Restriction map for
pFN21AB9387
(Circular) (Six-base) MAPSORT of: pFN21AB9387.seq Check: 7289 from: 1 to: 5569
pFN21AB9387 5569 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2149
Size: 5569
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3632 5016 5284 1632
Size: 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2312
Size: 32 5537
AlwNI CAG_nnn'CTG
Cuts at: 2224 2575 3312 4884 2224
Size: 351 737 1572 2909
Fragments arranged by size:
2909 1572 737 351
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 1870
Size: 73 951 4545
Fragments arranged by size:
4545 951 73
BamHI G'GATC_C
Cuts at: 2445 2899 3266 2445
Size: 454 367 4748
Fragments arranged by size:
4748 454 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 4345 618
Size: 325 217 627 1103 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2888 1391
Size: 1497 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2258 2505 928
Size: 971 317 42 247 3992
Fragments arranged by size:
3992 971 317 247 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 2802 1548
Size: 1254 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2186 4180 136
Size: 107 122 71 631 605 514 1994 1525
Fragments arranged by size:
1994 1525 631 605 514 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 3630 4786 1790
Size: 1840 1156 2573
Fragments arranged by size:
2573 1840 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2407 3300 4258
Size: 416 53 83 399 24 990 893 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 990 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2480 2528 2636 4229 1239
Size: 1007 234 48 108 1593 2579
Fragments arranged by size:
2579 1593 1007 234 108 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2004 3694 4559 4857 2004
Size: 1690 865 298 2716
Fragments arranged by size:
2716 1690 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 4232 882
Size: 617 1113 1620 2219
Fragments arranged by size:
2219 1620 1113 617
BsaAI yAC'GTr
Cuts at: 493 1204 2071 2731 3360 493
Size: 711 867 660 629 2702
Fragments arranged by size:
2702 867 711 660 629
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2840 3376 3758 4393
Size: 53 83 186 936 1307 536 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 1307 936 635 536 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2442 4002 4400 4674 4821 5232 2442
Size: 1560 398 274 147 411 2779
Fragments arranged by size:
2779 1560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 1178
Size: 30 165 30 909 30 4405
Fragments arranged by size:
4405 909 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2324 1186
Size: 886 252 4431
Fragments arranged by size:
4431 886 252
BseYI C'CCAG_C
Cuts at: 2355 2824 2860 4772 5268 2355
Size: 469 36 1912 496 2656
Fragments arranged by size:
2656 1912 496 469 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 1163
Size: 573 885 4111
Fragments arranged by size:
4111 885 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2289 3019 3780 4808 1540
Size: 275 186 288 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 288 275 186
BsiHKAI G_wGCw'C
Cuts at: 3630 3715 4786 3630
Size: 85 1071 4413
Fragments arranged by size:
4413 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
BsmBI CGTCTCn'nnnn_
Cuts at: 2582 2582
Size: 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2888 3630 3715 4786 1391
Size: 399 1098 742 85 1071 2174
Fragments arranged by size:
2174 1098 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 2799 3458 5188 2799
Size: 659 1730 3180
Fragments arranged by size:
3180 1730 659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 2346 2924 844
Size: 1305 197 578 3489
Fragments arranged by size:
3489 1305 578 197
BsrBI CCG'CTC
Cuts at: 1394 1823 2514 3456 1394
Size: 429 691 942 3507
Fragments arranged by size:
3507 942 691 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2118 2230 2445 2899 3266 3651 3668 5120
Size: 146 112 215 454 367 385 17 1452
Cuts at: 5120 5206 5218 5564 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 454 385 367 346 215 146
112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 3751
Fragments arranged by size:
3751 1266 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 2769 3085 3879 3899 2235
Size: 534 316 794 20 3905
Fragments arranged by size:
3905 794 534 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2256 2523
Size: 54 1425 50 275 18 357 69 267
Cuts at: 2523 2658 3016 3905 5534 8
Size: 135 358 889 1629 43
Fragments arranged by size:
1629 1425 889 358 357 275 267 135
69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2109 3499 1353
Size: 696 60 1390 3423
Fragments arranged by size:
3423 1390 696 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2480 2528 2636 3632 4229 5016
Size: 393 614 234 48 108 996 597 787
Cuts at: 5016 5284 1239
Size: 268 1524
Fragments arranged by size:
1524 996 787 614 597 393 268 234
108 48
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 2342 4075 2342
Size: 1733 3836
HaeII r_GCGC'y
Cuts at: 1678 2181 2576 4716 1678
Size: 503 395 2140 2531
Fragments arranged by size:
2531 2140 503 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 4210 4242 4284
Size: 32 135 32 554 32 2052 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 2052 554 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 4683 4867 1305
Size: 819 2559 184 2007
Fragments arranged by size:
2559 2007 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 2660 10
Size: 54 1425 343 693 135 2919
Fragments arranged by size:
2919 1425 693 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2546 2660 3527 3886 4045 518
Size: 648 1380 114 867 359 159 2042
Fragments arranged by size:
2042 1380 867 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2333 2433 2452 2985 3662 4810 5055 1606
Size: 727 100 19 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 727 677 533 245 100 19
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2506 2927 4472 1132
Size: 97 1277 421 1545 2229
Fragments arranged by size:
2229 1545 1277 421 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 1108
Size: 981 4588
PfoI T'CCnGG_A
Cuts at: 2372 2372
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PmlI CAC'GTG
Cuts at: 2071 2071
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2312 2344 3638 3670 5151 5183 2312
Size: 32 1294 32 1481 32 2698
Fragments arranged by size:
2698 1481 1294 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2242 2921 830
Size: 1412 679 3478
Fragments arranged by size:
3478 1412 679
PvuI CG_AT'CG
Cuts at: 2001 2289 2001
Size: 288 5281
SacII CC_GC'GG
Cuts at: 2334 2334
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2238 2917 4052 4733 4924 826
Size: 219 1193 679 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 681 679 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2019 3673 4574 4836 820
Size: 197 926 76 1654 901 262 1553
Fragments arranged by size:
1654 1553 926 901 262 197 76
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 3008 513
Size: 552 1943 3074
Fragments arranged by size:
3074 1943 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 2259 2411 3596 3613 3766 3951
Size: 1255 629 50 152 1185 17 153 185
Cuts at: 3951 4396 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1185 629 445 185 153 152
50 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2703 3815 5527
Size: 274 80 33 51 1443 726 1112 1712
Cuts at: 5527 96
Size: 138
Fragments arranged by size:
1712 1443 1112 726 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5529 5561 5529
Size: 32 5537
Tth111I GACn'n_nGTC
Cuts at: 1316 2413 1316
Size: 1097 4472
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2491 2221
Size: 270 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI