Restriction map for
pFN21AA0708
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# NO CUT ENZYMES ( 51 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
BciVI BfrI BsaBI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DrdI
EcoO65I FseI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI SacII SfiI
SfuI SnaBI SpeI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 6917 CGCG CG^CG
AhdI 6024 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 6024 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1824 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4577 TGCGCA TGC^GCA
AvrII 1599 CCTAGG C^CTAGG
BamHI 7241 GGATCC G^GATCC
BbrPI 439 CACGTG CAC^GTG
BcgI 3924 GCAGCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5670 TGATCA T^GATCA
BlnI 1599 CCTAGG C^CTAGG
BsiWI 6192 CGTACG C^GTACG
Bsp1407I 7052 TGTACA T^GTACA
BsrGI 7052 TGTACA T^GTACA
BstUI 6917 CGCG CG^CG
CpoI 4805 CGGACCG CG^G[AT]CCG
DraIII 1904 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 6024 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3199 ATGCAT ATGCA^T
FbaI 5670 TGATCA T^GATCA
FspI 4577 TGCGCA TGC^GCA
HindIII 294 AAGCTT A^AGCTT
MseI 1925 TTAA T^TAA
MvnI 6917 CGCG CG^CG
NaeI 3370 GCCGGC GCC^GGC
NdeI 6613 CATATG CA^TATG
NgoMIV 3370 GCCGGC G^CCGGC
NheI 6601 GCTAGC G^CTAGC
NotI 2292 GCGGCCGC GC^GGCCGC
NsiI 3199 ATGCAT ATGCA^T
PflFI 1824 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 439 CACGTG CAC^GTG
PmlI 439 CACGTG CAC^GTG
PshAI 2355 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4805 CGGACCG CG^G[AT]CCG
SacI 3742 GAGCTC GAGCT^C
SapI 2588 GCTCTTC GCTCTTC(1/4)
SbfI 5974 CCTGCAGG CCTGCA^GG
ScaI 4199 AGTACT AGT^ACT
SgrAI 3997 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 5974 CCTGCAGG CCTGCA^GG
SspBI 7052 TGTACA T^GTACA
Tru9I 1925 TTAA T^TAA
Tth111I 1824 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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Asp700I 70 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3801 GAACCGCTTC
BsaAI 439 CACGTG [CT]AC^GT[GA]
5812 TACGTG
BsaWI 4364 ACCGGA [AT]^CCGG[AT]
6373 ACCGGA
BspLU11I 518 ACATGT A^CATGT
3940 ACATGT
EagI 416 CGGCCG C^GGCCG
2293 CGGCCG
EclXI 416 CGGCCG C^GGCCG
2293 CGGCCG
Eco52I 416 CGGCCG C^GGCCG
2293 CGGCCG
EcoRI 1102 GAATTC G^AATTC
5167 GAATTC
EcoRV 1475 GATATC GAT^ATC
2067 GATATC
HincII 1263 GTCGAC GT[CT]^[GA]AC
6628 GTCGAC
HindII 1263 GTCGAC GT[CT]^[GA]AC
6628 GTCGAC
PaeR7I 302 CTCGAG C^TCGAG
4259 CTCGAG
SalI 1263 GTCGAC G^TCGAC
6628 GTCGAC
SmaI 2520 CCCGGG CCC^GGG
3863 CCCGGG
XhoI 302 CTCGAG C^TCGAG
4259 CTCGAG
XmaCI 2520 CCCGGG C^CCGGG
3863 CCCGGG
XmaI 2520 CCCGGG C^CCGGG
3863 CCCGGG
XmnI 70 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3801 GAACCGCTTC
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# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII