152 1563 Size: 3216 373 3063 Fragments arranged by size: 3216 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2644 4715 6099 6367 1632 Size: 767 245 2071 1384 268 1917 Fragments arranged by size: 2071 1917 1384 767 268 245 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6455 AflIII A'CryG_T Cuts at: 1203 5551 6528 1203 Size: 4348 977 1327 Fragments arranged by size: 4348 1327 977 AgeI A'CCGG_T Cuts at: 5483 5483 Size: 6652 AhdI GACnn_n'nnGTC Cuts at: 5381 5381 Size: 6652 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6652 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3530 3562 3530 Size: 32 6620 AlwNI CAG_nnn'CTG Cuts at: 3940 4395 5967 3940 Size: 455 1572 4625 Fragments arranged by size: 4625 1572 455 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 6652 ApaLI G'TGCA_C Cuts at: 3118 4709 5865 3118 Size: 1591 1156 3905 Fragments arranged by size: 3905 1591 1156 ApoI r'AATT_y Cuts at: 1493 2815 3961 4187 4339 6448 1493 Size: 1322 1146 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 1146 226 152 AseI AT'TA_AT Cuts at: 160 1032 5206 160 Size: 872 4174 1606 Fragments arranged by size: 4174 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 AvaI C'yCGr_G Cuts at: 1870 1943 2665 3977 1870 Size: 73 722 1312 4545 Fragments arranged by size: 4545 1312 722 73 AvrII C'CTAG_G Cuts at: 3847 3847 Size: 6652 BamHI G'GATC_C Cuts at: 3982 4349 3982 Size: 367 6285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2068 2725 3973 5428 Size: 325 217 627 221 60 657 1248 1455 Cuts at: 5428 618 Size: 1842 Fragments arranged by size: 1842 1455 1248 657 627 325 221 217 60 BanII G_rGCy'C Cuts at: 1391 2063 3108 3563 3951 3971 1391 Size: 672 1045 455 388 20 4072 Fragments arranged by size: 4072 1045 672 455 388 20 BbeI G_GCGC'C Cuts at: 2072 2072 Size: 6652 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 3652 928 Size: 971 322 1431 3928 Fragments arranged by size: 3928 1431 971 322 BbvCI CC'TCA_GC Cuts at: 2763 2763 Size: 6652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4843 4877 1903 Size: 34 2906 34 3678 Fragments arranged by size: 3678 2906 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3024 4544 5754 3024 Size: 1520 1210 3922 Fragments arranged by size: 3922 1520 1210 BclI T'GATC_A Cuts at: 1548 2494 2991 1548 Size: 946 497 5209 Fragments arranged by size: 5209 946 497 BfrBI ATG'CAT Cuts at: 2842 2842 Size: 6652 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3609 3771 5263 Size: 107 122 71 631 605 1937 162 1492 Cuts at: 5263 136 Size: 1525 Fragments arranged by size: 1937 1525 1492 631 605 162 122 107 71 BglII A'GATC_T Cuts at: 3180 6647 3180 Size: 3467 3185 BlpI GC'TnA_GC Cuts at: 4069 4069 Size: 6652 Bme1580I G_kGCm'C Cuts at: 1790 2063 3122 3235 3864 4713 5869 1790 Size: 273 1059 113 629 849 1156 2573 Fragments arranged by size: 2573 1156 1059 849 629 273 113 BmgBI CAC'GTC Cuts at: 1318 3139 1318 Size: 1821 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3229 4383 5341 Size: 416 53 83 399 24 1812 1154 958 Cuts at: 5341 442 Size: 1753 Fragments arranged by size: 1812 1753 1154 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2753 3000 3161 3825 5312 1239 Size: 1514 247 161 664 1487 2579 Fragments arranged by size: 2579 1514 1487 664 247 161 Bpu10I CC'TnA_GC Cuts at: 2763 2985 2763 Size: 222 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4777 5642 5940 4777 Size: 865 298 5489 Fragments arranged by size: 5489 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 2873 5315 882 Size: 617 858 516 2442 2219 Fragments arranged by size: 2442 2219 858 617 516 BsaAI yAC'GTr Cuts at: 493 1204 4443 493 Size: 711 3239 2702 Fragments arranged by size: 3239 2702 711 BsaBI GATnn'nnATC Cuts at: 3012 6646 3012 Size: 3634 3018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 4459 4841 5476 Size: 53 83 186 936 536 2390 382 635 Cuts at: 5476 275 Size: 1451 Fragments arranged by size: 2390 1451 936 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 2472 5085 5483 5757 5904 6315 2271 Size: 201 2613 398 274 147 411 2608 Fragments arranged by size: 2613 2608 411 398 274 201 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2973 3003 3425 3455 1178 Size: 30 165 30 1570 30 422 30 4375 Fragments arranged by size: 4375 1570 422 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2213 2780 1186 Size: 970 57 567 5058 Fragments arranged by size: 5058 970 567 57 BseYI C'CCAG_C Cuts at: 3343 3562 3607 5855 6351 3343 Size: 219 45 2248 496 3644 Fragments arranged by size: 3644 2248 496 219 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6079 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2152 4102 4863 5891 1540 Size: 275 186 151 1950 761 1028 2301 Fragments arranged by size: 2301 1950 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2627 3122 3951 4713 4798 5869 2627 Size: 495 829 762 85 1071 3410 Fragments arranged by size: 3410 1071 829 762 495 85 BsmI GAATG_Cn' Cuts at: 3078 4163 4256 3078 Size: 1085 93 5474 Fragments arranged by size: 5474 1085 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2446 2446 Size: 6652 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2627 3108 3122 3235 3563 3864 Size: 399 273 564 481 14 113 328 301 Cuts at: 3864 3951 3971 4713 4798 5869 1391 Size: 87 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 481 399 328 301 273 113 87 85 20 14 BspEI T'CCGG_A Cuts at: 2271 6315 2271 Size: 4044 2608 BspHI T'CATG_A Cuts at: 4541 6271 4541 Size: 1730 4922 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 3843 4007 844 Size: 1945 1054 164 3489 Fragments arranged by size: 3489 1945 1054 164 BsrBI CCG'CTC Cuts at: 1394 1823 4539 1394 Size: 429 2716 3507 Fragments arranged by size: 3507 2716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3060 3684 5147 5321 65 Size: 1183 1812 624 1463 174 1396 Fragments arranged by size: 1812 1463 1396 1183 624 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3471 5296 5483 1700 Size: 110 1661 1825 187 2869 Fragments arranged by size: 2869 1825 1661 187 110 BsrGI T'GTAC_A Cuts at: 96 2934 96 Size: 2838 3814 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6652 BssSI C'ACGA_G Cuts at: 2966 4712 5724 2966 Size: 1746 1012 3894 Fragments arranged by size: 3894 1746 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6652 BstBI TT'CG_AA Cuts at: 5456 5456 Size: 6652 BstEII G'GTnAC_C Cuts at: 1184 2776 3058 1184 Size: 1592 282 4778 Fragments arranged by size: 4778 1592 282 BstXI CCAn_nnnn'nTGG Cuts at: 2605 3406 2605 Size: 801 5851 BstYI r'GATC_y Cuts at: 1972 3180 3545 3982 4349 4734 4751 6203 6289 Size: 1208 365 437 367 385 17 1452 86 Cuts at: 6289 6301 6647 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1208 437 385 367 365 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2064 Size: 6652 BtgI C'CryG_G Cuts at: 513 1065 2004 2119 2146 2193 2898 513 Size: 552 939 115 27 47 705 4267 Fragments arranged by size: 4267 939 705 552 115 47 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5913 Fragments arranged by size: 5913 683 56 BtsI GCAGTG_nn' Cuts at: 2219 4168 4962 4982 2219 Size: 1949 794 20 3889 Fragments arranged by size: 3889 1949 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6652 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3195 3230 3580 3615 529 Size: 35 2631 35 350 35 3566 Fragments arranged by size: 3566 2631 350 35 35 35 DraI TTT'AAA Cuts at: 3382 3956 3382 Size: 574 6078 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5659 809 Size: 507 4343 1802 Fragments arranged by size: 4343 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2149 3416 3507 Size: 54 1425 50 275 18 319 1267 91 Cuts at: 3507 3601 4099 4988 6617 8 Size: 94 498 889 1629 43 Fragments arranged by size: 1629 1425 1267 889 498 319 275 94 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2149 4099 1537 Size: 275 337 1950 4090 Fragments arranged by size: 4090 1950 337 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 3333 4582 1353 Size: 1587 393 1249 3423 Fragments arranged by size: 3423 1587 1249 393 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2438 5239 5613 5759 387 Size: 1064 138 849 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 849 374 146 138 EcoICRI GAG'CTC Cuts at: 3949 3949 Size: 6652 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2644 2753 3000 3161 3825 4715 Size: 393 767 245 109 247 161 664 890 Cuts at: 4715 5312 6099 6367 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 890 787 767 664 597 393 268 247 245 161 109 EcoO109I rG'GnC_Cy Cuts at: 2060 2374 3906 2060 Size: 314 1532 4806 Fragments arranged by size: 4806 1532 314 EcoRI G'AATT_C Cuts at: 3961 4339 3961 Size: 378 6274 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3520 3520 Size: 6652 FspI TGC'GCA Cuts at: 5158 5158 Size: 6652 HaeII r_GCGC'y Cuts at: 1678 2072 2416 5799 1678 Size: 394 344 3383 2531 Fragments arranged by size: 3383 2531 394 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 2047 2079 2411 Size: 32 135 32 435 32 8 32 332 Cuts at: 2411 2443 2479 2511 2783 2815 2983 3015 3315 Size: 32 36 32 272 32 168 32 300 Cuts at: 3315 3347 3662 3694 5293 5325 5367 5399 1373 Size: 32 315 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 435 332 315 300 272 168 135 42 36 32 32 32 32 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 3996 4242 669 Size: 988 281 2058 246 3079 Fragments arranged by size: 3079 2058 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6652 HpaI GTT'AAC Cuts at: 4242 4242 Size: 6652 KasI G'GCGC_C Cuts at: 2068 2068 Size: 6652 KpnI G_GTAC'C Cuts at: 3977 3977 Size: 6652 MfeI C'AATT_G Cuts at: 4251 4251 Size: 6652 MluI A'CGCG_T Cuts at: 6528 6528 Size: 6652 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 2664 3615 3939 5766 5950 1305 Size: 1336 23 951 324 1827 184 2007 Fragments arranged by size: 2007 1827 1336 951 324 184 23 MscI TGG'CCA Cuts at: 10 64 1489 1832 3418 3509 3603 10 Size: 54 1425 343 1586 91 94 3059 Fragments arranged by size: 3059 1586 1425 343 94 91 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2897 4610 4969 5128 518 Size: 648 972 759 1713 359 159 2042 Fragments arranged by size: 2042 1713 972 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2482 3607 4068 4745 5893 6138 1606 Size: 542 334 1125 461 677 1148 245 2120 Fragments arranged by size: 2120 1148 1125 677 542 461 334 245 NaeI GCC'GGC Cuts at: 3473 3473 Size: 6652 NarI GG'CG_CC Cuts at: 2069 2069 Size: 6652 NcoI C'CATG_G Cuts at: 513 1065 2004 2119 2898 513 Size: 552 939 115 779 4267 Fragments arranged by size: 4267 939 779 552 115 NdeI CA'TA_TG Cuts at: 387 387 Size: 6652 NgoMIV G'CCGG_C Cuts at: 3471 3471 Size: 6652 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6652 NotI GC'GGCC_GC Cuts at: 4099 4099 Size: 6652 NsiI A_TGCA'T Cuts at: 2844 2844 Size: 6652 NspI r_CATG'y Cuts at: 1132 1229 4010 5555 1132 Size: 97 2781 1545 2229 Fragments arranged by size: 2781 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6652 PciI A'CATG_T Cuts at: 5551 5551 Size: 6652 PflMI CCAn_nnn'nTGG Cuts at: 1108 3717 3940 1108 Size: 2609 223 3820 Fragments arranged by size: 3820 2609 223 PfoI T'CCnGG_A Cuts at: 2405 2429 3333 2405 Size: 24 904 5724 Fragments arranged by size: 5724 904 24 PmeI GTTT'AAAC Cuts at: 3956 3956 Size: 6652 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2426 2458 4721 4753 6234 6266 2426 Size: 32 2263 32 1481 32 2812 Fragments arranged by size: 2812 2263 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6652 PsiI TTA'TAA Cuts at: 4222 4222 Size: 6652 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 6652 PstI C_TGCA'G Cuts at: 830 2293 3262 3377 4004 830 Size: 1463 969 115 627 3478 Fragments arranged by size: 3478 1463 969 627 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6652 PvuII CAG'CTG Cuts at: 3607 3607 Size: 6652 SacI G_AGCT'C Cuts at: 3951 3951 Size: 6652 SacII CC_GC'GG Cuts at: 2149 2149 Size: 6652 SalI G'TCGA_C Cuts at: 1936 3994 1936 Size: 2058 4594 SapI GCTCTTCn'nnn_ Cuts at: 3333 3333 Size: 6652 SbfI CC_TGCA'GG Cuts at: 4004 4004 Size: 6652 ScaI AGT'ACT Cuts at: 4900 6612 4900 Size: 1712 4940 SfcI C'TryA_G Cuts at: 826 1045 2289 3066 3109 3258 3373 4000 5135 Size: 219 1244 777 43 149 115 627 1135 Cuts at: 5135 5816 6007 826 Size: 681 191 1471 Fragments arranged by size: 1471 1244 1135 777 681 627 219 191 149 115 43 SfiI GGCCn_nnn'nGGCC Cuts at: 3609 3609 Size: 6652 SfoI GGC'GCC Cuts at: 2070 2070 Size: 6652 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6652 SmaI CCC'GGG Cuts at: 1872 3979 1872 Size: 2107 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4756 5657 5919 820 Size: 197 926 2813 901 262 1553 Fragments arranged by size: 2813 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4443 493 Size: 3950 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6652 SphI G_CATG'C Cuts at: 1132 4010 1132 Size: 2878 3774 SspI AAT'ATT Cuts at: 5 52 4576 5 Size: 47 4524 2081 Fragments arranged by size: 4524 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6652 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2898 3466 3711 3847 4091 Size: 552 939 115 779 568 245 136 244 Cuts at: 4091 513 Size: 3074 Fragments arranged by size: 3074 939 779 568 552 245 244 136 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 3216 4679 4696 4849 5034 5479 Size: 1255 552 1084 1463 17 153 185 445 Cuts at: 5479 325 Size: 1498 Fragments arranged by size: 1498 1463 1255 1084 552 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2934 3237 Size: 274 80 33 51 1443 345 612 303 Cuts at: 3237 4898 6610 96 Size: 1661 1712 138 Fragments arranged by size: 1712 1661 1443 612 345 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2973 3005 3843 3875 6612 6644 2579 Size: 32 362 32 838 32 2737 32 2587 Fragments arranged by size: 2737 2587 838 362 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3085 1316 Size: 1769 4883 XbaI T'CTAG_A Cuts at: 3988 3988 Size: 6652 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2840 2605 Size: 235 6417 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6652 XmaI C'CCGG_G Cuts at: 1870 3977 1870 Size: 2107 4545 XmnI GAAnn'nnTTC Cuts at: 4781 4781 Size: 6652 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4460 276 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SrfI SwaI