(Circular) (Six-base) MAPSORT of: pFN21AB7345.seq Check: 8759 from: 1 to: 11374 pFN21AB7345 11374 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6454 6454 Size: 11374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9184 278 Size: 53 83 186 936 7648 2468 Fragments arranged by size: 7648 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3276 8717 1937 Size: 1339 5441 4594 Fragments arranged by size: 5441 4594 1339 Acc65I G'GTAC_C Cuts at: 6368 8695 6368 Size: 2327 9047 AclI AA'CG_TT Cuts at: 1563 4492 9501 9874 1563 Size: 2929 5009 373 3063 Fragments arranged by size: 5009 3063 2929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 2742 2807 3156 3240 3291 3396 3546 Size: 550 560 65 349 84 51 105 150 Cuts at: 3546 3845 4062 4370 5463 6219 6717 7542 8025 Size: 299 217 308 1093 756 498 825 483 Cuts at: 8025 9437 10821 11089 1632 Size: 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1384 1093 825 756 560 550 498 483 349 308 299 268 217 150 105 84 65 51 AfeI AGC'GCT Cuts at: 4859 4859 Size: 11374 AflII C'TTAA_G Cuts at: 820 1017 4861 7361 8267 820 Size: 197 3844 2500 906 3927 Fragments arranged by size: 3927 3844 2500 906 197 AflIII A'CryG_T Cuts at: 1203 6916 8671 10273 11250 1203 Size: 5713 1755 1602 977 1327 Fragments arranged by size: 5713 1755 1602 1327 977 AgeI A'CCGG_T Cuts at: 10205 10205 Size: 11374 AhdI GACnn_n'nnGTC Cuts at: 10103 10103 Size: 11374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 11374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3566 8079 1184 Size: 2382 4513 4479 Fragments arranged by size: 4513 4479 2382 AlwNI CAG_nnn'CTG Cuts at: 3203 3890 4148 5636 6278 6531 9117 10689 3203 Size: 687 258 1488 642 253 2586 1572 3888 Fragments arranged by size: 3888 2586 1572 1488 687 642 258 253 ApaLI G'TGCA_C Cuts at: 9431 10587 9431 Size: 1156 10218 ApoI r'AATT_y Cuts at: 1493 2393 2414 3320 3625 3680 4033 4701 7987 Size: 900 21 906 305 55 353 668 3286 Cuts at: 7987 8034 8683 8909 9061 11170 1493 Size: 47 649 226 152 2109 1697 Fragments arranged by size: 3286 2109 1697 906 900 668 649 353 305 226 152 55 47 21 AseI AT'TA_AT Cuts at: 160 1032 2235 3120 8202 9928 160 Size: 872 1203 885 5082 1726 1606 Fragments arranged by size: 5082 1726 1606 1203 885 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11374 AvaI C'yCGr_G Cuts at: 1870 1943 6303 7261 8699 1870 Size: 73 4360 958 1438 4545 Fragments arranged by size: 4545 4360 1438 958 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5310 5343 7116 7149 5310 Size: 33 1773 33 9535 Fragments arranged by size: 9535 1773 33 33 BamHI G'GATC_C Cuts at: 5446 5903 6757 8704 9071 5446 Size: 457 854 1947 367 7749 Fragments arranged by size: 7749 1947 854 457 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2657 5431 6176 6215 Size: 325 217 627 221 649 2774 745 39 Cuts at: 6215 6368 7058 7265 8695 10150 618 Size: 153 690 207 1430 1455 1842 Fragments arranged by size: 2774 1842 1455 1430 745 690 649 627 325 221 217 207 153 39 BanII G_rGCy'C Cuts at: 1391 5703 7283 8028 8693 1391 Size: 4312 1580 745 665 4072 Fragments arranged by size: 4312 4072 1580 745 665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 2619 6315 7919 8638 928 Size: 971 567 153 3696 1604 719 3664 Fragments arranged by size: 3696 3664 1604 971 719 567 153 BbvCI CC'TCA_GC Cuts at: 2184 2697 2184 Size: 513 10861 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 9565 9599 1903 Size: 34 2426 34 5168 34 3678 Fragments arranged by size: 5168 3678 2426 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5060 5130 9266 10476 5060 Size: 70 4136 1210 5958 Fragments arranged by size: 5958 4136 1210 70 BclI T'GATC_A Cuts at: 1548 6424 1548 Size: 4876 6498 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9985 136 Size: 107 122 71 631 605 8313 1525 Fragments arranged by size: 8313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2833 3382 11369 2833 Size: 549 7987 2838 Fragments arranged by size: 7987 2838 549 BlpI GC'TnA_GC Cuts at: 2718 4191 4650 8791 2718 Size: 1473 459 4141 5301 Fragments arranged by size: 5301 4141 1473 459 Bme1580I G_kGCm'C Cuts at: 1790 2662 3029 3336 3555 7261 9435 10591 1790 Size: 872 367 307 219 3706 2174 1156 2573 Fragments arranged by size: 3706 2573 2174 1156 872 367 307 219 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2587 3390 5688 Size: 416 53 83 399 24 1170 803 2298 Cuts at: 5688 7278 8038 9105 10063 442 Size: 1590 760 1067 958 1753 Fragments arranged by size: 2298 1753 1590 1170 1067 958 803 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4698 1056 Size: 3642 7732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2390 4232 4369 6117 7176 10034 1239 Size: 971 180 1842 137 1748 1059 2858 2579 Fragments arranged by size: 2858 2579 1842 1748 1059 971 180 137 Bpu10I CC'TnA_GC Cuts at: 2184 2697 2184 Size: 513 10861 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 3793 3957 4799 7259 7451 7897 8288 9499 Size: 815 164 842 2460 192 446 391 1211 Cuts at: 9499 10364 10662 2978 Size: 865 298 3690 Fragments arranged by size: 3690 2460 1211 865 842 815 446 391 298 192 164 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 10037 882 Size: 617 8538 2219 Fragments arranged by size: 8538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 9165 493 Size: 711 7961 2702 Fragments arranged by size: 7961 2702 711 BsaBI GATnn'nnATC Cuts at: 5445 7869 11368 5445 Size: 2424 3499 5451 Fragments arranged by size: 5451 3499 2424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 9181 9563 10198 275 Size: 53 83 186 936 7648 382 635 1451 Fragments arranged by size: 7648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7146 9807 10205 10479 10626 11037 7146 Size: 2661 398 274 147 411 7483 Fragments arranged by size: 7483 2661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 3644 3674 6139 Size: 30 165 30 616 30 1595 30 2465 Cuts at: 6139 6169 7984 8014 8154 8184 1178 Size: 30 1815 30 140 30 4368 Fragments arranged by size: 4368 2465 1815 1595 616 165 140 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2622 4868 6120 7451 7454 1186 Size: 1436 2246 1252 1331 3 5106 Fragments arranged by size: 5106 2246 1436 1331 1252 3 BseYI C'CCAG_C Cuts at: 8354 10577 11073 8354 Size: 2223 496 8655 Fragments arranged by size: 8655 2223 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 7064 1163 Size: 573 325 5003 5473 Fragments arranged by size: 5473 5003 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8824 9585 10613 1540 Size: 275 186 6823 761 1028 2301 Fragments arranged by size: 6823 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 9435 9520 10591 9435 Size: 85 1071 10218 Fragments arranged by size: 10218 1071 85 BsmI GAATG_Cn' Cuts at: 2727 4114 8885 8978 2727 Size: 1387 4771 93 5123 Fragments arranged by size: 5123 4771 1387 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2662 3029 3336 3555 5703 7261 7283 Size: 399 872 367 307 219 2148 1558 22 Cuts at: 7283 8028 8693 9435 9520 10591 1391 Size: 745 665 742 85 1071 2174 Fragments arranged by size: 2174 2148 1558 1071 872 745 742 665 399 367 307 219 85 22 BspEI T'CCGG_A Cuts at: 11037 11037 Size: 11374 BspHI T'CATG_A Cuts at: 3508 3580 3739 3957 4182 4267 6720 9263 10993 Size: 72 159 218 225 85 2453 2543 1730 Cuts at: 10993 3508 Size: 3889 Fragments arranged by size: 3889 2543 2453 1730 225 218 159 85 