(Circular) (Six-base) MAPSORT of: pFN21AB5607.seq Check: 5875 from: 1 to: 6100 pFN21AB5607 6100 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2414 2840 2414 Size: 426 5674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2535 2613 3443 1937 Size: 598 78 830 4594 Fragments arranged by size: 4594 830 598 78 Acc65I G'GTAC_C Cuts at: 2651 3421 2651 Size: 770 5330 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4163 5547 5815 1632 Size: 2531 1384 268 1917 Fragments arranged by size: 2531 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 2258 4999 5976 1203 Size: 1055 2741 977 1327 Fragments arranged by size: 2741 1327 1055 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 3372 4829 3372 Size: 1457 4643 AleI CACnn'nnGTG Cuts at: 1166 2571 2663 1166 Size: 1405 92 4603 Fragments arranged by size: 4603 1405 92 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2090 2999 3053 3843 5415 2090 Size: 909 54 790 1572 2775 Fragments arranged by size: 2775 1572 909 790 54 ApaI G_GGCC'C Cuts at: 2681 2681 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2607 2913 3314 3425 1870 Size: 73 664 306 401 111 4545 Fragments arranged by size: 4545 664 401 306 111 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2651 2858 3421 4876 618 Size: 325 217 627 864 207 563 1455 1842 Fragments arranged by size: 1842 1455 864 627 563 325 217 207 BanII G_rGCy'C Cuts at: 1391 2035 2271 2681 3219 3419 1391 Size: 644 236 410 538 200 4072 Fragments arranged by size: 4072 644 538 410 236 200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BbvCI CC'TCA_GC Cuts at: 2839 2839 Size: 6100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2702 2736 4291 4325 1903 Size: 34 765 34 1555 34 3678 Fragments arranged by size: 3678 1555 765 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3084 6095 3084 Size: 3011 3089 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2258 2681 4161 5317 1790 Size: 468 423 1480 1156 2573 Fragments arranged by size: 2573 1480 1156 468 423 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2626 3831 4789 Size: 416 53 83 399 24 1209 1205 958 Cuts at: 4789 442 Size: 1753 Fragments arranged by size: 1753 1209 1205 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2051 2707 2051 Size: 656 5444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4760 1239 Size: 3521 2579 Bpu10I CC'TnA_GC Cuts at: 2682 2839 3218 2682 Size: 157 379 5564 Fragments arranged by size: 5564 379 157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 3282 4763 882 Size: 617 1509 274 1481 2219 Fragments arranged by size: 2219 1509 1481 617 274 BsaAI yAC'GTr Cuts at: 493 1204 2573 3891 493 Size: 711 1369 1318 2702 Fragments arranged by size: 2702 1369 1318 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3907 4289 4924 275 Size: 53 83 186 936 2374 382 635 1451 Fragments arranged by size: 2374 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2101 4533 4931 5205 5352 5763 2101 Size: 2432 398 274 147 411 2438 Fragments arranged by size: 2438 2432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2497 2527 2700 2730 3018 Size: 30 165 30 1094 30 173 30 288 Cuts at: 3018 3048 1178 Size: 30 4230 Fragments arranged by size: 4230 1094 288 173 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2043 2147 3026 3227 1186 Size: 857 104 879 201 4059 Fragments arranged by size: 4059 879 857 201 104 BseYI C'CCAG_C Cuts at: 3369 5303 5799 3369 Size: 1934 496 3670 Fragments arranged by size: 3670 1934 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2392 2818 1163 Size: 573 656 426 4445 Fragments arranged by size: 4445 656 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 2271 2965 3219 4161 4246 5317 2035 Size: 236 694 254 942 85 1071 2818 Fragments arranged by size: 2818 1071 942 694 254 236 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 BsmBI CGTCTCn'nnnn_ Cuts at: 2782 2782 Size: 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2258 2271 2681 2965 3219 3419 Size: 399 245 223 13 410 284 254 200 Cuts at: 3419 4161 4246 5317 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 284 254 245 223 200 85 13 BspEI T'CCGG_A Cuts at: 2101 5763 2101 Size: 3662 2438 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2075 2414 2840 3455 844 Size: 1231 339 426 615 3489 Fragments arranged by size: 3489 1231 615 426 339 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2321 4595 4769 65 Size: 1183 1073 2274 174 1396 Fragments arranged by size: 2274 1396 1183 1073 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4744 4931 1700 Size: 110 2934 187 2869 Fragments arranged by size: 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 794 Size: 1526 4574 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstXI CCAn_nnnn'nTGG Cuts at: 2882 2882 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2542 2553 3084 3430 3797 4182 4199 5651 Size: 570 11 531 346 367 385 17 1452 Cuts at: 5651 5737 5749 6095 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 570 531 385 367 346 346 86 17 12 11 BstZ17I GTA'TAC Cuts at: 2614 2614 Size: 6100 Bsu36I CC'TnA_GG Cuts at: 3162 3162 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4609 Fragments arranged by size: 4609 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2727 3616 4410 4430 2070 Size: 657 889 794 20 3740 Fragments arranged by size: 3740 889 794 657 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2165 5107 809 Size: 507 849 2942 1802 Fragments arranged by size: 2942 1802 849 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3547 4436 Size: 54 1425 50 275 18 177 1540 889 Cuts at: 4436 6065 8 Size: 1629 43 Fragments arranged by size: 1629 1540 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4030 1353 Size: 2677 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2033 2269 3217 2033 Size: 236 948 4916 Fragments arranged by size: 4916 948 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4163 4760 5547 5815 1239 Size: 393 2531 597 787 268 1524 Fragments arranged by size: 2531 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2353 2353 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 2137 2620 2677 2678 2137 Size: 483 57 1 5559 Fragments arranged by size: 5559 483 57 1 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 3235 5247 1678 Size: 1557 2012 2531 Fragments arranged by size: 2531 2012 1557 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2545 2577 3024 3056 3358 Size: 32 135 32 973 32 447 32 302 Cuts at: 3358 3390 4741 4773 4815 4847 1373 Size: 32 1351 32 42 32 2626 Fragments arranged by size: 2626 1351 973 447 302 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2983 2989 3366 3444 3690 669 Size: 988 281 1045 6 377 78 246 3079 Fragments arranged by size: 3079 1045 988 377 281 246 78 6 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 2655 3425 2655 Size: 770 5330 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 2258 5976 2258 Size: 3718 2382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3087 5214 5398 1305 Size: 1782 2127 184 2007 Fragments arranged by size: 2127 2007 1782 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4101 Fragments arranged by size: 4101 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2074 2571 2663 2880 4058 4417 4576 Size: 648 908 497 92 217 1178 359 159 Cuts at: 4576 518 Size: 2042 Fragments arranged by size: 2042 1178 908 648 497 359 217 159 92 MspA1I CmG'CkG Cuts at: 1606 2448 2559 2760 2843 2996 3193 3516 4193 Size: 842 111 201 83 153 197 323 677 Cuts at: 4193 5341 5586 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 323 245 201 197 153 111 83 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4609 Fragments arranged by size: 4609 939 552 NdeI CA'TA_TG Cuts at: 387 3392 387 Size: 3005 3095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2217 2717 3131 3458 5003 1132 Size: 97 988 500 414 327 1545 2229 Fragments arranged by size: 2229 1545 988 500 414 327 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 2196 3180 2196 Size: 984 5116 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PmlI CAC'GTG Cuts at: 2573 2573 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2620 2137 Size: 483 5617 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3075 3670 3075 Size: 595 5505 PspOMI G'GGCC_C Cuts at: 2677 2677 Size: 6100 PspXI vC'TCGA_Gb Cuts at: 2607 2607 Size: 6100 PstI C_TGCA'G Cuts at: 830 2357 3452 830 Size: 1527 1095 3478 Fragments arranged by size: 3478 1527 1095 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2448 2760 2996 3193 2448 Size: 312 236 197 5355 Fragments arranged by size: 5355 312 236 197 SacI G_AGCT'C Cuts at: 2035 2271 3219 2035 Size: 236 948 4916 Fragments arranged by size: 4916 948 236 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SanDI GG'GwC_CC Cuts at: 2620 2620 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2353 2443 3095 3448 4583 5264 5455 Size: 219 1308 90 652 353 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1308 1135 681 652 353 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2915 3427 1872 Size: 1043 512 4545 Fragments arranged by size: 4545 1043 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 2607 4204 5105 5367 820 Size: 197 926 664 1597 901 262 1553 Fragments arranged by size: 1597 1553 926 901 664 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 2390 152 Size: 2238 3862 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2004 3539 513 Size: 552 939 1535 3074 Fragments arranged by size: 3074 1535 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2754 3300 4127 4144 4297 4482 4927 Size: 1255 1174 546 827 17 153 185 445 Cuts at: 4927 325 Size: 1498 Fragments arranged by size: 1498 1255 1174 827 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2779 4346 6058 Size: 274 80 33 51 1443 802 1567 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 1712 1567 1443 802 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2497 2529 2700 2732 6060 6092 2497 Size: 32 171 32 3328 32 2505 Fragments arranged by size: 3328 2505 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2331 3112 3142 2331 Size: 781 30 5289 Fragments arranged by size: 5289 781 30 XhoI C'TCGA_G Cuts at: 1943 2607 1943 Size: 664 5436 XmaI C'CCGG_G Cuts at: 1870 2913 3425 1870 Size: 1043 512 4545 Fragments arranged by size: 4545 1043 512 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI