(Circular) (Six-base) MAPSORT of: pFN21AB5635.seq Check: 4115 from: 1 to: 6127 pFN21AB5635 6127 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 5574 5842 1632 Size: 2558 1384 268 1917 Fragments arranged by size: 2558 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 3870 5442 3870 Size: 1572 4555 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2268 3448 4903 618 Size: 325 217 627 481 1180 1455 1842 Fragments arranged by size: 1842 1455 1180 627 481 325 217 BanII G_rGCy'C Cuts at: 1391 3446 1391 Size: 2055 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2692 928 Size: 971 793 4363 Fragments arranged by size: 4363 971 793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2743 2777 4318 4352 1903 Size: 34 806 34 1541 34 3678 Fragments arranged by size: 3678 1541 806 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3099 4019 5229 3099 Size: 920 1210 3997 Fragments arranged by size: 3997 1210 920 BclI T'GATC_A Cuts at: 1548 3270 1548 Size: 1722 4405 BfrBI ATG'CAT Cuts at: 2521 2521 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2273 4188 5344 1790 Size: 483 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 483 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2718 3858 4816 Size: 416 53 83 399 24 1301 1140 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1753 1301 1140 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3140 4787 1239 Size: 1901 1647 2579 Fragments arranged by size: 2579 1901 1647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3194 4252 5117 5415 3194 Size: 1058 865 298 3906 Fragments arranged by size: 3906 1058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 3275 6121 3275 Size: 2846 3281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 4560 4958 5232 5379 5790 2553 Size: 2007 398 274 147 411 2890 Fragments arranged by size: 2890 2007 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5902 Fragments arranged by size: 5902 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2184 1186 Size: 998 5129 BseYI C'CCAG_C Cuts at: 5330 5826 5330 Size: 496 5631 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 2296 2469 2557 1163 Size: 573 441 119 173 88 4733 Fragments arranged by size: 4733 573 441 173 119 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4188 4273 5344 4188 Size: 85 1071 4971 Fragments arranged by size: 4971 1071 85 BsiWI C'GTAC_G Cuts at: 2904 2904 Size: 6127 BsmI GAATG_Cn' Cuts at: 2521 3154 3638 3731 2521 Size: 633 484 93 4917 Fragments arranged by size: 4917 633 484 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 3446 4188 4273 5344 1391 Size: 399 483 1173 742 85 1071 2174 Fragments arranged by size: 2174 1173 1071 742 483 399 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 2782 3019 4016 5746 2782 Size: 237 997 1730 3163 Fragments arranged by size: 3163 1730 997 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2731 4014 1394 Size: 429 908 1283 3507 Fragments arranged by size: 3507 1283 908 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 2440 2701 2806 4622 4796 65 Size: 1183 1041 151 261 105 1816 174 1396 Fragments arranged by size: 1816 1396 1183 1041 261 174 151 105 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 2094 4187 5199 2094 Size: 2093 1012 3022 Fragments arranged by size: 3022 2093 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 3138 4931 3138 Size: 1793 4334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2683 2683 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2331 3205 3457 3824 4209 4226 5678 5764 Size: 359 874 252 367 385 17 1452 86 Cuts at: 5764 5776 6122 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 874 385 367 359 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5575 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 3246 3643 4437 4457 3246 Size: 397 794 20 4916 Fragments arranged by size: 4916 794 397 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3339 3431 3339 Size: 92 6035 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 5134 809 Size: 507 999 2819 1802 Fragments arranged by size: 2819 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 2685 3300 Size: 54 1425 50 275 18 406 449 615 Cuts at: 3300 3574 4463 6092 8 Size: 274 889 1629 43 Fragments arranged by size: 1629 1425 889 615 449 406 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2784 3123 4057 1353 Size: 1431 339 934 3423 Fragments arranged by size: 3423 1431 934 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3140 4190 4787 5574 5842 1239 Size: 393 1508 1050 597 787 268 1524 Fragments arranged by size: 1524 1508 1050 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2946 2946 Size: 6127 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2391 2423 3335 3367 4768 Size: 32 135 32 819 32 912 32 1401 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1401 912 819 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3471 3717 669 Size: 988 281 1048 485 246 3079 Fragments arranged by size: 3079 1048 988 485 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 2986 3717 2986 Size: 731 5396 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 2772 3726 2772 Size: 954 5173 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2447 3306 5241 5425 1305 Size: 1142 859 1935 184 2007 Fragments arranged by size: 2007 1935 1142 859 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 10 Size: 54 1425 343 406 3899 Fragments arranged by size: 3899 1425 406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 4085 4444 4603 518 Size: 648 1152 1767 359 159 2042 Fragments arranged by size: 2042 1767 1152 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2298 2737 3101 3543 4220 5368 5613 1606 Size: 692 439 364 442 677 1148 245 2120 Fragments arranged by size: 2120 1148 692 677 442 439 364 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5575 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2523 2523 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2042 2965 3485 5030 1132 Size: 97 813 923 520 1545 2229 Fragments arranged by size: 2229 1545 923 813 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 1108 Size: 1234 4893 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 2504 3697 2504 Size: 1193 4934 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2737 2737 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SapI GCTCTTCn'nnn_ Cuts at: 2784 2784 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2226 3475 4610 5291 5482 826 Size: 219 1181 1249 1135 681 191 1471 Fragments arranged by size: 1471 1249 1181 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3209 4231 5132 5394 820 Size: 197 926 1266 1022 901 262 1553 Fragments arranged by size: 1553 1266 1022 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 3566 513 Size: 552 2501 3074 Fragments arranged by size: 3074 2501 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4373 6085 96 Size: 274 80 33 51 1443 2396 1712 138 Fragments arranged by size: 2396 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI