Restriction map for
pFN21AA0708
(Linear) (Six-base) MAPSORT of: KIAA0708.seq Check: 5712 from: 1 to: 7551
KIAA0708.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 6349 6547 7551
Size: 6349 198 1004
Fragments arranged by size:
6349 1004 198
AccI GT'mk_AC
Cuts at: 0 215 1264 1747 6629 7551
Size: 215 1049 483 4882 922
Fragments arranged by size:
4882 1049 922 483 215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 154 250 468 728 1325 2413 4323
Size: 86 68 96 218 260 597 1088 1910
Cuts at: 4323 4705 5401 7285 7551
Size: 382 696 1884 266
Fragments arranged by size:
1910 1884 1088 696 597 382 266 260
218 96 86 68
AfeI AGC'GCT
Cuts at: 0 784 1096 3838 6703 7551
Size: 784 312 2742 2865 848
Fragments arranged by size:
2865 2742 848 784 312
AflIII A'CryG_T
Cuts at: 0 518 2684 3940 7551
Size: 518 2166 1256 3611
Fragments arranged by size:
3611 2166 1256 518
AhdI GACnn_n'nnGTC
Cuts at: 0 6029 7551
Size: 6029 1522
AleI CACnn'nnGTG
Cuts at: 0 3632 4634 5486 7551
Size: 3632 1002 852 2065
Fragments arranged by size:
3632 2065 1002 852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 583 757 4073 7145 7246 7292 7551
Size: 583 174 3316 3072 101 46 259
Fragments arranged by size:
3316 3072 583 259 174 101 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 7551
Size: 886 32 6633
Fragments arranged by size:
6633 886 32
AlwNI CAG_nnn'CTG
Cuts at: 0 363 576 1348 1935 2253 3168 3285 4098
Size: 363 213 772 587 318 915 117 813
Cuts at: 4098 4908 5304 5844 5944 6114 6522 7014 7551
Size: 810 396 540 100 170 408 492 537
Fragments arranged by size:
915 813 810 772 587 540 537 492
408 396 363 318 213 170 117 100
ApaI G_GGCC'C
Cuts at: 0 470 960 1315 2875 3020 3892 4482 5516
Size: 470 490 355 1560 145 872 590 1034
Cuts at: 5516 5876 7551
Size: 360 1675
Fragments arranged by size:
1675 1560 1034 872 590 490 470 360
355 145
ApaLI G'TGCA_C
Cuts at: 0 1285 6477 6897 7551
Size: 1285 5192 420 654
Fragments arranged by size:
5192 1285 654 420
ApoI r'AATT_y
Cuts at: 0 1102 4053 5167 5341 7551
Size: 1102 2951 1114 174 2210
Fragments arranged by size:
2951 2210 1114 1102 174
AvaI C'yCGr_G
Cuts at: 0 302 2520 3863 4259 4553 7420 7551
Size: 302 2218 1343 396 294 2867 131
Fragments arranged by size:
2867 2218 1343 396 302 294 131
AvrII C'CTAG_G
Cuts at: 0 1599 7551
Size: 1599 5952
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1752 1785 7551
Size: 1752 33 5766
Fragments arranged by size:
5766 1752 33
BamHI G'GATC_C
Cuts at: 0 7241 7551
Size: 7241 310
BanI G'GyrC_C
Cuts at: 0 33 2935 4001 4374 4594 4727 6743 7551
Size: 33 2902 1066 373 220 133 2016 808
Fragments arranged by size:
2902 2016 1066 808 373 220 133 33
BanII G_rGCy'C
Cuts at: 0 470 501 606 960 1315 1874 2105 2527
Size: 470 31 105 354 355 559 231 422
Cuts at: 2527 2875 3020 3116 3478 3746 3892 4372 4482
Size: 348 145 96 362 268 146 480 110
Cuts at: 4482 4695 5516 5867 5876 5995 6226 7551
Size: 213 821 351 9 119 231 1325
Fragments arranged by size:
1325 821 559 480 470 422 362 355
354 351 348 268 231 231 213 146
145 119 110 105 96 31 9
BbeI G_GCGC'C
Cuts at: 0 4005 4378 4598 7551
Size: 4005 373 220 2953
Fragments arranged by size:
4005 2953 373 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 474 1077 3410 5614 7291 7406 7551
Size: 474 603 2333 2204 1677 115 145
Fragments arranged by size:
2333 2204 1677 603 474 145 115
BbvCI CC'TCA_GC
Cuts at: 0 967 3046 7213 7551
Size: 967 2079 4167 338
Fragments arranged by size:
4167 2079 967 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3913 3947 7551
Size: 3913 34 3604
Fragments arranged by size:
3913 3604 34
BclI T'GATC_A
Cuts at: 0 5670 7551
Size: 5670 1881
BfrBI ATG'CAT
Cuts at: 0 3201 7551
Size: 3201 4350
BglI GCCn_nnn'nGGC
Cuts at: 0 42 1118 2921 3134 3848 4093 5871 6518
Size: 42 1076 1803 213 714 245 1778 647
Cuts at: 6518 7327 7425 7551
Size: 809 98 126
Fragments arranged by size:
1803 1778 1076 809 714 647 245 213
126 98 42
BglII A'GATC_T
Cuts at: 0 1607 1700 2489 7551
Size: 1607 93 789 5062
Fragments arranged by size:
5062 1607 789 93
BlpI GC'TnA_GC
Cuts at: 0 3278 4429 5279 6071 7551
Size: 3278 1151 850 792 1480
Fragments arranged by size:
3278 1480 1151 850 792
Bme1580I G_kGCm'C
Cuts at: 0 38 252 470 960 1289 1315 1384 2875
Size: 38 214 218 490 329 26 69 1491
Cuts at: 2875 3020 3892 3966 4179 4482 5429 5516 5876
Size: 145 872 74 213 303 947 87 360
Cuts at: 5876 6481 6901 6971 7421 7551
Size: 605 420 70 450 130
Fragments arranged by size:
1491 947 872 605 490 450 420 360
329 303 218 214 213 145 130 87
74 70 69 38 26
BmgBI CAC'GTC
Cuts at: 0 609 800 5264 7551
Size: 609 191 4464 2287
Fragments arranged by size:
4464 2287 609 191
BmrI ACTGGGnnnn_n'
Cuts at: 0 1290 3588 4108 4413 6105 7551
Size: 1290 2298 520 305 1692 1446
Fragments arranged by size:
2298 1692 1446 1290 520 305
BmtI G_CTAG'C
Cuts at: 0 6605 7551
Size: 6605 946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 620 1931 2604 3267 3543 6432 6477 7551
Size: 620 1311 673 663 276 2889 45 1074
Fragments arranged by size:
2889 1311 1074 673 663 620 276 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 863 919 1092 1132 1321 1426 2947
Size: 700 163 56 173 40 189 105 1521
Cuts at: 2947 3342 3462 3993 3994 4718 4858 4939 5178
Size: 395 120 531 1 724 140 81 239
Cuts at: 5178 5398 5713 5843 7036 7135 7168 7551
Size: 220 315 130 1193 99 33 383
Fragments arranged by size:
1521 1193 724 700 531 395 383 315
239 220 189 173 163 140 130 120
105 99 81 56 40 33 1
Bpu10I CC'TnA_GC
Cuts at: 0 533 967 2792 2843 3046 3893 4319 4816
Size: 533 434 1825 51 203 847 426 497
Cuts at: 4816 4909 6137 6179 7213 7551
Size: 93 1228 42 1034 338
Fragments arranged by size:
1825 1228 1034 847 533 497 434 426
338 203 93 51 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2938 3663 4254 5634 6781 7551
Size: 2938 725 591 1380 1147 770
Fragments arranged by size:
2938 1380 1147 770 725 591
BsaI GGTCTCn'nnnn_
Cuts at: 0 839 1573 1885 2648 2803 2965 4210 5365
Size: 839 734 312 763 155 162 1245 1155
Cuts at: 5365 7166 7551
Size: 1801 385
Fragments arranged by size:
1801 1245 1155 839 763 734 385 312
162 155
BsaAI yAC'GTr
Cuts at: 0 441 5814 7551
Size: 441 5373 1737
Fragments arranged by size:
5373 1737 441
BsaHI Gr'CG_yC
Cuts at: 0 4002 4375 4595 7551
Size: 4002 373 220 2956
Fragments arranged by size:
4002 2956 373 220
BsaWI w'CCGG_w
Cuts at: 0 4364 6373 7551
Size: 4364 2009 1178
Fragments arranged by size:
4364 2009 1178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 1118 1148 1780 1810 2924 2954
Size: 838 30 250 30 632 30 1114 30
Cuts at: 2954 3340 3370 3487 3517 3913 3943 5176 5206
Size: 386 30 117 30 396 30 1233 30
Cuts at: 5206 5521 5551 6859 6889 7165 7195 7453 7483
Size: 315 30 1308 30 276 30 258 30
Cuts at: 7483 7551
Size: 68
Fragments arranged by size:
1308 1233 1114 838 632 396 386 315
276 258 250 117 68 30 30 30
30 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1201 1801 2143 2406 2530 2941 3997 4292
Size: 1201 600 342 263 124 411 1056 295
Cuts at: 4292 4936 5008 5032 5035 5044 5371 6390 7173
Size: 644 72 24 3 9 327 1019 783
Cuts at: 7173 7359 7405 7408 7462 7551
Size: 186 46 3 54 89
Fragments arranged by size:
1201 1056 1019 783 644 600 411 342
327 295 263 186 124 89 72 54
46 24 9 3 3
BseYI C'CCAG_C
Cuts at: 0 19 280 592 834 953 1033 1198 2377
Size: 19 261 312 242 119 80 165 1179
Cuts at: 2377 2975 3288 3390 3506 4112 4710 5268 5516
Size: 598 313 102 116 606 598 558 248
Cuts at: 5516 5746 5932 6157 6365 6554 6709 7551
Size: 230 186 225 208 189 155 842
Fragments arranged by size:
1179 842 606 598 598 558 313 312
261 248 242 230 225 208 189 186
165 155 119 116 102 80 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1156 1267 2083 2266 2389 2819 3604
Size: 575 581 111 816 183 123 430 785
Cuts at: 3604 4159 4459 5341 5776 6058 6733 7105 7188
Size: 555 300 882 435 282 675 372 83
Cuts at: 7188 7551
Size: 363
Fragments arranged by size:
882 816 785 675 581 575 555 435
430 372 363 300 282 183 123 111
83
BsiEI CG_ry'CG
Cuts at: 0 419 2051 2296 6501 7551
Size: 419 1632 245 4205 1050
Fragments arranged by size:
4205 1632 1050 419 245
BsiHKAI G_wGCw'C
Cuts at: 0 185 446 1289 2590 3224 3746 4520 4784
Size: 185 261 843 1301 634 522 774 264
Cuts at: 4784 4915 6481 6901 7551
Size: 131 1566 420 650
Fragments arranged by size:
1566 1301 843 774 650 634 522 420
264 261 185 131
BsiWI C'GTAC_G
Cuts at: 0 6192 7551
Size: 6192 1359
BsmI GAATG_Cn'
Cuts at: 0 734 1235 3377 7272 7551
Size: 734 501 2142 3895 279
Fragments arranged by size:
3895 2142 734 501 279
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4816 5124 6037 6402 7551
Size: 4816 308 913 365 1149
Fragments arranged by size:
4816 1149 913 365 308
Bsp1286I G_dGCh'C
Cuts at: 0 38 185 252 446 470 501 606 960
Size: 38 147 67 194 24 31 105 354
Cuts at: 960 1289 1315 1384 1874 2105 2527 2590 2875
Size: 329 26 69 490 231 422 63 285
Cuts at: 2875 3020 3116 3224 3478 3746 3892 3966 4179
Size: 145 96 108 254 268 146 74 213
Cuts at: 4179 4372 4482 4520 4695 4784 4915 5429 5516
Size: 193 110 38 175 89 131 514 87
Cuts at: 5516 5867 5876 5995 6226 6481 6901 6971 7421
Size: 351 9 119 231 255 420 70 450
Cuts at: 7421 7551
Size: 130
Fragments arranged by size:
514 490 450 422 420 354 351 329
285 268 255 254 231 231 213 194
193 175 147 146 145 131 130 119
110 108 105 96 89 87 74 70
69 67 63 38 38 31 26 24
9
BspHI T'CATG_A
Cuts at: 0 2273 2750 5502 7551
Size: 2273 477 2752 2049
Fragments arranged by size:
2752 2273 2049 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 1239 1635 1694 2096 2276 3181 4117
Size: 515 724 396 59 402 180 905 936
Cuts at: 4117 4437 6254 6349 6547 7551
Size: 320 1817 95 198 1004
Fragments arranged by size:
1817 1004 936 905 724 515 402 396
320 198 180 95 59
BsrBI CCG'CTC
Cuts at: 0 246 2142 2298 5857 7551
Size: 246 1896 156 3559 1694
Fragments arranged by size:
3559 1896 1694 246 156
BsrDI GCAATG_nn'
Cuts at: 0 338 673 3089 6347 7551
Size: 338 335 2416 3258 1204
Fragments arranged by size:
3258 2416 1204 338 335
BsrFI r'CCGG_y
Cuts at: 0 3370 3598 3998 5831 7551
Size: 3370 228 400 1833 1720
Fragments arranged by size:
3370 1833 1720 400 228
BsrGI T'GTAC_A
Cuts at: 0 7052 7551
Size: 7052 499
BssSI C'ACGA_G
Cuts at: 0 2284 3100 4045 5422 7551
Size: 2284 816 945 1377 2129
Fragments arranged by size:
2284 2129 1377 945 816
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2253 3728 4109 5304 6027 6509 7551
Size: 2253 1475 381 1195 723 482 1042
Fragments arranged by size:
2253 1475 1195 1042 723 482 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 592 634 1989 2223 3261 3366 3702
Size: 387 205 42 1355 234 1038 105 336
Cuts at: 3702 5111 5524 7551
Size: 1409 413 2027
Fragments arranged by size:
2027 1409 1355 1038 413 387 336 234
205 105 42
BstYI r'GATC_y
Cuts at: 0 306 542 749 1304 1607 1700 1776 2423
Size: 306 236 207 555 303 93 76 647
Cuts at: 2423 2489 3245 3824 4667 5067 5840 6979 7139
Size: 66 756 579 843 400 773 1139 160
Cuts at: 7139 7241 7551
Size: 102 310
Fragments arranged by size:
1139 843 773 756 647 579 555 400
310 306 303 236 207 160 102 93
76 66
Bsu36I CC'TnA_GG
Cuts at: 0 39 209 1316 3077 3457 4405 5436 6584
Size: 39 170 1107 1761 380 948 1031 1148
Cuts at: 6584 7371 7551
Size: 787 180
Fragments arranged by size:
1761 1148 1107 1031 948 787 380 180
170 39
BtgI C'CryG_G
Cuts at: 0 1619 1898 3630 5646 5867 6774 6842 7551
Size: 1619 279 1732 2016 221 907 68 709
Fragments arranged by size:
2016 1732 1619 907 709 279 221 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3169 7551
Size: 3169 4382
BtsI GCAGTG_nn'
Cuts at: 0 714 3150 4587 4619 5263 5529 6142 6204
Size: 714 2436 1437 32 644 266 613 62
Cuts at: 6204 7551
Size: 1347
Fragments arranged by size:
2436 1437 1347 714 644 613 266 62
32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4758 4793 7551
Size: 4758 35 2758
Fragments arranged by size:
4758 2758 35
DraIII CAC_nnn'GTG
Cuts at: 0 1909 7551
Size: 1909 5642
EaeI y'GGCC_r
Cuts at: 0 377 416 994 1628 1991 2054 2293 2402
Size: 377 39 578 634 363 63 239 109
Cuts at: 2402 2442 3368 3665 3907 4766 6609 6683 7551
Size: 40 926 297 242 859 1843 74 868
Fragments arranged by size:
1843 926 868 859 634 578 377 363
297 242 239 109 74 63 40 39
EagI C'GGCC_G
Cuts at: 0 416 2293 7551
Size: 416 1877 5258
Fragments arranged by size:
5258 1877 416
EarI CTCTTCn'nnn_
Cuts at: 0 1778 1814 2595 3952 4951 5009 5021 5030
Size: 1778 36 781 1357 999 58 12 9
Cuts at: 5030 5596 7382 7520 7551
Size: 566 1786 138 31
Fragments arranged by size:
1786 1778 1357 999 781 566 138 58
36 31 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 535 1078 4819 7254 7551
Size: 535 543 3741 2435 297
Fragments arranged by size:
3741 2435 543 535 297
EcoICRI GAG'CTC
Cuts at: 0 3744 7551
Size: 3744 3807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 154 250 468 700 728 863 919
Size: 86 68 96 218 232 28 135 56
Cuts at: 919 1092 1132 1321 1325 1426 2413 2947 3342
Size: 173 40 189 4 101 987 534 395
Cuts at: 3342 3462 3993 3994 4323 4705 4718 4858 4939
Size: 120 531 1 329 382 13 140 81
Cuts at: 4939 5178 5398 5401 5713 5843 7036 7135 7168
Size: 239 220 3 312 130 1193 99 33
Cuts at: 7168 7285 7551
Size: 117 266
Fragments arranged by size:
1193 987 534 531 395 382 329 312
266 239 232 220 218 189 173 140
135 130 120 117 101 99 96 86
81 68 56 40 33 28 13 4
3 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2116 2965 5461 7147 7551
Size: 2116 849 2496 1686 404
Fragments arranged by size:
2496 2116 1686 849 404
EcoO109I rG'GnC_Cy
Cuts at: 0 24 43 401 467 1030 1312 1596 1851
Size: 24 19 358 66 563 282 284 255
Cuts at: 1851 2008 2250 2837 2871 2945 3016 3306 3882
Size: 157 242 587 34 74 71 290 576
Cuts at: 3882 3889 4119 4478 4479 5513 5532 5658 5872
Size: 7 230 359 1 1034 19 126 214
Cuts at: 5872 5873 6132 6519 7336 7551
Size: 1 259 387 817 215
Fragments arranged by size:
1034 817 587 576 563 387 359 358
290 284 282 259 255 242 230 215
214 157 126 74 71 66 34 24
19 19 7 1 1
EcoRI G'AATT_C
Cuts at: 0 1102 5167 7551
Size: 1102 4065 2384
Fragments arranged by size:
4065 2384 1102
EcoRV GAT'ATC
Cuts at: 0 1477 2069 7551
Size: 1477 592 5482
Fragments arranged by size:
5482 1477 592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1545 4992 7551
Size: 1545 3447 2559
Fragments arranged by size:
3447 2559 1545
FspI TGC'GCA
Cuts at: 0 4579 7551
Size: 4579 2972
HaeII r_GCGC'y
Cuts at: 0 364 786 1098 2961 3840 4005 4378 4598
Size: 364 422 312 1863 879 165 373 220
Cuts at: 4598 4935 6104 6436 6705 6792 7551
Size: 337 1169 332 269 87 759
Fragments arranged by size:
1863 1169 879 759 422 373 364 337
332 312 269 220 165 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 838 870 1780 1812 1813 1845
Size: 113 32 693 32 910 32 1 32
Cuts at: 1845 2438 2470 3256 3288 5176 5208 5347 5379
Size: 593 32 786 32 1888 32 139 32
Cuts at: 5379 5764 5796 6015 6047 6235 6267 7165 7197
Size: 385 32 219 32 188 32 898 32
Cuts at: 7197 7453 7485 7551
Size: 256 32 66
Fragments arranged by size:
1888 910 898 786 693 593 385 256
219 188 139 113 66 32 32 32
32 32 32 32 32 32 32 32
32 32 1
HincII GTy'rAC
Cuts at: 0 1265 6630 7551
Size: 1265 5365 921
Fragments arranged by size:
5365 1265 921
HindIII A'AGCT_T
Cuts at: 0 294 7551
Size: 294 7257
KasI G'GCGC_C
Cuts at: 0 4001 4374 4594 7551
Size: 4001 373 220 2957
Fragments arranged by size:
4001 2957 373 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1505 1778 3554 3611 6488 7551
Size: 1505 273 1776 57 2877 1063
Fragments arranged by size:
2877 1776 1505 1063 273 57
MscI TGG'CCA
Cuts at: 0 1993 2056 3667 3909 4768 6611 6685 7551
Size: 1993 63 1611 242 859 1843 74 866
Fragments arranged by size:
1993 1843 1611 866 859 242 74 63
MslI CAynn'nnrTG
Cuts at: 0 190 439 517 913 1966 2749 3382 3632
Size: 190 249 78 396 1053 783 633 250
Cuts at: 3632 4634 5486 6658 7087 7127 7222 7551
Size: 1002 852 1172 429 40 95 329
Fragments arranged by size:
1172 1053 1002 852 783 633 429 396
329 250 249 190 95 78 40
MspA1I CmG'CkG
Cuts at: 0 280 387 421 816 891 1348 1920 2047
Size: 280 107 34 395 75 457 572 127
Cuts at: 2047 3282 3728 3927 4725 4893 4959 5676 5970
Size: 1235 446 199 798 168 66 717 294
Cuts at: 5970 6111 6157 6713 7551
Size: 141 46 556 838
Fragments arranged by size:
1235 838 798 717 572 556 457 446
395 294 280 199 168 141 127 107
75 66 46 34
NaeI GCC'GGC
Cuts at: 0 3372 7551
Size: 3372 4179
NarI GG'CG_CC
Cuts at: 0 4002 4375 4595 7551
Size: 4002 373 220 2956
Fragments arranged by size:
4002 2956 373 220
NcoI C'CATG_G
Cuts at: 0 1898 5646 5867 6774 6842 7551
Size: 1898 3748 221 907 68 709
Fragments arranged by size:
3748 1898 907 709 221 68
NdeI CA'TA_TG
Cuts at: 0 6614 7551
Size: 6614 937
NgoMIV G'CCGG_C
Cuts at: 0 3370 7551
Size: 3370 4181
NheI G'CTAG_C
Cuts at: 0 6601 7551
Size: 6601 950
NotI GC'GGCC_GC
Cuts at: 0 2293 7551
Size: 2293 5258
NsiI A_TGCA'T
Cuts at: 0 3203 7551
Size: 3203 4348
NspI r_CATG'y
Cuts at: 0 19 522 984 1299 2041 2343 3627 3690
Size: 19 503 462 315 742 302 1284 63
Cuts at: 3690 3944 4294 5311 6267 7347 7551
Size: 254 350 1017 956 1080 204
Fragments arranged by size:
1284 1080 1017 956 742 503 462 350
315 302 254 204 63 19
PasI CC'CwG_GG
Cuts at: 0 229 1584 4179 5730 6228 6489 7551
Size: 229 1355 2595 1551 498 261 1062
Fragments arranged by size:
2595 1551 1355 1062 498 261 229
PciI A'CATG_T
Cuts at: 0 518 3940 7551
Size: 518 3422 3611
Fragments arranged by size:
3611 3422 518
PflMI CCAn_nnn'nTGG
Cuts at: 0 1935 2154 2649 3905 4098 4284 6522 7014
Size: 1935 219 495 1256 193 186 2238 492
Cuts at: 7014 7551
Size: 537
Fragments arranged by size:
2238 1935 1256 537 495 492 219 193
186
PfoI T'CCnGG_A
Cuts at: 0 309 806 2891 3260 3917 5228 5721 6802
Size: 309 497 2085 369 657 1311 493 1081
Cuts at: 6802 7145 7244 7551
Size: 343 99 307
Fragments arranged by size:
2085 1311 1081 657 497 493 369 343
309 307 99
PmlI CAC'GTG
Cuts at: 0 441 7551
Size: 441 7110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 4135 4167 4809 4841 7551
Size: 1198 32 2905 32 642 32 2710
Fragments arranged by size:
2905 2710 1198 642 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 24 401 2008 2837 3306 3882 6132 7336
Size: 24 377 1607 829 469 576 2250 1204
Cuts at: 7336 7551
Size: 215
Fragments arranged by size:
2250 1607 1204 829 576 469 377 215
24
PshAI GACnn'nnGTC
Cuts at: 0 2359 7551
Size: 2359 5192
PsiI TTA'TAA
Cuts at: 0 2665 7551
Size: 2665 4886
PspOMI G'GGCC_C
Cuts at: 0 466 956 1311 2871 3016 3888 4478 5512
Size: 466 490 355 1560 145 872 590 1034
Cuts at: 5512 5872 7551
Size: 360 1679
Fragments arranged by size:
1679 1560 1034 872 590 490 466 360
355 145
PstI C_TGCA'G
Cuts at: 0 56 572 1646 2093 2556 2598 2883 4470
Size: 56 516 1074 447 463 42 285 1587
Cuts at: 4470 4607 4904 4931 5258 5288 5979 6119 7139
Size: 137 297 27 327 30 691 140 1020
Cuts at: 7139 7271 7551
Size: 132 280
Fragments arranged by size:
1587 1074 1020 691 516 463 447 327
297 285 280 140 137 132 56 42
30 27
PvuII CAG'CTG
Cuts at: 0 280 387 816 1348 1920 3282 3728 3927
Size: 280 107 429 532 572 1362 446 199
Cuts at: 3927 4893 5676 5970 6111 6157 6713 7551
Size: 966 783 294 141 46 556 838
Fragments arranged by size:
1362 966 838 783 572 556 532 446
429 294 280 199 141 107 46
RsrII CG'GwC_CG
Cuts at: 0 4806 7551
Size: 4806 2745
SacI G_AGCT'C
Cuts at: 0 3746 7551
Size: 3746 3805
SalI G'TCGA_C
Cuts at: 0 1263 6628 7551
Size: 1263 5365 923
Fragments arranged by size:
5365 1263 923
SanDI GG'GwC_CC
Cuts at: 0 401 2008 2837 7551
Size: 401 1607 829 4714
Fragments arranged by size:
4714 1607 829 401
SapI GCTCTTCn'nnn_
Cuts at: 0 2595 7551
Size: 2595 4956
SbfI CC_TGCA'GG
Cuts at: 0 5979 7551
Size: 5979 1572
ScaI AGT'ACT
Cuts at: 0 4201 7551
Size: 4201 3350
SexAI A'CCwGG_T
Cuts at: 0 2219 3308 6472 7551
Size: 2219 1089 3164 1079
Fragments arranged by size:
3164 2219 1089 1079
SfcI C'TryA_G
Cuts at: 0 52 568 1642 2089 2552 2594 2879 3342
Size: 52 516 1074 447 463 42 285 463
Cuts at: 3342 4466 4603 4900 4927 5202 5254 5284 5965
Size: 1124 137 297 27 275 52 30 681
Cuts at: 5965 5975 6115 6123 6573 7062 7135 7267 7551
Size: 10 140 8 450 489 73 132 284
Fragments arranged by size:
1124 1074 681 516 489 463 463 450
447 297 285 284 275 140 137 132
73 52 52 42 30 27 10 8
SfoI GGC'GCC
Cuts at: 0 4003 4376 4596 7551
Size: 4003 373 220 2955
Fragments arranged by size:
4003 2955 373 220
SgrAI Cr'CCGG_yG
Cuts at: 0 3998 7551
Size: 3998 3553
SmaI CCC'GGG
Cuts at: 0 2522 3865 7551
Size: 2522 1343 3686
Fragments arranged by size:
3686 2522 1343
SmlI C'TyrA_G
Cuts at: 0 302 2953 3678 4233 4259 5613 6796 7551
Size: 302 2651 725 555 26 1354 1183 755
Fragments arranged by size:
2651 1354 1183 755 725 555 302 26
SphI G_CATG'C
Cuts at: 0 19 2343 6267 7551
Size: 19 2324 3924 1284
Fragments arranged by size:
3924 2324 1284 19
SrfI GCCC'GGGC
Cuts at: 0 2522 7551
Size: 2522 5029
StuI AGG'CCT
Cuts at: 0 87 317 5698 7321 7366 7543 7551
Size: 87 230 5381 1623 45 177 8
Fragments arranged by size:
5381 1623 230 177 87 45 8
StyI C'CwwG_G
Cuts at: 0 773 1599 1898 5646 5867 6774 6842 7551
Size: 773 826 299 3748 221 907 68 709
Fragments arranged by size:
3748 907 826 773 709 299 221 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1272 1576 1903 7551
Size: 148 1124 304 327 5648
Fragments arranged by size:
5648 1124 327 304 148
TatI w'GTAC_w
Cuts at: 0 4199 5767 7052 7079 7551
Size: 4199 1568 1285 27 472
Fragments arranged by size:
4199 1568 1285 472 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1374 1406 2729 2761 2924 2956
Size: 1118 32 224 32 1323 32 163 32
Cuts at: 2956 3487 3519 3913 3945 5521 5553 7551
Size: 531 32 394 32 1576 32 1998
Fragments arranged by size:
1998 1576 1323 1118 531 394 224 163
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1827 7551
Size: 1827 5724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2830 5088 5743 7551
Size: 2830 2258 655 1808
Fragments arranged by size:
2830 2258 1808 655
XhoI C'TCGA_G
Cuts at: 0 302 4259 7551
Size: 302 3957 3292
Fragments arranged by size:
3957 3292 302
XmaI C'CCGG_G
Cuts at: 0 2520 3863 7551
Size: 2520 1343 3688
Fragments arranged by size:
3688 2520 1343
XmnI GAAnn'nnTTC
Cuts at: 0 74 3805 7551
Size: 74 3731 3746
Fragments arranged by size:
3746 3731 74
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AscI AseI AsiSI
BciVI BsaBI BspEI BssHII BstBI BstEII BstZ17I ClaI
DraI DrdI FseI FspAI HpaI KpnI MfeI MluI
NruI PacI PmeI PspXI PsrI PvuI SacII SfiI
SgfI SnaBI SpeI SspI SwaI XbaI ZraI