(Circular) (Six-base) MAPSORT of: pFN21AB0479.seq Check: 3364 from: 1 to: 6259 pFN21AB0479 6259 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2217 2217 Size: 6259 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3602 1937 Size: 1665 4594 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 2671 2951 4322 5706 5974 1632 Size: 914 125 280 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 914 280 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 5158 6135 1203 Size: 3955 977 1327 Fragments arranged by size: 3955 1327 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 2702 1166 Size: 1536 4723 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 2759 3242 1184 Size: 1206 369 483 4201 Fragments arranged by size: 4201 1206 483 369 AlwNI CAG_nnn'CTG Cuts at: 2209 2393 2479 2929 3415 3538 4002 5574 2209 Size: 184 86 450 486 123 464 1572 2894 Fragments arranged by size: 2894 1572 486 464 450 184 123 86 ApaI G_GGCC'C Cuts at: 2647 2886 2647 Size: 239 6020 ApaLI G'TGCA_C Cuts at: 4316 5472 4316 Size: 1156 5103 ApoI r'AATT_y Cuts at: 1493 3568 3794 3946 6055 1493 Size: 2075 226 152 2109 1697 Fragments arranged by size: 2109 2075 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2655 3506 3522 3584 1870 Size: 73 376 336 851 16 62 4545 Fragments arranged by size: 4545 851 376 336 73 62 16 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2713 2773 2871 3518 3580 Size: 325 217 627 926 60 98 647 62 Cuts at: 3580 5035 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 926 647 627 325 217 98 62 60 BanII G_rGCy'C Cuts at: 1391 2647 2886 3240 3578 1391 Size: 1256 239 354 338 4072 Fragments arranged by size: 4072 1256 354 338 239 BbeI G_GCGC'C Cuts at: 2875 3522 2875 Size: 647 5612 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2088 2122 4450 4484 1903 Size: 34 151 34 2328 34 3678 Fragments arranged by size: 3678 2328 151 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2353 4151 5361 2353 Size: 1798 1210 3251 Fragments arranged by size: 3251 1798 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2632 4870 136 Size: 107 122 71 631 605 960 2238 1525 Fragments arranged by size: 2238 1525 960 631 605 122 107 71 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 2012 2647 2845 2886 3023 4320 5476 1790 Size: 222 635 198 41 137 1297 1156 2573 Fragments arranged by size: 2573 1297 1156 635 222 198 137 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 2057 3990 Size: 416 53 83 399 24 615 25 1933 Cuts at: 3990 4948 442 Size: 958 1753 Fragments arranged by size: 1933 1753 958 615 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2729 3398 3560 4919 1239 Size: 1100 390 669 162 1359 2579 Fragments arranged by size: 2579 1359 1100 669 390 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2182 2250 4384 5249 5547 2182 Size: 68 2134 865 298 2894 Fragments arranged by size: 2894 2134 865 298 68 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2200 3125 4922 882 Size: 617 701 925 1797 2219 Fragments arranged by size: 2219 1797 925 701 617 BsaAI yAC'GTr Cuts at: 493 1204 2940 4050 493 Size: 711 1736 1110 2702 Fragments arranged by size: 2702 1736 1110 711 BsaBI GATnn'nnATC Cuts at: 6253 6253 Size: 6259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 2872 3519 4066 Size: 53 83 186 936 918 421 647 547 Cuts at: 4066 4448 5083 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 918 647 635 547 421 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3042 3336 4692 5090 5364 5511 5922 3042 Size: 294 1356 398 274 147 411 3379 Fragments arranged by size: 3379 1356 411 398 294 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 3122 3152 3440 Size: 30 165 30 1002 30 687 30 288 Cuts at: 3440 3470 1178 Size: 30 3967 Fragments arranged by size: 3967 1002 687 288 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3185 3404 1186 Size: 1999 219 4041 Fragments arranged by size: 4041 1999 219 BseYI C'CCAG_C Cuts at: 2122 2166 2567 3204 5462 5958 2122 Size: 44 401 637 2258 496 2423 Fragments arranged by size: 2423 2258 637 496 401 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3401 1163 Size: 573 1665 4021 Fragments arranged by size: 4021 1665 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2639 3709 4470 5498 1540 Size: 275 186 638 1070 761 1028 2301 Fragments arranged by size: 2301 1070 1028 761 638 275 186 BsiHKAI G_wGCw'C Cuts at: 3240 4320 4405 5476 3240 Size: 1080 85 1071 4023 Fragments arranged by size: 4023 1080 1071 85 BsmI GAATG_Cn' Cuts at: 3770 3863 3770 Size: 93 6166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2647 2845 2886 3023 3240 3578 Size: 399 222 635 198 41 137 217 338 Cuts at: 3578 4320 4405 5476 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 635 399 338 222 217 198 137 85 41 BspEI T'CCGG_A Cuts at: 3042 5922 3042 Size: 2880 3379 BspHI T'CATG_A Cuts at: 3426 4148 5878 3426 Size: 722 1730 3807 Fragments arranged by size: 3807 1730 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 3614 844 Size: 1373 1397 3489 Fragments arranged by size: 3489 1397 1373 BsrBI CCG'CTC Cuts at: 1394 1823 2416 3030 3442 4146 1394 Size: 429 593 614 412 704 3507 Fragments arranged by size: 3507 704 614 593 429 412 BsrDI GCAATG_nn' Cuts at: 65 1248 3292 4754 4928 65 Size: 1183 2044 1462 174 1396 Fragments arranged by size: 2044 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3281 4903 5090 1700 Size: 110 1471 1622 187 2869 Fragments arranged by size: 2869 1622 1471 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6259 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 2209 3415 794 Size: 1415 1206 3638 Fragments arranged by size: 3638 1415 1206 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstXI CCAn_nnnn'nTGG Cuts at: 3142 3142 Size: 6259 BstYI r'GATC_y Cuts at: 1972 3333 3589 3956 4341 4358 5810 5896 5908 Size: 1361 256 367 385 17 1452 86 12 Cuts at: 5908 6254 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1361 385 367 346 256 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2277 2335 2509 2863 513 Size: 552 1212 58 174 354 3909 Fragments arranged by size: 3909 1212 552 354 174 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2442 487 Size: 683 56 1216 4304 Fragments arranged by size: 4304 1216 683 56 BtsI GCAGTG_nn' Cuts at: 2104 2219 2843 3775 4569 4589 2104 Size: 115 624 932 794 20 3774 Fragments arranged by size: 3774 932 794 624 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6224 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DraIII CAC_nnn'GTG Cuts at: 2390 2390 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2636 2751 3369 Size: 54 1425 50 275 18 806 115 618 Cuts at: 3369 3706 4595 6224 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1425 889 806 618 337 275 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2636 3706 1537 Size: 275 824 1070 4090 Fragments arranged by size: 4090 1070 824 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2900 4189 1353 Size: 1547 1289 3423 Fragments arranged by size: 3423 1547 1289 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2273 3448 4846 5220 5366 387 Size: 1064 138 684 1175 1398 374 146 1280 Fragments arranged by size: 1398 1280 1175 1064 684 374 146 138 EcoICRI GAG'CTC Cuts at: 3238 3238 Size: 6259 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2546 2671 2729 2951 3398 3560 Size: 393 707 207 125 58 222 447 162 Cuts at: 3560 4322 4919 5706 5974 1239 Size: 762 597 787 268 1524 Fragments arranged by size: 1524 787 762 707 597 447 393 268 222 207 162 125 58 EcoO109I rG'GnC_Cy Cuts at: 2059 2127 2440 2514 2625 2643 2883 3503 3535 Size: 68 313 74 111 18 240 620 32 Cuts at: 3535 2059 Size: 4783 Fragments arranged by size: 4783 620 313 240 111 74 68 32 18 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3040 3040 Size: 6259 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 2875 3191 3522 5406 1678 Size: 1197 316 331 1884 2531 Fragments arranged by size: 2531 1884 1197 331 316 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2405 2437 2441 2473 3161 Size: 32 135 32 833 32 4 32 688 Cuts at: 3161 3193 3440 3472 4900 4932 4974 5006 1373 Size: 32 247 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 833 688 247 135 42 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3603 3849 669 Size: 988 281 1665 246 3079 Fragments arranged by size: 3079 1665 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6259 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KasI G'GCGC_C Cuts at: 2871 3518 2871 Size: 647 5612 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2053 2427 5373 5557 1305 Size: 748 374 2946 184 2007 Fragments arranged by size: 2946 2007 748 374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4437 Fragments arranged by size: 4437 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 4217 4576 4735 518 Size: 648 1536 1515 359 159 2042 Fragments arranged by size: 2042 1536 1515 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2337 2749 2770 2986 3250 3368 3675 4352 Size: 731 412 21 216 264 118 307 677 Cuts at: 4352 5500 5745 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 731 677 412 307 264 245 216 118 21 NarI GG'CG_CC Cuts at: 2872 3519 2872 Size: 647 5612 NcoI C'CATG_G Cuts at: 513 1065 2277 2509 2863 513 Size: 552 1212 232 354 3909 Fragments arranged by size: 3909 1212 552 354 232 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 2502 3617 5162 1132 Size: 97 1273 1115 1545 2229 Fragments arranged by size: 2229 1545 1273 1115 97 PasI CC'CwG_GG Cuts at: 1348 2473 2629 1348 Size: 1125 156 4978 Fragments arranged by size: 4978 1125 156 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 2278 2479 2619 3538 1108 Size: 1170 201 140 919 3829 Fragments arranged by size: 3829 1170 919 201 140 PfoI T'CCnGG_A Cuts at: 2355 3117 3375 2355 Size: 762 258 5239 Fragments arranged by size: 5239 762 258 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4328 4360 5841 5873 4328 Size: 32 1481 32 4714 Fragments arranged by size: 4714 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2440 2059 Size: 381 5878 PshAI GACnn'nnGTC Cuts at: 1613 2279 1613 Size: 666 5593 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PspOMI G'GGCC_C Cuts at: 2643 2882 2643 Size: 239 6020 PstI C_TGCA'G Cuts at: 830 2748 2838 3411 3611 830 Size: 1918 90 573 200 3478 Fragments arranged by size: 3478 1918 573 200 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 2749 2986 3250 2749 Size: 237 264 5758 Fragments arranged by size: 5758 264 237 SacI G_AGCT'C Cuts at: 3240 3240 Size: 6259 SacII CC_GC'GG Cuts at: 2338 2338 Size: 6259 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SanDI GG'GwC_CC Cuts at: 2059 2059 Size: 6259 SapI GCTCTTCn'nnn_ Cuts at: 2900 2900 Size: 6259 SbfI CC_TGCA'GG Cuts at: 3411 3611 3411 Size: 200 6059 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SexAI A'CCwGG_T Cuts at: 2386 2386 Size: 6259 SfcI C'TryA_G Cuts at: 826 1045 2584 2744 2834 3407 3607 4742 5423 Size: 219 1539 160 90 573 200 1135 681 Cuts at: 5423 5614 826 Size: 191 1471 Fragments arranged by size: 1539 1471 1135 681 573 219 200 191 160 90 SfoI GGC'GCC Cuts at: 2873 3520 2873 Size: 647 5612 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 2657 3524 3586 1872 Size: 785 867 62 4545 Fragments arranged by size: 4545 867 785 62 SmlI C'TyrA_G Cuts at: 820 1017 1943 2197 2265 2319 4363 5264 5526 Size: 197 926 254 68 54 2044 901 262 Cuts at: 5526 820 Size: 1553 Fragments arranged by size: 2044 1553 926 901 262 254 197 68 54 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 3617 1132 Size: 2485 3774 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6259 StyI C'CwwG_G Cuts at: 513 1065 2277 2509 2613 2863 3698 513 Size: 552 1212 232 104 250 835 3074 Fragments arranged by size: 3074 1212 835 552 250 232 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 4286 4303 4456 4641 5086 325 Size: 1255 695 2011 17 153 185 445 1498 Fragments arranged by size: 2011 1498 1255 695 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4505 6217 96 Size: 274 80 33 51 1443 2528 1712 138 Fragments arranged by size: 2528 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6259 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3008 3008 Size: 6259 XhoI C'TCGA_G Cuts at: 1943 2319 1943 Size: 376 5883 XmaI C'CCGG_G Cuts at: 1870 2655 3522 3584 1870 Size: 785 867 62 4545 Fragments arranged by size: 4545 867 785 62 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI