(Circular) (Six-base) MAPSORT of: pFN21AB5557.seq Check: 7219 from: 1 to: 6097 pFN21AB5557 6097 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3229 4160 5544 5812 1632 Size: 1597 931 1384 268 1917 Fragments arranged by size: 1917 1597 1384 931 268 AfeI AGC'GCT Cuts at: 3204 3287 3204 Size: 83 6014 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2457 2483 4996 5973 1203 Size: 1254 26 2513 977 1327 Fragments arranged by size: 2513 1327 1254 977 26 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 3131 1166 Size: 1965 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaI G_GGCC'C Cuts at: 2218 2218 Size: 6097 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2646 2759 3406 3632 3784 5893 1493 Size: 1153 113 647 226 152 2109 1697 Fragments arranged by size: 2109 1697 1153 647 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2907 3422 1870 Size: 73 964 515 4545 Fragments arranged by size: 4545 964 515 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 3418 4873 618 Size: 325 217 627 624 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2218 2892 2949 3416 1391 Size: 624 203 674 57 467 4072 Fragments arranged by size: 4072 674 624 467 203 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 928 Size: 971 231 4895 Fragments arranged by size: 4895 971 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2222 2447 3989 5199 2222 Size: 225 1542 1210 3120 Fragments arranged by size: 3120 1542 1210 225 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2277 4708 136 Size: 107 122 71 631 605 605 2431 1525 Fragments arranged by size: 2431 1525 631 605 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2218 2558 2753 4158 5314 1790 Size: 428 340 195 1405 1156 2573 Fragments arranged by size: 2573 1405 1156 428 340 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2524 2815 2876 3059 3177 4757 1239 Size: 1285 291 61 183 118 1580 2579 Fragments arranged by size: 2579 1580 1285 291 183 118 61 Bpu10I CC'TnA_GC Cuts at: 3212 3212 Size: 6097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2205 3364 4222 5087 5385 2205 Size: 1159 858 865 298 2917 Fragments arranged by size: 2917 1159 865 858 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2697 4760 882 Size: 617 1198 2063 2219 Fragments arranged by size: 2219 2063 1198 617 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 1178 Size: 30 165 30 1005 30 4837 Fragments arranged by size: 4837 1005 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3020 1186 Size: 1834 4263 BseYI C'CCAG_C Cuts at: 2084 5300 5796 2084 Size: 3216 496 2385 Fragments arranged by size: 3216 2385 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2976 2979 1163 Size: 573 1240 3 4281 Fragments arranged by size: 4281 1240 573 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2512 3547 4308 5336 1540 Size: 275 186 511 1035 761 1028 2301 Fragments arranged by size: 2301 1035 1028 761 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2831 2892 4158 4243 5314 2177 Size: 654 61 1266 85 1071 2960 Fragments arranged by size: 2960 1266 1071 654 85 61 BsmI GAATG_Cn' Cuts at: 3090 3608 3701 3090 Size: 518 93 5486 Fragments arranged by size: 5486 518 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2177 2218 2558 2753 2831 2892 Size: 399 225 162 41 340 195 78 61 Cuts at: 2892 2949 3416 4158 4243 5314 1391 Size: 57 467 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 467 399 340 225 195 162 85 78 61 57 41 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2473 2508 3984 1394 Size: 429 650 35 1476 3507 Fragments arranged by size: 3507 1476 650 429 35 BsrDI GCAATG_nn' Cuts at: 65 1248 2574 4592 4766 65 Size: 1183 1326 2018 174 1396 Fragments arranged by size: 2018 1396 1326 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2511 3069 4741 4928 1700 Size: 110 701 558 1672 187 2869 Fragments arranged by size: 2869 1672 701 558 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2712 3427 3794 4179 4196 5648 5734 5746 Size: 740 715 367 385 17 1452 86 12 Cuts at: 5746 6092 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 740 715 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2168 3129 513 Size: 552 939 164 961 3481 Fragments arranged by size: 3481 961 939 552 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2374 487 Size: 683 56 1148 4210 Fragments arranged by size: 4210 1148 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2770 2805 529 Size: 35 2206 35 3821 Fragments arranged by size: 3821 2206 35 35 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DraIII CAC_nnn'GTG Cuts at: 3104 3104 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 5104 809 Size: 507 816 2972 1802 Fragments arranged by size: 2972 1802 816 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2509 2513 3544 Size: 54 1425 50 275 18 679 4 1031 Cuts at: 3544 4433 6062 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 679 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2509 3544 1537 Size: 275 697 1035 4090 Fragments arranged by size: 4090 1035 697 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2302 2623 3348 4027 1353 Size: 949 321 725 679 3423 Fragments arranged by size: 3423 949 725 679 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2890 2890 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2524 2815 2876 3059 3177 3229 4160 Size: 393 892 291 61 183 118 52 931 Cuts at: 4160 4757 5544 5812 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 931 892 787 597 393 291 268 183 118 61 52 EcoNI CCTnn'n_nnAGG Cuts at: 2223 2223 Size: 6097 EcoO109I rG'GnC_Cy Cuts at: 2214 2215 2214 Size: 1 6096 EcoRI G'AATT_C Cuts at: 2646 3406 3784 2646 Size: 760 378 4959 Fragments arranged by size: 4959 760 378 FseI GG_CCGG'CC Cuts at: 2515 2515 Size: 6097 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 3206 3289 5244 1678 Size: 1528 83 1955 2531 Fragments arranged by size: 2531 1955 1528 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3175 3207 4738 4770 4812 Size: 32 135 32 1603 32 1531 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1603 1531 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2712 2946 5211 5395 1305 Size: 1407 234 2265 184 2007 Fragments arranged by size: 2265 2007 1407 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2486 3128 3131 4055 4414 4573 Size: 648 1020 300 642 3 924 359 159 Cuts at: 4573 518 Size: 2042 Fragments arranged by size: 2042 1020 924 648 642 359 300 159 3 MspA1I CmG'CkG Cuts at: 1606 2993 3513 4190 5338 5583 1606 Size: 1387 520 677 1148 245 2120 Fragments arranged by size: 2120 1387 1148 677 520 245 NaeI GCC'GGC Cuts at: 2513 2513 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NgoMIV G'CCGG_C Cuts at: 2511 2511 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2487 3455 5000 1132 Size: 97 1258 968 1545 2229 Fragments arranged by size: 2229 1545 1258 968 97 PasI CC'CwG_GG Cuts at: 1348 2245 1348 Size: 897 5200 PciI A'CATG_T Cuts at: 2483 4996 2483 Size: 2513 3584 PflMI CCAn_nnn'nTGG Cuts at: 1108 2387 2813 1108 Size: 1279 426 4392 Fragments arranged by size: 4392 1279 426 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2214 2214 Size: 6097 PstI C_TGCA'G Cuts at: 830 2213 3093 3210 3449 830 Size: 1383 880 117 239 3478 Fragments arranged by size: 3478 1383 880 239 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2993 2993 Size: 6097 RsrII CG'GwC_CG Cuts at: 2475 2475 Size: 6097 SacI G_AGCT'C Cuts at: 2892 2892 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2302 2302 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SexAI A'CCwGG_T Cuts at: 2252 2252 Size: 6097 SfcI C'TryA_G Cuts at: 826 1045 2209 3089 3206 3445 4580 5261 5452 Size: 219 1164 880 117 239 1135 681 191 Cuts at: 5452 826 Size: 1471 Fragments arranged by size: 1471 1164 1135 880 681 239 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2220 3343 4201 5102 5364 820 Size: 197 926 277 1123 858 901 262 1553 Fragments arranged by size: 1553 1123 926 901 858 277 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 2501 2673 1517 Size: 984 172 4941 Fragments arranged by size: 4941 984 172 StyI C'CwwG_G Cuts at: 513 1065 2004 3536 513 Size: 552 939 1532 3074 Fragments arranged by size: 3074 1532 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2966 3061 3134 4124 4141 4294 4479 Size: 1255 1386 95 73 990 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1386 1255 990 445 185 153 95 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 2718 4343 Size: 274 80 33 51 1443 228 513 1625 Cuts at: 4343 6055 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 513 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 2339 1316 Size: 1023 5074 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacII SanDI SfiI SfoI SrfI SwaI XcmI