(Circular) (Six-base) MAPSORT of: pFN21AB5659.seq Check: 651 from: 1 to: 5629 pFN21AB5659 5629 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2374 3439 278 Size: 53 83 186 936 838 1065 2468 Fragments arranged by size: 2468 1065 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2531 3692 5076 5344 1632 Size: 899 1161 1384 268 1917 Fragments arranged by size: 1917 1384 1161 899 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2265 1184 Size: 1081 4548 AlwNI CAG_nnn'CTG Cuts at: 2389 3372 4944 2389 Size: 983 1572 3074 Fragments arranged by size: 3074 1572 983 ApaI G_GGCC'C Cuts at: 2830 2830 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2504 2938 3164 3316 5425 1493 Size: 1011 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 1011 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2264 2959 3326 2264 Size: 695 367 4567 Fragments arranged by size: 4567 695 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 4405 618 Size: 325 217 627 1163 1455 1842 Fragments arranged by size: 1842 1455 1163 627 325 217 BanII G_rGCy'C Cuts at: 1391 2367 2830 2874 2948 1391 Size: 976 463 44 74 4072 Fragments arranged by size: 4072 976 463 74 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2235 2269 3820 3854 1903 Size: 34 298 34 1551 34 3678 Fragments arranged by size: 3678 1551 298 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2565 3521 4731 2565 Size: 956 1210 3463 Fragments arranged by size: 3463 1210 956 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2091 5624 2091 Size: 3533 2096 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2707 2830 3690 4846 1790 Size: 917 123 860 1156 2573 Fragments arranged by size: 2573 1156 917 860 123 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2327 3360 4318 Size: 416 53 83 399 24 910 1033 958 Cuts at: 4318 442 Size: 1753 Fragments arranged by size: 1753 1033 958 910 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2482 2729 4289 1239 Size: 1243 247 1560 2579 Fragments arranged by size: 2579 1560 1243 247 Bpu10I CC'TnA_GC Cuts at: 2075 2582 2075 Size: 507 5122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 3754 4619 4917 2645 Size: 1109 865 298 3357 Fragments arranged by size: 3357 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2732 4292 882 Size: 617 1233 1560 2219 Fragments arranged by size: 2219 1560 1233 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2371 3436 3818 4453 Size: 53 83 186 936 838 1065 382 635 Cuts at: 4453 275 Size: 1451 Fragments arranged by size: 1451 1065 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2858 2861 1186 Size: 1672 3 3954 Fragments arranged by size: 3954 1672 3 BseYI C'CCAG_C Cuts at: 4832 5328 4832 Size: 496 5133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 1163 Size: 573 415 4641 Fragments arranged by size: 4641 573 415 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2122 3079 3840 4868 1540 Size: 275 186 48 73 957 761 1028 2301 Fragments arranged by size: 2301 1028 957 761 275 186 73 48 BsiHKAI G_wGCw'C Cuts at: 2367 2874 2918 3690 3775 4846 2367 Size: 507 44 772 85 1071 3150 Fragments arranged by size: 3150 1071 772 507 85 44 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2707 2830 2874 2918 2948 3690 Size: 399 577 340 123 44 44 30 742 Cuts at: 3690 3775 4846 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 399 340 123 85 44 44 30 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2180 4124 4298 65 Size: 1183 932 1944 174 1396 Fragments arranged by size: 1944 1396 1183 932 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 4273 4460 1700 Size: 110 307 2156 187 2869 Fragments arranged by size: 2869 2156 307 187 110 BsrGI T'GTAC_A Cuts at: 96 2789 96 Size: 2693 2936 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2415 2415 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2091 2264 2959 3326 3711 3728 5180 5266 Size: 119 173 695 367 385 17 1452 86 Cuts at: 5266 5278 5624 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 695 385 367 346 173 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5077 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2293 3145 3939 3959 2293 Size: 852 794 20 3963 Fragments arranged by size: 3963 852 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 3076 3965 Size: 54 1425 50 275 18 289 957 889 Cuts at: 3965 5594 8 Size: 1629 43 Fragments arranged by size: 1629 1425 957 889 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 3076 1537 Size: 275 307 957 4090 Fragments arranged by size: 4090 957 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 3559 1353 Size: 711 1495 3423 Fragments arranged by size: 3423 1495 711 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2365 2872 2365 Size: 507 5122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2482 2531 2729 3692 4289 5076 5344 Size: 393 850 49 198 963 597 787 268 Cuts at: 5344 1239 Size: 1524 Fragments arranged by size: 1524 963 850 787 597 393 268 198 49 EcoO109I rG'GnC_Cy Cuts at: 2826 2826 Size: 5629 EcoRI G'AATT_C Cuts at: 2504 2938 3316 2504 Size: 434 378 4817 Fragments arranged by size: 4817 434 378 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2256 2288 2294 2326 2361 Size: 32 135 32 684 32 6 32 35 Cuts at: 2361 2393 4270 4302 4344 4376 1373 Size: 32 1877 32 42 32 2626 Fragments arranged by size: 2626 1877 684 135 42 35 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2392 4743 4927 1305 Size: 1071 16 2351 184 2007 Fragments arranged by size: 2351 2007 1071 184 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 3587 3946 4105 518 Size: 648 1081 1340 359 159 2042 Fragments arranged by size: 2042 1340 1081 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2760 2894 3045 3722 4870 5115 1606 Size: 1154 134 151 677 1148 245 2120 Fragments arranged by size: 2120 1154 1148 677 245 151 134 NaeI GCC'GGC Cuts at: 2119 2119 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5077 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NgoMIV G'CCGG_C Cuts at: 2117 2117 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 1108 Size: 1281 4348 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2826 2826 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2894 2894 Size: 5629 SacI G_AGCT'C Cuts at: 2367 2874 2367 Size: 507 5122 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SapI GCTCTTCn'nnn_ Cuts at: 2064 2064 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2437 2977 4112 4793 4984 826 Size: 219 1392 540 1135 681 191 1471 Fragments arranged by size: 1471 1392 1135 681 540 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 3733 4634 4896 820 Size: 197 926 717 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 717 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 2545 3553 5 Size: 47 2493 1008 2081 Fragments arranged by size: 2493 2081 1008 47 StuI AGG'CCT Cuts at: 1517 2194 1517 Size: 677 4952 StyI C'CwwG_G Cuts at: 513 1065 3068 513 Size: 552 2003 3074 Fragments arranged by size: 3074 2003 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2756 3656 3673 3826 4011 4456 325 Size: 1255 1176 900 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1176 900 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2789 3875 5587 Size: 274 80 33 51 1443 812 1086 1712 Cuts at: 5587 96 Size: 138 Fragments arranged by size: 1712 1443 1086 812 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2372 3437 276 Size: 53 83 186 936 838 1065 2468 Fragments arranged by size: 2468 1065 936 838 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI