(Circular) (Six-base) MAPSORT of: pFN21AE1013.seq Check: 1064 from: 1 to: 5560 pFN21AE1013 5560 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2289 2903 1937 Size: 352 614 4594 Fragments arranged by size: 4594 614 352 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3623 5007 5275 1632 Size: 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 2367 4459 5436 1203 Size: 1164 2092 977 1327 Fragments arranged by size: 2092 1327 1164 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 2426 4289 2426 Size: 1863 3697 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 3303 4875 3303 Size: 1572 3988 ApaLI G'TGCA_C Cuts at: 2354 3617 4773 2354 Size: 1263 1156 3141 Fragments arranged by size: 3141 1263 1156 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2885 1870 Size: 73 249 693 4545 Fragments arranged by size: 4545 693 249 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2881 4336 618 Size: 325 217 627 1094 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2655 2879 1391 Size: 1264 224 4072 Fragments arranged by size: 4072 1264 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BbvCI CC'TCA_GC Cuts at: 2618 2618 Size: 5560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2533 3452 4662 2533 Size: 919 1210 3431 Fragments arranged by size: 3431 1210 919 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 2056 2358 2502 2637 3621 4777 1790 Size: 266 302 144 135 984 1156 2573 Fragments arranged by size: 2573 1156 984 302 266 144 135 BmgBI CAC'GTC Cuts at: 1318 2683 1318 Size: 1365 4195 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 2771 2843 4220 1239 Size: 1024 508 72 1377 2579 Fragments arranged by size: 2579 1377 1024 508 72 Bpu10I CC'TnA_GC Cuts at: 2618 2618 Size: 5560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 2727 3367 3749 Size: 53 83 186 936 1062 132 640 382 Cuts at: 3749 4384 275 Size: 635 1451 Fragments arranged by size: 1451 1062 936 640 635 382 186 132 83 53 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5335 Fragments arranged by size: 5335 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2031 2034 2054 2057 2225 1186 Size: 845 3 20 3 168 4521 Fragments arranged by size: 4521 845 168 20 3 3 BseYI C'CCAG_C Cuts at: 4763 5259 4763 Size: 496 5064 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2029 2813 1163 Size: 573 293 784 3910 Fragments arranged by size: 3910 784 573 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2358 2610 2655 3621 3706 4777 2193 Size: 165 252 45 966 85 1071 2976 Fragments arranged by size: 2976 1071 966 252 165 85 45 BsmI GAATG_Cn' Cuts at: 2316 3071 3164 2316 Size: 755 93 4712 Fragments arranged by size: 4712 755 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2747 2747 Size: 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2193 2358 2502 2610 2637 2655 Size: 399 266 137 165 144 108 27 18 Cuts at: 2655 2879 3621 3706 4777 1391 Size: 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 266 224 165 144 137 108 85 27 18 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 2490 3449 5179 2490 Size: 959 1730 2871 Fragments arranged by size: 2871 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2915 844 Size: 2071 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2788 3447 1394 Size: 429 965 659 3507 Fragments arranged by size: 3507 965 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2557 4204 4391 1700 Size: 110 747 1647 187 2869 Fragments arranged by size: 2869 1647 747 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555 Size: 918 367 385 17 1452 86 12 346 Cuts at: 5555 1972 Size: 1977 Fragments arranged by size: 1977 1452 918 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5008 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 2222 3076 3870 3890 2222 Size: 854 794 20 3892 Fragments arranged by size: 3892 854 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2587 2718 3007 Size: 54 1425 50 275 18 757 131 289 Cuts at: 3007 3896 5525 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 757 289 275 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 2760 3490 1353 Size: 1169 238 730 3423 Fragments arranged by size: 3423 1169 730 238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 2653 Size: 5560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2263 2771 2843 3623 4220 5007 5275 Size: 393 631 508 72 780 597 787 268 Cuts at: 5275 1239 Size: 1524 Fragments arranged by size: 1524 787 780 631 597 508 393 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 2519 2519 Size: 5560 EcoO109I rG'GnC_Cy Cuts at: 2065 2141 2251 2776 2065 Size: 76 110 525 4849 Fragments arranged by size: 4849 525 110 76 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 2032 4066 2032 Size: 2034 3526 HaeII r_GCGC'y Cuts at: 1678 4707 1678 Size: 3029 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2412 2444 4201 Size: 32 135 32 689 32 119 32 1757 Cuts at: 4201 4233 4275 4307 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1757 689 135 119 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4674 4858 1305 Size: 3369 184 2007 Fragments arranged by size: 3369 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3738 Fragments arranged by size: 3738 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3518 3877 4036 518 Size: 648 1329 1023 359 159 2042 Fragments arranged by size: 2042 1329 1023 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 2976 3653 4801 5046 1606 Size: 786 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 584 245 NaeI GCC'GGC Cuts at: 2559 2559 Size: 5560 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NgoMIV G'CCGG_C Cuts at: 2557 2557 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2833 2918 4463 1132 Size: 97 1604 85 1545 2229 Fragments arranged by size: 2229 1604 1545 97 85 PasI CC'CwG_GG Cuts at: 1348 2056 1348 Size: 708 4852 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PfoI T'CCnGG_A Cuts at: 2520 2676 2520 Size: 156 5404 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2592 2624 3629 3661 5142 5174 2592 Size: 32 1005 32 1481 32 2978 Fragments arranged by size: 2978 1481 1005 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2251 2141 Size: 110 5450 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2229 2912 830 Size: 1399 683 3478 Fragments arranged by size: 3478 1399 683 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SacI G_AGCT'C Cuts at: 2655 2655 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SanDI GG'GwC_CC Cuts at: 2141 2141 Size: 5560 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SfcI C'TryA_G Cuts at: 826 1045 2225 2908 4043 4724 4915 826 Size: 219 1180 683 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 683 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2999 513 Size: 552 1934 3074 Fragments arranged by size: 3074 1934 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 5518 96 Size: 274 80 33 51 1443 1829 1712 138 Fragments arranged by size: 1829 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 2419 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 2204 2369 3689 2204 Size: 165 1320 4075 Fragments arranged by size: 4075 1320 165 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI