(Circular) (Six-base) MAPSORT of: pFN21AB6151.seq Check: 5247 from: 1 to: 6493 pFN21AB6151 6493 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3126 4556 5940 6208 1632 Size: 1494 1430 1384 268 1917 Fragments arranged by size: 1917 1494 1430 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 2043 5392 6369 1203 Size: 840 3349 977 1327 Fragments arranged by size: 3349 1327 977 840 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2177 2209 2177 Size: 32 6461 AlwNI CAG_nnn'CTG Cuts at: 3589 4236 5808 3589 Size: 647 1572 4274 Fragments arranged by size: 4274 1572 647 ApaI G_GGCC'C Cuts at: 3567 3567 Size: 6493 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 2656 2760 2804 2872 3802 4028 4180 6289 Size: 1163 104 44 68 930 226 152 2109 Cuts at: 6289 1493 Size: 1697 Fragments arranged by size: 2109 1697 1163 930 226 152 104 68 44 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 3818 1870 Size: 73 1875 4545 Fragments arranged by size: 4545 1875 73 BamHI G'GATC_C Cuts at: 2399 3823 4190 2399 Size: 1424 367 4702 Fragments arranged by size: 4702 1424 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3814 5269 618 Size: 325 217 627 2027 1455 1842 Fragments arranged by size: 2027 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2889 3053 3264 3567 3812 1391 Size: 1498 164 211 303 245 4072 Fragments arranged by size: 4072 1498 303 245 211 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3114 928 Size: 971 1215 4307 Fragments arranged by size: 4307 1215 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3033 3067 4684 4718 1903 Size: 34 1096 34 1617 34 3678 Fragments arranged by size: 3678 1617 1096 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 2622 3084 3213 1548 Size: 1074 462 129 4828 Fragments arranged by size: 4828 1074 462 129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3526 5104 Size: 107 122 71 631 605 337 1517 1578 Cuts at: 5104 136 Size: 1525 Fragments arranged by size: 1578 1525 1517 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2431 2577 6488 2431 Size: 146 3911 2436 Fragments arranged by size: 3911 2436 146 BlpI GC'TnA_GC Cuts at: 2936 3910 2936 Size: 974 5519 Bme1580I G_kGCm'C Cuts at: 1790 3567 4554 5710 1790 Size: 1777 987 1156 2573 Fragments arranged by size: 2573 1777 1156 987 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3016 3694 4224 Size: 416 53 83 399 24 1599 678 530 Cuts at: 4224 5182 442 Size: 958 1753 Fragments arranged by size: 1753 1599 958 678 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3317 3317 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 2781 3287 3365 5153 1239 Size: 1280 262 506 78 1788 2579 Fragments arranged by size: 2579 1788 1280 506 262 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4618 5483 5781 4618 Size: 865 298 5330 Fragments arranged by size: 5330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 2451 6487 2451 Size: 4036 2457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4300 4682 5317 275 Size: 53 83 186 936 2767 382 635 1451 Fragments arranged by size: 2767 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4926 5324 5598 5745 6156 4926 Size: 398 274 147 411 5263 Fragments arranged by size: 5263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3131 3161 1178 Size: 30 165 30 1728 30 4510 Fragments arranged by size: 4510 1728 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 2897 3554 3590 1186 Size: 1246 465 657 36 4089 Fragments arranged by size: 4089 1246 657 465 36 BseYI C'CCAG_C Cuts at: 3504 5696 6192 3504 Size: 2192 496 3805 Fragments arranged by size: 3805 2192 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5920 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 3943 4704 5732 1540 Size: 275 186 28 1914 761 1028 2301 Fragments arranged by size: 2301 1914 1028 761 275 186 28 BsiHKAI G_wGCw'C Cuts at: 2298 2889 2937 3324 4554 4639 5710 2298 Size: 591 48 387 1230 85 1071 3081 Fragments arranged by size: 3081 1230 1071 591 387 85 48 BsmI GAATG_Cn' Cuts at: 2480 2600 2769 2966 4004 4097 2480 Size: 120 169 197 1038 93 4876 Fragments arranged by size: 4876 1038 197 169 120 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2889 2937 3053 3264 3324 3567 Size: 399 508 591 48 116 211 60 243 Cuts at: 3567 3812 4554 4639 5710 1391 Size: 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 508 399 245 243 211 116 85 60 48 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3848 844 Size: 3004 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3487 3580 4380 1394 Size: 429 1664 93 800 3507 Fragments arranged by size: 3507 1664 800 429 93 BsrDI GCAATG_nn' Cuts at: 65 1248 3729 4988 5162 65 Size: 1183 2481 1259 174 1396 Fragments arranged by size: 2481 1396 1259 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2218 3353 5137 5324 1700 Size: 110 408 1135 1784 187 2869 Fragments arranged by size: 2869 1784 1135 408 187 110 BsrGI T'GTAC_A Cuts at: 96 3468 96 Size: 3372 3121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 3589 794 Size: 2795 3698 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 2504 1184 Size: 1320 5173 BstXI CCAn_nnnn'nTGG Cuts at: 2582 2582 Size: 6493 BstYI r'GATC_y Cuts at: 1972 2383 2399 2431 2452 2577 2898 3823 4190 Size: 411 16 32 21 125 321 925 367 Cuts at: 4190 4575 4592 6044 6130 6142 6488 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 925 411 385 367 346 321 125 86 32 21 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2463 2847 2994 3129 3450 513 Size: 552 939 459 384 147 135 321 3556 Fragments arranged by size: 3556 939 552 459 384 321 147 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2841 487 Size: 683 56 1615 4139 Fragments arranged by size: 4139 1615 683 56 BtsI GCAGTG_nn' Cuts at: 3587 4009 4803 4823 3587 Size: 422 794 20 5257 Fragments arranged by size: 5257 794 422 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2610 3940 Size: 54 1425 50 275 18 196 584 1330 Cuts at: 3940 4829 6458 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1330 889 584 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2026 3940 1537 Size: 275 214 1914 4090 Fragments arranged by size: 4090 1914 275 214 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 4423 1353 Size: 1274 1796 3423 Fragments arranged by size: 3423 1796 1274 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3544 5080 5454 5600 387 Size: 1064 138 1955 1536 374 146 1280 Fragments arranged by size: 1955 1536 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2887 2887 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2781 3126 3287 3365 4556 5153 Size: 393 887 262 345 161 78 1191 597 Cuts at: 5153 5940 6208 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1191 887 787 597 393 345 268 262 161 78 EcoNI CCTnn'n_nnAGG Cuts at: 3347 3347 Size: 6493 EcoO109I rG'GnC_Cy Cuts at: 2839 3544 3563 3564 2839 Size: 705 19 1 5768 Fragments arranged by size: 5768 705 19 1 EcoRI G'AATT_C Cuts at: 2656 2872 3802 4180 2656 Size: 216 930 378 4969 Fragments arranged by size: 4969 930 378 216 FseI GG_CCGG'CC Cuts at: 3357 3357 Size: 6493 FspI TGC'GCA Cuts at: 2095 4999 2095 Size: 2904 3589 HaeII r_GCGC'y Cuts at: 1678 5640 1678 Size: 3962 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 3131 3163 3757 Size: 32 135 32 872 32 655 32 594 Cuts at: 3757 3789 5134 5166 5208 5240 1373 Size: 32 1345 32 42 32 2626 Fragments arranged by size: 2626 1345 872 655 594 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 3617 3837 4083 669 Size: 988 281 1234 445 220 246 3079 Fragments arranged by size: 3079 1234 988 445 281 246 220 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 3072 4092 3072 Size: 1020 5473 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3684 3743 5607 5791 1305 Size: 2379 59 1864 184 2007 Fragments arranged by size: 2379 2007 1864 184 59 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 10 Size: 54 1425 343 780 3891 Fragments arranged by size: 3891 1425 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4451 4810 4969 518 Size: 648 3285 359 159 2042 Fragments arranged by size: 3285 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2225 2374 2734 2869 3508 3909 4586 5734 Size: 619 149 360 135 639 401 677 1148 Cuts at: 5734 5979 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 639 619 401 360 245 149 135 NaeI GCC'GGC Cuts at: 2220 3355 2220 Size: 1135 5358 NcoI C'CATG_G Cuts at: 513 1065 2004 2463 513 Size: 552 939 459 4543 Fragments arranged by size: 4543 939 552 459 NdeI CA'TA_TG Cuts at: 387 2740 387 Size: 2353 4140 NgoMIV G'CCGG_C Cuts at: 2218 3353 2218 Size: 1135 5358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NruI TCG'CGA Cuts at: 2067 2067 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 3037 3851 5396 1132 Size: 97 1808 814 1545 2229 Fragments arranged by size: 2229 1808 1545 814 97 PasI CC'CwG_GG Cuts at: 1348 3560 1348 Size: 2212 4281 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 2447 1108 Size: 1339 5154 PfoI T'CCnGG_A Cuts at: 2082 2082 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2839 3544 2839 Size: 705 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 3563 3563 Size: 6493 PstI C_TGCA'G Cuts at: 830 2868 2881 2965 3606 3845 830 Size: 2038 13 84 641 239 3478 Fragments arranged by size: 3478 2038 641 239 84 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2225 2734 2869 3508 2225 Size: 509 135 639 5210 Fragments arranged by size: 5210 639 509 135 SacI G_AGCT'C Cuts at: 2889 2889 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SfcI C'TryA_G Cuts at: 826 1045 2864 2877 2961 3602 3841 4976 5657 Size: 219 1819 13 84 641 239 1135 681 Cuts at: 5657 5848 826 Size: 191 1471 Fragments arranged by size: 1819 1471 1135 681 641 239 219 191 84 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4597 5498 5760 820 Size: 197 926 2654 901 262 1553 Fragments arranged by size: 2654 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 3229 152 Size: 3077 3416 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SspI AAT'ATT Cuts at: 5 52 2664 4417 5 Size: 47 2612 1753 2081 Fragments arranged by size: 2612 2081 1753 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6493 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 2532 3652 3932 513 Size: 552 939 459 69 1120 280 3074 Fragments arranged by size: 3074 1120 939 552 459 280 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2378 4520 4537 4690 4875 5320 325 Size: 1255 798 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 798 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3468 4739 Size: 274 80 33 51 1443 444 1047 1271 Cuts at: 4739 6451 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1271 1047 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3707 3707 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI