(Circular) (Six-base) MAPSORT of: pFN21AB6162.seq Check: 8579 from: 1 to: 6646 pFN21AB6162 6646 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 3989 1937 Size: 1161 891 4594 Fragments arranged by size: 4594 1161 891 Acc65I G'GTAC_C Cuts at: 3326 3902 3967 3326 Size: 576 65 6005 Fragments arranged by size: 6005 576 65 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3484 4709 6093 6361 1632 Size: 1852 1225 1384 268 1917 Fragments arranged by size: 1917 1852 1384 1225 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 2271 5545 6522 1203 Size: 1068 3274 977 1327 Fragments arranged by size: 3274 1327 1068 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 3554 1166 Size: 2388 4258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 1184 Size: 1338 5308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2109 2141 2109 Size: 32 6614 AlwNI CAG_nnn'CTG Cuts at: 3679 4389 5961 3679 Size: 710 1572 4364 Fragments arranged by size: 4364 1572 710 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 2219 3955 4181 4333 6442 1493 Size: 726 1736 226 152 2109 1697 Fragments arranged by size: 2109 1736 1697 726 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2112 2378 3920 3971 1870 Size: 73 97 72 266 1542 51 4545 Fragments arranged by size: 4545 1542 266 97 73 72 51 BamHI G'GATC_C Cuts at: 3306 3976 4343 3306 Size: 670 367 5609 Fragments arranged by size: 5609 670 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2845 2870 3139 3326 Size: 325 217 627 305 753 25 269 187 Cuts at: 3326 3902 3967 5422 618 Size: 576 65 1455 1842 Fragments arranged by size: 1842 1455 753 627 576 325 305 269 217 187 65 25 BanII G_rGCy'C Cuts at: 1391 3495 3965 1391 Size: 2104 470 4072 Fragments arranged by size: 4072 2104 470 BbeI G_GCGC'C Cuts at: 2874 2874 Size: 6646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3470 3552 3585 928 Size: 971 1571 82 33 3989 Fragments arranged by size: 3989 1571 971 82 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3148 3182 4837 4871 1903 Size: 34 1211 34 1655 34 3678 Fragments arranged by size: 3678 1655 1211 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5257 136 Size: 107 122 71 631 605 337 3248 1525 Fragments arranged by size: 3248 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3933 6641 3933 Size: 2708 3938 BlpI GC'TnA_GC Cuts at: 4063 4063 Size: 6646 Bme1580I G_kGCm'C Cuts at: 1790 2850 3056 3144 4707 5863 1790 Size: 1060 206 88 1563 1156 2573 Fragments arranged by size: 2573 1563 1156 1060 206 88 BmgBI CAC'GTC Cuts at: 1318 2809 1318 Size: 1491 5155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4377 5335 442 Size: 416 53 83 399 24 2960 958 1753 Fragments arranged by size: 2960 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2905 3712 3877 5306 1239 Size: 1666 807 165 1429 2579 Fragments arranged by size: 2579 1666 1429 807 165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 2945 2959 3389 4771 5636 5934 2641 Size: 304 14 430 1382 865 298 3353 Fragments arranged by size: 3353 1382 865 430 304 298 14 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 5309 882 Size: 617 1116 2694 2219 Fragments arranged by size: 2694 2219 1116 617 BsaAI yAC'GTr Cuts at: 493 1204 2025 4437 493 Size: 711 821 2412 2702 Fragments arranged by size: 2702 2412 821 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2871 3192 4453 4835 Size: 53 83 186 936 1338 321 1261 382 Cuts at: 4835 5470 275 Size: 635 1451 Fragments arranged by size: 1451 1338 1261 936 635 382 321 186 83 53 BsaWI w'CCGG_w Cuts at: 5079 5477 5751 5898 6309 5079 Size: 398 274 147 411 5416 Fragments arranged by size: 5416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2109 2139 3123 3153 1178 Size: 30 165 30 706 30 984 30 4671 Fragments arranged by size: 4671 984 706 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 2471 2981 1186 Size: 944 341 510 4851 Fragments arranged by size: 4851 944 510 341 BseYI C'CCAG_C Cuts at: 2073 2200 2482 2765 2980 3143 3564 5849 6345 Size: 127 282 283 215 163 421 2285 496 Cuts at: 6345 2073 Size: 2374 Fragments arranged by size: 2374 2285 496 421 283 282 215 163 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 3434 1163 Size: 573 892 806 4375 Fragments arranged by size: 4375 892 806 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2260 4096 4857 5885 1540 Size: 275 186 259 1836 761 1028 2301 Fragments arranged by size: 2301 1836 1028 761 275 259 186 BsiHKAI G_wGCw'C Cuts at: 2552 3283 3495 4707 4792 5863 2552 Size: 731 212 1212 85 1071 3335 Fragments arranged by size: 3335 1212 1071 731 212 85 BsmI GAATG_Cn' Cuts at: 2437 4157 4250 2437 Size: 1720 93 4833 Fragments arranged by size: 4833 1720 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3526 3526 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2552 2850 3056 3144 3283 3495 3965 Size: 399 762 298 206 88 139 212 470 Cuts at: 3965 4707 4792 5863 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 762 742 470 399 298 212 206 139 88 85 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 2793 4535 6265 2793 Size: 1742 1730 3174 Fragments arranged by size: 3174 1742 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2640 4001 844 Size: 1796 1361 3489 Fragments arranged by size: 3489 1796 1361 BsrBI CCG'CTC Cuts at: 1394 1823 2145 3726 4533 1394 Size: 429 322 1581 807 3507 Fragments arranged by size: 3507 1581 807 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 2314 3150 3201 5141 5315 65 Size: 1183 1066 836 51 1940 174 1396 Fragments arranged by size: 1940 1396 1183 1066 836 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 2579 3872 5290 5477 1700 Size: 110 769 1293 1418 187 2869 Fragments arranged by size: 2869 1418 1293 769 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 2545 4706 5718 2545 Size: 2161 1012 3473 Fragments arranged by size: 3473 2161 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 3292 794 Size: 1289 1209 4148 Fragments arranged by size: 4148 1289 1209 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2617 Size: 6646 BstYI r'GATC_y Cuts at: 1972 3306 3933 3976 4343 4728 4745 6197 6283 Size: 1334 627 43 367 385 17 1452 86 Cuts at: 6283 6295 6641 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1334 627 385 367 346 86 43 17 12 BstZ17I GTA'TAC Cuts at: 3099 3099 Size: 6646 Bsu36I CC'TnA_GG Cuts at: 3884 3884 Size: 6646 BtgI C'CryG_G Cuts at: 513 1065 2004 2321 2635 3006 3661 513 Size: 552 939 317 314 371 655 3498 Fragments arranged by size: 3498 939 655 552 371 317 314 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2575 2590 487 Size: 683 56 1349 15 4543 Fragments arranged by size: 4543 1349 683 56 15 BtsI GCAGTG_nn' Cuts at: 2058 3388 3645 4162 4956 4976 2058 Size: 1330 257 517 794 20 3728 Fragments arranged by size: 3728 1330 794 517 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 3188 3188 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2814 3009 Size: 54 1425 50 275 18 189 795 195 Cuts at: 3009 3532 4093 4982 6611 8 Size: 523 561 889 1629 43 Fragments arranged by size: 1629 1425 889 795 561 523 275 195 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 2400 3920 4576 1353 Size: 993 54 1520 656 3423 Fragments arranged by size: 3423 1520 993 656 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2267 3620 5233 5607 5753 387 Size: 1064 138 678 1353 1613 374 146 1280 Fragments arranged by size: 1613 1353 1280 1064 678 374 146 138 EcoICRI GAG'CTC Cuts at: 3493 3493 Size: 6646 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2905 3484 3712 3877 4709 5306 6093 Size: 393 1273 579 228 165 832 597 787 Cuts at: 6093 6361 1239 Size: 268 1524 Fragments arranged by size: 1524 1273 832 787 597 579 393 268 228 165 EcoNI CCTnn'n_nnAGG Cuts at: 2447 2447 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 2652 3364 3575 2652 Size: 712 211 5723 Fragments arranged by size: 5723 712 211 EcoRI G'AATT_C Cuts at: 2219 3955 4333 2219 Size: 1736 378 4532 Fragments arranged by size: 4532 1736 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 3085 3313 2239 Size: 846 228 5572 Fragments arranged by size: 5572 846 228 FspI TGC'GCA Cuts at: 3701 5152 3701 Size: 1451 5195 HaeII r_GCGC'y Cuts at: 1678 2155 2443 2874 3277 3622 5793 1678 Size: 477 288 431 403 345 2171 2531 Fragments arranged by size: 2531 2171 477 431 403 345 288 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2849 2881 5287 Size: 32 135 32 650 32 595 32 2406 Cuts at: 5287 5319 5361 5393 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2406 650 595 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KasI G'GCGC_C Cuts at: 2870 2870 Size: 6646 KpnI G_GTAC'C Cuts at: 3330 3906 3971 3330 Size: 576 65 6005 Fragments arranged by size: 6005 576 65 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3255 5760 5944 1305 Size: 1950 2505 184 2007 Fragments arranged by size: 2505 2007 1950 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 2816 3011 10 Size: 54 1425 343 189 795 195 3645 Fragments arranged by size: 3645 1425 795 343 195 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2594 2807 3032 3554 4604 4963 5122 Size: 648 1428 213 225 522 1050 359 159 Cuts at: 5122 518 Size: 2042 Fragments arranged by size: 2042 1428 1050 648 522 359 225 213 159 MspA1I CmG'CkG Cuts at: 1606 2515 2637 3262 3378 4062 4739 5887 6132 Size: 909 122 625 116 684 677 1148 245 Cuts at: 6132 1606 Size: 2120 Fragments arranged by size: 2120 1148 909 684 677 625 245 122 116 NaeI GCC'GGC Cuts at: 3874 3874 Size: 6646 NarI GG'CG_CC Cuts at: 2871 2871 Size: 6646 NcoI C'CATG_G Cuts at: 513 1065 2004 3006 513 Size: 552 939 1002 4153 Fragments arranged by size: 4153 1002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 3872 3872 Size: 6646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NruI TCG'CGA Cuts at: 2262 2262 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2275 3673 3688 4004 5549 1132 Size: 97 1046 1398 15 316 1545 2229 Fragments arranged by size: 2229 1545 1398 1046 316 97 15 PasI CC'CwG_GG Cuts at: 1348 3511 1348 Size: 2163 4483 PciI A'CATG_T Cuts at: 2271 5545 2271 Size: 3274 3372 PflMI CCAn_nnn'nTGG Cuts at: 1108 3571 1108 Size: 2463 4183 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PmlI CAC'GTG Cuts at: 2025 2025 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2109 2141 4715 4747 6228 6260 2109 Size: 32 2574 32 1481 32 2495 Fragments arranged by size: 2574 2495 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2652 3575 2652 Size: 923 5723 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PstI C_TGCA'G Cuts at: 830 3348 3998 830 Size: 2518 650 3478 Fragments arranged by size: 3478 2518 650 PvuI CG_AT'CG Cuts at: 2001 2260 2001 Size: 259 6387 SacI G_AGCT'C Cuts at: 3495 3495 Size: 6646 SacII CC_GC'GG Cuts at: 2638 2638 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SanDI GG'GwC_CC Cuts at: 3575 3575 Size: 6646 SapI GCTCTTCn'nnn_ Cuts at: 2346 2346 Size: 6646 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 2249 4894 6606 2249 Size: 2645 1712 2289 Fragments arranged by size: 2645 2289 1712 SfcI C'TryA_G Cuts at: 826 1045 3344 3994 5129 5810 6001 826 Size: 219 2299 650 1135 681 191 1471 Fragments arranged by size: 2299 1471 1135 681 650 219 191 SfoI GGC'GCC Cuts at: 2872 2872 Size: 6646 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2112 2656 2938 2960 3368 4750 Size: 197 926 169 544 282 22 408 1382 Cuts at: 4750 5651 5913 820 Size: 901 262 1553 Fragments arranged by size: 1553 1382 926 901 544 408 282 262 197 169 22 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 3673 4004 1132 Size: 2541 331 3774 Fragments arranged by size: 3774 2541 331 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 2016 2342 2693 1517 Size: 499 326 351 5470 Fragments arranged by size: 5470 499 351 326 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 4085 513 Size: 552 939 1002 1079 3074 Fragments arranged by size: 3074 1079 1002 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2433 2590 4673 4690 4843 5028 5473 Size: 1255 853 157 2083 17 153 185 445 Cuts at: 5473 325 Size: 1498 Fragments arranged by size: 2083 1498 1255 853 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2719 3348 Size: 274 80 33 51 1443 270 472 629 Cuts at: 3348 4892 6604 96 Size: 1544 1712 138 Fragments arranged by size: 1712 1544 1443 629 472 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 6606 6638 3123 Size: 32 3451 32 3131 Fragments arranged by size: 3451 3131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2861 1316 Size: 1545 5101 XbaI T'CTAG_A Cuts at: 3457 3982 3457 Size: 525 6121 XhoI C'TCGA_G Cuts at: 1943 2112 1943 Size: 169 6477 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 2219 4775 2219 Size: 2556 4090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI EcoRV FseI FspAI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI XcmI