Restriction map for
pFN21AB7345
(Linear) (Six-base) MAPSORT of: KIBB7345.seq Check: 766 from: 1 to: 6669
KIBB7345.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4449 6669
Size: 4449 2220
AccI GT'mk_AC
Cuts at: 0 1271 6669
Size: 1271 5398
Acc65I G'GTAC_C
Cuts at: 0 4363 6669
Size: 4363 2306
AclI AA'CG_TT
Cuts at: 0 2487 6669
Size: 2487 4182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 737 802 1151 1235 1286 1391 1541
Size: 177 560 65 349 84 51 105 150
Cuts at: 1541 1840 2057 2365 3458 4214 4712 5537 6020
Size: 299 217 308 1093 756 498 825 483
Cuts at: 6020 6669
Size: 649
Fragments arranged by size:
1093 825 756 649 560 498 483 349
308 299 217 177 150 105 84 65
51
AfeI AGC'GCT
Cuts at: 0 2854 6669
Size: 2854 3815
AflII C'TTAA_G
Cuts at: 0 2856 5356 6262 6669
Size: 2856 2500 906 407
Fragments arranged by size:
2856 2500 906 407
AflIII A'CryG_T
Cuts at: 0 4911 6669
Size: 4911 1758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1561 6074 6669
Size: 1561 4513 595
Fragments arranged by size:
4513 1561 595
AlwNI CAG_nnn'CTG
Cuts at: 0 1198 1885 2143 3631 4273 4526 6669
Size: 1198 687 258 1488 642 253 2143
Fragments arranged by size:
2143 1488 1198 687 642 258 253
ApoI r'AATT_y
Cuts at: 0 388 409 1315 1620 1675 2028 2696 5982
Size: 388 21 906 305 55 353 668 3286
Cuts at: 5982 6029 6669
Size: 47 640
Fragments arranged by size:
3286 906 668 640 388 353 305 55
47 21
AseI AT'TA_AT
Cuts at: 0 230 1115 6197 6669
Size: 230 885 5082 472
Fragments arranged by size:
5082 885 472 230
AvaI C'yCGr_G
Cuts at: 0 4298 5256 6669
Size: 4298 958 1413
Fragments arranged by size:
4298 1413 958
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3305 3338 5111 5144 6669
Size: 3305 33 1773 33 1525
Fragments arranged by size:
3305 1773 1525 33 33
BamHI G'GATC_C
Cuts at: 0 3441 3898 4752 6669
Size: 3441 457 854 1917
Fragments arranged by size:
3441 1917 854 457
BanI G'GyrC_C
Cuts at: 0 3 652 3426 4171 4210 4363 5053 5260
Size: 3 649 2774 745 39 153 690 207
Cuts at: 5260 6669
Size: 1409
Fragments arranged by size:
2774 1409 745 690 649 207 153 39
3
BanII G_rGCy'C
Cuts at: 0 3698 5278 6023 6669
Size: 3698 1580 745 646
Fragments arranged by size:
3698 1580 745 646
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 614 4310 5914 6633 6669
Size: 461 153 3696 1604 719 36
Fragments arranged by size:
3696 1604 719 461 153 36
BbvCI CC'TCA_GC
Cuts at: 0 179 692 6669
Size: 179 513 5977
Fragments arranged by size:
5977 513 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2358 2392 6669
Size: 2358 34 4277
Fragments arranged by size:
4277 2358 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3055 3125 6669
Size: 3055 70 3544
Fragments arranged by size:
3544 3055 70
BclI T'GATC_A
Cuts at: 0 4419 6669
Size: 4419 2250
BglII A'GATC_T
Cuts at: 0 828 1377 6669
Size: 828 549 5292
Fragments arranged by size:
5292 828 549
BlpI GC'TnA_GC
Cuts at: 0 713 2186 2645 6669
Size: 713 1473 459 4024
Fragments arranged by size:
4024 1473 713 459
Bme1580I G_kGCm'C
Cuts at: 0 657 1024 1331 1550 5256 6669
Size: 657 367 307 219 3706 1413
Fragments arranged by size:
3706 1413 657 367 307 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 582 1385 3683 5273 6033 6669
Size: 582 803 2298 1590 760 636
Fragments arranged by size:
2298 1590 803 760 636 582
BmtI G_CTAG'C
Cuts at: 0 2693 6669
Size: 2693 3976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 385 2227 2364 4112 5171 6669
Size: 205 180 1842 137 1748 1059 1498
Fragments arranged by size:
1842 1748 1498 1059 205 180 137
Bpu10I CC'TnA_GC
Cuts at: 0 179 692 6669
Size: 179 513 5977
Fragments arranged by size:
5977 513 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1788 1952 2794 5254 5446 5892 6283
Size: 973 815 164 842 2460 192 446 391
Cuts at: 6283 6669
Size: 386
Fragments arranged by size:
2460 973 842 815 446 391 386 192
164
BsaBI GATnn'nnATC
Cuts at: 0 3440 5864 6669
Size: 3440 2424 805
Fragments arranged by size:
3440 2424 805
BsaWI w'CCGG_w
Cuts at: 0 5141 6669
Size: 5141 1528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 1639 1669 4134 4164 5979 6009
Size: 14 30 1595 30 2465 30 1815 30
Cuts at: 6009 6149 6179 6669
Size: 140 30 490
Fragments arranged by size:
2465 1815 1595 490 140 30 30 30
30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 2863 4115 5446 5449 6669
Size: 617 2246 1252 1331 3 1220
Fragments arranged by size:
2246 1331 1252 1220 617 3
BseYI C'CCAG_C
Cuts at: 0 6349 6669
Size: 6349 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 5059 6669
Size: 56 5003 1610
Fragments arranged by size:
5003 1610 56
BsmI GAATG_Cn'
Cuts at: 0 722 2109 6669
Size: 722 1387 4560
Fragments arranged by size:
4560 1387 722
Bsp1286I G_dGCh'C
Cuts at: 0 657 1024 1331 1550 3698 5256 5278 6023
Size: 657 367 307 219 2148 1558 22 745
Cuts at: 6023 6669
Size: 646
Fragments arranged by size:
2148 1558 745 657 646 367 307 219
22
BspHI T'CATG_A
Cuts at: 0 1503 1575 1734 1952 2177 2262 4715 6669
Size: 1503 72 159 218 225 85 2453 1954
Fragments arranged by size:
2453 1954 1503 225 218 159 85 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1191 4449 4697 5337 6314 6669
Size: 1191 3258 248 640 977 355
Fragments arranged by size:
3258 1191 977 640 355 248
BsrDI GCAATG_nn'
Cuts at: 0 104 2510 5451 6669
Size: 104 2406 2941 1218
Fragments arranged by size:
2941 2406 1218 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1347 1650 3925 6190 6669
Size: 1347 303 2275 2265 479
Fragments arranged by size:
2275 2265 1347 479 303
BstYI r'GATC_y
Cuts at: 0 253 828 1377 2076 2996 3358 3441 3898
Size: 253 575 549 699 920 362 83 457
Cuts at: 3898 4269 4752 6669
Size: 371 483 1917
Fragments arranged by size:
1917 920 699 575 549 483 457 371
362 253 83
Bsu36I CC'TnA_GG
Cuts at: 0 557 3233 4757 6669
Size: 557 2676 1524 1912
Fragments arranged by size:
2676 1912 1524 557
BtgI C'CryG_G
Cuts at: 0 161 1702 1903 5098 6669
Size: 161 1541 201 3195 1571
Fragments arranged by size:
3195 1571 1541 201 161
BtsI GCAGTG_nn'
Cuts at: 0 1550 5960 6669
Size: 1550 4410 709
Fragments arranged by size:
4410 1550 709
ClaI AT'CG_AT
Cuts at: 0 2835 6669
Size: 2835 3834
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6420 6455 6669
Size: 6420 35 214
Fragments arranged by size:
6420 214 35
DraI TTT'AAA
Cuts at: 0 2364 3224 4835 5736 6330 6669
Size: 2364 860 1611 901 594 339
Fragments arranged by size:
2364 1611 901 860 594 339
DraIII CAC_nnn'GTG
Cuts at: 0 1452 6669
Size: 1452 5217
DrdI GACnn_nn'nnGTC
Cuts at: 0 3769 4312 6669
Size: 3769 543 2357
Fragments arranged by size:
3769 2357 543
EarI CTCTTCn'nnn_
Cuts at: 0 196 2819 2840 3008 3248 3272 3593 6669
Size: 196 2623 21 168 240 24 321 3076
Fragments arranged by size:
3076 2623 321 240 196 168 24 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 205 385 737 802 1151 1235 1286
Size: 177 28 180 352 65 349 84 51
Cuts at: 1286 1391 1541 1840 2057 2227 2364 2365 3458
Size: 105 150 299 217 170 137 1 1093
Cuts at: 3458 4112 4214 4712 5171 5537 6020 6669
Size: 654 102 498 459 366 483 649
Fragments arranged by size:
1093 654 649 498 483 459 366 352
349 299 217 180 177 170 150 137
105 102 84 65 51 28 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 533 6669
Size: 533 6136
EcoO109I rG'GnC_Cy
Cuts at: 0 174 1555 2385 4438 5191 6669
Size: 174 1381 830 2053 753 1478
Fragments arranged by size:
2053 1478 1381 830 753 174
EcoRI G'AATT_C
Cuts at: 0 1315 6669
Size: 1315 5354
EcoRV GAT'ATC
Cuts at: 0 2750 6669
Size: 2750 3919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 157 832 2395 3586 6669
Size: 157 675 1563 1191 3083
Fragments arranged by size:
3083 1563 1191 675 157
HaeII r_GCGC'y
Cuts at: 0 2856 6669
Size: 2856 3813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1639 1671 2739 2771 2926 2958 3501 3533
Size: 1639 32 1068 32 155 32 543 32
Cuts at: 3533 3924 3956 4335 4367 4849 4881 6669
Size: 391 32 379 32 482 32 1788
Fragments arranged by size:
1788 1639 1068 543 482 391 379 155
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 320 795 3704 6669
Size: 320 475 2909 2965
Fragments arranged by size:
2965 2909 475 320
HindIII A'AGCT_T
Cuts at: 0 3341 6669
Size: 3341 3328
HpaI GTT'AAC
Cuts at: 0 320 795 6669
Size: 320 475 5874
Fragments arranged by size:
5874 475 320
KpnI G_GTAC'C
Cuts at: 0 4367 6669
Size: 4367 2302
MfeI C'AATT_G
Cuts at: 0 5123 6669
Size: 5123 1546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 2511 3511 3872 6058 6135 6288 6420
Size: 231 2280 1000 361 2186 77 153 132
Cuts at: 6420 6669
Size: 249
Fragments arranged by size:
2280 2186 1000 361 249 231 153 132
77
MslI CAynn'nnrTG
Cuts at: 0 1324 1957 2525 3224 4106 4358 5097 6278
Size: 1324 633 568 699 882 252 739 1181
Cuts at: 6278 6669
Size: 391
Fragments arranged by size:
1324 1181 882 739 699 633 568 391
252
MspA1I CmG'CkG
Cuts at: 0 973 2157 4636 4695 5842 6394 6436 6669
Size: 973 1184 2479 59 1147 552 42 233
Fragments arranged by size:
2479 1184 1147 973 552 233 59 42
NcoI C'CATG_G
Cuts at: 0 1702 5098 6669
Size: 1702 3396 1571
Fragments arranged by size:
3396 1702 1571
NheI G'CTAG_C
Cuts at: 0 2689 6669
Size: 2689 3980
NspI r_CATG'y
Cuts at: 0 1107 4915 6669
Size: 1107 3808 1754
Fragments arranged by size:
3808 1754 1107
PasI CC'CwG_GG
Cuts at: 0 6579 6669
Size: 6579 90
PciI A'CATG_T
Cuts at: 0 4911 6669
Size: 4911 1758
PflMI CCAn_nnn'nTGG
Cuts at: 0 649 1198 6669
Size: 649 549 5471
Fragments arranged by size:
5471 649 549
PfoI T'CCnGG_A
Cuts at: 0 1064 1197 5187 6669
Size: 1064 133 3990 1482
Fragments arranged by size:
3990 1482 1064 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 6160 6192 6496 6528 6669
Size: 47 32 6081 32 304 32 141
Fragments arranged by size:
6081 304 141 47 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 174 2385 4438 5191 6669
Size: 174 2211 2053 753 1478
Fragments arranged by size:
2211 2053 1478 753 174
PshAI GACnn'nnGTC
Cuts at: 0 1252 6669
Size: 1252 5417
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3620 3652 6669
Size: 3620 32 3017
Fragments arranged by size:
3620 3017 32
PstI C_TGCA'G
Cuts at: 0 776 5348 5402 5946 6053 6325 6669
Size: 776 4572 54 544 107 272 344
Fragments arranged by size:
4572 776 544 344 272 107 54
PvuII CAG'CTG
Cuts at: 0 973 2157 4636 4695 5842 6394 6436 6669
Size: 973 1184 2479 59 1147 552 42 233
Fragments arranged by size:
2479 1184 1147 973 552 233 59 42
SapI GCTCTTCn'nnn_
Cuts at: 0 2819 6669
Size: 2819 3850
ScaI AGT'ACT
Cuts at: 0 892 2375 6669
Size: 892 1483 4294
Fragments arranged by size:
4294 1483 892
SexAI A'CCwGG_T
Cuts at: 0 4174 4583 6669
Size: 4174 409 2086
Fragments arranged by size:
4174 2086 409
SfcI C'TryA_G
Cuts at: 0 284 772 1606 3725 5287 5344 5398 5942
Size: 284 488 834 2119 1562 57 54 544
Cuts at: 5942 6049 6321 6669
Size: 107 272 348
Fragments arranged by size:
2119 1562 834 544 488 348 284 272
107 57 54
SmaI CCC'GGG
Cuts at: 0 5258 6669
Size: 5258 1411
SmlI C'TyrA_G
Cuts at: 0 952 1767 1967 2809 2856 4298 5269 5356
Size: 952 815 200 842 47 1442 971 87
Cuts at: 5356 5461 5907 6262 6298 6669
Size: 105 446 355 36 371
Fragments arranged by size:
1442 971 952 842 815 446 371 355
200 105 87 47 36
SphI G_CATG'C
Cuts at: 0 1107 6669
Size: 1107 5562
SspI AAT'ATT
Cuts at: 0 1041 2503 3461 6669
Size: 1041 1462 958 3208
Fragments arranged by size:
3208 1462 1041 958
StuI AGG'CCT
Cuts at: 0 100 5222 6669
Size: 100 5122 1447
Fragments arranged by size:
5122 1447 100
StyI C'CwwG_G
Cuts at: 0 500 1702 5098 6455 6669
Size: 500 1202 3396 1357 214
Fragments arranged by size:
3396 1357 1202 500 214
SwaI ATTT'AAAT
Cuts at: 0 3224 4835 6669
Size: 3224 1611 1834
Fragments arranged by size:
3224 1834 1611
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 601 1404 2452 3602 6363 6669
Size: 21 580 803 1048 1150 2761 306
Fragments arranged by size:
2761 1150 1048 803 580 306 21
TatI w'GTAC_w
Cuts at: 0 890 956 2373 4640 6669
Size: 890 66 1417 2267 2029
Fragments arranged by size:
2267 2029 1417 890 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 1438 1470 1857 1889 5614 5646
Size: 14 32 1392 32 387 32 3725 32
Cuts at: 5646 6149 6181 6350 6382 6669
Size: 503 32 169 32 287
Fragments arranged by size:
3725 1392 503 387 287 169 32 32
32 32 32 32 14
XbaI T'CTAG_A
Cuts at: 0 831 5868 6669
Size: 831 5037 801
Fragments arranged by size:
5037 831 801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 2283 2527 2646 6669
Size: 111 2172 244 119 4023
Fragments arranged by size:
4023 2172 244 119 111
XhoI C'TCGA_G
Cuts at: 0 4298 6669
Size: 4298 2371
XmaI C'CCGG_G
Cuts at: 0 5256 6669
Size: 5256 1413
XmnI GAAnn'nnTTC
Cuts at: 0 812 1679 2783 4961 5162 5956 6669
Size: 812 867 1104 2178 201 794 713
Fragments arranged by size:
2178 1104 867 812 794 713 201
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AlfI AlwNI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI
BsrDI BstAPI BstYI Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MmeI MslI MspA1I NcoI NheI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PsrI PstI
PvuII SapI ScaI SexAI SfcI SmaI SmlI SphI
SspI StuI StyI SwaI TaqII TatI TstI XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AleI AloI ApaI ApaLI AscI
AsiSI AvrII BbeI BfrBI BglI BmgBI BplI BsaI
BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI EaeI EagI EciI EcoICRI FseI FspI FspAI
KasI MluI MscI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PmeI PmlI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI Tth111I ZraI