(Circular) (Six-base) MAPSORT of: pFN21AB7322.seq Check: 3302 from: 1 to: 11761 pFN21AB7322 11761 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4306 5366 4306 Size: 1060 10701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9571 278 Size: 53 83 186 936 8035 2468 Fragments arranged by size: 8035 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 8603 8667 9104 1937 Size: 6666 64 437 4594 Fragments arranged by size: 6666 4594 437 64 Acc65I G'GTAC_C Cuts at: 9082 9082 Size: 11761 AclI AA'CG_TT Cuts at: 1563 3771 5991 8542 9888 10261 1563 Size: 2208 2220 2551 1346 373 3063 Fragments arranged by size: 3063 2551 2220 2208 1346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2619 3839 3906 4425 5067 5564 5622 Size: 578 409 1220 67 519 642 497 58 Cuts at: 5622 5853 7604 7815 9824 11208 11476 1632 Size: 231 1751 211 2009 1384 268 1917 Fragments arranged by size: 2009 1917 1751 1384 1220 642 578 519 497 409 268 231 211 67 58 AflII C'TTAA_G Cuts at: 820 1017 7921 820 Size: 197 6904 4660 Fragments arranged by size: 6904 4660 197 AflIII A'CryG_T Cuts at: 1203 2941 3139 10660 11637 1203 Size: 1738 198 7521 977 1327 Fragments arranged by size: 7521 1738 1327 977 198 AgeI A'CCGG_T Cuts at: 10592 10592 Size: 11761 AhdI GACnn_n'nnGTC Cuts at: 10490 10490 Size: 11761 AleI CACnn'nnGTG Cuts at: 1166 7256 1166 Size: 6090 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4962 5394 8823 1184 Size: 3778 432 3429 4122 Fragments arranged by size: 4122 3778 3429 432 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3756 3788 8527 8559 8560 8592 3756 Size: 32 4739 32 1 32 6925 Fragments arranged by size: 6925 4739 32 32 32 1 AlwNI CAG_nnn'CTG Cuts at: 2477 2597 4871 4949 5365 5498 9504 11076 2477 Size: 120 2274 78 416 133 4006 1572 3162 Fragments arranged by size: 4006 3162 2274 1572 416 133 120 78 ApaI G_GGCC'C Cuts at: 2090 8010 2090 Size: 5920 5841 ApaLI G'TGCA_C Cuts at: 6519 8445 9818 10974 6519 Size: 1926 1373 1156 7306 Fragments arranged by size: 7306 1926 1373 1156 ApoI r'AATT_y Cuts at: 1493 3200 5252 6694 7129 7822 7840 7945 8128 Size: 1707 2052 1442 435 693 18 105 183 Cuts at: 8128 9070 9296 9448 11557 1493 Size: 942 226 152 2109 1697 Fragments arranged by size: 2109 2052 1707 1697 1442 942 693 435 226 183 152 105 18 AseI AT'TA_AT Cuts at: 160 1032 3183 6618 10315 160 Size: 872 2151 3435 3697 1606 Fragments arranged by size: 3697 3435 2151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11761 AvaI C'yCGr_G Cuts at: 1870 1943 3528 4764 8434 9086 1870 Size: 73 1585 1236 3670 652 4545 Fragments arranged by size: 4545 3670 1585 1236 652 73 BamHI G'GATC_C Cuts at: 6634 9091 9458 6634 Size: 2457 367 8937 Fragments arranged by size: 8937 2457 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 3374 7087 7189 7696 Size: 325 217 627 546 1041 3713 102 507 Cuts at: 7696 8317 9022 9082 10537 618 Size: 621 705 60 1455 1842 Fragments arranged by size: 3713 1842 1455 1041 705 627 621 546 507 325 217 102 60 BanII G_rGCy'C Cuts at: 1391 2063 2090 2140 4237 4976 5284 7052 8010 Size: 672 27 50 2097 739 308 1768 958 Cuts at: 8010 9080 1391 Size: 1070 4072 Fragments arranged by size: 4072 2097 1768 1070 958 739 672 308 50 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2988 4741 5051 7268 928 Size: 971 1089 1753 310 2217 5421 Fragments arranged by size: 5421 2217 1753 1089 971 310 BbvCI CC'TCA_GC Cuts at: 6688 6688 Size: 11761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2682 2716 9952 9986 1903 Size: 34 745 34 7236 34 3678 Fragments arranged by size: 7236 3678 745 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2831 3790 8440 9653 10863 2831 Size: 959 4650 1213 1210 3729 Fragments arranged by size: 4650 3729 1213 1210 959 BclI T'GATC_A Cuts at: 1548 6627 1548 Size: 5079 6682 BfrBI ATG'CAT Cuts at: 2872 2872 Size: 11761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10372 136 Size: 107 122 71 631 605 8700 1525 Fragments arranged by size: 8700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3868 11756 3868 Size: 7888 3873 BlpI GC'TnA_GC Cuts at: 9178 9178 Size: 11761 Bme1580I G_kGCm'C Cuts at: 1790 2090 3217 3379 6523 7993 8010 8320 8449 Size: 300 1127 162 3144 1470 17 310 129 Cuts at: 8449 9025 9822 10978 1790 Size: 576 797 1156 2573 Fragments arranged by size: 3144 2573 1470 1156 1127 797 576 310 300 162 129 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4678 6851 7706 Size: 416 53 83 399 24 3261 2173 855 Cuts at: 7706 9492 10450 442 Size: 1786 958 1753 Fragments arranged by size: 3261 2173 1786 1753 958 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2969 3480 5330 1056 Size: 1913 511 1850 7487 Fragments arranged by size: 7487 1913 1850 511 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4897 8214 4897 Size: 3317 8444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3054 5178 5967 6576 7671 8151 8862 8925 Size: 1815 2124 789 609 1095 480 711 63 Cuts at: 8925 10421 1239 Size: 1496 2579 Fragments arranged by size: 2579 2124 1815 1496 1095 789 711 609 480 63 Bpu10I CC'TnA_GC Cuts at: 3223 6381 6688 7074 3223 Size: 3158 307 386 7910 Fragments arranged by size: 7910 3158 386 307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3588 3705 5191 5781 9886 10751 11049 3588 Size: 117 1486 590 4105 865 298 4300 Fragments arranged by size: 4300 4105 1486 865 590 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5111 6552 8185 10424 882 Size: 617 3612 1441 1633 2239 2219 Fragments arranged by size: 3612 2239 2219 1633 1441 617 BsaAI yAC'GTr Cuts at: 493 1204 5297 8552 9552 493 Size: 711 4093 3255 1000 2702 Fragments arranged by size: 4093 3255 2702 1000 711 BsaBI GATnn'nnATC Cuts at: 7447 11755 7447 Size: 4308 7453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 9568 9950 10585 275 Size: 53 83 186 936 8035 382 635 1451 Fragments arranged by size: 8035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2023 2235 3071 6561 10194 10592 10866 11013 11424 Size: 212 836 3490 3633 398 274 147 411 Cuts at: 11424 2023 Size: 2360 Fragments arranged by size: 3633 3490 2360 836 411 398 274 212 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2928 2958 3027 3057 3756 Size: 30 165 30 1525 30 69 30 699 Cuts at: 3756 3786 3954 3984 4402 4432 4742 4772 5833 Size: 30 168 30 418 30 310 30 1061 Cuts at: 5833 5863 7510 7540 7648 7678 8052 8082 8560 Size: 30 1647 30 108 30 374 30 478 Cuts at: 8560 8590 1178 Size: 30 4349 Fragments arranged by size: 4349 1647 1525 1061 699 478 418 374 310 168 165 108 69 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2145 2399 3486 4227 4581 4902 5194 5406 Size: 959 254 1087 741 354 321 292 212 Cuts at: 5406 5648 6495 6676 6900 8343 1186 Size: 242 847 181 224 1443 4604 Fragments arranged by size: 4604 1443 1087 959 847 741 354 321 292 254 242 224 212 181 BseYI C'CCAG_C Cuts at: 2252 7401 7752 8492 10964 11460 2252 Size: 5149 351 740 2472 496 2553 Fragments arranged by size: 5149 2553 2472 740 496 351 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 2640 4041 4689 5400 7302 8403 Size: 573 772 132 1401 648 711 1902 1101 Cuts at: 8403 8532 1163 Size: 129 4392 Fragments arranged by size: 4392 1902 1401 1101 772 711 648 573 132 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9211 9972 11000 1540 Size: 275 186 7210 761 1028 2301 Fragments arranged by size: 7210 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 3063 4584 4785 5284 6523 8335 8449 9822 Size: 923 1521 201 499 1239 1812 114 1373 Cuts at: 9822 9907 10978 2140 Size: 85 1071 2923 Fragments arranged by size: 2923 1812 1521 1373 1239 1071 923 499 201 114 85 BsmI GAATG_Cn' Cuts at: 3190 8869 9272 9365 3190 Size: 5679 403 93 5586 Fragments arranged by size: 5679 5586 403 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2157 2157 Size: 11761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2090 2140 3063 3217 3379 4237 Size: 399 273 27 50 923 154 162 858 Cuts at: 4237 4584 4785 4976 5284 6523 7052 7993 8010 Size: 347 201 191 308 1239 529 941 17 Cuts at: 8010 8320 8335 8449 9025 9080 9822 9907 10978 Size: 310 15 114 576 55 742 85 1071 Cuts at: 10978 1391 Size: 2174 Fragments arranged by size: 2174 1239 1071 941 923 858 742 576 529 399 347 310 308 273 201 191 162 154 114 85 55 50 27 17 15 BspEI T'CCGG_A Cuts at: 11424 11424 Size: 11761 BspHI T'CATG_A Cuts at: 4498 9650 11380 4498 Size: 5152 1730 4879 Fragments arranged by size: 5152 4879 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 4306 5366 5897 7127 7478 8203 9116 Size: 1346 2116 1060 531 1230 351 725 913 Cuts at: 9116 844 Size: 3489 Fragments arranged by size: 3489 2116 1346 1230 1060 913 725 531 351 BsrBI CCG'CTC Cuts at: 1394 1823 9648 1394 Size: 429 7825 3507 Fragments arranged by size: 7825 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 4365 8503 8754 8874 10256 10430 Size: 1183 1626 1491 4138 251 120 1382 174 Cuts at: 10430 65 Size: 1396 Fragments arranged by size: 4138 1626 1491 1396 1382 1183 251 174 120 BsrFI r'CCGG_y Cuts at: 1700 1810 10405 10592 1700 Size: 110 8595 187 2869 Fragments arranged by size: 8595 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 6320 96 Size: 6224 5537 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11761 BssSI C'ACGA_G Cuts at: 2157 4239 4535 9821 10833 2157 Size: 2082 296 5286 1012 3085 Fragments arranged by size: 5286 3085 2082 1012 296 BstAPI GCAn_nnn'nTGC Cuts at: 794 2873 794 Size: 2079 9682 BstBI TT'CG_AA Cuts at: 10565 10565 Size: 11761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11761 BstYI r'GATC_y Cuts at: 1972 2506 2593 3868 4069 6634 6957 7589 8063 Size: 534 87 1275 201 2565 323 632 474 Cuts at: 8063 8134 9091 9458 9843 9860 11312 11398 11410 Size: 71 957 367 385 17 1452 86 12 Cuts at: 11410 11756 1972 Size: 346 1977 Fragments arranged by size: 2565 1977 1452 1275 957 632 534 474 385 367 346 323 201 87 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2495 4278 8209 2495 Size: 1783 3931 6047 Fragments arranged by size: 6047 3931 1783 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2568 4263 7296 8979 513 Size: 552 939 90 474 1695 3033 1683 3295 Fragments arranged by size: 3295 3033 1695 1683 939 552 474 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6135 487 Size: 683 56 4909 6113 Fragments arranged by size: 6113 4909 683 56 BtsI GCAGTG_nn' Cuts at: 3309 4044 4256 4553 5010 6726 7120 7314 9277 Size: 735 212 297 457 1716 394 194 1963 Cuts at: 9277 10071 10091 3309 Size: 794 20 4979 Fragments arranged by size: 4979 1963 1716 794 735 457 394 297 212 194 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4596 4631 4698 4733 6954 6989 7443 Size: 35 4032 35 67 35 2221 35 454 Cuts at: 7443 7478 8987 9022 529 Size: 35 1509 35 3268 Fragments arranged by size: 4032 3268 2221 1509 454 67 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 3205 4227 9065 3205 Size: 1022 4838 5901 Fragments arranged by size: 5901 4838 1022 DraIII CAC_nnn'GTG Cuts at: 7087 7934 7087 Size: 847 10914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10768 809 Size: 507 9452 1802 Fragments arranged by size: 9452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3337 4126 4531 Size: 54 1425 50 275 18 1507 789 405 Cuts at: 4531 9208 10097 11726 8 Size: 4677 889 1629 43 Fragments arranged by size: 4677 1629 1507 1425 889 789 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9208 1537 Size: 275 7396 4090 Fragments arranged by size: 7396 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2376 3480 4323 4342 5705 6231 6472 Size: 1002 21 1104 843 19 1363 526 241 Cuts at: 6472 6897 7623 7903 9691 1353 Size: 425 726 280 1788 3423 Fragments arranged by size: 3423 1788 1363 1104 1002 843 726 526 425 280 241 21 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2331 10348 10722 10868 387 Size: 1064 138 528 214 8017 374 146 1280 Fragments arranged by size: 8017 1280 1064 528 374 214 146 138 EcoICRI GAG'CTC Cuts at: 2138 5282 2138 Size: 3144 8617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2619 3054 3839 3906 4425 5067 Size: 393 578 409 435 785 67 519 642 Cuts at: 5067 5178 5564 5622 5853 5967 6576 7604 7671 Size: 111 386 58 231 114 609 1028 67 Cuts at: 7671 7815 8151 8862 8925 9824 10421 11208 11476 Size: 144 336 711 63 899 597 787 268 Cuts at: 11476 1239 Size: 1524 Fragments arranged by size: 1524 1028 899 787 785 711 642 609 597 578 519 435 409 393 386 336 268 231 144 114 111 67 67 63 58 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 11761 EcoO109I rG'GnC_Cy Cuts at: 2087 8007 8909 2087 Size: 5920 902 4939 Fragments arranged by size: 5920 4939 902 EcoRI G'AATT_C Cuts at: 7129 7822 7945 9070 9448 7129 Size: 693 123 1125 378 9442 Fragments arranged by size: 9442 1125 693 378 123 EcoRV GAT'ATC Cuts at: 6462 6462 Size: 11761 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 3079 3918 5828 7242 2482 Size: 597 839 1910 1414 7001 Fragments arranged by size: 7001 1910 1414 839 597 FspI TGC'GCA Cuts at: 10267 10267 Size: 11761 HaeII r_GCGC'y Cuts at: 1678 4511 10908 1678 Size: 2833 6397 2531 Fragments arranged by size: 6397 2833 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 2491 2523 2928 Size: 32 135 32 815 32 72 32 405 Cuts at: 2928 2960 3564 3596 4402 4434 4958 4990 5401 Size: 32 604 32 806 32 524 32 411 Cuts at: 5401 5433 5833 5865 6145 6177 6230 6262 6619 Size: 32 400 32 280 32 53 32 357 Cuts at: 6619 6651 7427 7459 7648 7680 8677 8709 10402 Size: 32 776 32 189 32 997 32 1693 Cuts at: 10402 10434 10476 10508 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1693 997 815 806 776 604 524 411 405 400 357 280 189 135 72 53 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2616 4206 6366 8085 8385 9105 Size: 988 281 678 1590 2160 1719 300 720 Cuts at: 9105 9351 669 Size: 246 3079 Fragments arranged by size: 3079 2160 1719 1590 988 720 678 300 281 246 HindIII A'AGCT_T Cuts at: 748 2532 5810 748 Size: 1784 3278 6699 Fragments arranged by size: 6699 3278 1784 HpaI GTT'AAC Cuts at: 4206 6366 9351 4206 Size: 2160 2985 6616 Fragments arranged by size: 6616 2985 2160 KpnI G_GTAC'C Cuts at: 9086 9086 Size: 11761 MfeI C'AATT_G Cuts at: 6611 9360 6611 Size: 2749 9012 MluI A'CGCG_T Cuts at: 11637 11637 Size: 11761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 2682 4439 5648 10875 11059 1305 Size: 1314 63 1757 1209 5227 184 2007 Fragments arranged by size: 5227 2007 1757 1314 1209 184 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 3339 4128 4533 10 Size: 54 1425 343 1507 789 405 7238 Fragments arranged by size: 7238 1507 1425 789 405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2877 3195 3834 4134 4638 4788 5289 Size: 648 1711 318 639 300 504 150 501 Cuts at: 5289 5349 7256 8799 9719 10078 10237 518 Size: 60 1907 1543 920 359 159 2042 Fragments arranged by size: 2042 1907 1711 1543 920 648 639 504 501 359 318 300 159 150 60 MspA1I CmG'CkG Cuts at: 1606 2096 2289 3372 4628 4946 6114 6173 8342 Size: 490 193 1083 1256 318 1168 59 2169 Cuts at: 8342 9177 9854 11002 11247 1606 Size: 835 677 1148 245 2120 Fragments arranged by size: 2169 2120 1256 1168 1148 1083 835 677 490 318 245 193 59 NcoI C'CATG_G Cuts at: 513 1065 2004 4263 513 Size: 552 939 2259 8011 Fragments arranged by size: 8011 2259 939 552 NdeI CA'TA_TG Cuts at: 387 3022 3811 8236 9045 387 Size: 2635 789 4425 809 3103 Fragments arranged by size: 4425 3103 2635 809 789 NheI G'CTAG_C Cuts at: 1052 2965 3476 5326 1052 Size: 1913 511 1850 7487 Fragments arranged by size: 7487 1913 1850 511 NotI GC'GGCC_GC Cuts at: 9208 9208 Size: 11761 NsiI A_TGCA'T Cuts at: 2874 2874 Size: 11761 NspI r_CATG'y Cuts at: 1132 1229 2876 2945 3143 3391 3432 3443 6929 Size: 97 1647 69 198 248 41 11 3486 Cuts at: 6929 8804 9119 10664 1132 Size: 1875 315 1545 2229 Fragments arranged by size: 3486 2229 1875 1647 1545 315 248 198 97 69 41 11 PasI CC'CwG_GG Cuts at: 1348 8681 9018 1348 Size: 7333 337 4091 Fragments arranged by size: 7333 4091 337 PciI A'CATG_T Cuts at: 2941 3139 10660 2941 Size: 198 7521 4042 Fragments arranged by size: 7521 4042 198 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 11761 PfoI T'CCnGG_A Cuts at: 7585 7585 Size: 11761 PmeI GTTT'AAAC Cuts at: 9065 9065 Size: 11761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3756 3788 8560 8592 9830 9862 11343 11375 3756 Size: 32 4772 32 1238 32 1481 32 4142 Fragments arranged by size: 4772 4142 1481 1238 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 8909 8909 Size: 11761 PshAI GACnn'nnGTC Cuts at: 1613 8713 1613 Size: 7100 4661 PsiI TTA'TAA Cuts at: 9331 9331 Size: 11761 PspOMI G'GGCC_C Cuts at: 2086 8006 2086 Size: 5920 5841 PspXI vC'TCGA_Gb Cuts at: 4764 4764 Size: 11761 PstI C_TGCA'G Cuts at: 830 2047 2551 3763 6119 8812 8938 9113 830 Size: 1217 504 1212 2356 2693 126 175 3478 Fragments arranged by size: 3478 2693 2356 1217 1212 504 175 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11761 PvuII CAG'CTG Cuts at: 4628 4946 6114 6173 8342 4628 Size: 318 1168 59 2169 8047 Fragments arranged by size: 8047 2169 1168 318 59 SacI G_AGCT'C Cuts at: 2140 5284 2140 Size: 3144 8617 SacII CC_GC'GG Cuts at: 2097 2097 Size: 11761 SalI G'TCGA_C Cuts at: 1936 9103 1936 Size: 7167 4594 SapI GCTCTTCn'nnn_ Cuts at: 4342 4342 Size: 11761 SbfI CC_TGCA'GG Cuts at: 8812 9113 8812 Size: 301 11460 ScaI AGT'ACT Cuts at: 2604 10009 11721 2604 Size: 7405 1712 2644 Fragments arranged by size: 7405 2644 1712 SfcI C'TryA_G Cuts at: 826 1045 2043 2547 3759 6115 6768 8808 8934 Size: 219 998 504 1212 2356 653 2040 126 Cuts at: 8934 9109 10244 10925 11116 826 Size: 175 1135 681 191 1471 Fragments arranged by size: 2356 2040 1471 1212 1135 998 681 653 504 219 191 175 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11761 SmaI CCC'GGG Cuts at: 1872 9088 1872 Size: 7216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3528 3603 3720 4764 5206 5796 Size: 197 926 1585 75 117 1044 442 590 Cuts at: 5796 7921 9865 10766 11028 820 Size: 2125 1944 901 262 1553 Fragments arranged by size: 2125 1944 1585 1553 1044 926 901 590 442 262 197 117 75 SnaBI TAC'GTA Cuts at: 493 5297 9552 493 Size: 4804 4255 2702 Fragments arranged by size: 4804 4255 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11761 SphI G_CATG'C Cuts at: 1132 2876 9119 1132 Size: 1744 6243 3774 Fragments arranged by size: 6243 3774 1744 SspI AAT'ATT Cuts at: 5 52 4653 4854 9685 5 Size: 47 4601 201 4831 2081 Fragments arranged by size: 4831 4601 2081 201 47 StuI AGG'CCT Cuts at: 1517 6874 7042 1517 Size: 5357 168 6236 Fragments arranged by size: 6236 5357 168 StyI C'CwwG_G Cuts at: 513 1065 2004 4263 4698 4716 5035 6968 8323 Size: 552 939 2259 435 18 319 1933 1355 Cuts at: 8323 9200 513 Size: 877 3074 Fragments arranged by size: 3074 2259 1933 1355 939 877 552 435 319 18 SwaI ATTT'AAAT Cuts at: 4227 4227 Size: 11761 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7336 8725 9788 9805 9958 10143 10588 Size: 1255 5756 1389 1063 17 153 185 445 Cuts at: 10588 325 Size: 1498 Fragments arranged by size: 5756 1498 1389 1255 1063 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2602 3935 4003 Size: 274 80 33 51 1443 625 1333 68 Cuts at: 4003 5575 6320 7925 7951 8762 10007 11719 96 Size: 1572 745 1605 26 811 1245 1712 138 Fragments arranged by size: 1712 1605 1572 1443 1333 1245 811 745 625 274 138 80 68 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3954 3986 4303 4335 7009 7041 7215 7247 7510 Size: 32 317 32 2674 32 174 32 263 Cuts at: 7510 7542 11721 11753 3954 Size: 32 4179 32 3962 Fragments arranged by size: 4179 3962 2674 317 263 174 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2620 1316 Size: 1304 10457 XbaI T'CTAG_A Cuts at: 4883 7418 9097 4883 Size: 2535 1679 7547 Fragments arranged by size: 7547 2535 1679 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3098 3098 Size: 11761 XhoI C'TCGA_G Cuts at: 1943 3528 4764 1943 Size: 1585 1236 8940 Fragments arranged by size: 8940 1585 1236 XmaI C'CCGG_G Cuts at: 1870 9086 1870 Size: 7216 4545 XmnI GAAnn'nnTTC Cuts at: 2604 5991 6660 9890 2604 Size: 3387 669 3230 4475 Fragments arranged by size: 4475 3387 3230 669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9569 276 Size: 53 83 186 936 8035 2468 Fragments arranged by size: 8035 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SanDI SexAI SfiI SfoI SrfI