Restriction map for
pFN21AB7322
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BglI
BlnI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HgaI Hin1I KasI KpnI
MluI MroI NaeI NarI NgoMIV
NotI NruI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PvuI
RsrII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 5916 CTTAAG C^TTAAG
AspI 612 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4629 GGATCC G^GATCC
BbvCI 4682 CCTCAGC CCTCAGC(-5/-2)
BcgI 688 GCAGGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4622 TGATCA T^GATCA
BfrI 5916 CTTAAG C^TTAAG
BglII 1863 AGATCT A^GATCT
BsaBI 5438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 146 CGTCTC CGTCTC(1/5)
Bsp1407I 4315 TGTACA T^GTACA
BspHI 2493 TCATGA T^CATGA
BsrGI 4315 TGTACA T^GTACA
BstAPI 862 GCAATGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI 1476 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4455 GATATC GAT^ATC
EcoT22I 865 ATGCAT ATGCA^T
FbaI 4622 TGATCA T^GATCA
HaeII 2502 GGCGCT [GA]GCGC^[CT]
KspI 89 CCGCGG CCGC^GG
MamI 5438 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4606 CAATTG C^AATTG
MunI 4606 CAATTG C^AATTG
NcoI 2258 CCATGG C^CATGG
NsiI 865 ATGCAT ATGCA^T
PflFI 612 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 6903 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 6704 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2493 TCATGA T^CATGA
SacII 89 CCGCGG CCGC^GG
SapI 2342 GAAGAGC GCTCTTC(1/4)
SbfI 6802 CCTGCAGG CCTGCA^GG
ScaI 597 AGTACT AGT^ACT
SnaBI 3290 TACGTA TAC^GTA
SphI 867 GCATGC GCATG^C
Sse8387I 6802 CCTGCAGG CCTGCA^GG
SspBI 4315 TGTACA T^GTACA
SwaI 2219 ATTTAAAT ATTT^AAAT
Tth111I 612 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1086 CCATAATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 6597 GTCTAC GT^[AC][GT]AC
6661 GTAGAC
AccII 90 CGCG CG^CG
1272 CGCG
AflIII 936 ACATGT A^C[GA][CT]GT
1134 ACATGT
Alw44I 4514 GTGCAC G^TGCAC
6440 GTGCAC
ApaI 81 GGGCCC GGGCC^C
6001 GGGCCC
ApaLI 4514 GTGCAC G^TGCAC
6440 GTGCAC
AseI 1177 ATTAAT AT^TAAT
4612 ATTAAT
AsnI 1177 ATTAAT AT^TAAT
4612 ATTAAT
BsaAI 3290 TACGTA [CT]AC^GT[GA]
6545 CACGTA
BsmI 6858 GAATGC GAATGC(1/-1)
1185 GCATTC
BspLU11I 936 ACATGT A^CATGT
1134 ACATGT
BstUI 90 CGCG CG^CG
1272 CGCG
DraI 1198 TTTAAA TTT^AAA
2220 TTTAAA
DraIII 5077 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
5924 CACAAAGTG
HindIII 527 AAGCTT A^AGCTT
3805 AAGCTT
HpaI 2199 GTTAAC GTT^AAC
4359 GTTAAC
MvnI 90 CGCG CG^CG
1272 CGCG
PaeR7I 1523 CTCGAG C^TCGAG
2759 CTCGAG
PshBI 1177 ATTAAT AT^TAAT
4612 ATTAAT
SacI 131 GAGCTC GAGCT^C
3275 GAGCTC
SspI 2646 AATATT AAT^ATT
2847 AATATT
StuI 4867 AGGCCT AGG^CCT
5035 AGGCCT
XbaI 2878 TCTAGA T^CTAGA
5413 TCTAGA
XhoI 1523 CTCGAG C^TCGAG
2759 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NheI NlaIII NlaIV NspI
PleI Psp1406I PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI