Restriction map for
pFN21AB0479
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 1037 TCCGGA T^CCGGA
AfaI 936 GTAC GT^AC
AspHI 1231 GAGCTC G[AT]GC[AT]^C
BcgI 94 CGATGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 354 GGATAC GTATCC(6/5)
BglI 621 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 933 CACGTA [CT]AC^GT[GA]
BseAI 1037 TCCGGA T^CCGGA
BsgI 1411 CTGCAC GTGCAG(16/14)
BsiEI 631 CGGCCG CG[GA][CT]^CG
BsiHKAI 1231 GAGCTC G[AT]GC[AT]^C
BspEI 1037 TCCGGA T^CCGGA
BspHI 1421 TCATGA T^CATGA
BspMI 203 ACCTGC ACCTGC(4/8)
BsrDI 1280 GCAATG GCAATG(2/0)
BsrFI 1276 ACCGGC [GA]^CCGG[CT]
BstXI 1130 CCAAGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1328 AGATCC [GA]^GATC[CT]
Cfr10I 1276 ACCGGC [GA]^CCGG[CT]
DraIII 380 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 631 CGGCCG C^GGCCG
EarI 889 CTCTTC CTCTTC(1/4)
EclXI 631 CGGCCG C^GGCCG
Eco52I 631 CGGCCG C^GGCCG
HgaI 445 GACGC GACGC(5/10)
Ksp632I 889 CTCTTC CTCTTC(1/4)
KspI 330 CCGCGG CCGC^GG
MaeIII 1096 GTCAC ^GT[ACGT]AC
MflI 1328 AGATCC [GA]^GATC[CT]
MroI 1037 TCCGGA T^CCGGA
MslI 693 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 493 GCATGT [GA]CATG^[CT]
PaeR7I 314 CTCGAG C^TCGAG
PshAI 270 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1421 TCATGA T^CATGA
RsaI 936 GTAC GT^AC
SacI 1231 GAGCTC GAGCT^C
SacII 330 CCGCGG CCGC^GG
SapI 888 GCTCTTC GCTCTTC(1/4)
SbfI 1401 CCTGCAGG CCTGCA^GG
SexAI 381 ACCAGGT A^CC[AT]GGT
Sse8387I 1401 CCTGCAGG CCTGCA^GG
Tsp45I 1096 GTCAC ^GT[GC]AC
XcmI 996 CCACATCTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 314 CTCGAG C^TCGAG
XhoII 1328 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 638 GGGCCC GGGCC^C
877 GGGCCC
BbeI 866 GGCGCC GGCGC^C
1513 GGCGCC
BsaI 1126 GAGACC GGTCTC(1/5)
189 GGTCTC
BsaWI 1037 TCCGGA [AT]^CCGG[AT]
1331 TCCGGT
BseRI 1165 GAGGAG GAGGAG(10/8)
1384 GAGGAG
BsmAI 1126 GAGAC GTCTC(1/5)
190 GTCTC
BstAPI 198 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1404 GCAGGAGCTGC
HinfI 1151 GATTC G^A[ACGT]TC
1171 GAATC
KasI 866 GGCGCC G^GCGCC
1513 GGCGCC
MaeII 538 ACGT A^CGT
934 ACGT
MboII 1326 GAAGA GAAGA(8/7)
890 TCTTC
NarI 866 GGCGCC GG^CGCC
1513 GGCGCC
PpuMI 53 GGGTCCC [GA]G^G[AT]CC[CT]
434 GGGACCT
SfoI 866 GGCGCC GGC^GCC
1513 GGCGCC
SmaI 650 CCCGGG CCC^GGG
1517 CCCGGG
TfiI 1151 GATTC G^A[AT]TC
1171 GAATC
XmaCI 650 CCCGGG C^CCGGG
1517 CCCGGG
XmaI 650 CCCGGG C^CCGGG
1517 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI TspRI TthHB8I Van91I