(Circular) (Six-base) MAPSORT of: pFN21AB6143.seq Check: 1417 from: 1 to: 6490 pFN21AB6143 6490 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3833 1937 Size: 1896 4594 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2981 4553 5937 6205 1632 Size: 1349 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 1349 268 AfeI AGC'GCT Cuts at: 3281 3281 Size: 6490 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 3642 5389 6366 1203 Size: 2439 1747 977 1327 Fragments arranged by size: 2439 1747 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 2326 5219 2326 Size: 2893 3597 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 2228 2480 4233 5805 2228 Size: 252 1753 1572 2913 Fragments arranged by size: 2913 1753 1572 252 ApaI G_GGCC'C Cuts at: 2386 2520 3293 2386 Size: 134 773 5583 Fragments arranged by size: 5583 773 134 ApaLI G'TGCA_C Cuts at: 4547 5703 4547 Size: 1156 5334 ApoI r'AATT_y Cuts at: 1493 2178 2218 2610 2624 2697 2969 3067 3799 Size: 685 40 392 14 73 272 98 732 Cuts at: 3799 4025 4177 6286 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 732 685 392 272 226 152 98 73 40 14 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2019 3815 1870 Size: 73 76 1796 4545 Fragments arranged by size: 4545 1796 76 73 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3163 3811 5266 618 Size: 325 217 627 1376 648 1455 1842 Fragments arranged by size: 1842 1455 1376 648 627 325 217 BanII G_rGCy'C Cuts at: 1391 2386 2407 2520 3293 3809 1391 Size: 995 21 113 773 516 4072 Fragments arranged by size: 4072 995 773 516 113 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2511 928 Size: 971 612 4907 Fragments arranged by size: 4907 971 612 BbvCI CC'TCA_GC Cuts at: 3758 3770 3758 Size: 12 6478 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3728 3762 4681 4715 1903 Size: 34 1791 34 919 34 3678 Fragments arranged by size: 3678 1791 919 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 5101 136 Size: 107 122 71 631 605 343 3086 1525 Fragments arranged by size: 3086 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 3379 3457 6485 3379 Size: 78 3028 3384 Fragments arranged by size: 3384 3028 78 BlpI GC'TnA_GC Cuts at: 2571 3907 2571 Size: 1336 5154 Bme1580I G_kGCm'C Cuts at: 1790 2386 2520 3293 4551 5707 1790 Size: 596 134 773 1258 1156 2573 Fragments arranged by size: 2573 1258 1156 773 596 134 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2181 2368 2443 Size: 416 53 83 399 24 764 187 75 Cuts at: 2443 2506 2518 4221 5179 442 Size: 63 12 1703 958 1753 Fragments arranged by size: 1753 1703 958 764 416 399 187 83 75 63 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 2421 3737 5150 1239 Size: 1117 65 1316 1413 2579 Fragments arranged by size: 2579 1413 1316 1117 65 Bpu10I CC'TnA_GC Cuts at: 2268 3758 3770 2268 Size: 1490 12 4988 Fragments arranged by size: 4988 1490 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4615 5480 5778 4615 Size: 865 298 5327 Fragments arranged by size: 5327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4281 493 Size: 711 3077 2702 Fragments arranged by size: 3077 2702 711 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2101 3678 4923 5321 5595 5742 6153 2101 Size: 1577 1245 398 274 147 411 2438 Fragments arranged by size: 2438 1577 1245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2407 2437 1178 Size: 30 165 30 1004 30 5231 Fragments arranged by size: 5231 1004 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6490 BseYI C'CCAG_C Cuts at: 2376 2385 5693 6189 2376 Size: 9 3308 496 2677 Fragments arranged by size: 3308 2677 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3268 1163 Size: 573 1532 4385 Fragments arranged by size: 4385 1532 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 4551 4636 5707 3273 Size: 1278 85 1071 4056 Fragments arranged by size: 4056 1278 1071 85 BsmI GAATG_Cn' Cuts at: 4001 4094 4001 Size: 93 6397 BsmBI CGTCTCn'nnnn_ Cuts at: 2897 2897 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2386 2407 2520 3273 3293 3809 4551 Size: 399 596 21 113 753 20 516 742 Cuts at: 4551 4636 5707 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 596 516 399 113 85 21 20 BspEI T'CCGG_A Cuts at: 2101 6153 2101 Size: 4052 2438 BspHI T'CATG_A Cuts at: 3135 4379 6109 3135 Size: 1244 1730 3516 Fragments arranged by size: 3516 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3845 844 Size: 3001 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2323 4985 5159 65 Size: 1183 1075 2662 174 1396 Fragments arranged by size: 2662 1396 1183 1075 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 3434 3485 4550 5562 3434 Size: 51 1065 1012 4362 Fragments arranged by size: 4362 1065 1012 51 BstAPI GCAn_nnn'nTGC Cuts at: 794 3727 794 Size: 2933 3557 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstXI CCAn_nnnn'nTGG Cuts at: 3229 3436 3229 Size: 207 6283 BstYI r'GATC_y Cuts at: 1972 2770 3379 3457 3681 3820 4187 4572 4589 Size: 798 609 78 224 139 367 385 17 Cuts at: 4589 6041 6127 6139 6485 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 798 609 385 367 346 224 139 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3150 3228 513 Size: 552 939 1146 78 3775 Fragments arranged by size: 3775 1146 939 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2317 2439 4006 4800 4820 2317 Size: 122 1567 794 20 3987 Fragments arranged by size: 3987 1567 794 122 20 ClaI AT'CG_AT Cuts at: 1554 2845 1554 Size: 1291 5199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2945 5497 809 Size: 507 1629 2552 1802 Fragments arranged by size: 2552 1802 1629 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2667 Size: 54 1425 50 275 18 177 442 218 Cuts at: 2667 3339 3937 4826 6455 8 Size: 672 598 889 1629 43 Fragments arranged by size: 1629 1425 889 672 598 442 275 218 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2533 2937 2958 3614 4420 1353 Size: 1180 404 21 656 806 3423 Fragments arranged by size: 3423 1180 806 656 404 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3695 5077 5451 5597 387 Size: 1064 138 2106 1382 374 146 1280 Fragments arranged by size: 2106 1382 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2421 2981 3737 4553 5150 5937 Size: 393 724 65 560 756 816 597 787 Cuts at: 5937 6205 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 756 724 597 560 393 268 65 EcoO109I rG'GnC_Cy Cuts at: 2382 3766 2382 Size: 1384 5106 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2258 3518 2258 Size: 1260 5230 FspI TGC'GCA Cuts at: 2984 4996 2984 Size: 2012 4478 FspAI rTGC'GCAy Cuts at: 2984 2984 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2571 3283 5637 1678 Size: 893 712 2354 2531 Fragments arranged by size: 2531 2354 893 712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2237 2269 2312 Size: 32 135 32 498 32 135 32 43 Cuts at: 2312 2344 2534 2566 3615 3647 5131 5163 5205 Size: 32 190 32 1049 32 1484 32 42 Cuts at: 5205 5237 1373 Size: 32 2626 Fragments arranged by size: 2626 1484 1049 498 190 135 135 43 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6490 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 3642 6366 3642 Size: 2724 3766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2692 5604 5788 1305 Size: 1387 2912 184 2007 Fragments arranged by size: 2912 2007 1387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2669 3341 10 Size: 54 1425 343 177 660 672 3159 Fragments arranged by size: 3159 1425 672 660 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4448 4807 4966 518 Size: 648 3282 359 159 2042 Fragments arranged by size: 3282 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2555 3906 4583 5731 5976 1606 Size: 949 1351 677 1148 245 2120 Fragments arranged by size: 2120 1351 1148 949 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3150 3228 513 Size: 552 939 1146 78 3775 Fragments arranged by size: 3775 1146 939 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 3848 5393 1132 Size: 97 2619 1545 2229 Fragments arranged by size: 2619 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2433 2480 1108 Size: 1325 47 5118 Fragments arranged by size: 5118 1325 47 PfoI T'CCnGG_A Cuts at: 2629 2629 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2142 2174 4559 4591 6072 6104 2142 Size: 32 2385 32 1481 32 2528 Fragments arranged by size: 2528 2385 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PspOMI G'GGCC_C Cuts at: 2382 2516 3289 2382 Size: 134 773 5583 Fragments arranged by size: 5583 773 134 PstI C_TGCA'G Cuts at: 830 3842 830 Size: 3012 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2555 2555 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SexAI A'CCwGG_T Cuts at: 2301 2301 Size: 6490 SfcI C'TryA_G Cuts at: 826 1045 3838 4973 5654 5845 826 Size: 219 2793 1135 681 191 1471 Fragments arranged by size: 2793 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4594 5495 5757 820 Size: 197 926 2651 901 262 1553 Fragments arranged by size: 2651 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 3571 152 Size: 3419 3071 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 2462 3002 3326 1517 Size: 945 540 324 4681 Fragments arranged by size: 4681 945 540 324 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2467 3150 3222 3228 3300 Size: 552 939 9 454 683 72 6 72 Cuts at: 3300 3929 513 Size: 629 3074 Fragments arranged by size: 3074 939 683 629 552 454 72 72 9 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4736 6448 96 Size: 274 80 33 51 1443 2759 1712 138 Fragments arranged by size: 2759 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2208 2240 2407 2439 6450 6482 2208 Size: 32 167 32 4011 32 2216 Fragments arranged by size: 4011 2216 167 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2309 2309 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 4619 4619 Size: 6490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI