(Circular) (Six-base) MAPSORT of: pFN21AB5539.seq Check: 9195 from: 1 to: 6388 pFN21AB5539 6388 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 2934 3731 1937 Size: 72 925 797 4594 Fragments arranged by size: 4594 925 797 72 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2871 2887 3072 3221 3513 4451 5835 6103 Size: 1239 16 185 149 292 938 1384 268 Cuts at: 6103 1632 Size: 1917 Fragments arranged by size: 1917 1384 1239 938 292 268 185 149 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 3525 5287 6264 1203 Size: 2322 1762 977 1327 Fragments arranged by size: 2322 1762 1327 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AlwNI CAG_nnn'CTG Cuts at: 2359 2381 3641 4131 5703 2359 Size: 22 1260 490 1572 3044 Fragments arranged by size: 3044 1572 1260 490 22 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 3697 3923 4075 6184 1493 Size: 2204 226 152 2109 1697 Fragments arranged by size: 2204 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 3123 3713 1870 Size: 73 1180 590 4545 Fragments arranged by size: 4545 1180 590 73 BamHI G'GATC_C Cuts at: 2070 2970 3718 4085 2070 Size: 900 748 367 4373 Fragments arranged by size: 4373 900 748 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3134 3709 5164 618 Size: 325 217 627 1347 575 1455 1842 Fragments arranged by size: 1842 1455 1347 627 575 325 217 BanII G_rGCy'C Cuts at: 1391 3124 3217 3596 3707 1391 Size: 1733 93 379 111 4072 Fragments arranged by size: 4072 1733 379 111 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2679 3060 3501 928 Size: 971 780 381 441 3815 Fragments arranged by size: 3815 971 780 441 381 BbvCI CC'TCA_GC Cuts at: 3626 3626 Size: 6388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4280 5490 3467 Size: 813 1210 4365 Fragments arranged by size: 4365 1210 813 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4999 136 Size: 107 122 71 631 605 3327 1525 Fragments arranged by size: 3327 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2405 3366 3408 6383 2405 Size: 961 42 2975 2410 Fragments arranged by size: 2975 2410 961 42 BlpI GC'TnA_GC Cuts at: 3805 3805 Size: 6388 Bme1580I G_kGCm'C Cuts at: 1790 4449 5605 1790 Size: 2659 1156 2573 Fragments arranged by size: 2659 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 2886 3396 Size: 416 53 83 399 24 952 517 510 Cuts at: 3396 4119 5077 442 Size: 723 958 1753 Fragments arranged by size: 1753 958 952 723 517 510 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2634 2634 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 2597 2718 3618 5048 1239 Size: 1248 110 121 900 1430 2579 Fragments arranged by size: 2579 1430 1248 900 121 110 Bpu10I CC'TnA_GC Cuts at: 3233 3626 3233 Size: 393 5995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4513 5378 5676 4513 Size: 865 298 5225 Fragments arranged by size: 5225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3575 5051 882 Size: 617 2076 1476 2219 Fragments arranged by size: 2219 2076 1476 617 BsaAI yAC'GTr Cuts at: 493 1204 4179 493 Size: 711 2975 2702 Fragments arranged by size: 2975 2702 711 BsaBI GATnn'nnATC Cuts at: 2975 6382 2975 Size: 3407 2981 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 4577 5212 275 Size: 53 83 186 936 2662 382 635 1451 Fragments arranged by size: 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4821 5219 5493 5640 6051 4821 Size: 398 274 147 411 5158 Fragments arranged by size: 5158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3066 3096 1178 Size: 30 165 30 1663 30 4470 Fragments arranged by size: 4470 1663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2751 2790 1186 Size: 1565 39 4784 Fragments arranged by size: 4784 1565 39 BseYI C'CCAG_C Cuts at: 2074 3027 5591 6087 2074 Size: 953 2564 496 2375 Fragments arranged by size: 2564 2375 953 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2730 1163 Size: 573 994 4821 Fragments arranged by size: 4821 994 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2257 2955 3596 4449 4534 5605 2257 Size: 698 641 853 85 1071 3040 Fragments arranged by size: 3040 1071 853 698 641 85 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2257 2955 3124 3217 3596 3707 4449 Size: 399 467 698 169 93 379 111 742 Cuts at: 4449 4534 5605 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 467 399 379 169 111 93 85 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 3743 844 Size: 1256 1643 3489 Fragments arranged by size: 3489 1643 1256 BsrBI CCG'CTC Cuts at: 1394 1823 2134 4275 1394 Size: 429 311 2141 3507 Fragments arranged by size: 3507 2141 429 311 BsrDI GCAATG_nn' Cuts at: 65 1248 3436 4883 5057 65 Size: 1183 2188 1447 174 1396 Fragments arranged by size: 2188 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2211 5032 5219 1700 Size: 110 401 2821 187 2869 Fragments arranged by size: 2869 2821 401 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 2090 3275 794 Size: 1296 1185 3907 Fragments arranged by size: 3907 1296 1185 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 3287 1184 Size: 2103 4285 BstXI CCAn_nnnn'nTGG Cuts at: 2712 2981 2712 Size: 269 6119 BstYI r'GATC_y Cuts at: 1972 2070 2355 2405 2970 3366 3390 3408 3718 Size: 98 285 50 565 396 24 18 310 Cuts at: 3718 4085 4470 4487 5939 6025 6037 6383 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 565 396 385 367 346 310 285 98 86 50 24 18 17 12 Bsu36I CC'TnA_GG Cuts at: 3209 3283 3209 Size: 74 6314 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5836 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2301 487 Size: 683 56 1075 4574 Fragments arranged by size: 4574 1075 683 56 BtsI GCAGTG_nn' Cuts at: 3904 4698 4718 3904 Size: 794 20 5574 Fragments arranged by size: 5574 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2035 5395 809 Size: 507 719 3360 1802 Fragments arranged by size: 3360 1802 719 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 3401 3540 Size: 54 1425 50 275 18 379 1192 139 Cuts at: 3540 3835 4724 6353 8 Size: 295 889 1629 43 Fragments arranged by size: 1629 1425 1192 889 379 295 275 139 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 2603 2662 3294 4318 1353 Size: 771 479 59 632 1024 3423 Fragments arranged by size: 3423 1024 771 632 479 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3594 3594 Size: 6388 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2597 2718 2871 2887 3072 3221 Size: 393 855 110 121 153 16 185 149 Cuts at: 3221 3513 3618 4451 5048 5835 6103 1239 Size: 292 105 833 597 787 268 1524 Fragments arranged by size: 1524 855 833 787 597 393 292 268 185 153 149 121 110 105 16 EcoO109I rG'GnC_Cy Cuts at: 2638 3127 3205 2638 Size: 489 78 5821 Fragments arranged by size: 5821 489 78 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3430 3430 Size: 6388 FspI TGC'GCA Cuts at: 4894 4894 Size: 6388 HaeII r_GCGC'y Cuts at: 1678 5535 1678 Size: 3857 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 2477 2509 3111 Size: 32 135 32 667 32 206 32 602 Cuts at: 3111 3143 5029 5061 5103 5135 1373 Size: 32 1886 32 42 32 2626 Fragments arranged by size: 2626 1886 667 602 206 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 3732 3978 669 Size: 988 281 1234 560 246 3079 Fragments arranged by size: 3079 1234 988 560 281 246 HindIII A'AGCT_T Cuts at: 748 3008 3306 748 Size: 2260 298 3830 Fragments arranged by size: 3830 2260 298 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 2999 3480 3987 2999 Size: 481 507 5400 Fragments arranged by size: 5400 507 481 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5502 5686 1305 Size: 4197 184 2007 Fragments arranged by size: 4197 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3403 3542 10 Size: 54 1425 343 1571 139 2856 Fragments arranged by size: 2856 1571 1425 343 139 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 2916 4346 4705 4864 518 Size: 648 1623 127 1430 359 159 2042 Fragments arranged by size: 2042 1623 1430 648 359 159 127 MspA1I CmG'CkG Cuts at: 1606 2236 3804 4481 5629 5874 1606 Size: 630 1568 677 1148 245 2120 Fragments arranged by size: 2120 1568 1148 677 630 245 NaeI GCC'GGC Cuts at: 2213 2213 Size: 6388 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5836 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NgoMIV G'CCGG_C Cuts at: 2211 2211 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3071 3529 3746 5291 1132 Size: 97 1842 458 217 1545 2229 Fragments arranged by size: 2229 1842 1545 458 217 97 PasI CC'CwG_GG Cuts at: 1348 2059 1348 Size: 711 5677 PciI A'CATG_T Cuts at: 3525 5287 3525 Size: 1762 4626 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6388 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2638 3127 3205 2638 Size: 489 78 5821 Fragments arranged by size: 5821 489 78 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 2525 3958 2525 Size: 1433 4955 PstI C_TGCA'G Cuts at: 830 2629 3740 830 Size: 1799 1111 3478 Fragments arranged by size: 3478 1799 1111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2236 2236 Size: 6388 SacI G_AGCT'C Cuts at: 3596 3596 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SanDI GG'GwC_CC Cuts at: 2638 2638 Size: 6388 SapI GCTCTTCn'nnn_ Cuts at: 2124 2124 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2625 3736 4871 5552 5743 826 Size: 219 1580 1111 1135 681 191 1471 Fragments arranged by size: 1580 1471 1135 1111 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3125 3715 1872 Size: 1253 590 4545 Fragments arranged by size: 4545 1253 590 SmlI C'TyrA_G Cuts at: 820 1017 1943 4492 5393 5655 820 Size: 197 926 2549 901 262 1553 Fragments arranged by size: 2549 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 2227 4312 5 Size: 47 2175 2085 2081 Fragments arranged by size: 2175 2085 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 2065 3138 3827 513 Size: 552 1000 1073 689 3074 Fragments arranged by size: 3074 1073 1000 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2782 4415 4432 4585 4770 5215 325 Size: 1255 1202 1633 17 153 185 445 1498 Fragments arranged by size: 1633 1498 1255 1202 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3463 4634 6346 Size: 274 80 33 51 1443 1486 1171 1712 Cuts at: 6346 96 Size: 138 Fragments arranged by size: 1712 1486 1443 1171 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6348 6380 6348 Size: 32 6356 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6388 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2731 2731 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3123 3713 1870 Size: 1253 590 4545 Fragments arranged by size: 4545 1253 590 XmnI GAAnn'nnTTC Cuts at: 2601 4517 2601 Size: 1916 4472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI