Restriction map for
pFN21AB0479
(Linear) (Six-base) MAPSORT of: KIBB0479.seq Check: 3266 from: 1 to: 1554
KIBB0479.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 212 1554
Size: 212 1342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 666 946 1554
Size: 541 125 280 608
Fragments arranged by size:
608 541 280 125
AleI CACnn'nnGTG
Cuts at: 0 697 1554
Size: 697 857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 754 1237 1554
Size: 385 369 483 317
Fragments arranged by size:
483 385 369 317
AlwNI CAG_nnn'CTG
Cuts at: 0 204 388 474 924 1410 1533 1554
Size: 204 184 86 450 486 123 21
Fragments arranged by size:
486 450 204 184 123 86 21
ApaI G_GGCC'C
Cuts at: 0 642 881 1554
Size: 642 239 673
Fragments arranged by size:
673 642 239
AvaI C'yCGr_G
Cuts at: 0 314 650 1501 1517 1554
Size: 314 336 851 16 37
Fragments arranged by size:
851 336 314 37 16
BanI G'GyrC_C
Cuts at: 0 708 768 866 1513 1554
Size: 708 60 98 647 41
Fragments arranged by size:
708 647 98 60 41
BanII G_rGCy'C
Cuts at: 0 642 881 1235 1554
Size: 642 239 354 319
Fragments arranged by size:
642 354 319 239
BbeI G_GCGC'C
Cuts at: 0 870 1517 1554
Size: 870 647 37
Fragments arranged by size:
870 647 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 117 1554
Size: 83 34 1437
Fragments arranged by size:
1437 83 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 348 1554
Size: 348 1206
BglI GCCn_nnn'nGGC
Cuts at: 0 627 1554
Size: 627 927
Bme1580I G_kGCm'C
Cuts at: 0 7 642 840 881 1018 1554
Size: 7 635 198 41 137 536
Fragments arranged by size:
635 536 198 137 41 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 52 1554
Size: 27 25 1502
Fragments arranged by size:
1502 27 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 724 1393 1554
Size: 334 390 669 161
Fragments arranged by size:
669 390 334 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 245 1554
Size: 177 68 1309
Fragments arranged by size:
1309 177 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 195 1120 1554
Size: 195 925 434
Fragments arranged by size:
925 434 195
BsaAI yAC'GTr
Cuts at: 0 935 1554
Size: 935 619
BsaHI Gr'CG_yC
Cuts at: 0 446 867 1514 1554
Size: 446 421 647 40
Fragments arranged by size:
647 446 421 40
BsaWI w'CCGG_w
Cuts at: 0 1037 1331 1554
Size: 1037 294 223
Fragments arranged by size:
1037 294 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 1117 1147 1435 1465 1554
Size: 400 30 687 30 288 30 89
Fragments arranged by size:
687 400 288 89 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1180 1399 1554
Size: 1180 219 155
Fragments arranged by size:
1180 219 155
BseYI C'CCAG_C
Cuts at: 0 117 161 562 1199 1554
Size: 117 44 401 637 355
Fragments arranged by size:
637 401 355 117 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1554
Size: 1396 158
BsiEI CG_ry'CG
Cuts at: 0 634 1554
Size: 634 920
BsiHKAI G_wGCw'C
Cuts at: 0 1235 1554
Size: 1235 319
Bsp1286I G_dGCh'C
Cuts at: 0 7 642 840 881 1018 1235 1554
Size: 7 635 198 41 137 217 319
Fragments arranged by size:
635 319 217 198 137 41 7
BspEI T'CCGG_A
Cuts at: 0 1037 1554
Size: 1037 517
BspHI T'CATG_A
Cuts at: 0 1421 1554
Size: 1421 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 1554
Size: 212 1342
BsrBI CCG'CTC
Cuts at: 0 411 1025 1437 1554
Size: 411 614 412 117
Fragments arranged by size:
614 412 411 117
BsrDI GCAATG_nn'
Cuts at: 0 1287 1554
Size: 1287 267
BsrFI r'CCGG_y
Cuts at: 0 1276 1554
Size: 1276 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 1410 1554
Size: 204 1206 144
Fragments arranged by size:
1206 204 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1137 1554
Size: 1137 417
BstYI r'GATC_y
Cuts at: 0 1328 1554
Size: 1328 226
BtgI C'CryG_G
Cuts at: 0 272 330 504 858 1554
Size: 272 58 174 354 696
Fragments arranged by size:
696 354 272 174 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 437 1554
Size: 437 1117
BtsI GCAGTG_nn'
Cuts at: 0 99 214 838 1554
Size: 99 115 624 716
Fragments arranged by size:
716 624 115 99
DraIII CAC_nnn'GTG
Cuts at: 0 385 1554
Size: 385 1169
EaeI y'GGCC_r
Cuts at: 0 631 746 1364 1554
Size: 631 115 618 190
Fragments arranged by size:
631 618 190 115
EagI C'GGCC_G
Cuts at: 0 631 1554
Size: 631 923
EarI CTCTTCn'nnn_
Cuts at: 0 895 1554
Size: 895 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 268 1443 1554
Size: 268 1175 111
Fragments arranged by size:
1175 268 111
EcoICRI GAG'CTC
Cuts at: 0 1233 1554
Size: 1233 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 541 666 724 946 1393 1554
Size: 334 207 125 58 222 447 161
Fragments arranged by size:
447 334 222 207 161 125 58
EcoO109I rG'GnC_Cy
Cuts at: 0 54 122 435 509 620 638 878 1498
Size: 54 68 313 74 111 18 240 620
Cuts at: 1498 1530 1554
Size: 32 24
Fragments arranged by size:
620 313 240 111 74 68 54 32
24 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1035 1554
Size: 1035 519
HaeII r_GCGC'y
Cuts at: 0 870 1186 1517 1554
Size: 870 316 331 37
Fragments arranged by size:
870 331 316 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 436 468 1156 1188 1435 1467
Size: 400 32 4 32 688 32 247 32
Cuts at: 1467 1554
Size: 87
Fragments arranged by size:
688 400 247 87 32 32 32 32
4
KasI G'GCGC_C
Cuts at: 0 866 1513 1554
Size: 866 647 41
Fragments arranged by size:
866 647 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 422 1554
Size: 48 374 1132
Fragments arranged by size:
1132 374 48
MslI CAynn'nnrTG
Cuts at: 0 697 1554
Size: 697 857
MspA1I CmG'CkG
Cuts at: 0 332 744 765 981 1245 1363 1554
Size: 332 412 21 216 264 118 191
Fragments arranged by size:
412 332 264 216 191 118 21
NarI GG'CG_CC
Cuts at: 0 867 1514 1554
Size: 867 647 40
Fragments arranged by size:
867 647 40
NcoI C'CATG_G
Cuts at: 0 272 504 858 1554
Size: 272 232 354 696
Fragments arranged by size:
696 354 272 232
NspI r_CATG'y
Cuts at: 0 497 1554
Size: 497 1057
PasI CC'CwG_GG
Cuts at: 0 468 624 1554
Size: 468 156 930
Fragments arranged by size:
930 468 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 474 614 1533 1554
Size: 273 201 140 919 21
Fragments arranged by size:
919 273 201 140 21
PfoI T'CCnGG_A
Cuts at: 0 350 1112 1370 1554
Size: 350 762 258 184
Fragments arranged by size:
762 350 258 184
PpuMI rG'GwC_Cy
Cuts at: 0 54 435 1554
Size: 54 381 1119
Fragments arranged by size:
1119 381 54
PshAI GACnn'nnGTC
Cuts at: 0 274 1554
Size: 274 1280
PspOMI G'GGCC_C
Cuts at: 0 638 877 1554
Size: 638 239 677
Fragments arranged by size:
677 638 239
PstI C_TGCA'G
Cuts at: 0 743 833 1406 1554
Size: 743 90 573 148
Fragments arranged by size:
743 573 148 90
PvuII CAG'CTG
Cuts at: 0 744 981 1245 1554
Size: 744 237 264 309
Fragments arranged by size:
744 309 264 237
SacI G_AGCT'C
Cuts at: 0 1235 1554
Size: 1235 319
SacII CC_GC'GG
Cuts at: 0 333 1554
Size: 333 1221
SanDI GG'GwC_CC
Cuts at: 0 54 1554
Size: 54 1500
SapI GCTCTTCn'nnn_
Cuts at: 0 895 1554
Size: 895 659
SbfI CC_TGCA'GG
Cuts at: 0 1406 1554
Size: 1406 148
SexAI A'CCwGG_T
Cuts at: 0 381 1554
Size: 381 1173
SfcI C'TryA_G
Cuts at: 0 579 739 829 1402 1554
Size: 579 160 90 573 152
Fragments arranged by size:
579 573 160 152 90
SfoI GGC'GCC
Cuts at: 0 868 1515 1554
Size: 868 647 39
Fragments arranged by size:
868 647 39
SmaI CCC'GGG
Cuts at: 0 652 1519 1554
Size: 652 867 35
Fragments arranged by size:
867 652 35
SmlI C'TyrA_G
Cuts at: 0 192 260 314 1554
Size: 192 68 54 1240
Fragments arranged by size:
1240 192 68 54
StyI C'CwwG_G
Cuts at: 0 272 504 608 858 1554
Size: 272 232 104 250 696
Fragments arranged by size:
696 272 250 232 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 1554
Size: 270 1284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1003 1554
Size: 1003 551
XhoI C'TCGA_G
Cuts at: 0 314 1554
Size: 314 1240
XmaI C'CCGG_G
Cuts at: 0 650 1517 1554
Size: 650 867 37
Fragments arranged by size:
867 650 37
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BcgI BciVI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I KasI MmeI MslI MspA1I NarI
NcoI NspI PasI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI SapI SbfI SexAI
SfcI SfoI SmaI SmlI StyI TaqII XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BclI BfrBI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsiWI BsmI BsmBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SalI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XmnI ZraI