Restriction map for
pFN21AE1013
(Linear) (Six-base) MAPSORT of: KIEE1013.seq Check: 470 from: 1 to: 855
KIEE1013.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 0 284 855
Size: 284 571
AflIII A'CryG_T
Cuts at: 0 362 855
Size: 362 493
AhdI GACnn_n'nnGTC
Cuts at: 0 421 855
Size: 421 434
ApaLI G'TGCA_C
Cuts at: 0 349 855
Size: 349 506
AvaI C'yCGr_G
Cuts at: 0 187 855
Size: 187 668
BanII G_rGCy'C
Cuts at: 0 650 855
Size: 650 205
BbvCI CC'TCA_GC
Cuts at: 0 613 855
Size: 613 242
BciVI GTATCCnnnnn_n'
Cuts at: 0 528 855
Size: 528 327
Bme1580I G_kGCm'C
Cuts at: 0 51 353 497 632 855
Size: 51 302 144 135 223
Fragments arranged by size:
302 223 144 135 51
BmgBI CAC'GTC
Cuts at: 0 678 855
Size: 678 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 766 838 855
Size: 258 508 72 17
Fragments arranged by size:
508 258 72 17
Bpu10I CC'TnA_GC
Cuts at: 0 613 855
Size: 613 242
BsaHI Gr'CG_yC
Cuts at: 0 590 722 855
Size: 590 132 133
Fragments arranged by size:
590 133 132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 29 49 52 220 855
Size: 26 3 20 3 168 635
Fragments arranged by size:
635 168 26 20 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 808 855
Size: 24 784 47
Fragments arranged by size:
784 47 24
BsiHKAI G_wGCw'C
Cuts at: 0 188 353 605 650 855
Size: 188 165 252 45 205
Fragments arranged by size:
252 205 188 165 45
BsmI GAATG_Cn'
Cuts at: 0 311 855
Size: 311 544
BsmBI CGTCTCn'nnnn_
Cuts at: 0 742 855
Size: 742 113
Bsp1286I G_dGCh'C
Cuts at: 0 51 188 353 497 605 632 650 855
Size: 51 137 165 144 108 27 18 205
Fragments arranged by size:
205 165 144 137 108 51 27 18
BspHI T'CATG_A
Cuts at: 0 485 855
Size: 485 370
BsrBI CCG'CTC
Cuts at: 0 783 855
Size: 783 72
BsrFI r'CCGG_y
Cuts at: 0 552 855
Size: 552 303
BtsI GCAGTG_nn'
Cuts at: 0 217 855
Size: 217 638
EaeI y'GGCC_r
Cuts at: 0 582 713 855
Size: 582 131 142
Fragments arranged by size:
582 142 131
EarI CTCTTCn'nnn_
Cuts at: 0 517 755 855
Size: 517 238 100
Fragments arranged by size:
517 238 100
EcoICRI GAG'CTC
Cuts at: 0 648 855
Size: 648 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 766 838 855
Size: 258 508 72 17
Fragments arranged by size:
508 258 72 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 855
Size: 514 341
EcoO109I rG'GnC_Cy
Cuts at: 0 60 136 246 771 855
Size: 60 76 110 525 84
Fragments arranged by size:
525 110 84 76 60
FspI TGC'GCA
Cuts at: 0 27 855
Size: 27 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 407 439 855
Size: 256 32 119 32 416
Fragments arranged by size:
416 256 119 32 32
MslI CAynn'nnrTG
Cuts at: 0 490 855
Size: 490 365
MspA1I CmG'CkG
Cuts at: 0 387 855
Size: 387 468
NaeI GCC'GGC
Cuts at: 0 554 855
Size: 554 301
NgoMIV G'CCGG_C
Cuts at: 0 552 855
Size: 552 303
NspI r_CATG'y
Cuts at: 0 828 855
Size: 828 27
PasI CC'CwG_GG
Cuts at: 0 51 855
Size: 51 804
PfoI T'CCnGG_A
Cuts at: 0 515 671 855
Size: 515 156 184
Fragments arranged by size:
515 184 156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 587 619 855
Size: 587 32 236
Fragments arranged by size:
587 236 32
PpuMI rG'GwC_Cy
Cuts at: 0 136 246 855
Size: 136 110 609
Fragments arranged by size:
609 136 110
PstI C_TGCA'G
Cuts at: 0 224 855
Size: 224 631
SacI G_AGCT'C
Cuts at: 0 650 855
Size: 650 205
SanDI GG'GwC_CC
Cuts at: 0 136 855
Size: 136 719
SfcI C'TryA_G
Cuts at: 0 220 855
Size: 220 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 855
Size: 414 441
XmnI GAAnn'nnTTC
Cuts at: 0 199 364 855
Size: 199 165 491
Fragments arranged by size:
491 199 165
Enzymes that do cut:
AccI AflIII AhdI ApaLI AvaI BanII BbvCI BciVI
Bme1580I BmgBI BpmI Bpu10I BsaHI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MslI
MspA1I NaeI NgoMIV NspI PasI PfoI PpiI PpuMI
PstI SacI SanDI SfcI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiEI
BsiWI BspEI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI ZraI