Restriction map for
pFN21AB6167
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstNI BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraIII EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRII EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvaI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AlwI 1494 GATCC GGATC(4/5)
AlwNI 1003 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1770 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1717 GGACC G^G[AT]CC
BcnI 164 CCGGG CC^[GC]GG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsmAI 1806 GTCTC GTCTC(1/5)
BsmBI 1805 CGTCTC CGTCTC(1/5)
BspDI 1936 ATCGAT AT^CGAT
BsrDI 173 GCAATG GCAATG(2/0)
BssKI 164 CCGGG ^CC[ACGT]GG
BssSI 1613 CTCGTG CACGAG(-5/-1)
BstAPI 1108 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1197 CCAAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 1717 GGACC G^G[ACGT]CC
ClaI 1936 ATCGAT AT^CGAT
DraII 1716 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1208 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1716 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1013 GAATTC G^AATTC
HaeIII 848 GGCC GG^CC
HapII 164 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HindIII 1315 AAGCTT A^AGCTT
HpaII 164 CCGG C^CGG
MspI 164 CCGG C^CGG
NciI 164 CCGGG CC^[GC]GG
PflFI 1770 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1716 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1476 CTGCAG CTGCA^G
Sau96I 1717 GGACC G^G[ACGT]CC
ScaI 1472 AGTACT AGT^ACT
ScrFI 164 CCGGG CC^[ACGT]GG
Tth111I 1770 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 197 CCGC CCGC(-3/-1)
726 GCGG
AfaI 1248 GTAC GT^AC
1473 GTAC
AseI 441 ATTAAT AT^TAAT
922 ATTAAT
AsnI 441 ATTAAT AT^TAAT
922 ATTAAT
BbsI 433 GAAGAC GAAGAC(2/6)
1669 GAAGAC
BbvI 1570 GCAGC GCAGC(8/12)
1879 GCTGC
BfaI 491 CTAG C^TAG
610 CTAG
BglII 1482 AGATCT A^GATCT
1620 AGATCT
BpmI 569 CTCCAG CTGGAG(16/14)
1624 CTGGAG
BpuAI 433 GAAGAC GAAGAC(2/6)
1669 GAAGAC
BsgI 1211 GTGCAG GTGCAG(16/14)
1568 GTGCAG
BsmFI 681 GGGAC GGGAC(10/14)
1305 GGGAC
Cac8I 169 GCAAGC GC[ACGT]^[ACGT]GC
1503 GCAGGC
DraI 259 TTTAAA TTT^AAA
450 TTTAAA
DsaI 220 CCATGG C^C[GA][CT]GG
1181 CCATGG
EcoNI 340 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1497 CCTGAAGCAGG
EcoT14I 220 CCATGG C^C[AT][AT]GG
1181 CCATGG
MaeI 491 CTAG C^TAG
610 CTAG
MslI 1018 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1381 CATTTTGATG
NcoI 220 CCATGG C^CATGG
1181 CCATGG
NlaIV 1044 GGAGCC GG[ACGT]^[ACGT]CC
1599 GGCTCC
PflMI 220 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
438 CCAATTAATGG
PleI 567 GACTC GAGTC(4/5)
976 GACTC
PshBI 441 ATTAAT AT^TAAT
922 ATTAAT
RsaI 1248 GTAC GT^AC
1473 GTAC
StyI 220 CCATGG C^C[AT][AT]GG
1181 CCATGG
TseI 1570 GCAGC G^C[AT]GC
1879 GCTGC
Tsp45I 565 GTGAC ^GT[GC]AC
1768 GTGAC
Van91I 220 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
438 CCAATTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BsaJI
BsiYI BslI BspMI BsrI BstF5I
BstYI DdeI DpnII Fnu4HI FokI
HinfI HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MwoI NdeII NlaIII NspI Sau3AI
SfaNI SfcI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I XhoII XmnI