(Circular) (Six-base) MAPSORT of: pFN21AB6153.seq Check: 123 from: 1 to: 6496 pFN21AB6153 6496 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3226 3226 Size: 6496 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3688 3839 1937 Size: 1751 151 4594 Fragments arranged by size: 4594 1751 151 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2460 2664 2777 3116 3248 3452 4559 5943 Size: 828 204 113 339 132 204 1107 1384 Cuts at: 5943 6211 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1107 828 339 268 204 204 132 113 AflII C'TTAA_G Cuts at: 820 1017 2654 820 Size: 197 1637 4662 Fragments arranged by size: 4662 1637 197 AflIII A'CryG_T Cuts at: 1203 2721 5395 6372 1203 Size: 1518 2674 977 1327 Fragments arranged by size: 2674 1518 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AlwNI CAG_nnn'CTG Cuts at: 3185 3241 3638 4239 5811 3185 Size: 56 397 601 1572 3870 Fragments arranged by size: 3870 1572 601 397 56 ApaLI G'TGCA_C Cuts at: 3293 4553 5709 3293 Size: 1260 1156 4080 Fragments arranged by size: 4080 1260 1156 ApoI r'AATT_y Cuts at: 1493 2260 2600 2686 2714 3805 4031 4183 6292 Size: 767 340 86 28 1091 226 152 2109 Cuts at: 6292 1493 Size: 1697 Fragments arranged by size: 2109 1697 1091 767 340 226 152 86 28 AseI AT'TA_AT Cuts at: 160 1032 2190 5050 160 Size: 872 1158 2860 1606 Fragments arranged by size: 2860 1606 1158 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 3821 1870 Size: 73 1878 4545 Fragments arranged by size: 4545 1878 73 BamHI G'GATC_C Cuts at: 3626 3826 4193 3626 Size: 200 367 5929 Fragments arranged by size: 5929 367 200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3353 3413 3817 5272 618 Size: 325 217 627 1566 60 404 1455 1842 Fragments arranged by size: 1842 1566 1455 627 404 325 217 60 BanII G_rGCy'C Cuts at: 1391 3260 3815 1391 Size: 1869 555 4072 Fragments arranged by size: 4072 1869 555 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2166 2466 2963 3375 928 Size: 971 267 300 497 412 4049 Fragments arranged by size: 4049 971 497 412 300 267 BbvCI CC'TCA_GC Cuts at: 2302 2302 Size: 6496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4687 4721 1903 Size: 34 2750 34 3678 Fragments arranged by size: 3678 2750 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BfrBI ATG'CAT Cuts at: 2569 3778 2569 Size: 1209 5287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2405 5107 136 Size: 107 122 71 631 605 733 2702 1525 Fragments arranged by size: 2702 1525 733 631 605 122 107 71 BglII A'GATC_T Cuts at: 2764 3708 6491 2764 Size: 944 2783 2769 Fragments arranged by size: 2783 2769 944 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 2531 3297 4557 5713 1790 Size: 741 766 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 766 741 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3183 3183 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 5156 1239 Size: 842 3075 2579 Fragments arranged by size: 3075 2579 842 Bpu10I CC'TnA_GC Cuts at: 2302 2302 Size: 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4621 5486 5784 4621 Size: 865 298 5333 Fragments arranged by size: 5333 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5159 882 Size: 617 3660 2219 Fragments arranged by size: 3660 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 4287 493 Size: 711 1518 1565 2702 Fragments arranged by size: 2702 1565 1518 711 BsaBI GATnn'nnATC Cuts at: 3639 6490 3639 Size: 2851 3645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4303 4685 5320 275 Size: 53 83 186 936 2770 382 635 1451 Fragments arranged by size: 2770 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2912 2942 3337 3367 1178 Size: 30 165 30 1509 30 395 30 4307 Fragments arranged by size: 4307 1509 395 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2936 1186 Size: 1750 4746 BseYI C'CCAG_C Cuts at: 5699 6195 5699 Size: 496 6000 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2730 1163 Size: 573 994 4929 Fragments arranged by size: 4929 994 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3946 4707 5735 1540 Size: 275 186 913 1032 761 1028 2301 Fragments arranged by size: 2301 1032 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3297 4557 4642 5713 3297 Size: 1260 85 1071 4080 Fragments arranged by size: 4080 1260 1071 85 BsmI GAATG_Cn' Cuts at: 3116 4007 4100 3116 Size: 891 93 5512 Fragments arranged by size: 5512 891 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2531 3260 3297 3815 4557 4642 5713 Size: 399 741 729 37 518 742 85 1071 Cuts at: 5713 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 741 729 518 399 85 37 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3226 3851 844 Size: 2382 625 3489 Fragments arranged by size: 3489 2382 625 BsrBI CCG'CTC Cuts at: 1394 1823 4383 1394 Size: 429 2560 3507 Fragments arranged by size: 3507 2560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5140 5327 1700 Size: 110 3330 187 2869 Fragments arranged by size: 3330 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2269 96 Size: 2173 4323 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6496 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstXI CCAn_nnnn'nTGG Cuts at: 2433 2902 3683 2433 Size: 469 781 5246 Fragments arranged by size: 5246 781 469 BstYI r'GATC_y Cuts at: 1972 2764 3626 3708 3826 4193 4578 4595 6047 Size: 792 862 82 118 367 385 17 1452 Cuts at: 6047 6133 6145 6491 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 862 792 385 367 346 118 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 3158 3158 Size: 6496 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5005 Fragments arranged by size: 5005 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3737 487 Size: 683 56 2511 3246 Fragments arranged by size: 3246 2511 683 56 BtsI GCAGTG_nn' Cuts at: 2507 2726 2907 2958 4012 4806 4826 2507 Size: 219 181 51 1054 794 20 4177 Fragments arranged by size: 4177 1054 794 219 181 51 20 ClaI AT'CG_AT Cuts at: 1554 2467 1554 Size: 913 5583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3496 3800 3496 Size: 304 6192 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2079 5503 809 Size: 507 763 3424 1802 Fragments arranged by size: 3424 1802 763 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2911 3787 3943 Size: 54 1425 50 275 18 1081 876 156 Cuts at: 3943 4832 6461 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1081 889 876 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 3943 1537 Size: 275 1099 1032 4090 Fragments arranged by size: 4090 1099 1032 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2865 2940 3044 4426 1353 Size: 1512 75 104 1382 3423 Fragments arranged by size: 3423 1512 1382 104 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2460 2664 2777 3116 3248 3452 Size: 393 449 379 204 113 339 132 204 Cuts at: 3452 4559 5156 5943 6211 1239 Size: 1107 597 787 268 1524 Fragments arranged by size: 1524 1107 787 597 449 393 379 339 268 204 204 132 113 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 6496 EcoRI G'AATT_C Cuts at: 2260 3805 4183 2260 Size: 1545 378 4573 Fragments arranged by size: 4573 1545 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2669 2179 Size: 490 6006 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 5643 1678 Size: 3965 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 2843 2875 3554 Size: 32 135 32 884 32 355 32 679 Cuts at: 3554 3586 3611 3643 5137 5169 5211 5243 1373 Size: 32 25 32 1494 32 42 32 2626 Fragments arranged by size: 2626 1494 884 679 355 135 42 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3529 3840 4086 669 Size: 988 281 1591 311 246 3079 Fragments arranged by size: 3079 1591 988 311 281 246 HindIII A'AGCT_T Cuts at: 748 3098 748 Size: 2350 4146 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 3036 5610 5794 1305 Size: 1137 594 2574 184 2007 Fragments arranged by size: 2574 2007 1137 594 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3789 10 Size: 54 1425 343 1957 2717 Fragments arranged by size: 2717 1957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2808 3269 4454 4813 4972 518 Size: 648 1642 461 1185 359 159 2042 Fragments arranged by size: 2042 1642 1185 648 461 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 3226 3912 4589 5737 5982 1606 Size: 453 1167 686 677 1148 245 2120 Fragments arranged by size: 2120 1167 1148 686 677 453 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5005 Fragments arranged by size: 5005 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NsiI A_TGCA'T Cuts at: 2571 3780 2571 Size: 1209 5287 NspI r_CATG'y Cuts at: 1132 1229 2573 3773 3854 5399 1132 Size: 97 1344 1200 81 1545 2229 Fragments arranged by size: 2229 1545 1344 1200 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6496 PfoI T'CCnGG_A Cuts at: 2043 2043 Size: 6496 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PmlI CAC'GTG Cuts at: 2722 2722 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4565 4597 6078 6110 4565 Size: 32 1481 32 4951 Fragments arranged by size: 4951 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 2541 3150 4066 2541 Size: 609 916 4971 Fragments arranged by size: 4971 916 609 PstI C_TGCA'G Cuts at: 830 3237 3848 830 Size: 2407 611 3478 Fragments arranged by size: 3478 2407 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2059 3226 2059 Size: 1167 5329 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SapI GCTCTTCn'nnn_ Cuts at: 2865 2865 Size: 6496 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SfcI C'TryA_G Cuts at: 826 1045 3233 3844 4979 5660 5851 826 Size: 219 2188 611 1135 681 191 1471 Fragments arranged by size: 2188 1471 1135 681 611 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 4600 5501 5763 820 Size: 197 926 711 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 2573 3854 1132 Size: 1441 1281 3774 Fragments arranged by size: 3774 1441 1281 SspI AAT'ATT Cuts at: 5 52 3599 4420 5 Size: 47 3547 821 2081 Fragments arranged by size: 3547 2081 821 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2004 3682 3935 513 Size: 552 939 1678 253 3074 Fragments arranged by size: 3074 1678 939 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4523 4540 4693 4878 5323 325 Size: 1255 2943 17 153 185 445 1498 Fragments arranged by size: 2943 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 2269 4742 Size: 274 80 33 51 1443 150 142 2473 Cuts at: 4742 6454 96 Size: 1712 138 Fragments arranged by size: 2473 1712 1443 274 150 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2797 2829 2912 2944 3337 3369 6456 6488 2797 Size: 32 83 32 393 32 3087 32 2805 Fragments arranged by size: 3087 2805 393 83 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 2122 3832 2122 Size: 1710 4786 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 2620 2455 Size: 165 6331 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI