Restriction map for
pFN21AB7345
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccII AccIII AcyI AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AspHI AspI AviII AvrII
BalI BbeI BbrPI BglI BlnI
BsaAI BsaHI BsaI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I CpoI EaeI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FseI FspI HgaI Hin1I
KasI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
Tth111I
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 4363 GGTACC G^GTACC
AccI 1270 GTAGAC GT^[AC][GT]AC
AclI 2486 AACGTT AA^CGTT
AflIII 4911 ACATGT A^C[GA][CT]GT
Aor51HI 2852 AGCGCT AGC^GCT
Asp718I 4363 GGTACC G^GTACC
BcgI 2369 CGAAAGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4419 TGATCA T^GATCA
BsaWI 5141 TCCGGT [AT]^CCGG[AT]
BspDI 2834 ATCGAT AT^CGAT
BspLU11I 4911 ACATGT A^CATGT
ClaI 2834 ATCGAT AT^CGAT
DraIII 1447 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2852 AGCGCT AGC^GCT
EcoNI 529 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1315 GAATTC G^AATTC
EcoRV 2748 GATATC GAT^ATC
FbaI 4419 TGATCA T^GATCA
HaeII 2852 AGCGCT [GA]GCGC^[CT]
HindIII 3341 AAGCTT A^AGCTT
KpnI 4363 GGTACC GGTAC^C
MfeI 5123 CAATTG C^AATTG
MunI 5123 CAATTG C^AATTG
NheI 2689 GCTAGC G^CTAGC
PaeR7I 4298 CTCGAG C^TCGAG
PshAI 1248 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2486 AACGTT AA^CGTT
SapI 2824 GAAGAGC GCTCTTC(1/4)
SmaI 5256 CCCGGG CCC^GGG
SphI 1103 GCATGC GCATG^C
XhoI 4298 CTCGAG C^TCGAG
XmaCI 5256 CCCGGG C^CCGGG
XmaI 5256 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AvaI 4298 CTCGAG C^[CT]CG[GA]G
5256 CCCGGG
BbvCI 178 CCTCAGC CCTCAGC(-5/-2)
691 CCTCAGC
BciVI 3061 GGATAC GTATCC(6/5)
3114 GTATCC
BcnI 5256 CCCGG CC^[GC]GG
5257 CCGGG
BglII 828 AGATCT A^GATCT
1377 AGATCT
BsaBI 3436 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5860 GATCTTAATC
BsgI 71 CTGCAC GTGCAG(16/14)
5038 GTGCAG
BsmI 722 GCATTC GAATGC(1/-1)
2109 GCATTC
BsoBI 4298 CTCGAG C^[CT]CG[GA]G
5256 CCCGGG
DrdI 3763 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4306 GACAAAGTTGTC
HapII 5142 CCGG C^CGG
5257 CCGG
HpaI 318 GTTAAC GTT^AAC
793 GTTAAC
HpaII 5142 CCGG C^CGG
5257 CCGG
MaeII 1719 ACGT A^CGT
2487 ACGT
MamI 3436 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5860 GATCTTAATC
MspI 5142 CCGG C^CGG
5257 CCGG
NciI 5256 CCCGG CC^[GC]GG
5257 CCGGG
NcoI 1702 CCATGG C^CATGG
5098 CCATGG
NspI 1103 GCATGC [GA]CATG^[CT]
4911 ACATGT
PflMI 643 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAGCTCCTGG
ScaI 890 AGTACT AGT^ACT
2373 AGTACT
SexAI 4174 ACCTGGT A^CC[AT]GGT
4583 ACCAGGT
StuI 98 AGGCCT AGG^CCT
5220 AGGCCT
SwaI 3221 ATTTAAAT ATTT^AAAT
4832 ATTTAAAT
Van91I 643 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAGCTCCTGG
XbaI 831 TCTAGA T^CTAGA
5868 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AvaII BamHI BanI
BanII BbsI BbvI BfaI BfrI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI