Restriction map for
pFN21AB9387
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BanI BanII BbeI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaBI BseAI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 834 GGCGTC G[GA]^CG[CT]C
AspI 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 335 TGCGCA TGC^GCA
BamHI 440 GGATCC G^GATCC
BbrPI 64 CACGTG CAC^GTG
BclI 797 TGATCA T^GATCA
BglI 175 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 834 GGCGTC G[GA]^CG[CT]C
BsaI 613 GAGACC GGTCTC(1/5)
BsaWI 437 ACCGGA [AT]^CCGG[AT]
BsgI 631 CTGCAC GTGCAG(16/14)
BsiEI 281 CGATCG CG[GA][CT]^CG
BsmBI 583 GAGACG CGTCTC(1/5)
BspHI 794 TCATGA T^CATGA
BsrBI 507 GAGCGG CCGCTC(-3/-3)
DsaI 326 CCGCGG C^C[GA][CT]GG
FauI 509 GCGGG CCCGC(4/6)
FbaI 797 TGATCA T^GATCA
FspI 335 TGCGCA TGC^GCA
Hin1I 834 GGCGTC G[GA]^CG[CT]C
HphI 156 GGTGA GGTGA(8/7)
KspI 326 CCGCGG CCGC^GG
MwoI 175 GCCATCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 497 ACATGC [GA]CATG^[CT]
PflFI 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 78 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 64 CACGTG CAC^GTG
PmlI 64 CACGTG CAC^GTG
PstI 233 CTGCAG CTGCA^G
PvuI 281 CGATCG CGAT^CG
RcaI 794 TCATGA T^CATGA
SacII 326 CCGCGG CCGC^GG
SfcI 233 CTGCAG C^T[GA][CT]AG
SmlI 14 CTCAAG C^T[CT][GA]AG
Tsp509I 272 AATT ^AATT
Tth111I 405 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 78 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 417 GTAC GT^AC
699 GTAC
AlwNI 214 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
565 CAGGCGCTG
BalI 518 TGGCCA TGG^CCA
653 TGGCCA
BsaAI 64 CACGTG [CT]AC^GT[GA]
724 TACGTG
BseRI 52 GAGGAG GAGGAG(10/8)
304 GAGGAG
BsmAI 583 GAGAC GTCTC(1/5)
613 GAGAC
BspMI 332 ACCTGC ACCTGC(4/8)
153 GCAGGT
BstNI 180 CCTGG CC^[AT]GG
672 CCAGG
EarI 49 GAAGAG CTCTTC(1/4)
109 GAAGAG
EcoRII 180 CCTGG ^CC[AT]GG
672 CCAGG
HaeII 172 AGCGCC [GA]GCGC^[CT]
567 GGCGCT
HgaI 852 GACGC GACGC(5/10)
835 GCGTC
HinfI 12 GACTC G^A[ACGT]TC
547 GACTC
Ksp632I 49 GAAGAG CTCTTC(1/4)
109 GAAGAG
MluNI 518 TGGCCA TGG^CCA
653 TGGCCA
MscI 518 TGGCCA TGG^CCA
653 TGGCCA
MslI 537 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
651 CATGGCCATG
MvaI 180 CCTGG CC^[AT]GG
672 CCAGG
PleI 12 GACTC GAGTC(4/5)
547 GACTC
RsaI 417 GTAC GT^AC
699 GTAC
TaqI 284 TCGA T^CGA
695 TCGA
TthHB8I 284 TCGA T^CGA
695 TCGA
XcmI 209 CCAAACAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAACGAAGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbsI BbvI BcnI BpmI BpuAI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstUI BstYI Cac8I
CfoI Cfr13I DpnII EaeI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboI MboII
MflI MnlI MspA1I MspI MvnI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI TspRI
XhoII