(Circular) (Six-base) MAPSORT of: pFN21AB5646.seq Check: 1669 from: 1 to: 6169 pFN21AB5646 6169 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3282 4232 5616 5884 1632 Size: 1650 950 1384 268 1917 Fragments arranged by size: 1917 1650 1384 950 268 AflII C'TTAA_G Cuts at: 820 1017 2719 820 Size: 197 1702 4270 Fragments arranged by size: 4270 1702 197 AflIII A'CryG_T Cuts at: 1203 2335 5068 6045 1203 Size: 1132 2733 977 1327 Fragments arranged by size: 2733 1327 1132 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 3912 5484 3912 Size: 1572 4597 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 2187 2394 2705 3478 3704 3856 5965 1493 Size: 694 207 311 773 226 152 2109 1697 Fragments arranged by size: 2109 1697 773 694 311 226 207 152 AseI AT'TA_AT Cuts at: 160 1032 2634 2927 4723 160 Size: 872 1602 293 1796 1606 Fragments arranged by size: 1796 1606 1602 872 293 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2054 2084 3453 3494 1870 Size: 73 93 18 30 1369 41 4545 Fragments arranged by size: 4545 1369 93 73 41 30 18 BamHI G'GATC_C Cuts at: 3055 3499 3866 3055 Size: 444 367 5358 Fragments arranged by size: 5358 444 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 3490 4945 618 Size: 325 217 627 680 1023 1455 1842 Fragments arranged by size: 1842 1455 1023 680 627 325 217 BanII G_rGCy'C Cuts at: 1391 2231 3488 1391 Size: 840 1257 4072 Fragments arranged by size: 4072 1257 840 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4780 136 Size: 107 122 71 631 605 337 2771 1525 Fragments arranged by size: 2771 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 2075 3586 2075 Size: 1511 4658 Bme1580I G_kGCm'C Cuts at: 1790 4230 5386 1790 Size: 2440 1156 2573 Fragments arranged by size: 2573 2440 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3900 4858 442 Size: 416 53 83 399 24 2483 958 1753 Fragments arranged by size: 2483 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2384 1056 Size: 1328 4841 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2313 2407 2886 4829 1239 Size: 1074 94 479 1943 2579 Fragments arranged by size: 2579 1943 1074 479 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2250 2668 4294 5159 5457 2250 Size: 418 1626 865 298 2962 Fragments arranged by size: 2962 1626 865 418 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3960 493 Size: 711 2756 2702 Fragments arranged by size: 2756 2702 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 4358 4993 275 Size: 53 83 186 936 2443 382 635 1451 Fragments arranged by size: 2443 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2979 3009 1178 Size: 30 165 30 1576 30 4338 Fragments arranged by size: 4338 1576 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3104 3312 1186 Size: 1918 208 4043 Fragments arranged by size: 4043 1918 208 BseYI C'CCAG_C Cuts at: 2740 5372 5868 2740 Size: 2632 496 3041 Fragments arranged by size: 3041 2632 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2752 2829 3063 1163 Size: 573 1016 77 234 4269 Fragments arranged by size: 4269 1016 573 234 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 4230 4315 5386 2076 Size: 2154 85 1071 2859 Fragments arranged by size: 2859 2154 1071 85 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2231 3488 4230 4315 5386 1391 Size: 399 286 155 1257 742 85 1071 2174 Fragments arranged by size: 2174 1257 1071 742 399 286 155 85 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 844 Size: 2680 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2017 4056 1394 Size: 429 194 2039 3507 Fragments arranged by size: 3507 2039 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3435 4813 5000 1700 Size: 110 1625 1378 187 2869 Fragments arranged by size: 2869 1625 1378 187 110 BsrGI T'GTAC_A Cuts at: 96 2818 96 Size: 2722 3447 BssHII G'CGCG_C Cuts at: 1925 2098 1925 Size: 173 5996 BssSI C'ACGA_G Cuts at: 4229 5241 4229 Size: 1012 5157 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6169 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstYI r'GATC_y Cuts at: 1972 3055 3203 3499 3866 4251 4268 5720 5806 Size: 1083 148 296 367 385 17 1452 86 Cuts at: 5806 5818 6164 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 296 148 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3417 3417 Size: 6169 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4678 Fragments arranged by size: 4678 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 2764 3685 4479 4499 2764 Size: 921 794 20 4434 Fragments arranged by size: 4434 921 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 2331 3473 2331 Size: 1142 5027 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3122 3616 4505 Size: 54 1425 50 275 18 1292 494 889 Cuts at: 4505 6134 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1292 889 494 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2119 2722 4099 1353 Size: 766 603 1377 3423 Fragments arranged by size: 3423 1377 766 603 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4756 5130 5276 387 Size: 1064 138 438 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2407 2886 3282 4232 4829 5616 Size: 393 681 94 479 396 950 597 787 Cuts at: 5616 5884 1239 Size: 268 1524 Fragments arranged by size: 1524 950 787 681 597 479 396 393 268 94 EcoNI CCTnn'n_nnAGG Cuts at: 2601 3420 2601 Size: 819 5350 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 EcoRV GAT'ATC Cuts at: 2499 2499 Size: 6169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2576 2576 Size: 6169 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 5316 1678 Size: 3638 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2292 2324 3188 3220 3400 Size: 32 135 32 720 32 864 32 180 Cuts at: 3400 3432 4810 4842 4884 4916 1373 Size: 32 1378 32 42 32 2626 Fragments arranged by size: 2626 1378 864 720 180 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2436 2729 3142 5283 5467 1305 Size: 1131 293 413 2141 184 2007 Fragments arranged by size: 2141 2007 1131 413 293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3124 10 Size: 54 1425 343 1292 3055 Fragments arranged by size: 3055 1425 1292 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4127 4486 4645 518 Size: 648 2961 359 159 2042 Fragments arranged by size: 2961 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 3585 4262 5410 5655 1606 Size: 473 1506 677 1148 245 2120 Fragments arranged by size: 2120 1506 1148 677 473 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4678 Fragments arranged by size: 4678 939 552 NdeI CA'TA_TG Cuts at: 387 2476 387 Size: 2089 4080 NheI G'CTAG_C Cuts at: 1052 2380 1052 Size: 1328 4841 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 2291 1108 Size: 1183 4986 PfoI T'CCnGG_A Cuts at: 2148 2148 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 2193 3739 2193 Size: 1546 4623 PstI C_TGCA'G Cuts at: 830 2123 3521 830 Size: 1293 1398 3478 Fragments arranged by size: 3478 1398 1293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SapI GCTCTTCn'nnn_ Cuts at: 2119 2119 Size: 6169 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2119 3517 4652 5333 5524 826 Size: 219 1074 1398 1135 681 191 1471 Fragments arranged by size: 1471 1398 1135 1074 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 2038 2056 2086 3496 1872 Size: 166 18 30 1410 4545 Fragments arranged by size: 4545 1410 166 30 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 2683 2719 4273 5174 5436 Size: 197 926 322 418 36 1554 901 262 Cuts at: 5436 820 Size: 1553 Fragments arranged by size: 1554 1553 926 901 418 322 262 197 36 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 3286 1517 Size: 1769 4400 StyI C'CwwG_G Cuts at: 513 1065 2004 2580 3608 513 Size: 552 939 576 1028 3074 Fragments arranged by size: 3074 1028 939 576 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4196 4213 4366 4551 4996 325 Size: 1255 2616 17 153 185 445 1498 Fragments arranged by size: 2616 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2657 2818 4415 Size: 274 80 33 51 1443 680 161 1597 Cuts at: 4415 6127 96 Size: 1712 138 Fragments arranged by size: 1712 1597 1443 680 274 161 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2743 2775 6129 6161 2743 Size: 32 3354 32 2751 Fragments arranged by size: 3354 2751 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3244 3244 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2036 2054 2084 3494 1870 Size: 166 18 30 1410 4545 Fragments arranged by size: 4545 1410 166 30 18 XmnI GAAnn'nnTTC Cuts at: 2394 4298 2394 Size: 1904 4265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI