(Circular) (Six-base) MAPSORT of: pFN21AB6167.seq Check: 6363 from: 1 to: 6652 pFN21AB6167 6652 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2206 3017 3584 2206 Size: 811 567 5274 Fragments arranged by size: 5274 811 567 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4462 278 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3995 1937 Size: 2058 4594 Acc65I G'GTAC_C Cuts at: 3973 3973 Size: 6652 AclI AA'CG_TT Cuts at: 1563 4779 5152 1563 Size: 3216 373 3063 Fragments arranged by size: 3216 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2916 3524 3881 4715 6099 6367 1632 Size: 641 643 608 357 834 1384 268 1917 Fragments arranged by size: 1917 1384 834 643 641 608 357 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6455 AflIII A'CryG_T Cuts at: 1203 5551 6528 1203 Size: 4348 977 1327 Fragments arranged by size: 4348 1327 977 AgeI A'CCGG_T Cuts at: 5483 5483 Size: 6652 AhdI GACnn_n'nnGTC Cuts at: 5381 5381 Size: 6652 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6652 AlwNI CAG_nnn'CTG Cuts at: 3013 4395 5967 3013 Size: 1382 1572 3698 Fragments arranged by size: 3698 1572 1382 ApaLI G'TGCA_C Cuts at: 4709 5865 4709 Size: 1156 5496 ApoI r'AATT_y Cuts at: 1493 2070 2076 2469 2805 3018 3096 3859 3961 Size: 577 6 393 336 213 78 763 102 Cuts at: 3961 4187 4339 6448 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 763 577 393 336 226 213 152 102 78 6 AseI AT'TA_AT Cuts at: 160 1032 2447 2928 5206 160 Size: 872 1415 481 2278 1606 Fragments arranged by size: 2278 1606 1415 872 481 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 AvaI C'yCGr_G Cuts at: 1870 1943 3977 1870 Size: 73 2034 4545 Fragments arranged by size: 4545 2034 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3647 3680 3647 Size: 33 6619 BamHI G'GATC_C Cuts at: 3982 4349 3982 Size: 367 6285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3973 5428 618 Size: 325 217 627 2186 1455 1842 Fragments arranged by size: 2186 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3971 1391 Size: 2580 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 3681 928 Size: 971 546 1236 3899 Fragments arranged by size: 3899 1236 971 546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4843 4877 1903 Size: 34 2906 34 3678 Fragments arranged by size: 3678 2906 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4544 5754 4544 Size: 1210 5442 BclI T'GATC_A Cuts at: 1548 1548 Size: 6652 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5263 136 Size: 107 122 71 631 605 3591 1525 Fragments arranged by size: 3591 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3487 3625 6647 3487 Size: 138 3022 3492 Fragments arranged by size: 3492 3022 138 BlpI GC'TnA_GC Cuts at: 4069 4069 Size: 6652 Bme1580I G_kGCm'C Cuts at: 1790 4713 5869 1790 Size: 2923 1156 2573 Fragments arranged by size: 2923 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3215 1318 Size: 1897 4755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4383 5341 442 Size: 416 53 83 399 24 2966 958 1753 Fragments arranged by size: 2966 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6652 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2661 2661 Size: 6652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 3650 5312 1239 Size: 1320 1091 1662 2579 Fragments arranged by size: 2579 1662 1320 1091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4777 5642 5940 4777 Size: 865 298 5489 Fragments arranged by size: 5489 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5315 882 Size: 617 3816 2219 Fragments arranged by size: 3816 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4443 493 Size: 711 3239 2702 Fragments arranged by size: 3239 2702 711 BsaBI GATnn'nnATC Cuts at: 6646 6646 Size: 6652 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4459 4841 5476 Size: 53 83 186 936 476 2450 382 635 Cuts at: 5476 275 Size: 1451 Fragments arranged by size: 2450 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5085 5483 5757 5904 6315 5085 Size: 398 274 147 411 5422 Fragments arranged by size: 5422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6427 Fragments arranged by size: 6427 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6652 BseYI C'CCAG_C Cuts at: 5855 6351 5855 Size: 496 6156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3237 3594 1163 Size: 573 1501 357 4221 Fragments arranged by size: 4221 1501 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4102 4863 5891 1540 Size: 275 186 2101 761 1028 2301 Fragments arranged by size: 2301 2101 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4713 4798 5869 4713 Size: 85 1071 5496 Fragments arranged by size: 5496 1071 85 BsmI GAATG_Cn' Cuts at: 4163 4256 4163 Size: 93 6559 BsmBI CGTCTCn'nnnn_ Cuts at: 3816 3816 Size: 6652 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3971 4713 4798 5869 1391 Size: 399 2181 742 85 1071 2174 Fragments arranged by size: 2181 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6315 6315 Size: 6652 BspHI T'CATG_A Cuts at: 4541 6271 4541 Size: 1730 4922 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 3017 3209 3584 4007 844 Size: 1362 811 192 375 423 3489 Fragments arranged by size: 3489 1362 811 423 375 192 BsrBI CCG'CTC Cuts at: 1394 1823 4539 1394 Size: 429 2716 3507 Fragments arranged by size: 3507 2716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 5147 5321 65 Size: 1183 937 2962 174 1396 Fragments arranged by size: 2962 1396 1183 937 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5296 5483 1700 Size: 110 3486 187 2869 Fragments arranged by size: 3486 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6652 BssSI C'ACGA_G Cuts at: 3618 4712 5724 3618 Size: 1094 1012 4546 Fragments arranged by size: 4546 1094 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3119 794 Size: 2325 4327 BstBI TT'CG_AA Cuts at: 5456 5456 Size: 6652 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6652 BstXI CCAn_nnnn'nTGG Cuts at: 3209 3209 Size: 6652 BstYI r'GATC_y Cuts at: 1972 3487 3498 3625 3982 4349 4734 4751 6203 Size: 1515 11 127 357 367 385 17 1452 Cuts at: 6203 6289 6301 6647 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1515 1452 385 367 357 346 127 86 17 12 11 BtgI C'CryG_G Cuts at: 513 1065 2004 2225 3186 513 Size: 552 939 221 961 3979 Fragments arranged by size: 3979 961 939 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5913 Fragments arranged by size: 5913 683 56 BtsI GCAGTG_nn' Cuts at: 2153 2668 4168 4962 4982 2153 Size: 515 1500 794 20 3823 Fragments arranged by size: 3823 1500 794 515 20 ClaI AT'CG_AT Cuts at: 1554 3942 1554 Size: 2388 4264 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6617 DraI TTT'AAA Cuts at: 2266 2457 3956 2266 Size: 191 1499 4962 Fragments arranged by size: 4962 1499 191 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3219 5659 809 Size: 507 1903 2440 1802 Fragments arranged by size: 2440 1903 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4099 4988 6617 Size: 54 1425 50 275 18 2269 889 1629 Cuts at: 6617 8 Size: 43 Fragments arranged by size: 2269 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4099 1537 Size: 275 2287 4090 Fragments arranged by size: 4090 2287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4582 1353 Size: 3229 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5239 5613 5759 387 Size: 1064 138 3650 374 146 1280 Fragments arranged by size: 3650 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2559 2916 3524 3650 3881 4715 Size: 393 641 286 357 608 126 231 834 Cuts at: 4715 5312 6099 6367 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 834 787 641 608 597 393 357 286 268 231 126 EcoNI CCTnn'n_nnAGG Cuts at: 2349 3506 2349 Size: 1157 5495 EcoO109I rG'GnC_Cy Cuts at: 3722 3722 Size: 6652 EcoRI G'AATT_C Cuts at: 3018 3961 4339 3018 Size: 943 378 5331 Fragments arranged by size: 5331 943 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3752 3752 Size: 6652 FspI TGC'GCA Cuts at: 5158 5158 Size: 6652 HaeII r_GCGC'y Cuts at: 1678 5799 1678 Size: 4121 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 3320 3352 3477 Size: 32 135 32 1210 32 506 32 125 Cuts at: 3477 3509 5293 5325 5367 5399 1373 Size: 32 1784 32 42 32 2626 Fragments arranged by size: 2626 1784 