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3196 6454 6702 7342 8319 8729 844 Size: 2352 3258 248 640 977 410 3489 Fragments arranged by size: 3489 3258 2352 977 640 410 248 BsrBI CCG'CTC Cuts at: 1394 1823 9261 1394 Size: 429 7438 3507 Fragments arranged by size: 7438 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2109 4515 7456 9869 10043 65 Size: 1183 861 2406 2941 2413 174 1396 Fragments arranged by size: 2941 2413 2406 1396 1183 861 174 BsrFI r'CCGG_y Cuts at: 1700 1810 10018 10205 1700 Size: 110 8208 187 2869 Fragments arranged by size: 8208 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11374 BssSI C'ACGA_G Cuts at: 9434 10446 9434 Size: 1012 10362 BstAPI GCAn_nnn'nTGC Cuts at: 794 3352 3655 5930 8195 794 Size: 2558 303 2275 2265 3973 Fragments arranged by size: 3973 2558 2275 2265 303 BstBI TT'CG_AA Cuts at: 10178 10178 Size: 11374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11374 BstYI r'GATC_y Cuts at: 1972 2258 2833 3382 4081 5001 5363 5446 5903 Size: 286 575 549 699 920 362 83 457 Cuts at: 5903 6274 6757 8704 9071 9456 9473 10925 11011 Size: 371 483 1947 367 385 17 1452 86 Cuts at: 11011 11023 11369 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1947 1452 920 699 575 549 483 457 385 371 367 362 346 286 86 83 17 12 Bsu36I CC'TnA_GG Cuts at: 2562 5238 6762 2562 Size: 2676 1524 7174 Fragments arranged by size: 7174 2676 1524 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 3707 3908 7103 513 Size: 552 939 162 1541 201 3195 4784 Fragments arranged by size: 4784 3195 1541 939 552 201 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10635 Fragments arranged by size: 10635 683 56 BtsI GCAGTG_nn' Cuts at: 3555 7965 8890 9684 9704 3555 Size: 4410 925 794 20 5225 Fragments arranged by size: 5225 4410 925 794 20 ClaI AT'CG_AT Cuts at: 1554 4840 1554 Size: 3286 8088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8425 8460 529 Size: 35 7861 35 3443 Fragments arranged by size: 7861 3443 35 35 DraI TTT'AAA Cuts at: 4369 5229 6840 7741 8335 8678 4369 Size: 860 1611 901 594 343 7065 Fragments arranged by size: 7065 1611 901 860 594 343 DraIII CAC_nnn'GTG Cuts at: 3457 3457 Size: 11374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5774 6317 10381 809 Size: 507 4458 543 4064 1802 Fragments arranged by size: 4458 4064 1802 543 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 8821 9710 11339 Size: 54 1425 50 275 18 6991 889 1629 Cuts at: 11339 8 Size: 43 Fragments arranged by size: 6991 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8821 1537 Size: 275 7009 4090 Fragments arranged by size: 7009 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 4824 4845 5013 5253 5277 5598 9304 Size: 848 2623 21 168 240 24 321 3706 Cuts at: 9304 1353 Size: 3423 Fragments arranged by size: 3706 3423 2623 848 321 240 168 24 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9961 10335 10481 387 Size: 1064 138 8372 374 146 1280 Fragments arranged by size: 8372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2210 2390 2742 2807 3156 3240 Size: 393 550 28 180 352 65 349 84 Cuts at: 3240 3291 3396 3546 3845 4062 4232 4369 4370 Size: 51 105 150 299 217 170 137 1 Cuts at: 4370 5463 6117 6219 6717 7176 7542 8025 9437 Size: 1093 654 102 498 459 366 483 1412 Cuts at: 9437 10034 10821 11089 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1412 1093 787 654 597 550 498 483 459 393 366 352 349 299 268 217 180 170 150 137 105 102 84 65 51 28 1 EcoNI CCTnn'n_nnAGG Cuts at: 2538 2538 Size: 11374 EcoO109I rG'GnC_Cy Cuts at: 2179 3560 4390 6443 7196 2179 Size: 1381 830 2053 753 6357 Fragments arranged by size: 6357 2053 1381 830 753 EcoRI G'AATT_C Cuts at: 3320 8683 9061 3320 Size: 5363 378 5633 Fragments arranged by size: 5633 5363 378 EcoRV GAT'ATC Cuts at: 4755 4755 Size: 11374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2162 2837 4400 5591 2162 Size: 675 1563 1191 7945 Fragments arranged by size: 7945 1563 1191 675 FspI TGC'GCA Cuts at: 9880 9880 Size: 11374 HaeII r_GCGC'y Cuts at: 1678 4861 10521 1678 Size: 3183 5660 2531 Fragments arranged by size: 5660 3183 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3644 3676 4744 4776 4931 Size: 32 135 32 2072 32 1068 32 155 Cuts at: 4931 4963 5506 5538 5929 5961 6340 6372 6854 Size: 32 543 32 391 32 379 32 482 Cuts at: 6854 6886 10015 10047 10089 10121 1373 Size: 32 3129 32 42 32 2626 Fragments arranged by size: 3129 2626 2072 1068 543 482 391 379 155 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2325 2800 5709 8718 8964 669 Size: 988 281 387 475 2909 3009 246 3079 Fragments arranged by size: 3079 3009 2909 988 475 387 281 246 HindIII A'AGCT_T Cuts at: 748 5346 748 Size: 4598 6776 HpaI GTT'AAC Cuts at: 2325 2800 8964 2325 Size: 475 6164 4735 Fragments arranged by size: 6164 4735 475 KpnI G_GTAC'C Cuts at: 6372 8699 6372 Size: 2327 9047 MfeI C'AATT_G Cuts at: 7128 8973 7128 Size: 1845 9529 MluI A'CGCG_T Cuts at: 11250 11250 Size: 11374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2236 4516 5516 5877 8063 8140 8293 8425 Size: 931 2280 1000 361 2186 77 153 132 Cuts at: 8425 10488 10672 1305 Size: 2063 184 2007 Fragments arranged by size: 2280 2186 2063 2007 1000 931 361 184 153 132 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 9552 Fragments arranged by size: 9552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3329 3962 4530 5229 6111 6363 7102 Size: 648 2163 633 568 699 882 252 739 Cuts at: 7102 8283 9332 9691 9850 518 Size: 1181 1049 359 159 2042 Fragments arranged by size: 2163 2042 1181 1049 882 739 699 648 633 568 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2978 4162 6641 6700 7847 8399 8441 8790 Size: 1372 1184 2479 59 1147 552 42 349 Cuts at: 8790 9467 10615 10860 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2479 2120 1372 1184 1148 1147 677 552 349 245 59 42 NcoI C'CATG_G Cuts at: 513 1065 2004 3707 7103 513 Size: 552 939 1703 3396 4784 Fragments arranged by size: 4784 3396 1703 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11374 NheI G'CTAG_C Cuts at: 1052 4694 1052 Size: 3642 7732 NotI GC'GGCC_GC Cuts at: 8821 8821 Size: 11374 NspI r_CATG'y Cuts at: 1132 1229 3112 6920 8675 8732 10277 1132 Size: 97 1883 3808 1755 57 1545 2229 Fragments arranged by size: 3808 2229 1883 1755 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 8584 1348 Size: 7236 4138 PciI A'CATG_T Cuts at: 6916 8671 10273 6916 Size: 1755 1602 8017 Fragments arranged by size: 8017 1755 1602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2654 3203 1108 Size: 1546 549 9279 Fragments arranged by size: 9279 1546 549 PfoI T'CCnGG_A Cuts at: 3069 3202 7192 3069 Size: 133 3990 7251 Fragments arranged by size: 7251 3990 133 PmeI GTTT'AAAC Cuts at: 8678 8678 Size: 11374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2052 2084 8165 8197 8501 8533 9443 9475 10956 Size: 32 6081 32 304 32 910 32 1481 Cuts at: 10956 10988 2052 Size: 32 2438 Fragments arranged by size: 6081 2438 1481 910 304 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 4390 6443 7196 2179 Size: 2211 2053 753 6357 Fragments arranged by size: 6357 2211 2053 753 PshAI GACnn'nnGTC Cuts at: 1613 3257 1613 Size: 1644 9730 PsiI TTA'TAA Cuts at: 8944 8944 Size: 11374 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 11342 PstI C_TGCA'G Cuts at: 830 2781 7353 7407 7951 8058 8330 8726 830 Size: 1951 4572 54 544 107 272 396 3478 Fragments arranged by size: 4572 3478 1951 544 396 272 107 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11374 PvuII CAG'CTG Cuts at: 2978 4162 6641 6700 7847 8399 8441 2978 Size: 1184 2479 59 1147 552 42 5911 Fragments arranged by size: 5911 2479 1184 1147 552 59 42 SalI G'TCGA_C Cuts at: 1936 8716 1936 Size: 6780 4594 SapI GCTCTTCn'nnn_ Cuts at: 4824 4824 Size: 11374 SbfI CC_TGCA'GG Cuts at: 8726 8726 Size: 11374 ScaI AGT'ACT Cuts at: 2897 4380 9622 11334 2897 Size: 1483 5242 1712 2937 Fragments arranged by size: 5242 2937 1712 1483 SexAI A'CCwGG_T Cuts at: 6179 6588 6179 Size: 409 10965 SfcI C'TryA_G Cuts at: 826 1045 2289 2777 3611 5730 7292 7349 7403 Size: 219 1244 488 834 2119 1562 57 54 Cuts at: 7403 7947 8054 8326 8722 9857 10538 10729 826 Size: 544 107 272 396 1135 681 191 1471 Fragments arranged by size: 2119 1562 1471 1244 1135 834 681 544 488 396 272 219 191 107 57 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11374 SmaI CCC'GGG Cuts at: 1872 7263 8701 1872 Size: 5391 1438 4545 Fragments arranged by size: 5391 4545 1438 SmlI C'TyrA_G Cuts at: 820 1017 1943 2957 3772 3972 4814 4861 6303 Size: 197 926 1014 815 200 842 47 1442 Cuts at: 6303 7274 7361 7466 7912 8267 8303 9478 10379 Size: 971 87 105 446 355 36 1175 901 Cuts at: 10379 10641 820 Size: 262 1553 Fragments arranged by size: 1553 1442 1175 1014 971 926 901 842 815 446 355 262 200 197 105 87 47 36 SnaBI TAC'GTA Cuts at: 493 9165 493 Size: 8672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11374 SphI G_CATG'C Cuts at: 1132 3112 8732 1132 Size: 1980 5620 3774 Fragments arranged by size: 5620 3774 1980 SspI AAT'ATT Cuts at: 5 52 3046 4508 5466 9298 5 Size: 47 2994 1462 958 3832 2081 Fragments arranged by size: 3832 2994 2081 1462 958 47 StuI AGG'CCT Cuts at: 1517 2105 7227 1517 Size: 588 5122 5664 Fragments arranged by size: 5664 5122 588 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 3707 7103 8460 8813 513 Size: 552 939 501 1202 3396 1357 353 3074 Fragments arranged by size: 3396 3074 1357 1202 939 552 501 353 SwaI ATTT'AAAT Cuts at: 5229 6840 5229 Size: 1611 9763 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2606 3409 4457 5607 8368 9401 Size: 1255 446 580 803 1048 1150 2761 1033 Cuts at: 9401 9418 9571 9756 10201 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2761 1498 1255 1150 1048 1033 803 580 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 2961 4378 Size: 274 80 33 51 1443 918 66 1417 Cuts at: 4378 6645 9620 11332 96 Size: 2267 2975 1712 138 Fragments arranged by size: 2975 2267 1712 1443 1417 918 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 3443 3475 3862 3894 7619 7651 8154 Size: 32 1392 32 387 32 3725 32 503 Cuts at: 8154 8186 8355 8387 11334 11366 2019 Size: 32 169 32 2947 32 2027 Fragments arranged by size: 3725 2947 2027 1392 503 387 169 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11374 XbaI T'CTAG_A Cuts at: 2836 7873 8710 2836 Size: 5037 837 5500 Fragments arranged by size: 5500 5037 837 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2116 4288 4532 4651 2116 Size: 2172 244 119 8839 Fragments arranged by size: 8839 2172 244 119 XhoI C'TCGA_G Cuts at: 1943 6303 1943 Size: 4360 7014 XmaI C'CCGG_G Cuts at: 1870 7261 8699 1870 Size: 5391 1438 4545 Fragments arranged by size: 5391 4545 1438 XmnI GAAnn'nnTTC Cuts at: 2817 3684 4788 6966 7167 7961 9503 2817 Size: 867 1104 2178 201 794 1542 4688 Fragments arranged by size: 4688 2178 1542 1104 867 794 201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9182 276 Size: 53 83 186 936 7648 2468 Fragments arranged by size: 7648 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI