Restriction map for
pFN21AB6171
(Linear) (Six-base) MAPSORT of: KIBB6171.seq Check: 2414 from: 1 to: 1947
KIBB6171.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1838 1947
Size: 1838 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 639 1947
Size: 394 245 1308
Fragments arranged by size:
1308 394 245
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 1947
Size: 1525 32 390
Fragments arranged by size:
1525 390 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1935 1947
Size: 1935 12
ApaI G_GGCC'C
Cuts at: 0 58 1947
Size: 58 1889
ApaLI G'TGCA_C
Cuts at: 0 1113 1947
Size: 1113 834
ApoI r'AATT_y
Cuts at: 0 810 1947
Size: 810 1137
AvaI C'yCGr_G
Cuts at: 0 660 1947
Size: 660 1287
AvrII C'CTAG_G
Cuts at: 0 1842 1947
Size: 1842 105
BanI G'GyrC_C
Cuts at: 0 3 63 720 1947
Size: 3 60 657 1227
Fragments arranged by size:
1227 657 60 3
BanII G_rGCy'C
Cuts at: 0 58 1103 1558 1946 1947
Size: 58 1045 455 388 1
Fragments arranged by size:
1045 455 388 58 1
BbeI G_GCGC'C
Cuts at: 0 67 1947
Size: 67 1880
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 1647 1947
Size: 216 1431 300
Fragments arranged by size:
1431 300 216
BbvCI CC'TCA_GC
Cuts at: 0 758 1947
Size: 758 1189
BciVI GTATCCnnnnn_n'
Cuts at: 0 1019 1947
Size: 1019 928
BclI T'GATC_A
Cuts at: 0 489 986 1947
Size: 489 497 961
Fragments arranged by size:
961 497 489
BfrBI ATG'CAT
Cuts at: 0 837 1947
Size: 837 1110
BglI GCCn_nnn'nGGC
Cuts at: 0 1604 1766 1947
Size: 1604 162 181
Fragments arranged by size:
1604 181 162
BglII A'GATC_T
Cuts at: 0 1175 1947
Size: 1175 772
Bme1580I G_kGCm'C
Cuts at: 0 58 1117 1230 1859 1947
Size: 58 1059 113 629 88
Fragments arranged by size:
1059 629 113 88 58
BmgBI CAC'GTC
Cuts at: 0 1134 1947
Size: 1134 813
BmrI ACTGGGnnnn_n'
Cuts at: 0 1224 1947
Size: 1224 723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 995 1156 1820 1947
Size: 748 247 161 664 127
Fragments arranged by size:
748 664 247 161 127
Bpu10I CC'TnA_GC
Cuts at: 0 758 980 1947
Size: 758 222 967
Fragments arranged by size:
967 758 222
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 868 1947
Size: 352 516 1079
Fragments arranged by size:
1079 516 352
BsaBI GATnn'nnATC
Cuts at: 0 1007 1947
Size: 1007 940
BsaHI Gr'CG_yC
Cuts at: 0 64 1947
Size: 64 1883
BsaWI w'CCGG_w
Cuts at: 0 266 467 1947
Size: 266 201 1480
Fragments arranged by size:
1480 266 201
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 968 998 1420 1450 1947
Size: 968 30 422 30 497
Fragments arranged by size:
968 497 422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 208 775 1947
Size: 151 57 567 1172
Fragments arranged by size:
1172 567 151 57
BseYI C'CCAG_C
Cuts at: 0 1338 1557 1602 1947
Size: 1338 219 45 345
Fragments arranged by size:
1338 345 219 45
BsiEI CG_ry'CG
Cuts at: 0 147 1947
Size: 147 1800
BsiHKAI G_wGCw'C
Cuts at: 0 622 1117 1946 1947
Size: 622 495 829 1
Fragments arranged by size:
829 622 495 1
BsmI GAATG_Cn'
Cuts at: 0 1073 1947
Size: 1073 874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 441 1947
Size: 441 1506
Bsp1286I G_dGCh'C
Cuts at: 0 58 622 1103 1117 1230 1558 1859 1946
Size: 58 564 481 14 113 328 301 87
Cuts at: 1946 1947
Size: 1
Fragments arranged by size:
564 481 328 301 113 87 58 14
1
BspEI T'CCGG_A
Cuts at: 0 266 1947
Size: 266 1681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 784 1838 1947
Size: 784 1054 109
Fragments arranged by size:
1054 784 109
BsrDI GCAATG_nn'
Cuts at: 0 1055 1679 1947
Size: 1055 624 268
Fragments arranged by size:
1055 624 268
BsrFI r'CCGG_y
Cuts at: 0 1466 1947
Size: 1466 481
BsrGI T'GTAC_A
Cuts at: 0 929 1947
Size: 929 1018
BssSI C'ACGA_G
Cuts at: 0 961 1947
Size: 961 986
BstEII G'GTnAC_C
Cuts at: 0 771 1053 1947
Size: 771 282 894
Fragments arranged by size:
894 771 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 600 1401 1947
Size: 600 801 546
Fragments arranged by size:
801 600 546
BstYI r'GATC_y
Cuts at: 0 1175 1540 1947
Size: 1175 365 407
Fragments arranged by size:
1175 407 365
Bsu36I CC'TnA_GG
Cuts at: 0 59 1947
Size: 59 1888
BtgI C'CryG_G
Cuts at: 0 114 141 188 893 1947
Size: 114 27 47 705 1054
Fragments arranged by size:
1054 705 114 47 27
BtsI GCAGTG_nn'
Cuts at: 0 214 1947
Size: 214 1733
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1190 1225 1575 1610 1947
Size: 1190 35 350 35 337
Fragments arranged by size:
1190 350 337 35 35
DraI TTT'AAA
Cuts at: 0 1377 1947
Size: 1377 570
EaeI y'GGCC_r
Cuts at: 0 144 1411 1502 1596 1947
Size: 144 1267 91 94 351
Fragments arranged by size:
1267 351 144 94 91
EagI C'GGCC_G
Cuts at: 0 144 1947
Size: 144 1803
EarI CTCTTCn'nnn_
Cuts at: 0 935 1328 1947
Size: 935 393 619
Fragments arranged by size:
935 619 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 433 1947
Size: 433 1514
EcoICRI GAG'CTC
Cuts at: 0 1944 1947
Size: 1944 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 639 748 995 1156 1820 1947
Size: 394 245 109 247 161 664 127
Fragments arranged by size:
664 394 247 245 161 127 109
EcoO109I rG'GnC_Cy
Cuts at: 0 55 369 1901 1947
Size: 55 314 1532 46
Fragments arranged by size:
1532 314 55 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1515 1947
Size: 1515 432
HaeII r_GCGC'y
Cuts at: 0 67 411 1947
Size: 67 344 1536
Fragments arranged by size:
1536 344 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 42 74 406 438 474 506
Size: 2 32 8 32 332 32 36 32
Cuts at: 506 778 810 978 1010 1310 1342 1657 1689
Size: 272 32 168 32 300 32 315 32
Cuts at: 1689 1947
Size: 258
Fragments arranged by size:
332 315 300 272 258 168 36 32
32 32 32 32 32 32 32 8
2
KasI G'GCGC_C
Cuts at: 0 63 1947
Size: 63 1884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 659 1610 1934 1947
Size: 636 23 951 324 13
Fragments arranged by size:
951 636 324 23 13
MscI TGG'CCA
Cuts at: 0 1413 1504 1598 1947
Size: 1413 91 94 349
Fragments arranged by size:
1413 349 94 91
MslI CAynn'nnrTG
Cuts at: 0 133 892 1947
Size: 133 759 1055
Fragments arranged by size:
1055 759 133
MspA1I CmG'CkG
Cuts at: 0 143 477 1602 1947
Size: 143 334 1125 345
Fragments arranged by size:
1125 345 334 143
NaeI GCC'GGC
Cuts at: 0 1468 1947
Size: 1468 479
NarI GG'CG_CC
Cuts at: 0 64 1947
Size: 64 1883
NcoI C'CATG_G
Cuts at: 0 114 893 1947
Size: 114 779 1054
Fragments arranged by size:
1054 779 114
NgoMIV G'CCGG_C
Cuts at: 0 1466 1947
Size: 1466 481
NsiI A_TGCA'T
Cuts at: 0 839 1947
Size: 839 1108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1712 1935 1947
Size: 1712 223 12
Fragments arranged by size:
1712 223 12
PfoI T'CCnGG_A
Cuts at: 0 400 424 1328 1947
Size: 400 24 904 619
Fragments arranged by size:
904 619 400 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 1947
Size: 421 32 1494
Fragments arranged by size:
1494 421 32
PspOMI G'GGCC_C
Cuts at: 0 54 1947
Size: 54 1893
PstI C_TGCA'G
Cuts at: 0 288 1257 1372 1947
Size: 288 969 115 575
Fragments arranged by size:
969 575 288 115
PvuII CAG'CTG
Cuts at: 0 1602 1947
Size: 1602 345
SacI G_AGCT'C
Cuts at: 0 1946 1947
Size: 1946 1
SacII CC_GC'GG
Cuts at: 0 144 1947
Size: 144 1803
SapI GCTCTTCn'nnn_
Cuts at: 0 1328 1947
Size: 1328 619
SfcI C'TryA_G
Cuts at: 0 284 1061 1104 1253 1368 1947
Size: 284 777 43 149 115 579
Fragments arranged by size:
777 579 284 149 115 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1604 1947
Size: 1604 343
SfoI GGC'GCC
Cuts at: 0 65 1947
Size: 65 1882
StyI C'CwwG_G
Cuts at: 0 114 893 1461 1706 1842 1947
Size: 114 779 568 245 136 105
Fragments arranged by size:
779 568 245 136 114 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 1211 1947
Size: 127 1084 736
Fragments arranged by size:
1084 736 127
TatI w'GTAC_w
Cuts at: 0 317 929 1232 1947
Size: 317 612 303 715
Fragments arranged by size:
715 612 317 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 968 1000 1838 1870 1947
Size: 574 32 362 32 838 32 77
Fragments arranged by size:
838 574 362 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1080 1947
Size: 1080 867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 835 1947
Size: 600 235 1112
Fragments arranged by size:
1112 600 235
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI PflMI PfoI
PpiI PspOMI PstI PvuII SacI SacII SapI SfcI
SfiI SfoI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AscI AseI AsiSI BaeI BamHI
BcgI BlpI BmtI BplI BpuEI BsaAI BsgI BsiWI
BspHI BsrBI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
DraIII DrdI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI XmaI XmnI ZraI