1210 506 135 125 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3996 4242 669 Size: 988 281 2058 246 3079 Fragments arranged by size: 3079 2058 988 281 246 HindIII A'AGCT_T Cuts at: 748 3320 748 Size: 2572 4080 HpaI GTT'AAC Cuts at: 4242 4242 Size: 6652 KpnI G_GTAC'C Cuts at: 3977 3977 Size: 6652 MfeI C'AATT_G Cuts at: 4251 4251 Size: 6652 MluI A'CGCG_T Cuts at: 6528 6528 Size: 6652 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5766 5950 1305 Size: 4461 184 2007 Fragments arranged by size: 4461 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4830 Fragments arranged by size: 4830 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3027 3390 4610 4969 5128 518 Size: 648 1861 363 1220 359 159 2042 Fragments arranged by size: 2042 1861 1220 648 363 359 159 MspA1I CmG'CkG Cuts at: 1606 4068 4745 5893 6138 1606 Size: 2462 677 1148 245 2120 Fragments arranged by size: 2462 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2225 3186 513 Size: 552 939 221 961 3979 Fragments arranged by size: 3979 961 939 552 221 NdeI CA'TA_TG Cuts at: 387 387 Size: 6652 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6652 NotI GC'GGCC_GC Cuts at: 4099 4099 Size: 6652 NspI r_CATG'y Cuts at: 1132 1229 2063 2513 3417 3758 3895 4010 5555 Size: 97 834 450 904 341 137 115 1545 Cuts at: 5555 1132 Size: 2229 Fragments arranged by size: 2229 1545 904 834 450 341 137 115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6652 PciI A'CATG_T Cuts at: 5551 5551 Size: 6652 PflMI CCAn_nnn'nTGG Cuts at: 1108 2231 2449 1108 Size: 1123 218 5311 Fragments arranged by size: 5311 1123 218 PmeI GTTT'AAAC Cuts at: 3956 3956 Size: 6652 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4721 4753 6234 6266 4721 Size: 32 1481 32 5107 Fragments arranged by size: 5107 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3722 3722 Size: 6652 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6652 PsiI TTA'TAA Cuts at: 2709 4222 2709 Size: 1513 5139 PstI C_TGCA'G Cuts at: 830 3485 4004 830 Size: 2655 519 3478 Fragments arranged by size: 3478 2655 519 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6652 SalI G'TCGA_C Cuts at: 1936 3994 1936 Size: 2058 4594 SbfI CC_TGCA'GG Cuts at: 4004 4004 Size: 6652 ScaI AGT'ACT Cuts at: 3479 4900 6612 3479 Size: 1421 1712 3519 Fragments arranged by size: 3519 1712 1421 SfcI C'TryA_G Cuts at: 826 1045 2346 2674 3481 3679 4000 5135 5816 Size: 219 1301 328 807 198 321 1135 681 Cuts at: 5816 6007 826 Size: 191 1471 Fragments arranged by size: 1471 1301 1135 807 681 328 321 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6652 SmaI CCC'GGG Cuts at: 1872 3979 1872 Size: 2107 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4756 5657 5919 820 Size: 197 926 2813 901 262 1553 Fragments arranged by size: 2813 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4443 493 Size: 3950 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6652 SphI G_CATG'C Cuts at: 1132 4010 1132 Size: 2878 3774 SspI AAT'ATT Cuts at: 5 52 4576 5 Size: 47 4524 2081 Fragments arranged by size: 4524 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6652 StyI C'CwwG_G Cuts at: 513 1065 2004 2225 3186 4091 513 Size: 552 939 221 961 905 3074 Fragments arranged by size: 3074 961 939 905 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4679 4696 4849 5034 5479 325 Size: 1255 3099 17 153 185 445 1498 Fragments arranged by size: 3099 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 3477 4898 Size: 274 80 33 51 1443 1275 225 1421 Cuts at: 4898 6610 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1421 1275 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6612 6644 6612 Size: 32 6620 Tth111I GACn'n_nGTC Cuts at: 1316 3778 1316 Size: 2462 4190 XbaI T'CTAG_A Cuts at: 3988 3988 Size: 6652 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6652 XmaI C'CCGG_G Cuts at: 1870 3977 1870 Size: 2107 4545 XmnI GAAnn'nnTTC Cuts at: 2220 3283 3794 4781 2220 Size: 1063 511 987 4091 Fragments arranged by size: 4091 1063 987 511 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4460 276 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI