(Circular) (Six-base) MAPSORT of: pFN21AB5535.seq Check: 9194 from: 1 to: 6094 pFN21AB5535 6094 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2092 2092 Size: 6094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2821 3437 1937 Size: 884 616 4594 Fragments arranged by size: 4594 884 616 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 4157 5541 5809 1632 Size: 560 1965 1384 268 1917 Fragments arranged by size: 1965 1917 1384 560 268 AflII C'TTAA_G Cuts at: 820 1017 2471 820 Size: 197 1454 4443 Fragments arranged by size: 4443 1454 197 AflIII A'CryG_T Cuts at: 1203 4993 5970 1203 Size: 3790 977 1327 Fragments arranged by size: 3790 1327 977 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6094 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 2215 2984 3257 3837 5409 2215 Size: 769 273 580 1572 2900 Fragments arranged by size: 2900 1572 769 580 273 ApaI G_GGCC'C Cuts at: 2019 3111 2019 Size: 1092 5002 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 2476 3403 3629 3781 5890 1493 Size: 983 927 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 927 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 3419 1870 Size: 73 1476 4545 Fragments arranged by size: 4545 1476 73 BamHI G'GATC_C Cuts at: 3324 3424 3791 3324 Size: 100 367 5627 Fragments arranged by size: 5627 367 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 2114 3293 3415 4870 Size: 325 217 627 276 51 1179 122 1455 Cuts at: 4870 618 Size: 1842 Fragments arranged by size: 1842 1455 1179 627 325 276 217 122 51 BanII G_rGCy'C Cuts at: 1391 2019 2138 3111 3413 1391 Size: 628 119 973 302 4072 Fragments arranged by size: 4072 973 628 302 119 BbeI G_GCGC'C Cuts at: 2067 2067 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2116 3015 928 Size: 971 217 899 4007 Fragments arranged by size: 4007 971 899 217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2066 2100 4285 4319 1903 Size: 34 129 34 2185 34 3678 Fragments arranged by size: 3678 2185 129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2212 3986 5196 2212 Size: 1774 1210 3110 Fragments arranged by size: 3110 1774 1210 BclI T'GATC_A Cuts at: 1548 2515 1548 Size: 967 5127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4705 136 Size: 107 122 71 631 605 3033 1525 Fragments arranged by size: 3033 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 2019 2117 3111 3296 4155 5311 1790 Size: 229 98 994 185 859 1156 2573 Fragments arranged by size: 2573 1156 994 859 229 185 98 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2382 2413 3825 Size: 416 53 83 399 24 965 31 1412 Cuts at: 3825 4783 442 Size: 958 1753 Fragments arranged by size: 1753 1412 965 958 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2734 4754 1239 Size: 883 612 2020 2579 Fragments arranged by size: 2579 2020 883 612 Bpu10I CC'TnA_GC Cuts at: 2852 2852 Size: 6094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2874 3110 4219 5084 5382 2874 Size: 236 1109 865 298 3586 Fragments arranged by size: 3586 1109 865 298 236 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3180 4757 882 Size: 617 1681 1577 2219 Fragments arranged by size: 2219 1681 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 3901 4283 4918 Size: 53 83 186 936 531 1837 382 635 Cuts at: 4918 275 Size: 1451 Fragments arranged by size: 1837 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2417 3321 4527 4925 5199 5346 5757 2417 Size: 904 1206 398 274 147 411 2754 Fragments arranged by size: 2754 1206 904 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2605 2635 2855 2885 2974 Size: 30 165 30 1202 30 220 30 89 Cuts at: 2974 3004 1178 Size: 30 4268 Fragments arranged by size: 4268 1202 220 165 89 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3019 1186 Size: 1833 4261 BseYI C'CCAG_C Cuts at: 2012 2110 2148 3288 3300 5297 5793 2012 Size: 98 38 1140 12 1997 496 2313 Fragments arranged by size: 2313 1997 1140 496 98 38 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2077 2640 1163 Size: 573 341 563 4617 Fragments arranged by size: 4617 573 563 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3544 4305 5333 1540 Size: 275 186 1543 761 1028 2301 Fragments arranged by size: 2301 1543 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4155 4240 5311 4155 Size: 85 1071 4938 Fragments arranged by size: 4938 1071 85 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2117 2138 3111 3296 3413 4155 Size: 399 229 98 21 973 185 117 742 Cuts at: 4155 4240 5311 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 973 742 399 229 185 117 98 85 21 BspEI T'CCGG_A Cuts at: 3321 5757 3321 Size: 2436 3658 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2092 2115 2662 3449 844 Size: 1248 23 547 787 3489 Fragments arranged by size: 3489 1248 787 547 23 BsrBI CCG'CTC Cuts at: 1394 1823 3981 1394 Size: 429 2158 3507 Fragments arranged by size: 3507 2158 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3076 4589 4763 65 Size: 1183 1828 1513 174 1396 Fragments arranged by size: 1828 1513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3296 4738 4925 1700 Size: 110 1486 1442 187 2869 Fragments arranged by size: 2869 1486 1442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6094 BstBI TT'CG_AA Cuts at: 2752 4898 2752 Size: 2146 3948 BstEII G'GTnAC_C Cuts at: 1184 2344 1184 Size: 1160 4934 BstXI CCAn_nnnn'nTGG Cuts at: 2706 2945 3176 2706 Size: 239 231 5624 Fragments arranged by size: 5624 239 231 BstYI r'GATC_y Cuts at: 1972 3162 3324 3424 3791 4176 4193 5645 5731 Size: 1190 162 100 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1190 385 367 346 162 100 86 17 12 BstZ17I GTA'TAC Cuts at: 2822 2822 Size: 6094 Bsu36I CC'TnA_GG Cuts at: 2859 2859 Size: 6094 BtgI C'CryG_G Cuts at: 513 1065 2004 3111 513 Size: 552 939 1107 3496 Fragments arranged by size: 3496 1107 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2169 487 Size: 683 56 943 4412 Fragments arranged by size: 4412 943 683 56 BtsI GCAGTG_nn' Cuts at: 3372 3610 4404 4424 3372 Size: 238 794 20 5042 Fragments arranged by size: 5042 794 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2623 3541 Size: 54 1425 50 275 18 418 375 918 Cuts at: 3541 4430 6059 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 918 889 418 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 4024 1353 Size: 972 1699 3423 Fragments arranged by size: 3423 1699 972 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2192 2734 4157 4754 5541 5809 Size: 393 490 70 542 1423 597 787 268 Cuts at: 5809 1239 Size: 1524 Fragments arranged by size: 1524 1423 787 597 542 490 393 268 70 EcoO109I rG'GnC_Cy Cuts at: 2016 2016 Size: 6094 EcoRI G'AATT_C Cuts at: 2476 3403 3781 2476 Size: 927 378 4789 Fragments arranged by size: 4789 927 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3207 3207 Size: 6094 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2067 5241 1678 Size: 389 3174 2531 Fragments arranged by size: 3174 2531 389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2855 2887 3239 3271 4735 Size: 32 135 32 1283 32 352 32 1464 Cuts at: 4735 4767 4809 4841 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 1283 352 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2458 3438 3684 669 Size: 988 281 223 297 980 246 3079 Fragments arranged by size: 3079 988 980 297 281 246 223 HindIII A'AGCT_T Cuts at: 748 2961 3086 748 Size: 2213 125 3756 Fragments arranged by size: 3756 2213 125 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 2063 2063 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3351 3693 3351 Size: 342 5752 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 2625 10 Size: 54 1425 343 418 375 3479 Fragments arranged by size: 3479 1425 418 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 4052 4411 4570 518 Size: 648 2199 687 359 159 2042 Fragments arranged by size: 2199 2042 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2142 2775 3288 3510 4187 5335 5580 1606 Size: 536 633 513 222 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 633 536 513 245 222 NarI GG'CG_CC Cuts at: 2064 2064 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 3111 513 Size: 552 939 1107 3496 Fragments arranged by size: 3496 1107 939 552 NdeI CA'TA_TG Cuts at: 387 2227 387 Size: 1840 4254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 3452 4997 1132 Size: 97 2223 1545 2229 Fragments arranged by size: 2229 2223 1545 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 4846 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 2015 3107 2015 Size: 1092 5002 PstI C_TGCA'G Cuts at: 830 3446 830 Size: 2616 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 2775 3288 2775 Size: 513 5581 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 3442 4577 5258 5449 826 Size: 219 2397 1135 681 191 1471 Fragments arranged by size: 2397 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2065 2065 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2471 2889 3089 4198 5099 5361 Size: 197 926 528 418 200 1109 901 262 Cuts at: 5361 820 Size: 1553 Fragments arranged by size: 1553 1109 926 901 528 418 262 200 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6094 StyI C'CwwG_G Cuts at: 513 1065 2004 2236 2272 2642 3111 3533 513 Size: 552 939 232 36 370 469 422 3074 Fragments arranged by size: 3074 939 552 469 422 370 232 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2364 2368 4121 4138 4291 4476 Size: 1255 417 367 4 1753 17 153 185 Cuts at: 4476 4921 325 Size: 445 1498 Fragments arranged by size: 1753 1498 1255 445 417 367 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4340 6052 96 Size: 274 80 33 51 1443 2363 1712 138 Fragments arranged by size: 2363 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 2605 2637 2974 3006 3367 3399 6054 Size: 32 161 32 337 32 361 32 2655 Cuts at: 6054 6086 2412 Size: 32 2420 Fragments arranged by size: 2655 2420 361 337 161 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2765 2765 Size: 6094 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI