Restriction map for
pFN21AB7322
(Linear) (Six-base) MAPSORT of: KIBB7322.seq Check: 8025 from: 1 to: 7056
KIBB7322.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2301 3361 7056
Size: 2301 1060 3695
Fragments arranged by size:
3695 2301 1060
AccI GT'mk_AC
Cuts at: 0 6598 6662 7056
Size: 6598 64 394
Fragments arranged by size:
6598 394 64
AclI AA'CG_TT
Cuts at: 0 1766 3986 6537 7056
Size: 1766 2220 2551 519
Fragments arranged by size:
2551 2220 1766 519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 614 1834 1901 2420 3062 3559 3617
Size: 205 409 1220 67 519 642 497 58
Cuts at: 3617 3848 5599 5810 7056
Size: 231 1751 211 1246
Fragments arranged by size:
1751 1246 1220 642 519 497 409 231
211 205 67 58
AflII C'TTAA_G
Cuts at: 0 5916 7056
Size: 5916 1140
AflIII A'CryG_T
Cuts at: 0 936 1134 7056
Size: 936 198 5922
Fragments arranged by size:
5922 936 198
AleI CACnn'nnGTG
Cuts at: 0 5251 7056
Size: 5251 1805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2957 3389 6818 7056
Size: 2957 432 3429 238
Fragments arranged by size:
3429 2957 432 238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1751 1783 6522 6554 6555 6587 7056
Size: 1751 32 4739 32 1 32 469
Fragments arranged by size:
4739 1751 469 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 0 472 592 2866 2944 3360 3493 7056
Size: 472 120 2274 78 416 133 3563
Fragments arranged by size:
3563 2274 472 416 133 120 78
ApaI G_GGCC'C
Cuts at: 0 85 6005 7056
Size: 85 5920 1051
Fragments arranged by size:
5920 1051 85
ApaLI G'TGCA_C
Cuts at: 0 4514 6440 7056
Size: 4514 1926 616
Fragments arranged by size:
4514 1926 616
ApoI r'AATT_y
Cuts at: 0 1195 3247 4689 5124 5817 5835 5940 6123
Size: 1195 2052 1442 435 693 18 105 183
Cuts at: 6123 7056
Size: 933
Fragments arranged by size:
2052 1442 1195 933 693 435 183 105
18
AseI AT'TA_AT
Cuts at: 0 1178 4613 7056
Size: 1178 3435 2443
Fragments arranged by size:
3435 2443 1178
AvaI C'yCGr_G
Cuts at: 0 1523 2759 6429 7056
Size: 1523 1236 3670 627
Fragments arranged by size:
3670 1523 1236 627
BamHI G'GATC_C
Cuts at: 0 4629 7056
Size: 4629 2427
BanI G'GyrC_C
Cuts at: 0 328 1369 5082 5184 5691 6312 7017 7056
Size: 328 1041 3713 102 507 621 705 39
Fragments arranged by size:
3713 1041 705 621 507 328 102 39
BanII G_rGCy'C
Cuts at: 0 58 85 135 2232 2971 3279 5047 6005
Size: 58 27 50 2097 739 308 1768 958
Cuts at: 6005 7056
Size: 1051
Fragments arranged by size:
2097 1768 1051 958 739 308 58 50
27
BbsI GAAGACnn'nnnn_
Cuts at: 0 983 2736 3046 5263 7056
Size: 983 1753 310 2217 1793
Fragments arranged by size:
2217 1793 1753 983 310
BbvCI CC'TCA_GC
Cuts at: 0 4683 7056
Size: 4683 2373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 677 711 7056
Size: 677 34 6345
Fragments arranged by size:
6345 677 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 826 1785 6435 7056
Size: 826 959 4650 621
Fragments arranged by size:
4650 959 826 621
BclI T'GATC_A
Cuts at: 0 4622 7056
Size: 4622 2434
BfrBI ATG'CAT
Cuts at: 0 867 7056
Size: 867 6189
BglII A'GATC_T
Cuts at: 0 1863 7056
Size: 1863 5193
Bme1580I G_kGCm'C
Cuts at: 0 85 1212 1374 4518 5988 6005 6315 6444
Size: 85 1127 162 3144 1470 17 310 129
Cuts at: 6444 7020 7056
Size: 576 36
Fragments arranged by size:
3144 1470 1127 576 310 162 129 85
36 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 2673 4846 5701 7056
Size: 2673 2173 855 1355
Fragments arranged by size:
2673 2173 1355 855
BmtI G_CTAG'C
Cuts at: 0 964 1475 3325 7056
Size: 964 511 1850 3731
Fragments arranged by size:
3731 1850 964 511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2892 6209 7056
Size: 2892 3317 847
Fragments arranged by size:
3317 2892 847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 3173 3962 4571 5666 6146 6857 6920
Size: 1049 2124 789 609 1095 480 711 63
Cuts at: 6920 7056
Size: 136
Fragments arranged by size:
2124 1095 1049 789 711 609 480 136
63
Bpu10I CC'TnA_GC
Cuts at: 0 1218 4376 4683 5069 7056
Size: 1218 3158 307 386 1987
Fragments arranged by size:
3158 1987 1218 386 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1583 1700 3186 3776 7056
Size: 1583 117 1486 590 3280
Fragments arranged by size:
3280 1583 1486 590 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 3106 4547 6180 7056
Size: 3106 1441 1633 876
Fragments arranged by size:
3106 1633 1441 876
BsaAI yAC'GTr
Cuts at: 0 3292 6547 7056
Size: 3292 3255 509
Fragments arranged by size:
3292 3255 509
BsaBI GATnn'nnATC
Cuts at: 0 5442 7056
Size: 5442 1614
BsaWI w'CCGG_w
Cuts at: 0 18 230 1066 4556 7056
Size: 18 212 836 3490 2500
Fragments arranged by size:
3490 2500 836 212 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 923 953 1022 1052 1751 1781 1949 1979
Size: 923 30 69 30 699 30 168 30
Cuts at: 1979 2397 2427 2737 2767 3828 3858 5505 5535
Size: 418 30 310 30 1061 30 1647 30
Cuts at: 5535 5643 5673 6047 6077 6555 6585 7056
Size: 108 30 374 30 478 30 471
Fragments arranged by size:
1647 1061 923 699 478 471 418 374
310 168 108 69 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 140 394 1481 2222 2576 2897 3189 3401
Size: 140 254 1087 741 354 321 292 212
Cuts at: 3401 3643 4490 4671 4895 6338 7056
Size: 242 847 181 224 1443 718
Fragments arranged by size:
1443 1087 847 741 718 354 321 292
254 242 224 212 181 140
BseYI C'CCAG_C
Cuts at: 0 247 5396 5747 6487 7056
Size: 247 5149 351 740 569
Fragments arranged by size:
5149 740 569 351 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 635 2036 2684 3395 5297 6398 6527
Size: 503 132 1401 648 711 1902 1101 129
Cuts at: 6527 7056
Size: 529
Fragments arranged by size:
1902 1401 1101 711 648 529 503 132
129
BsiHKAI G_wGCw'C
Cuts at: 0 135 1058 2579 2780 3279 4518 6330 6444
Size: 135 923 1521 201 499 1239 1812 114
Cuts at: 6444 7056
Size: 612
Fragments arranged by size:
1812 1521 1239 923 612 499 201 135
114
BsmI GAATG_Cn'
Cuts at: 0 1185 6864 7056
Size: 1185 5679 192
Fragments arranged by size:
5679 1185 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 152 7056
Size: 152 6904
Bsp1286I G_dGCh'C
Cuts at: 0 58 85 135 1058 1212 1374 2232 2579
Size: 58 27 50 923 154 162 858 347
Cuts at: 2579 2780 2971 3279 4518 5047 5988 6005 6315
Size: 201 191 308 1239 529 941 17 310
Cuts at: 6315 6330 6444 7020 7056
Size: 15 114 576 36
Fragments arranged by size:
1239 941 923 858 576 529 347 310
308 201 191 162 154 114 58 50
36 27 17 15
BspHI T'CATG_A
Cuts at: 0 2493 7056
Size: 2493 4563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 2301 3361 3892 5122 5473 6198 7056
Size: 185 2116 1060 531 1230 351 725 858
Fragments arranged by size:
2116 1230 1060 858 725 531 351 185
BsrDI GCAATG_nn'
Cuts at: 0 869 2360 6498 6749 6869 7056
Size: 869 1491 4138 251 120 187
Fragments arranged by size:
4138 1491 869 251 187 120
BsrGI T'GTAC_A
Cuts at: 0 4315 7056
Size: 4315 2741
BssSI C'ACGA_G
Cuts at: 0 152 2234 2530 7056
Size: 152 2082 296 4526
Fragments arranged by size:
4526 2082 296 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 868 7056
Size: 868 6188
BstYI r'GATC_y
Cuts at: 0 501 588 1863 2064 4629 4952 5584 6058
Size: 501 87 1275 201 2565 323 632 474
Cuts at: 6058 6129 7056
Size: 71 927
Fragments arranged by size:
2565 1275 927 632 501 474 323 201
87 71
Bsu36I CC'TnA_GG
Cuts at: 0 490 2273 6204 7056
Size: 490 1783 3931 852
Fragments arranged by size:
3931 1783 852 490
BtgI C'CryG_G
Cuts at: 0 89 563 2258 5291 6974 7056
Size: 89 474 1695 3033 1683 82
Fragments arranged by size:
3033 1695 1683 474 89 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4130 7056
Size: 4130 2926
BtsI GCAGTG_nn'
Cuts at: 0 1304 2039 2251 2548 3005 4721 5115 5309
Size: 1304 735 212 297 457 1716 394 194
Cuts at: 5309 7056
Size: 1747
Fragments arranged by size:
1747 1716 1304 735 457 394 297 212
194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2591 2626 2693 2728 4949 4984 5438 5473
Size: 2591 35 67 35 2221 35 454 35
Cuts at: 5473 6982 7017 7056
Size: 1509 35 39
Fragments arranged by size:
2591 2221 1509 454 67 39 35 35
35 35 35
DraI TTT'AAA
Cuts at: 0 1200 2222 7056
Size: 1200 1022 4834
Fragments arranged by size:
4834 1200 1022
DraIII CAC_nnn'GTG
Cuts at: 0 5082 5929 7056
Size: 5082 847 1127
Fragments arranged by size:
5082 1127 847
EaeI y'GGCC_r
Cuts at: 0 1332 2121 2526 7056
Size: 1332 789 405 4530
Fragments arranged by size:
4530 1332 789 405
EarI CTCTTCn'nnn_
Cuts at: 0 350 371 1475 2318 2337 3700 4226 4467
Size: 350 21 1104 843 19 1363 526 241
Cuts at: 4467 4892 5618 5898 7056
Size: 425 726 280 1158
Fragments arranged by size:
1363 1158 1104 843 726 526 425 350
280 241 21 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 326 7056
Size: 112 214 6730
Fragments arranged by size:
6730 214 112
EcoICRI GAG'CTC
Cuts at: 0 133 3277 7056
Size: 133 3144 3779
Fragments arranged by size:
3779 3144 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 614 1049 1834 1901 2420 3062 3173
Size: 205 409 435 785 67 519 642 111
Cuts at: 3173 3559 3617 3848 3962 4571 5599 5666 5810
Size: 386 58 231 114 609 1028 67 144
Cuts at: 5810 6146 6857 6920 7056
Size: 336 711 63 136
Fragments arranged by size:
1028 785 711 642 609 519 435 409
386 336 231 205 144 136 114 111
67 67 63 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 7056
Size: 1480 5576
EcoO109I rG'GnC_Cy
Cuts at: 0 82 6002 6904 7056
Size: 82 5920 902 152
Fragments arranged by size:
5920 902 152 82
EcoRI G'AATT_C
Cuts at: 0 5124 5817 5940 7056
Size: 5124 693 123 1116
Fragments arranged by size:
5124 1116 693 123
EcoRV GAT'ATC
Cuts at: 0 4457 7056
Size: 4457 2599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 1074 1913 3823 5237 7056
Size: 477 597 839 1910 1414 1819
Fragments arranged by size:
1910 1819 1414 839 597 477
HaeII r_GCGC'y
Cuts at: 0 2506 7056
Size: 2506 4550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 486 518 923 955 1559 1591
Size: 382 32 72 32 405 32 604 32
Cuts at: 1591 2397 2429 2953 2985 3396 3428 3828 3860
Size: 806 32 524 32 411 32 400 32
Cuts at: 3860 4140 4172 4225 4257 4614 4646 5422 5454
Size: 280 32 53 32 357 32 776 32
Cuts at: 5454 5643 5675 6672 6704 7056
Size: 189 32 997 32 352
Fragments arranged by size:
997 806 776 604 524 411 405 400
382 357 352 280 189 72 53 32
32 32 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 611 2201 4361 6080 6380 7056
Size: 611 1590 2160 1719 300 676
Fragments arranged by size:
2160 1719 1590 676 611 300
HindIII A'AGCT_T
Cuts at: 0 527 3805 7056
Size: 527 3278 3251
Fragments arranged by size:
3278 3251 527
HpaI GTT'AAC
Cuts at: 0 2201 4361 7056
Size: 2201 2160 2695
Fragments arranged by size:
2695 2201 2160
MfeI C'AATT_G
Cuts at: 0 4606 7056
Size: 4606 2450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 677 2434 3643 7056
Size: 614 63 1757 1209 3413
Fragments arranged by size:
3413 1757 1209 614 63
MscI TGG'CCA
Cuts at: 0 1334 2123 2528 7056
Size: 1334 789 405 4528
Fragments arranged by size:
4528 1334 789 405
MslI CAynn'nnrTG
Cuts at: 0 872 1190 1829 2129 2633 2783 3284 3344
Size: 872 318 639 300 504 150 501 60
Cuts at: 3344 5251 6794 7056
Size: 1907 1543 262
Fragments arranged by size:
1907 1543 872 639 504 501 318 300
262 150 60
MspA1I CmG'CkG
Cuts at: 0 91 284 1367 2623 2941 4109 4168 6337
Size: 91 193 1083 1256 318 1168 59 2169
Cuts at: 6337 7056
Size: 719
Fragments arranged by size:
2169 1256 1168 1083 719 318 193 91
59
NcoI C'CATG_G
Cuts at: 0 2258 7056
Size: 2258 4798
NdeI CA'TA_TG
Cuts at: 0 1017 1806 6231 7040 7056
Size: 1017 789 4425 809 16
Fragments arranged by size:
4425 1017 809 789 16
NheI G'CTAG_C
Cuts at: 0 960 1471 3321 7056
Size: 960 511 1850 3735
Fragments arranged by size:
3735 1850 960 511
NsiI A_TGCA'T
Cuts at: 0 869 7056
Size: 869 6187
NspI r_CATG'y
Cuts at: 0 871 940 1138 1386 1427 1438 4924 6799
Size: 871 69 198 248 41 11 3486 1875
Cuts at: 6799 7056
Size: 257
Fragments arranged by size:
3486 1875 871 257 248 198 69 41
11
PasI CC'CwG_GG
Cuts at: 0 6676 7013 7056
Size: 6676 337 43
Fragments arranged by size:
6676 337 43
PciI A'CATG_T
Cuts at: 0 936 1134 7056
Size: 936 198 5922
Fragments arranged by size:
5922 936 198
PfoI T'CCnGG_A
Cuts at: 0 5580 7056
Size: 5580 1476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1751 1783 6555 6587 7056
Size: 1751 32 4772 32 469
Fragments arranged by size:
4772 1751 469 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 6904 7056
Size: 6904 152
PshAI GACnn'nnGTC
Cuts at: 0 6708 7056
Size: 6708 348
PspOMI G'GGCC_C
Cuts at: 0 81 6001 7056
Size: 81 5920 1055
Fragments arranged by size:
5920 1055 81
PspXI vC'TCGA_Gb
Cuts at: 0 2759 7056
Size: 2759 4297
PstI C_TGCA'G
Cuts at: 0 42 546 1758 4114 6807 6933 7056
Size: 42 504 1212 2356 2693 126 123
Fragments arranged by size:
2693 2356 1212 504 126 123 42
PvuII CAG'CTG
Cuts at: 0 2623 2941 4109 4168 6337 7056
Size: 2623 318 1168 59 2169 719
Fragments arranged by size:
2623 2169 1168 719 318 59
SacI G_AGCT'C
Cuts at: 0 135 3279 7056
Size: 135 3144 3777
Fragments arranged by size:
3777 3144 135
SacII CC_GC'GG
Cuts at: 0 92 7056
Size: 92 6964
SapI GCTCTTCn'nnn_
Cuts at: 0 2337 7056
Size: 2337 4719
SbfI CC_TGCA'GG
Cuts at: 0 6807 7056
Size: 6807 249
ScaI AGT'ACT
Cuts at: 0 599 7056
Size: 599 6457
SfcI C'TryA_G
Cuts at: 0 38 542 1754 4110 4763 6803 6929 7056
Size: 38 504 1212 2356 653 2040 126 127
Fragments arranged by size:
2356 2040 1212 653 504 127 126 38
SmlI C'TyrA_G
Cuts at: 0 1523 1598 1715 2759 3201 3791 5916 7056
Size: 1523 75 117 1044 442 590 2125 1140
Fragments arranged by size:
2125 1523 1140 1044 590 442 117 75
SnaBI TAC'GTA
Cuts at: 0 3292 7056
Size: 3292 3764
SphI G_CATG'C
Cuts at: 0 871 7056
Size: 871 6185
SspI AAT'ATT
Cuts at: 0 2648 2849 7056
Size: 2648 201 4207
Fragments arranged by size:
4207 2648 201
StuI AGG'CCT
Cuts at: 0 4869 5037 7056
Size: 4869 168 2019
Fragments arranged by size:
4869 2019 168
StyI C'CwwG_G
Cuts at: 0 2258 2693 2711 3030 4963 6318 7056
Size: 2258 435 18 319 1933 1355 738
Fragments arranged by size:
2258 1933 1355 738 435 319 18
SwaI ATTT'AAAT
Cuts at: 0 2222 7056
Size: 2222 4834
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 5331 6720 7056
Size: 5331 1389 336
Fragments arranged by size:
5331 1389 336
TatI w'GTAC_w
Cuts at: 0 597 1930 1998 3570 4315 5920 5946 6757
Size: 597 1333 68 1572 745 1605 26 811
Cuts at: 6757 7056
Size: 299
Fragments arranged by size:
1605 1572 1333 811 745 597 299 68
26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1949 1981 2298 2330 5004 5036 5210 5242
Size: 1949 32 317 32 2674 32 174 32
Cuts at: 5242 5505 5537 7056
Size: 263 32 1519
Fragments arranged by size:
2674 1949 1519 317 263 174 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 615 7056
Size: 615 6441
XbaI T'CTAG_A
Cuts at: 0 2878 5413 7056
Size: 2878 2535 1643
Fragments arranged by size:
2878 2535 1643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1093 7056
Size: 1093 5963
XhoI C'TCGA_G
Cuts at: 0 1523 2759 7056
Size: 1523 1236 4297
Fragments arranged by size:
4297 1523 1236
XmnI GAAnn'nnTTC
Cuts at: 0 599 3986 4655 7056
Size: 599 3387 669 2401
Fragments arranged by size:
3387 2401 669 599
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI
BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PasI PciI PfoI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SacII SapI
SbfI ScaI SfcI SmlI SnaBI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AscI AsiSI AvrII
BaeI BbeI BglI BlpI BmgBI BsaHI BsiEI BsiWI
BspEI BsrBI BsrFI BssHII BstBI BstEII BstXI BstZ17I
ClaI DrdI EagI FseI FspI FspAI KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI PacI PflMI
PmeI PmlI PsiI PsrI PvuI RsrII SalI SanDI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
XmaI ZraI