Restriction map for
pFN21AB5635
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BseAI BsiEI
BsiHKAI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI KspI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1110 CGCG CG^CG
AvaII 241 GGACC G^G[AT]CC
BalI 231 TGGCCA TGG^CCA
BanI 263 GGTGCC G^G[CT][GA]CC
BbsI 694 GTCTTC GAAGAC(2/6)
BcgI 749 CGAAAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1083 GTATCC GTATCC(6/5)
BclI 1265 TGATCA T^GATCA
BcnI 81 CCGGG CC^[GC]GG
BfaI 791 CTAG C^TAG
BmyI 264 GTGCCC G[AGT]GC[ACT]^C
BpmI 1114 CTGGAG CTGGAG(16/14)
BpuAI 694 GTCTTC GAAGAC(2/6)
BsaBI 1266 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 548 ACCGGA [AT]^CCGG[AT]
BseRI 164 GAGGAG GAGGAG(10/8)
BsiWI 899 CGTACG C^GTACG
BsmFI 969 GGGAC GGGAC(10/14)
Bsp1286I 264 GTGCCC G[AGT]GC[ACT]^C
BsrBI 724 GAGCGG CCGCTC(-3/-3)
BssSI 89 CTCGTG CACGAG(-5/-1)
BstBI 1132 TTCGAA TT^CGAA
BstUI 1110 CGCG CG^CG
BstXI 671 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1111 GCGC GCG^C
DraI 1332 TTTAAA TTT^AAA
DrdI 304 GACTGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 937 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 514 ATGCAT ATGCA^T
FbaI 1265 TGATCA T^GATCA
HhaI 1111 GCGC GCG^C
HinP1I 1111 GCGC G^CGC
HincII 979 GTTAAC GT[CT]^[GA]AC
HindII 979 GTTAAC GT[CT]^[GA]AC
HpaI 979 GTTAAC GTT^AAC
MaeI 791 CTAG C^TAG
MaeII 902 ACGT A^CGT
MamI 1266 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 767 CAATTG C^AATTG
MluNI 231 TGGCCA TGG^CCA
MscI 231 TGGCCA TGG^CCA
MslI 309 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 767 CAATTG C^AATTG
MvnI 1110 CGCG CG^CG
NciI 81 CCGGG CC^[GC]GG
NsiI 514 ATGCAT ATGCA^T
NspV 1132 TTCGAA TT^CGAA
PflMI 331 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 730 CAGCTG CAG^CTG
SapI 772 GCTCTTC GCTCTTC(1/4)
SfcI 221 CTACAG C^T[GA][CT]AG
SfuI 1132 TTCGAA TT^CGAA
SmlI 1204 CTCAAG C^T[CT][GA]AG
Van91I 331 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsmI 1143 GAATGC GAATGC(1/-1)
516 GCATTC
BspHI 777 TCATGA T^CATGA
1014 TCATGA
BstYI 326 AGATCC [GA]^GATC[CT]
1200 GGATCT
EarI 773 CTCTTC CTCTTC(1/4)
1123 GAAGAG
FauI 425 CCCGC CCCGC(4/6)
1096 GCGGG
HapII 81 CCGG C^CGG
549 CCGG
HpaII 81 CCGG C^CGG
549 CCGG
Ksp632I 773 CTCTTC CTCTTC(1/4)
1123 GAAGAG
MflI 326 AGATCC [GA]^GATC[CT]
1200 GGATCT
MseI 980 TTAA T^TAA
1333 TTAA
MspI 81 CCGG C^CGG
549 CCGG
NspI 33 GCATGT [GA]CATG^[CT]
956 ACATGC
RcaI 777 TCATGA T^CATGA
1014 TCATGA
TaqI 201 TCGA T^CGA
1133 TCGA
Tru9I 980 TTAA T^TAA
1333 TTAA
Tsp45I 94 GTGAC ^GT[GC]AC
857 GTGAC
Tsp509I 204 AATT ^AATT
768 AATT
TthHB8I 201 TCGA T^CGA
1133 TCGA
XhoII 326 AGATCC [GA]^GATC[CT]
1200 GGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BsgI BsiYI BslI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI TspRI