Restriction map for
pFN21AB9387
(Linear) (Six-base) MAPSORT of: KIBB9387.seq Check: 445 from: 1 to: 864
KIBB9387.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 864
Size: 144 720
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 864
Size: 307 32 525
Fragments arranged by size:
525 307 32
AlwNI CAG_nnn'CTG
Cuts at: 0 219 570 864
Size: 219 351 294
Fragments arranged by size:
351 294 219
BamHI G'GATC_C
Cuts at: 0 440 864
Size: 440 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 253 500 864
Size: 211 42 247 364
Fragments arranged by size:
364 247 211 42
BclI T'GATC_A
Cuts at: 0 797 864
Size: 797 67
BglI GCCn_nnn'nGGC
Cuts at: 0 181 864
Size: 181 683
BmrI ACTGGGnnnn_n'
Cuts at: 0 402 864
Size: 402 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 475 523 631 864
Size: 241 234 48 108 233
Fragments arranged by size:
241 234 233 108 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 864
Size: 607 257
BsaAI yAC'GTr
Cuts at: 0 66 726 864
Size: 66 660 138
Fragments arranged by size:
660 138 66
BsaHI Gr'CG_yC
Cuts at: 0 835 864
Size: 835 29
BsaWI w'CCGG_w
Cuts at: 0 437 864
Size: 437 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 864
Size: 307 30 527
Fragments arranged by size:
527 307 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 319 864
Size: 67 252 545
Fragments arranged by size:
545 252 67
BseYI C'CCAG_C
Cuts at: 0 350 819 855 864
Size: 350 469 36 9
Fragments arranged by size:
469 350 36 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 864
Size: 616 248
BsiEI CG_ry'CG
Cuts at: 0 284 864
Size: 284 580
BsmBI CGTCTCn'nnnn_
Cuts at: 0 577 864
Size: 577 287
BspHI T'CATG_A
Cuts at: 0 794 864
Size: 794 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 341 864
Size: 144 197 523
Fragments arranged by size:
523 197 144
BsrBI CCG'CTC
Cuts at: 0 509 864
Size: 509 355
BstYI r'GATC_y
Cuts at: 0 113 225 440 864
Size: 113 112 215 424
Fragments arranged by size:
424 215 113 112
BtgI C'CryG_G
Cuts at: 0 326 864
Size: 326 538
BtsI GCAGTG_nn'
Cuts at: 0 230 764 864
Size: 230 534 100
Fragments arranged by size:
534 230 100
EaeI y'GGCC_r
Cuts at: 0 182 251 518 653 864
Size: 182 69 267 135 211
Fragments arranged by size:
267 211 182 135 69
EarI CTCTTCn'nnn_
Cuts at: 0 44 104 864
Size: 44 60 760
Fragments arranged by size:
760 60 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 475 523 631 864
Size: 241 234 48 108 233
Fragments arranged by size:
241 234 233 108 48
FspI TGC'GCA
Cuts at: 0 337 864
Size: 337 527
HaeII r_GCGC'y
Cuts at: 0 176 571 864
Size: 176 395 293
Fragments arranged by size:
395 293 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 864
Size: 121 32 711
Fragments arranged by size:
711 121 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 864
Size: 119 745
MscI TGG'CCA
Cuts at: 0 520 655 864
Size: 520 135 209
Fragments arranged by size:
520 209 135
MslI CAynn'nnrTG
Cuts at: 0 541 655 864
Size: 541 114 209
Fragments arranged by size:
541 209 114
MspA1I CmG'CkG
Cuts at: 0 328 428 447 864
Size: 328 100 19 417
Fragments arranged by size:
417 328 100 19
NspI r_CATG'y
Cuts at: 0 501 864
Size: 501 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 864
Size: 84 780
PfoI T'CCnGG_A
Cuts at: 0 367 864
Size: 367 497
PmlI CAC'GTG
Cuts at: 0 66 864
Size: 66 798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 864
Size: 307 32 525
Fragments arranged by size:
525 307 32
PstI C_TGCA'G
Cuts at: 0 237 864
Size: 237 627
PvuI CG_AT'CG
Cuts at: 0 284 864
Size: 284 580
SacII CC_GC'GG
Cuts at: 0 329 864
Size: 329 535
SfcI C'TryA_G
Cuts at: 0 233 864
Size: 233 631
SmlI C'TyrA_G
Cuts at: 0 14 864
Size: 14 850
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 254 406 864
Size: 204 50 152 458
Fragments arranged by size:
458 204 152 50
TatI w'GTAC_w
Cuts at: 0 698 864
Size: 698 166
Tth111I GACn'n_nGTC
Cuts at: 0 408 864
Size: 408 456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 486 864
Size: 216 270 378
Fragments arranged by size:
378 270 216
Enzymes that do cut:
AarI AloI AlwNI BamHI BbsI BclI BglI BmrI
BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsmBI BspHI BspMI BsrBI BstYI BtgI
BtsI EaeI EarI Eco57MI FspI HaeII Hin4I MmeI
MscI MslI MspA1I NspI PflMI PfoI PmlI PpiI
PstI PvuI SacII SfcI SmlI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaBI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI XbaI XhoI
XmaI XmnI ZraI