Restriction map for
pFN21AB5659
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbsI BbvCI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 365 GACGTC GACGT^C
AcsI 499 GAATTC [GA]^AATT[CT]
AcyI 365 GACGTC G[GA]^CG[CT]C
AfaI 785 GTAC GT^AC
AlwNI 379 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 821 GGGCCC GGGCC^C
ApoI 499 GAATTC [GA]^AATT[CT]
AvaII 51 GGTCC G^G[AT]CC
BamHI 259 GGATCC G^GATCC
BbvI 425 GCAGC GCAGC(8/12)
BcgI 241 CGAGATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 549 GTATCC GTATCC(6/5)
BcnI 117 CCGGG CC^[GC]GG
BfaI 445 CTAG C^TAG
BglII 86 AGATCT A^GATCT
BsaHI 365 GACGTC G[GA]^CG[CT]C
BsaI 733 GAGACC GGTCTC(1/5)
BsgI 125 GTGCAG GTGCAG(16/14)
BsmFI 582 GGGAC GGGAC(10/14)
Bsp1407I 784 TGTACA T^GTACA
BsrDI 168 GCAATG GCAATG(2/0)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BsrGI 784 TGTACA T^GTACA
BstXI 403 CCAGATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
DraII 820 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 114 CGGCCG [CT]^GGCC[GA]
EagI 114 CGGCCG C^GGCCG
EarI 64 GAAGAG CTCTTC(1/4)
EclXI 114 CGGCCG C^GGCCG
Eco52I 114 CGGCCG C^GGCCG
EcoO109I 820 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 499 GAATTC G^AATTC
Fnu4HI 425 GCAGC GC^[ACGT]GC
Hin1I 365 GACGTC G[GA]^CG[CT]C
HphI 475 GGTGA GGTGA(8/7)
ItaI 425 GCAGC GC^[ACGT]GC
Ksp632I 64 GAAGAG CTCTTC(1/4)
MaeI 445 CTAG C^TAG
MseI 458 TTAA T^TAA
MslI 238 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 112 GCCGGC GCC^GGC
NciI 117 CCGGG CC^[GC]GG
NgoMIV 112 GCCGGC G^CCGGC
NlaIII 792 CATG CATG^
PflMI 378 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 887 CAGCTG CAG^CTG
RsaI 785 GTAC GT^AC
SapI 64 GAAGAGC GCTCTTC(1/4)
SfcI 432 CTACAG C^T[GA][CT]AG
SmlI 655 CTCAAG C^T[CT][GA]AG
SspBI 784 TGTACA T^GTACA
SspI 538 AATATT AAT^ATT
StuI 187 AGGCCT AGG^CCT
TaqI 240 TCGA T^CGA
Tru9I 458 TTAA T^TAA
TseI 425 GCAGC G^C[AT]GC
TthHB8I 240 TCGA T^CGA
Van91I 378 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 260 GATCC GGATC(4/5)
259 GGATC
BpmI 492 CTCCAG CTGGAG(16/14)
703 CTGGAG
BseRI 838 GAGGAG GAGGAG(10/8)
841 GAGGAG
BsiEI 41 CGGTCG CG[GA][CT]^CG
114 CGGCCG
BsmAI 733 GAGAC GTCTC(1/5)
155 GTCTC
BstF5I 375 CATCC GGATG(2/0)
708 GGATG
BstYI 86 AGATCT [GA]^GATC[CT]
259 GGATCC
FokI 375 CATCC GGATG(9/13)
708 GGATG
HapII 113 CCGG C^CGG
117 CCGG
HpaII 113 CCGG C^CGG
117 CCGG
MflI 86 AGATCT [GA]^GATC[CT]
259 GGATCC
MspA1I 753 CCGCTG C[AC]G^C[GT]G
887 CAGCTG
MspI 113 CCGG C^CGG
117 CCGG
PleI 490 GACTC GAGTC(4/5)
584 GACTC
SacI 358 GAGCTC GAGCT^C
865 GAGCTC
TfiI 478 GATTC G^A[AT]TC
810 GAATC
Tsp45I 130 GTCAC ^GT[GC]AC
800 GTGAC
Tsp509I 500 AATT ^AATT
775 AATT
XhoII 86 AGATCT [GA]^GATC[CT]
259 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII HaeIII HhaI HinP1I HinfI
MaeII MaeIII MboI MboII MnlI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TspRI