(Circular) (Six-base) MAPSORT of: pFN21AB0496.seq Check: 4237 from: 1 to: 7831 pFN21AB0496 7831 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3053 3053 Size: 7831 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5641 278 Size: 53 83 186 936 4105 2468 Fragments arranged by size: 4105 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4460 5174 1937 Size: 2523 714 4594 Fragments arranged by size: 4594 2523 714 Acc65I G'GTAC_C Cuts at: 5152 5152 Size: 7831 AclI AA'CG_TT Cuts at: 1563 2143 4309 5958 6331 1563 Size: 580 2166 1649 373 3063 Fragments arranged by size: 3063 2166 1649 580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3001 3441 4541 5894 7278 7546 1632 Size: 654 715 440 1100 1353 1384 268 1917 Fragments arranged by size: 1917 1384 1353 1100 715 654 440 268 AflII C'TTAA_G Cuts at: 820 1017 2272 2539 3659 820 Size: 197 1255 267 1120 4992 Fragments arranged by size: 4992 1255 1120 267 197 AflIII A'CryG_T Cuts at: 1203 3331 3727 3753 4570 6730 7707 1203 Size: 2128 396 26 817 2160 977 1327 Fragments arranged by size: 2160 2128 1327 977 817 396 26 AgeI A'CCGG_T Cuts at: 6662 6662 Size: 7831 AhdI GACnn_n'nnGTC Cuts at: 6560 6560 Size: 7831 AleI CACnn'nnGTG Cuts at: 1166 3372 1166 Size: 2206 5625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3307 4833 1184 Size: 2123 1526 4182 Fragments arranged by size: 4182 2123 1526 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2399 Size: 32 7799 AlwNI CAG_nnn'CTG Cuts at: 2123 4145 5574 7146 2123 Size: 2022 1429 1572 2808 Fragments arranged by size: 2808 2022 1572 1429 ApaLI G'TGCA_C Cuts at: 5888 7044 5888 Size: 1156 6675 ApoI r'AATT_y Cuts at: 1493 2618 2763 3157 3408 3512 3823 4030 4129 Size: 1125 145 394 251 104 311 207 99 Cuts at: 4129 4444 4753 5069 5140 5366 5518 7627 1493 Size: 315 309 316 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 1125 394 316 315 311 309 251 226 207 152 145 104 99 71 AseI AT'TA_AT Cuts at: 160 1032 2658 3741 6385 160 Size: 872 1626 1083 2644 1606 Fragments arranged by size: 2644 1626 1606 1083 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 AvaI C'yCGr_G Cuts at: 1870 1943 5156 1870 Size: 73 3213 4545 Fragments arranged by size: 4545 3213 73 BamHI G'GATC_C Cuts at: 4075 5161 5528 4075 Size: 1086 367 6378 Fragments arranged by size: 6378 1086 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4998 5152 6607 618 Size: 325 217 627 3211 154 1455 1842 Fragments arranged by size: 3211 1842 1455 627 325 217 154 BanII G_rGCy'C Cuts at: 1391 3638 3668 4662 5150 1391 Size: 2247 30 994 488 4072 Fragments arranged by size: 4072 2247 994 488 30 BbeI G_GCGC'C Cuts at: 5002 5002 Size: 7831 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 928 Size: 971 375 6485 Fragments arranged by size: 6485 971 375 BbvCI CC'TCA_GC Cuts at: 2494 2494 Size: 7831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3035 3069 6022 6056 1903 Size: 34 1098 34 2953 34 3678 Fragments arranged by size: 3678 2953 1098 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5723 6933 5723 Size: 1210 6621 BclI T'GATC_A Cuts at: 1548 1548 Size: 7831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6442 136 Size: 107 122 71 631 605 4770 1525 Fragments arranged by size: 4770 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7826 7826 Size: 7831 BlpI GC'TnA_GC Cuts at: 5248 5248 Size: 7831 Bme1580I G_kGCm'C Cuts at: 1790 5892 7048 1790 Size: 4102 1156 2573 Fragments arranged by size: 4102 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4187 5562 6520 Size: 416 53 83 399 24 2770 1375 958 Cuts at: 6520 442 Size: 1753 Fragments arranged by size: 2770 1753 1375 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7831 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2068 Size: 7831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 4950 6491 1239 Size: 861 2850 1541 2579 Fragments arranged by size: 2850 2579 1541 861 Bpu10I CC'TnA_GC Cuts at: 2403 2494 2403 Size: 91 7740 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5956 6821 7119 5956 Size: 865 298 6668 Fragments arranged by size: 6668 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3075 3446 6494 882 Size: 617 1576 371 3048 2219 Fragments arranged by size: 3048 2219 1576 617 371 BsaAI yAC'GTr Cuts at: 493 1204 3728 5622 493 Size: 711 2524 1894 2702 Fragments arranged by size: 2702 2524 1894 711 BsaBI GATnn'nnATC Cuts at: 7825 7825 Size: 7831 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4999 5638 6020 6655 Size: 53 83 186 936 3466 639 382 635 Cuts at: 6655 275 Size: 1451 Fragments arranged by size: 3466 1451 936 639 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6264 6662 6936 7083 7494 6264 Size: 398 274 147 411 6601 Fragments arranged by size: 6601 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4011 4041 1178 Size: 30 165 30 2608 30 4968 Fragments arranged by size: 4968 2608 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7831 BseYI C'CCAG_C Cuts at: 4014 7034 7530 4014 Size: 3020 496 4315 Fragments arranged by size: 4315 3020 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2469 1163 Size: 573 733 6525 Fragments arranged by size: 6525 733 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4259 5281 6042 7070 1540 Size: 275 186 2258 1022 761 1028 2301 Fragments arranged by size: 2301 2258 1028 1022 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4662 5892 5977 7048 4662 Size: 1230 85 1071 5445 Fragments arranged by size: 5445 1230 1071 85 BsmI GAATG_Cn' Cuts at: 2015 4356 5342 5435 2015 Size: 2341 986 93 4411 Fragments arranged by size: 4411 2341 986 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3638 3668 4662 5150 5892 5977 7048 Size: 399 1848 30 994 488 742 85 1071 Cuts at: 7048 1391 Size: 2174 Fragments arranged by size: 2174 1848 1071 994 742 488 399 85 30 BspEI T'CCGG_A Cuts at: 7494 7494 Size: 7831 BspHI T'CATG_A Cuts at: 5720 7450 5720 Size: 1730 6101 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 4174 5186 844 Size: 2209 1121 1012 3489 Fragments arranged by size: 3489 2209 1121 1012 BsrBI CCG'CTC Cuts at: 1394 1823 5718 1394 Size: 429 3895 3507 Fragments arranged by size: 3895 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2526 2948 3048 6326 6500 65 Size: 1183 1278 422 100 3278 174 1396 Fragments arranged by size: 3278 1396 1278 1183 422 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 4258 6475 6662 1700 Size: 110 2448 2217 187 2869 Fragments arranged by size: 2869 2448 2217 187 110 BsrGI T'GTAC_A Cuts at: 96 2333 96 Size: 2237 5594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7831 BssSI C'ACGA_G Cuts at: 2385 5891 6903 2385 Size: 3506 1012 3313 Fragments arranged by size: 3506 3313 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 4487 794 Size: 1560 2133 4138 Fragments arranged by size: 4138 2133 1560 BstBI TT'CG_AA Cuts at: 2208 6635 2208 Size: 4427 3404 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7831 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 7831 BstYI r'GATC_y Cuts at: 1972 4075 5161 5528 5913 5930 7382 7468 7480 Size: 2103 1086 367 385 17 1452 86 12 Cuts at: 7480 7826 1972 Size: 346 1977 Fragments arranged by size: 2103 1977 1452 1086 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4461 4461 Size: 7831 Bsu36I CC'TnA_GG Cuts at: 3321 3321 Size: 7831 BtgI C'CryG_G Cuts at: 513 1065 2004 3370 3447 513 Size: 552 939 1366 77 4897 Fragments arranged by size: 4897 1366 939 552 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7092 Fragments arranged by size: 7092 683 56 BtsI GCAGTG_nn' Cuts at: 2058 4231 4654 5347 6141 6161 2058 Size: 2173 423 693 794 20 3728 Fragments arranged by size: 3728 2173 794 693 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7796 DraI TTT'AAA Cuts at: 4284 4891 5135 4284 Size: 607 244 6980 Fragments arranged by size: 6980 607 244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4800 5025 6838 809 Size: 507 3484 225 1813 1802 Fragments arranged by size: 3484 1813 1802 507 225 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3072 4256 5278 Size: 54 1425 50 275 18 1242 1184 1022 Cuts at: 5278 6167 7796 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1242 1184 1022 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4256 5278 1537 Size: 275 2444 1022 4090 Fragments arranged by size: 4090 2444 1022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 2355 3344 5761 1353 Size: 894 108 989 2417 3423 Fragments arranged by size: 3423 2417 989 894 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3109 6418 6792 6938 387 Size: 1064 138 435 1085 3309 374 146 1280 Fragments arranged by size: 3309 1280 1085 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 4660 4660 Size: 7831 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2286 3001 3441 4541 4950 5894 Size: 393 468 186 715 440 1100 409 944 Cuts at: 5894 6491 7278 7546 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1100 944 787 715 597 468 440 409 393 268 186 EcoO109I rG'GnC_Cy Cuts at: 2111 2111 Size: 7831 EcoRI G'AATT_C Cuts at: 3823 4753 5140 5518 3823 Size: 930 387 378 6136 Fragments arranged by size: 6136 930 387 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5030 5030 Size: 7831 FspI TGC'GCA Cuts at: 6337 6337 Size: 7831 HaeII r_GCGC'y Cuts at: 1678 5002 6978 1678 Size: 3324 1976 2531 Fragments arranged by size: 3324 2531 1976 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3281 3313 3553 3585 4487 Size: 32 135 32 1709 32 240 32 902 Cuts at: 4487 4519 6472 6504 6546 6578 1373 Size: 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 1709 902 240 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 5175 5421 669 Size: 988 281 520 2717 246 3079 Fragments arranged by size: 3079 2717 988 520 281 246 HindIII A'AGCT_T Cuts at: 748 4398 748 Size: 3650 4181 HpaI GTT'AAC Cuts at: 5421 5421 Size: 7831 KasI G'GCGC_C Cuts at: 4998 4998 Size: 7831 KpnI G_GTAC'C Cuts at: 5156 5156 Size: 7831 MfeI C'AATT_G Cuts at: 3285 3642 5430 3285 Size: 357 1788 5686 Fragments arranged by size: 5686 1788 357 MluI A'CGCG_T Cuts at: 7707 7707 Size: 7831 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2371 3187 3738 6945 7129 1305 Size: 1066 816 551 3207 184 2007 Fragments arranged by size: 3207 2007 1066 816 551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3074 10 Size: 54 1425 343 1242 4767 Fragments arranged by size: 4767 1425 1242 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3372 3560 5789 6148 6307 518 Size: 648 2206 188 2229 359 159 2042 Fragments arranged by size: 2229 2206 2042 648 359 188 159 MspA1I CmG'CkG Cuts at: 1606 2498 3671 5247 5924 7072 7317 1606 Size: 892 1173 1576 677 1148 245 2120 Fragments arranged by size: 2120 1576 1173 1148 892 677 245 NarI GG'CG_CC Cuts at: 4999 4999 Size: 7831 NcoI C'CATG_G Cuts at: 513 1065 2004 3370 513 Size: 552 939 1366 4974 Fragments arranged by size: 4974 1366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7831 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7831 NotI GC'GGCC_GC Cuts at: 5278 5278 Size: 7831 NspI r_CATG'y Cuts at: 1132 1229 3335 3478 3757 5189 6734 1132 Size: 97 2106 143 279 1432 1545 2229 Fragments arranged by size: 2229 2106 1545 1432 279 143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7831 PciI A'CATG_T Cuts at: 3331 3753 6730 3331 Size: 422 2977 4432 Fragments arranged by size: 4432 2977 422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2390 3644 1108 Size: 1282 1254 5295 Fragments arranged by size: 5295 1282 1254 PfoI T'CCnGG_A Cuts at: 3799 3799 Size: 7831 PmeI GTTT'AAAC Cuts at: 5135 5135 Size: 7831 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5900 5932 7413 7445 5900 Size: 32 1481 32 6286 Fragments arranged by size: 6286 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4964 1613 Size: 3351 4480 PsiI TTA'TAA Cuts at: 3885 5401 3885 Size: 1516 6315 PstI C_TGCA'G Cuts at: 830 2128 2359 4711 5183 830 Size: 1298 231 2352 472 3478 Fragments arranged by size: 3478 2352 1298 472 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7831 SacI G_AGCT'C Cuts at: 4662 4662 Size: 7831 SalI G'TCGA_C Cuts at: 1936 5173 1936 Size: 3237 4594 SbfI CC_TGCA'GG Cuts at: 5183 5183 Size: 7831 ScaI AGT'ACT Cuts at: 4098 6079 7791 4098 Size: 1981 1712 4138 Fragments arranged by size: 4138 1981 1712 SexAI A'CCwGG_T Cuts at: 3970 3970 Size: 7831 SfcI C'TryA_G Cuts at: 826 1045 2124 2355 3402 4108 4137 4707 5179 Size: 219 1079 231 1047 706 29 570 472 Cuts at: 5179 6314 6995 7186 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1079 1047 706 681 570 472 231 219 191 29 SfoI GGC'GCC Cuts at: 5000 5000 Size: 7831 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7831 SmaI CCC'GGG Cuts at: 1872 5158 1872 Size: 3286 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2272 2539 3659 5935 6836 7098 Size: 197 926 329 267 1120 2276 901 262 Cuts at: 7098 820 Size: 1553 Fragments arranged by size: 2276 1553 1120 926 901 329 267 262 197 SnaBI TAC'GTA Cuts at: 493 5622 493 Size: 5129 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7831 SphI G_CATG'C Cuts at: 1132 5189 1132 Size: 4057 3774 SspI AAT'ATT Cuts at: 5 52 2562 5755 5 Size: 47 2510 3193 2081 Fragments arranged by size: 3193 2510 2081 47 StuI AGG'CCT Cuts at: 1517 3437 1517 Size: 1920 5911 StyI C'CwwG_G Cuts at: 513 1065 2004 3370 5270 513 Size: 552 939 1366 1900 3074 Fragments arranged by size: 3074 1900 1366 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5858 5875 6028 6213 6658 325 Size: 1255 4278 17 153 185 445 1498 Fragments arranged by size: 4278 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2333 3334 3750 Size: 274 80 33 51 1443 356 1001 416 Cuts at: 3750 4096 6077 7789 96 Size: 346 1981 1712 138 Fragments arranged by size: 1981 1712 1443 1001 416 356 346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4011 4043 7791 7823 4011 Size: 32 3748 32 4019 Fragments arranged by size: 4019 3748 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7831 XbaI T'CTAG_A Cuts at: 5167 5167 Size: 7831 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5125 5125 Size: 7831 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7831 XmaI C'CCGG_G Cuts at: 1870 5156 1870 Size: 3286 4545 XmnI GAAnn'nnTTC Cuts at: 2619 3527 3555 3933 4098 4417 5013 5960 2619 Size: 908 28 378 165 319 596 947 4490 Fragments arranged by size: 4490 947 908 596 378 319 165 28 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5639 276 Size: 53 83 186 936 4105 2468 Fragments arranged by size: 4105 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI