Restriction map for
pFN21AB6162
(Linear) (Six-base) MAPSORT of: KIBB6162.seq Check: 8998 from: 1 to: 1941
KIBB6162.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1093 1941
Size: 1093 848
Acc65I G'GTAC_C
Cuts at: 0 1321 1897 1941
Size: 1321 576 44
Fragments arranged by size:
1321 576 44
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1479 1941
Size: 1479 462
AflIII A'CryG_T
Cuts at: 0 266 1941
Size: 266 1675
AleI CACnn'nnGTG
Cuts at: 0 1549 1941
Size: 1549 392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 1941
Size: 517 1424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 104 136 1941
Size: 104 32 1805
Fragments arranged by size:
1805 104 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1674 1941
Size: 1674 267
ApoI r'AATT_y
Cuts at: 0 214 1941
Size: 214 1727
AvaI C'yCGr_G
Cuts at: 0 35 107 373 1915 1941
Size: 35 72 266 1542 26
Fragments arranged by size:
1542 266 72 35 26
BamHI G'GATC_C
Cuts at: 0 1301 1941
Size: 1301 640
BanI G'GyrC_C
Cuts at: 0 87 840 865 1134 1321 1897 1941
Size: 87 753 25 269 187 576 44
Fragments arranged by size:
753 576 269 187 87 44 25
BanII G_rGCy'C
Cuts at: 0 1490 1941
Size: 1490 451
BbeI G_GCGC'C
Cuts at: 0 869 1941
Size: 869 1072
BbsI GAAGACnn'nnnn_
Cuts at: 0 1465 1547 1580 1941
Size: 1465 82 33 361
Fragments arranged by size:
1465 361 82 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1143 1177 1941
Size: 1143 34 764
Fragments arranged by size:
1143 764 34
BglII A'GATC_T
Cuts at: 0 1928 1941
Size: 1928 13
Bme1580I G_kGCm'C
Cuts at: 0 845 1051 1139 1941
Size: 845 206 88 802
Fragments arranged by size:
845 802 206 88
BmgBI CAC'GTC
Cuts at: 0 804 1941
Size: 804 1137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1707 1872 1941
Size: 900 807 165 69
Fragments arranged by size:
900 807 165 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 940 954 1384 1941
Size: 636 304 14 430 557
Fragments arranged by size:
636 557 430 304 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 1941
Size: 610 1331
BsaAI yAC'GTr
Cuts at: 0 20 1941
Size: 20 1921
BsaHI Gr'CG_yC
Cuts at: 0 866 1187 1941
Size: 866 321 754
Fragments arranged by size:
866 754 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 104 134 1118 1148 1941
Size: 104 30 984 30 793
Fragments arranged by size:
984 793 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 466 976 1941
Size: 125 341 510 965
Fragments arranged by size:
965 510 341 125
BseYI C'CCAG_C
Cuts at: 0 68 195 477 760 975 1138 1559 1941
Size: 68 127 282 283 215 163 421 382
Fragments arranged by size:
421 382 283 282 215 163 127 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1429 1941
Size: 623 806 512
Fragments arranged by size:
806 623 512
BsiEI CG_ry'CG
Cuts at: 0 255 1941
Size: 255 1686
BsiHKAI G_wGCw'C
Cuts at: 0 547 1278 1490 1941
Size: 547 731 212 451
Fragments arranged by size:
731 547 451 212
BsmI GAATG_Cn'
Cuts at: 0 432 1941
Size: 432 1509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1521 1941
Size: 1521 420
Bsp1286I G_dGCh'C
Cuts at: 0 547 845 1051 1139 1278 1490 1941
Size: 547 298 206 88 139 212 451
Fragments arranged by size:
547 451 298 212 206 139 88
BspHI T'CATG_A
Cuts at: 0 788 1941
Size: 788 1153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 635 1941
Size: 635 1306
BsrBI CCG'CTC
Cuts at: 0 140 1721 1941
Size: 140 1581 220
Fragments arranged by size:
1581 220 140
BsrDI GCAATG_nn'
Cuts at: 0 309 1145 1196 1941
Size: 309 836 51 745
Fragments arranged by size:
836 745 309 51
BsrFI r'CCGG_y
Cuts at: 0 574 1867 1941
Size: 574 1293 74
Fragments arranged by size:
1293 574 74
BssSI C'ACGA_G
Cuts at: 0 540 1941
Size: 540 1401
BstAPI GCAn_nnn'nTGC
Cuts at: 0 78 1287 1941
Size: 78 1209 654
Fragments arranged by size:
1209 654 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1941
Size: 612 1329
BstYI r'GATC_y
Cuts at: 0 1301 1928 1941
Size: 1301 627 13
Fragments arranged by size:
1301 627 13
BstZ17I GTA'TAC
Cuts at: 0 1094 1941
Size: 1094 847
Bsu36I CC'TnA_GG
Cuts at: 0 1879 1941
Size: 1879 62
BtgI C'CryG_G
Cuts at: 0 316 630 1001 1656 1941
Size: 316 314 371 655 285
Fragments arranged by size:
655 371 316 314 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 570 585 1941
Size: 570 15 1356
Fragments arranged by size:
1356 570 15
BtsI GCAGTG_nn'
Cuts at: 0 53 1383 1640 1941
Size: 53 1330 257 301
Fragments arranged by size:
1330 301 257 53
DraIII CAC_nnn'GTG
Cuts at: 0 1183 1941
Size: 1183 758
EaeI y'GGCC_r
Cuts at: 0 14 809 1004 1527 1941
Size: 14 795 195 523 414
Fragments arranged by size:
795 523 414 195 14
EarI CTCTTCn'nnn_
Cuts at: 0 341 395 1915 1941
Size: 341 54 1520 26
Fragments arranged by size:
1520 341 54 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 262 1615 1941
Size: 262 1353 326
Fragments arranged by size:
1353 326 262
EcoICRI GAG'CTC
Cuts at: 0 1488 1941
Size: 1488 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1479 1707 1872 1941
Size: 900 579 228 165 69
Fragments arranged by size:
900 579 228 165 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 1941
Size: 442 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 647 1359 1570 1941
Size: 647 712 211 371
Fragments arranged by size:
712 647 371 211
EcoRI G'AATT_C
Cuts at: 0 214 1941
Size: 214 1727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 1080 1308 1941
Size: 234 846 228 633
Fragments arranged by size:
846 633 234 228
FspI TGC'GCA
Cuts at: 0 1696 1941
Size: 1696 245
HaeII r_GCGC'y
Cuts at: 0 150 438 869 1272 1617 1941
Size: 150 288 431 403 345 324
Fragments arranged by size:
431 403 345 324 288 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 844 876 1941
Size: 217 32 595 32 1065
Fragments arranged by size:
1065 595 217 32 32
KasI G'GCGC_C
Cuts at: 0 865 1941
Size: 865 1076
KpnI G_GTAC'C
Cuts at: 0 1325 1901 1941
Size: 1325 576 40
Fragments arranged by size:
1325 576 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1941
Size: 1250 691
MscI TGG'CCA
Cuts at: 0 16 811 1006 1941
Size: 16 795 195 935
Fragments arranged by size:
935 795 195 16
MslI CAynn'nnrTG
Cuts at: 0 589 802 1027 1549 1941
Size: 589 213 225 522 392
Fragments arranged by size:
589 522 392 225 213
MspA1I CmG'CkG
Cuts at: 0 510 632 1257 1373 1941
Size: 510 122 625 116 568
Fragments arranged by size:
625 568 510 122 116
NaeI GCC'GGC
Cuts at: 0 1869 1941
Size: 1869 72
NarI GG'CG_CC
Cuts at: 0 866 1941
Size: 866 1075
NcoI C'CATG_G
Cuts at: 0 1001 1941
Size: 1001 940
NgoMIV G'CCGG_C
Cuts at: 0 1867 1941
Size: 1867 74
NruI TCG'CGA
Cuts at: 0 257 1941
Size: 257 1684
NspI r_CATG'y
Cuts at: 0 270 1668 1683 1941
Size: 270 1398 15 258
Fragments arranged by size:
1398 270 258 15
PasI CC'CwG_GG
Cuts at: 0 1506 1941
Size: 1506 435
PciI A'CATG_T
Cuts at: 0 266 1941
Size: 266 1675
PflMI CCAn_nnn'nTGG
Cuts at: 0 1566 1941
Size: 1566 375
PmlI CAC'GTG
Cuts at: 0 20 1941
Size: 20 1921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 1941
Size: 104 32 1805
Fragments arranged by size:
1805 104 32
PpuMI rG'GwC_Cy
Cuts at: 0 647 1570 1941
Size: 647 923 371
Fragments arranged by size:
923 647 371
PstI C_TGCA'G
Cuts at: 0 1343 1941
Size: 1343 598
PvuI CG_AT'CG
Cuts at: 0 255 1941
Size: 255 1686
SacI G_AGCT'C
Cuts at: 0 1490 1941
Size: 1490 451
SacII CC_GC'GG
Cuts at: 0 633 1941
Size: 633 1308
SanDI GG'GwC_CC
Cuts at: 0 1570 1941
Size: 1570 371
SapI GCTCTTCn'nnn_
Cuts at: 0 341 1941
Size: 341 1600
ScaI AGT'ACT
Cuts at: 0 244 1941
Size: 244 1697
SfcI C'TryA_G
Cuts at: 0 1339 1941
Size: 1339 602
SfoI GGC'GCC
Cuts at: 0 867 1941
Size: 867 1074
SmlI C'TyrA_G
Cuts at: 0 107 651 933 955 1363 1941
Size: 107 544 282 22 408 578
Fragments arranged by size:
578 544 408 282 107 22
SphI G_CATG'C
Cuts at: 0 1668 1941
Size: 1668 273
StuI AGG'CCT
Cuts at: 0 11 337 688 1941
Size: 11 326 351 1253
Fragments arranged by size:
1253 351 326 11
StyI C'CwwG_G
Cuts at: 0 1001 1941
Size: 1001 940
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 428 585 1941
Size: 428 157 1356
Fragments arranged by size:
1356 428 157
TatI w'GTAC_w
Cuts at: 0 242 714 1343 1941
Size: 242 472 629 598
Fragments arranged by size:
629 598 472 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1941
Size: 1118 32 791
Fragments arranged by size:
1118 791 32
Tth111I GACn'n_nGTC
Cuts at: 0 856 1941
Size: 856 1085
XbaI T'CTAG_A
Cuts at: 0 1452 1941
Size: 1452 489
XhoI C'TCGA_G
Cuts at: 0 107 1941
Size: 107 1834
XmnI GAAnn'nnTTC
Cuts at: 0 214 1941
Size: 214 1727
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BglII Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PmlI PpiI PpuMI PstI PvuI
SacI SacII SanDI SapI ScaI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I
BsaBI BsaWI BsiWI BspEI BsrGI BssHII BstBI BstEII
ClaI CspCI DraI DrdI EagI EcoRV FseI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NsiI PacI PfoI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuII RsrII SalI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XcmI
XmaI ZraI