Restriction map for
pFN21AB6162
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AvrII
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII BstBI BstEII BstPI
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuII RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 1092 GTATAC GT^[AC][GT]AC
AcsI 214 GAATTC [GA]^AATT[CT]
AflIII 266 ACATGT A^C[GA][CT]GT
AlwNI 1669 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1878 CCTCAGG CC^T[ACGT]AGG
ApoI 214 GAATTC [GA]^AATT[CT]
Asp700I 210 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 853 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1694 TGCGCA TGC^GCA
BamHI 1301 GGATCC G^GATCC
BanII 1486 GAGCTC G[GA]GC[CT]^C
BbeI 865 GGCGCC GGCGC^C
BbrPI 18 CACGTG CAC^GTG
BcgI 1154 GCAAGATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1928 AGATCT A^GATCT
BsaAI 18 CACGTG [CT]AC^GT[GA]
BsaI 616 GAGACC GGTCTC(1/5)
BsiEI 252 CGATCG CG[GA][CT]^CG
BsmBI 1515 CGTCTC CGTCTC(1/5)
BsmI 426 GAATGC GAATGC(1/-1)
BspHI 788 TCATGA T^CATGA
BspLU11I 266 ACATGT A^CATGT
BspMI 644 GCAGGT ACCTGC(4/8)
BssSI 540 CACGAG CACGAG(-5/-1)
Bst1107I 1092 GTATAC GTA^TAC
BstXI 605 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1092 GTATAC GTA^TAC
Bsu36I 1878 CCTCAGG CC^T[ACGT]AGG
DraIII 1178 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1878 CCTCAGG CC^T[ACGT]AGG
EcoNI 438 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 214 GAATTC G^AATTC
EcoT14I 1001 CCATGG C^C[AT][AT]GG
FspI 1694 TGCGCA TGC^GCA
HgaI 1187 GCGTC GACGC(5/10)
KasI 865 GGCGCC G^GCGCC
KspI 630 CCGCGG CCGC^GG
NaeI 1867 GCCGGC GCC^GGC
NarI 865 GGCGCC GG^CGCC
NcoI 1001 CCATGG C^CATGG
NgoMIV 1867 GCCGGC G^CCGGC
NruI 255 TCGCGA TCG^CGA
PaeR7I 107 CTCGAG C^TCGAG
PflFI 853 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1560 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 232 GACTC GAGTC(4/5)
PmaCI 18 CACGTG CAC^GTG
PmlI 18 CACGTG CAC^GTG
PstI 1339 CTGCAG CTGCA^G
PvuI 252 CGATCG CGAT^CG
RcaI 788 TCATGA T^CATGA
SacI 1486 GAGCTC GAGCT^C
SacII 630 CCGCGG CCGC^GG
SapI 346 GAAGAGC GCTCTTC(1/4)
ScaI 242 AGTACT AGT^ACT
SfcI 1339 CTGCAG C^T[GA][CT]AG
SfoI 865 GGCGCC GGC^GCC
SphI 1664 GCATGC GCATG^C
StyI 1001 CCATGG C^C[AT][AT]GG
Tsp509I 215 AATT ^AATT
Tth111I 853 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1560 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1452 TCTAGA T^CTAGA
XhoI 107 CTCGAG C^TCGAG
XmnI 210 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 1321 GGTACC G^GTACC
1897 GGTACC
AcyI 865 GGCGCC G[GA]^CG[CT]C
1186 GGCGTC
AlwI 1302 GATCC GGATC(4/5)
1301 GGATC
Asp718I 1321 GGTACC G^GTACC
1897 GGTACC
BfaI 379 CTAG C^TAG
1453 CTAG
BsaHI 865 GGCGCC G[GA]^CG[CT]C
1186 GGCGTC
BsgI 638 CTGCAC GTGCAG(16/14)
1444 CTGCAC
BsrBI 1719 CCGCTC CCGCTC(-3/-3)
138 GAGCGG
BsrFI 574 ACCGGC [GA]^CCGG[CT]
1867 GCCGGC
BsrI 1213 CCAGT ACTGG(1/-1)
1564 CCAGT
BstAPI 72 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1281 GCAGAAGGTGC
BstYI 1301 GGATCC [GA]^GATC[CT]
1928 AGATCT
Cfr10I 574 ACCGGC [GA]^CCGG[CT]
1867 GCCGGC
FauI 519 GCGGG CCCGC(4/6)
1257 GCGGG
Hin1I 865 GGCGCC G[GA]^CG[CT]C
1186 GGCGTC
KpnI 1321 GGTACC GGTAC^C
1897 GGTACC
MaeI 379 CTAG C^TAG
1453 CTAG
MflI 1301 GGATCC [GA]^GATC[CT]
1928 AGATCT
PpuMI 646 AGGTCCT [GA]G^G[AT]CC[CT]
1569 GGGACCC
TfiI 531 GAATC G^A[AT]TC
939 GATTC
XhoII 1301 GGATCC [GA]^GATC[CT]
1928 AGATCT
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BalI BanI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StuI TaqI TseI Tsp45I
TspRI TthHB8I