Restriction map for
pFN21AB6167
(Linear) (Six-base) MAPSORT of: KIBB6167.seq Check: 4878 from: 1 to: 1947
KIBB6167.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 201 1012 1579 1947
Size: 201 811 567 368
Fragments arranged by size:
811 567 368 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 911 1519 1876 1947
Size: 268 643 608 357 71
Fragments arranged by size:
643 608 357 268 71
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 1947
Size: 1008 939
ApoI r'AATT_y
Cuts at: 0 65 71 464 800 1013 1091 1854 1947
Size: 65 6 393 336 213 78 763 93
Fragments arranged by size:
763 393 336 213 93 78 65 6
AseI AT'TA_AT
Cuts at: 0 442 923 1947
Size: 442 481 1024
Fragments arranged by size:
1024 481 442
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1642 1675 1947
Size: 1642 33 272
Fragments arranged by size:
1642 272 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 1676 1947
Size: 440 1236 271
Fragments arranged by size:
1236 440 271
BglII A'GATC_T
Cuts at: 0 1482 1620 1947
Size: 1482 138 327
Fragments arranged by size:
1482 327 138
BmgBI CAC'GTC
Cuts at: 0 1210 1947
Size: 1210 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 656 1947
Size: 656 1291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1645 1947
Size: 554 1091 302
Fragments arranged by size:
1091 554 302
BsaHI Gr'CG_yC
Cuts at: 0 4 1947
Size: 4 1943
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1589 1947
Size: 1232 357 358
Fragments arranged by size:
1232 358 357
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1811 1947
Size: 1811 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 1012 1204 1579 1947
Size: 201 811 192 375 368
Fragments arranged by size:
811 375 368 201 192
BsrDI GCAATG_nn'
Cuts at: 0 180 1947
Size: 180 1767
BssSI C'ACGA_G
Cuts at: 0 1613 1947
Size: 1613 334
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1114 1947
Size: 1114 833
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1204 1947
Size: 1204 743
BstYI r'GATC_y
Cuts at: 0 1482 1493 1620 1947
Size: 1482 11 127 327
Fragments arranged by size:
1482 327 127 11
BtgI C'CryG_G
Cuts at: 0 220 1181 1947
Size: 220 961 766
Fragments arranged by size:
961 766 220
BtsI GCAGTG_nn'
Cuts at: 0 148 663 1947
Size: 148 515 1284
Fragments arranged by size:
1284 515 148
ClaI AT'CG_AT
Cuts at: 0 1937 1947
Size: 1937 10
DraI TTT'AAA
Cuts at: 0 261 452 1947
Size: 261 191 1495
Fragments arranged by size:
1495 261 191
DrdI GACnn_nn'nnGTC
Cuts at: 0 1214 1947
Size: 1214 733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 554 911 1519 1645 1876 1947
Size: 268 286 357 608 126 231 71
Fragments arranged by size:
608 357 286 268 231 126 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 344 1501 1947
Size: 344 1157 446
Fragments arranged by size:
1157 446 344
EcoO109I rG'GnC_Cy
Cuts at: 0 1717 1947
Size: 1717 230
EcoRI G'AATT_C
Cuts at: 0 1013 1947
Size: 1013 934
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1747 1947
Size: 1747 200
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 1315 1347 1472 1504 1947
Size: 777 32 506 32 125 32 443
Fragments arranged by size:
777 506 443 125 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1315 1947
Size: 1315 632
MslI CAynn'nnrTG
Cuts at: 0 1022 1385 1947
Size: 1022 363 562
Fragments arranged by size:
1022 562 363
NcoI C'CATG_G
Cuts at: 0 220 1181 1947
Size: 220 961 766
Fragments arranged by size:
961 766 220
NspI r_CATG'y
Cuts at: 0 58 508 1412 1753 1890 1947
Size: 58 450 904 341 137 57
Fragments arranged by size:
904 450 341 137 58 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 226 444 1947
Size: 226 218 1503
Fragments arranged by size:
1503 226 218
PpuMI rG'GwC_Cy
Cuts at: 0 1717 1947
Size: 1717 230
PsiI TTA'TAA
Cuts at: 0 704 1947
Size: 704 1243
PstI C_TGCA'G
Cuts at: 0 1480 1947
Size: 1480 467
ScaI AGT'ACT
Cuts at: 0 1474 1947
Size: 1474 473
SfcI C'TryA_G
Cuts at: 0 341 669 1476 1674 1947
Size: 341 328 807 198 273
Fragments arranged by size:
807 341 328 273 198
StyI C'CwwG_G
Cuts at: 0 220 1181 1947
Size: 220 961 766
Fragments arranged by size:
961 766 220
TatI w'GTAC_w
Cuts at: 0 1247 1472 1947
Size: 1247 225 475
Fragments arranged by size:
1247 475 225
Tth111I GACn'n_nGTC
Cuts at: 0 1773 1947
Size: 1773 174
XmnI GAAnn'nnTTC
Cuts at: 0 215 1278 1789 1947
Size: 215 1063 511 158
Fragments arranged by size:
1063 511 215 158
Enzymes that do cut:
AarI AcuI AlwNI ApoI AseI BaeI BbsI BglII
BmgBI BplI BpmI BsaHI BsgI BsmBI BspMI BsrDI
BssSI BstAPI BstXI BstYI BtgI BtsI ClaI DraI
DrdI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HindIII
MslI NcoI NspI PflMI PpuMI PsiI PstI ScaI
SfcI StyI TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraIII EaeI EagI EarI EciI EcoICRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XcmI XhoI XmaI ZraI